引种珙桐的组织培养研究
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摘要
以引种珙桐一年生的茎段为外植体进行珙桐的组织培养,用不同种类消毒剂对外植体进行消毒,以筛选最佳消毒效果,即0.1%HgCl_2消毒15 min,存活率达到61.12%;通过对基本培养基的筛选,发现Woody Plant Medium(WPM)基本培养基最适于珙桐生长;诱导不定芽的最佳培养基为WPM+IBA 0.2 mg/L+TDZ 0.5 mg/L+GA_3 0.5 mg/L,增殖系数为3.33.试验构建了用芽来繁殖不定芽的体系,但增殖系数有待提高,在组织过程中,褐化问题和组培苗的生根有待进一步研究.
The stem segment from one-year-old branch of introduced Davidia involucrata was used as explants to study its micro-propagation technique in this paper.For explants disinfection,the best effect was obtained when the explants were treated with 0.1% HgCl_2 for 15 min.and the survival rate was 61.12%.To induce adventitious bud,the basic medium and the combination of plant regulator were screened based on orthogonal experiment design.The results showed that Woody Plant Medium(WPM) complemented with IBA 0.2 mg/L+TDZ 0.5 mg/L+GA3 0.5 mg/L was the best medium for the induction of bud and its multiplication coefficient reached 3.33.Although the basic system for bud induction was established,the proliferation rate needs to be improved and the browning problem of the explants and the rooting of the induced bud need to be further studied.
The stem segment from one-year-old branch of introduced Davidia involucrata was used as explants to study its micro-propagation technique in this paper.For explants disinfection,the best effect was obtained when the explants were treated with 0.1% HgCl_2 for 15 min.and the survival rate was 61.12%.To induce adventitious bud,the basic medium and the combination of plant regulator were screened based on orthogonal experiment design.The results showed that Woody Plant Medium(WPM) complemented with IBA 0.2 mg/L+TDZ 0.5 mg/L+GA3 0.5 mg/L was the best medium for the induction of bud and its multiplication coefficient reached 3.33.Although the basic system for bud induction was established,the proliferation rate needs to be improved and the browning problem of the explants and the rooting of the induced bud need to be further studied.
引文
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[17] 罗世家.珙桐组织培养研究 [J].林业科技,2006,31(4):3-4.
[18] 罗世家,周光来,王建明.珙桐芽体组织培养研究 [J].湖北民族学院学报:自然科学版,2003,21(4):3-4.
[19] 郭文久.珙桐的外植体选择及组织快繁启动 [J].农学学报,2016,6(2):92-95.
[20] 崔堂兵,郭勇,张长远.植物组织培养中褐变现象的产生机理及克服方法 [J].广东农业科学,2001(3):16-18.
[21] 王金祥,李玲,潘瑞炽.高等植物中赤霉素的生物合成及其调控 [J].植物生理学通讯,2002,38(1):1-8.
[22] 邹利娟,吴庆贵,苏智先.珙桐快速繁殖技术研究 [J].绵阳师范学院学报,2011,30(5):62-66.
[23] 徐晓峰,黄学林.TDZ:一种有效的植物生长调节剂 [J].植物学通报,2003,20(2):227-237.
[2] 胡进耀,苏智先,黎云祥.琪桐生物学研究进展 [J].中国野生植物资源,2003,22(4):15-16.
[3] 禹玉婷,徐刚标,汪晓萍.琪桐研究进展 [J].经济林研究,2006,24(4):92-93.
[4] 张征云,苏智先,申爱英.中国特有植物珙桐的生物学特性濒危原因及保护 [J].淮阴师范学院学报:自然科学版,2003,2(1):66-86.
[5] 司继跃,雷妮娅,司培燕,等.珙桐(Davidia involucrata Baill)研究综述 [J].科学技术与工程,2009,9(13):3718-3725.
[6] Farwig N,Braun C,B?hning-Gaese K.Human disturbance reduces genetic diversity of an endangered tropical tree,Prunus africana (Rosaceae) [J].Conserv Genet,2008,9(2):317-326.
[7] 植爽,胡艳,曹婧.珍稀濒危植物珙桐初代研究 [J].江苏林业科技,2014,41(4):29-32.
[8] 珙桐叶片的脱分化和分化诱导研究 [J].四川大学学报:自然科学版,2010,47(5):1165-1169.
[9] 毛艳萍,苏智先,胡进耀,等.濒危植物珙桐愈伤组织的诱导及悬浮细胞培养初探 [J].武汉植物学研究,2010,28(4):510-515.
[10] 方志荣,蔡光泽.珙桐未成熟胚的萌发及植株再生的研究 [J].西昌学院学报:自然科学版,2015,29(1):7-10.
[11] 李月琴,雷泞菲,林莎,等.濒危植物珙桐的组织培养技术研究 [J].安徽农业科学,2007,35(18):5369-5370.
[12] 于守超,张秀省,杨重军.紫枝玫瑰组织培养中抑制褐化措施的研究 [J].浙江林业科技,2007,27(5):41-43.
[13] 刘洋,苏淑钗,冷平生,等.阿月浑子外植体褐变抑制方法 [J].果树学报,2007,24(3):393-396.
[14] 邹英宁,李国怀,樊青峰,等.不同抗氧化剂对中国李茎段培养的影响 [J].华中农业大学学报,2006,25(1):84-86.
[15] 喜作喜,曾桢迦,植爽,等.植物组织培养中消毒剂的应用研究进展 [J].内江师范学院学报,2013,28(6):26-29.
[16] 陈灵,饶红欣,彭信海.邓恩桉试管苗细菌污染的防治分析 [J].中南林业科技大学学报,2009,29(5):128-130.
[17] 罗世家.珙桐组织培养研究 [J].林业科技,2006,31(4):3-4.
[18] 罗世家,周光来,王建明.珙桐芽体组织培养研究 [J].湖北民族学院学报:自然科学版,2003,21(4):3-4.
[19] 郭文久.珙桐的外植体选择及组织快繁启动 [J].农学学报,2016,6(2):92-95.
[20] 崔堂兵,郭勇,张长远.植物组织培养中褐变现象的产生机理及克服方法 [J].广东农业科学,2001(3):16-18.
[21] 王金祥,李玲,潘瑞炽.高等植物中赤霉素的生物合成及其调控 [J].植物生理学通讯,2002,38(1):1-8.
[22] 邹利娟,吴庆贵,苏智先.珙桐快速繁殖技术研究 [J].绵阳师范学院学报,2011,30(5):62-66.
[23] 徐晓峰,黄学林.TDZ:一种有效的植物生长调节剂 [J].植物学通报,2003,20(2):227-237.