基于DNA池重测序技术的肉兔HSFs基因多态性分析
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摘要
实验旨在研究肉兔热休克因子(HSFs)基因的遗传多态性并寻找耐热性相关分子标记。基于DNA池重测序技术获得SNPs数据,筛选HSF1(SNP1～SNP3)及HSF4(SNP4)上所有的错义突变,利用直接测序法对齐卡巨型兔、齐兴肉兔、加利福尼亚兔、四川白兔以及闽西南黑兔5个肉兔品种(系)共150个个体进行基因分型。结果发现:SNP1和SNP2完全连锁,SNP3呈假阳性,SNP1、SNP2及SNP4的遗传变异处于中等水平,且在群体间分布差异显著(P<0.05)或极显著(P<0.01);SNP1的GG型与单倍型H3的分布与群体间耐热性能的高低一致,其中耐热性最好的四川白兔和闽西南黑兔在SNP1上为GG纯合体、优势单倍型为H3,耐热性次之的齐兴肉兔和加利福尼亚兔的优势基因型和单倍型分别为GG和H3,而耐热性最差的齐卡巨型兔则分别为GA和H2。以上结果表明,HSF1基因SNP1上的GG型及单倍型H3可能是肉兔重要的耐热分子标记。
引文
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[5]王延久,王长法,鞠志花,等.牛HSF1和HSBP1基因多态性分析[J].中国农业科技导报,2011(3):67-72.
[6]李秋玲,鞠志花,贾祥捷,等.中国荷斯坦牛HSF1基因micro RNA SNPs与耐热性能的相关性研究[J].中国农业科学,2011,44(3):570-578.
[7]Li Q L,Ju Z H,Huang J M,et al.Two novel SNPs in HSF1 gene are associated with thermal tolerance traits in Chinese Holstein cattle[J].DNA Cell Biol,2011,30(4):247-254.
[8]Tanabe M,Sasai N,Nagata K,et al.The mammalian HSF4 gene generates both an activator and a repressor of heat shock genes by alternative splicing[J].J Biol Chem,1999,274(39):27845-27856.
[9]Jaeger A M,Iv C W P,Sistonen L,et al.Structures of HSF2 reveal mechanisms for differential regulation of human heat shock factors[J].Nat Struct Mol Biol,2016,23(2):147-154.
[10]Kawazoe Y,Tanabe M,Sasai N,et al.HSF3 is a major heat shock responsive factor during chicken embryonic development[J].Eur JBiochem,1999,265(2):688-697.
[11]Zhang W W,Kong L N,Zhang X Q,et al.Alteration of HSF3 and HSP70 mRNA expression in the tissues of two chicken breeds during acute heat stress[J].Genet Mol Res,2014,13(4):9787-9794.
[12]Lal S V,Brahma B,Gohain M,et al.Splice variants and seasonal expression of buffalo HSF genes[J].Cell Stress Chaperon,201520(3):545-554.
[13]Marai I F M,Habeeb A A M,Gad A E.Rabbits’productive,reproductive and physiological performance traits as affected by heat stress:a review[J].Livest Prod Sci,2002,78(2):71-90.
[14]赵冰茹,黄锡霞,田可川,等.细毛羊LAMB1基因外显子多态性及其与羊毛纤维直径的关联性分析[J].中国畜牧兽医,2016,43(9):2401-2411.
[15]Li Q L,Zhang Z F,Xia P,et al.A SNP in the 3'-UTR of HSF1 in dairy cattle affects binding of target bta-miR-484[J].Genet Mol Res,2015,14(4):12746-12755.
[16]Berry V,Pontikos N,Moore A,et al.A novel missense mutation in HSF4 causes autosomal-dominant congenital lamellar cataract in a British family[J].Eye,2017,32(4):806-812.
[17]Fujimoto M,Izu H,Seki K,et al.HSF4 is required for normal cell growth and differentiation during mouse lens development[J]Embo J,2004,23(21):4297-4306.
[18]Ke T,Wang Q K,Ji B,et al.Novel HSF4 mutation causes congenital total white cataract in a chinese family[J].Am JOphthalmol,2006,142(2):298-303.
[2]Voellmy R.On mechanisms that control heat shock transcri-ption factor activity in metazoan cells[J].Cell Stress Chape-rones,20049(2):122-133.
[3]Hong S H,Sun H K,Mi A H,et al.Coactivator ASC-2 mediates heat shock factor 1-mediated transactivation dependent on heat shock[J].FEBS Letters,2004,559(1-3):165-170.
[4]Baena M M,Tizioto P C,Baena M M,et al.HSF1 and HSPA6 as functional candidate genes associated with heat tolerance in Angus cattle[J].Rev Bras Zootecn,2018,47:1-7.
[5]王延久,王长法,鞠志花,等.牛HSF1和HSBP1基因多态性分析[J].中国农业科技导报,2011(3):67-72.
[6]李秋玲,鞠志花,贾祥捷,等.中国荷斯坦牛HSF1基因micro RNA SNPs与耐热性能的相关性研究[J].中国农业科学,2011,44(3):570-578.
[7]Li Q L,Ju Z H,Huang J M,et al.Two novel SNPs in HSF1 gene are associated with thermal tolerance traits in Chinese Holstein cattle[J].DNA Cell Biol,2011,30(4):247-254.
[8]Tanabe M,Sasai N,Nagata K,et al.The mammalian HSF4 gene generates both an activator and a repressor of heat shock genes by alternative splicing[J].J Biol Chem,1999,274(39):27845-27856.
[9]Jaeger A M,Iv C W P,Sistonen L,et al.Structures of HSF2 reveal mechanisms for differential regulation of human heat shock factors[J].Nat Struct Mol Biol,2016,23(2):147-154.
[10]Kawazoe Y,Tanabe M,Sasai N,et al.HSF3 is a major heat shock responsive factor during chicken embryonic development[J].Eur JBiochem,1999,265(2):688-697.
[11]Zhang W W,Kong L N,Zhang X Q,et al.Alteration of HSF3 and HSP70 mRNA expression in the tissues of two chicken breeds during acute heat stress[J].Genet Mol Res,2014,13(4):9787-9794.
[12]Lal S V,Brahma B,Gohain M,et al.Splice variants and seasonal expression of buffalo HSF genes[J].Cell Stress Chaperon,201520(3):545-554.
[13]Marai I F M,Habeeb A A M,Gad A E.Rabbits’productive,reproductive and physiological performance traits as affected by heat stress:a review[J].Livest Prod Sci,2002,78(2):71-90.
[14]赵冰茹,黄锡霞,田可川,等.细毛羊LAMB1基因外显子多态性及其与羊毛纤维直径的关联性分析[J].中国畜牧兽医,2016,43(9):2401-2411.
[15]Li Q L,Zhang Z F,Xia P,et al.A SNP in the 3'-UTR of HSF1 in dairy cattle affects binding of target bta-miR-484[J].Genet Mol Res,2015,14(4):12746-12755.
[16]Berry V,Pontikos N,Moore A,et al.A novel missense mutation in HSF4 causes autosomal-dominant congenital lamellar cataract in a British family[J].Eye,2017,32(4):806-812.
[17]Fujimoto M,Izu H,Seki K,et al.HSF4 is required for normal cell growth and differentiation during mouse lens development[J]Embo J,2004,23(21):4297-4306.
[18]Ke T,Wang Q K,Ji B,et al.Novel HSF4 mutation causes congenital total white cataract in a chinese family[J].Am JOphthalmol,2006,142(2):298-303.