光交联羧甲基壳聚糖衍生物水凝胶的制备及性能研究
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摘要
以叠氮基团的光耦合反应为交联反应制备了叠氮化羧甲基壳聚糖(AZ-CMCS)水凝胶。利用FT-IR红外光谱、1H-NMR核磁共振谱表征了AZ-CMCS的结构,使用紫外可见分光光度计和流变仪跟踪了AZ-CMCS凝胶的光交联过程,并验证了凝胶的可注射性.使用水接触角测试仪表征了凝胶的亲疏水性.以牛血清白蛋白(BSA)为模型药物研究了水凝胶在不同pH值下的药物释放性能,并研究了人胚胎肺成纤维细胞(MRC-5)在水凝胶上的生长情况.结果表明:通过紫外光照射可快速得到AZ-CMCS水凝胶,反应条件温和,易于控制;AZ-CMCS水凝胶具有较好的可注射性,且具有一定的pH响应性,其能够实现包载药物和药物控释的功能;此外AZ-CMCS水凝胶对细胞无毒,可作为细胞支架,具有较好的应用前景.
The azide carboxymethyl chitosan(AZ-CMCS) hydrogel was prepared by UV-irradiation-induced photocrosslinking reaction which was based on the optical coupling reaction of azide groups. The chemical structure of the AZ-CMCS hydrogel was characterized by FT-IR and 1 H-NMR. The photo-crosslinking process was tracked by a rheometer and a UV-Vis spectrophotometer. The injectable property of the AZ-CMCS hydrogel was verified by a rheometer. The hydrophobicity of the AZ-CMCS hydrogel was assessed by a water contact tester. Bovine serum albumin was used as the model drug to analyze the drug release property of the AZ-CMCS hydrogel at different pH values. The growth condition of human embryonic lung fibroblast(MRC-5) cells on AZ-CMCS scaffolds was also investigated. Results showed that the AZ-CMCS hydrogel can be rapidly prepared by UV irradiation. The reaction conditions are mild and controllable. The AZ-CMCS hydrogel has good injectable properties and pH sensibility. The AZ-CMCS hydrogel can load the model drug and control the drug release behavior. Moreover,the AZ-CMCS hydrogel is noncytotoxic and has a good application prospect as cell scaffold.
The azide carboxymethyl chitosan(AZ-CMCS) hydrogel was prepared by UV-irradiation-induced photocrosslinking reaction which was based on the optical coupling reaction of azide groups. The chemical structure of the AZ-CMCS hydrogel was characterized by FT-IR and 1 H-NMR. The photo-crosslinking process was tracked by a rheometer and a UV-Vis spectrophotometer. The injectable property of the AZ-CMCS hydrogel was verified by a rheometer. The hydrophobicity of the AZ-CMCS hydrogel was assessed by a water contact tester. Bovine serum albumin was used as the model drug to analyze the drug release property of the AZ-CMCS hydrogel at different pH values. The growth condition of human embryonic lung fibroblast(MRC-5) cells on AZ-CMCS scaffolds was also investigated. Results showed that the AZ-CMCS hydrogel can be rapidly prepared by UV irradiation. The reaction conditions are mild and controllable. The AZ-CMCS hydrogel has good injectable properties and pH sensibility. The AZ-CMCS hydrogel can load the model drug and control the drug release behavior. Moreover,the AZ-CMCS hydrogel is noncytotoxic and has a good application prospect as cell scaffold.
引文
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[4]Li G,Tao F R,Wang L P,et al.A facile strategy for preparation of single-chain polymeric nanoparticles by intramolecular photo-crosslinking of azide polymers[J].Polymer,2014,55(16):3696-3702.
[5]Jing Z W,Ma Z W,Li C,et al.Chitosan cross-linked with poly(ethylene glycol)dialdehyde via reductive amination as effective controlled release carriers for oral protein drug delivery[J].Bioorganic and Medicinal Chemistry Letters,2017,27(4):1003-1006.
[6]Choi S K,Goodnow R A,Kalivretenos A,et al.Synthesis of novel and photolabile philanthotoxin analogs:Glutamate_receptor antagonists[J].Tetrahedron,1992,48(23):4793-4822.
[7]Sun C X,Shu K,Wang W,et al.Encapsulation and Controlled release of hydrophilic:Pesticide in shell cross-linked nanocapsules containing aqueous core[J].International Journal of Pharmaceutics,2014,463(1):108-114.
[8]Pescosolido L,Miatto S,Meo C D,et al.Injectable and in situ gelling hydrogels for modified protein release[J].European Biophysics Journal with Biophysics Letters,2010,39(6):903-909.