LC-MS导向分离山壳骨中主成分及其生物活性研究
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摘要
为了解山壳骨(Pseuderanthemum latifolium)的化学成分和生物活性,运用LC-MS联用技术分离得到羽扇豆醇(1)和豆甾醇(2)。体外活性评价结果表明,化合物1和2均具有中等的抗MRSA活性,但不具有神经保护作用。这是首次对山壳骨进行化学成分和生物活性研究,为综合开发与利用山壳骨提供科学依据。
In order to study the chemical constituents from Pseuderanthemum latifolium and their biological activities, two main compounds were isolated from the EaOAc fraction and identified as lupeol(1) and stigmasterol(2) by using LC-MS guided isolation. In vitro activity screening, all these compounds showed moderate anti-MRSA activities but no neuroprotective effects. This is the first report about the chemical constituents from P. latifolium and their biological activities, which would provide scientific basis to the development and utilization of P. latifolium.
In order to study the chemical constituents from Pseuderanthemum latifolium and their biological activities, two main compounds were isolated from the EaOAc fraction and identified as lupeol(1) and stigmasterol(2) by using LC-MS guided isolation. In vitro activity screening, all these compounds showed moderate anti-MRSA activities but no neuroprotective effects. This is the first report about the chemical constituents from P. latifolium and their biological activities, which would provide scientific basis to the development and utilization of P. latifolium.
引文
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[2]TANG H M,LIAO L J.Species of acanthaceae plants and its application in landscape[J].Mod Agric Sci Technol,2013(9):194-195.doi:10.3969/j.issn.1007-5739.2013.09.126.汤慧敏,廖凌娟.爵床科植物种类及其在园林中的应用[J].现代农业科技,2013(9):194-195.doi:10.3969/j.issn.1007-5739.2013.09.126.
[3]Deletis Florae Reipublicae Popularis Sinicae,Agendae Academiae Sinicae Edite.Florae Reipublicae Popularis Sinicae,Tomus 70[M].Beijing:Science Press,2002.中国植物志编委会.中国植物志,第70卷[M].北京:科学出版社,2002.
[4]XIE X,REN Y L,ZHANG H M,et al.Research progress on pharmacological action and application of Rhizolactone[J].Chin J Integr Med Cardio-/Cerebrovasc Dis,2018,16(19):2809-2812.谢璇,任莹璐,张惠敏,等.穿心莲内酯的药理作用和应用研究进展[J].中西医结合心脑血管病杂志,2018,16(19):2809-2812.
[5]WANG F,ZHAI W T,LI Y L,et al.Research progress on new preparations and pharmacokinetic of andrographolide[J].China Pharmac,2013,22(9):1-4.doi:10.3969/j.issn.1006-4931.2013.09.001.王芳,翟文婷,李艳丽,等.穿心莲内酯制剂及药代动力学的研究进展[J].中国药业,2013,22(9):1-4.doi:10.3969/j.issn.1006-4931.2013.09.001.
[6]ZHAI X Y,XIAO W,YANG B,et al.Study on chemical constituents from seed of Oroxylum indicum[J].China J Chin Mat Med,2015,40(15):3013-3016.doi:10.4268/cjcmm20151519.翟翔宇,萧伟,杨彪,等.木蝴蝶种子的化学成分研究[J].中国中药杂志,2015,40(15):3013-3016.doi:10.4268/cjcmm20151519.
[7]EKSHYYAN O,AW T Y.Apoptosis:A key in neurodegenerative disorders[J].Curr Neurovasc Res,2004,1(4):355-371.doi:10.2174/1567202043362018.
[8]OKOUCHI M,EKSHYYAN O,MARACINE M,et al.Neuronal apoptosis in neurodegeneration[J].Antioxid Redox Signal,2007,9(8):1059-1096.doi:10.1089/ars.2007.1511.
[9]CHI H,CHANG H Y,SANG T K.Neuronal cell death mechanisms in major neurodegenerative diseases[J].Int J Mol Sci,2018,19(10):3082.doi:10.3390/ijms19103082.
[10]SWEENEY M D,KISLER K,MONTAGNE A,et al.The role of brain vasculature in neurodegenerative disorders[J].Nat Neurosci,2018,21(10):1318-1331.doi:10.1038/s41593-018-0234-x.
[11]XIAO Y H,WANG J,LI Y,et al.Bacterial resistance surveillance in China:A report from Mohnarin 2004-2005[J].Eur J Clin Microbiol Infect Dis,2008,27(8):697-708.doi:10.1007/s10096-008-0494-6.
[12]SALMENLINNA S,LYYTIKAINEN O,VAINIO A,et al.Human cases of methicillin-resistant Staphylococcus aureus CC398,Finland[J].Emerg Infect Dis,2010,16(10):1626-1629.doi:10.3201/eid1610.091571.
[13]ARêDE P,MILHEIRI?O C,de LENCASTRE H,et al.The antirepressor Mec R2 promotes the proteolysis of the mec A repressor and enables optimal expression ofβ-lactam resistance in MRSA[J].PLo SPathog,2012,8(7):e1002816.doi:10.1371/journal.ppat.1002816
[14]LEWIS P O,HEIL E L,COVERT K L,et al.Treatment strategies for persistent methicillin-resistant Staphylococcus aureus bacteraemia[J].J Clin Pharm Ther,2018,43(5):614-625.doi:10.1111/jcpt.12743.
[15]KOECK M,COMO-SABETTI K,BOXRUD D,et al.Burdens of invasive methicillin-susceptible and methicillin-resistant Staphylococcus aureus disease,minnesota,USA[J].Emerg Infect Dis,2019,25(1):171-174.doi:10.3201/eid2501.181146.
[16]PATEL M.Community-associated meticillin-resistant staphylococcus aureus infections:Epidemiology,recognition and management[J].Drugs,2009,69(6):693-716.doi:10.2165/00003495-200969060-00004.
[17]WELTE T,PLETZ M W.Antimicrobial treatment of nosocomial meticillin-resistant Staphylococcus aureus(MRSA)pneumonia:Current and future options[J].Int J Antimicrob Agents,2010,36(5):391-400.doi:10.1016/j.ijantimicag.2010.06.045.
[18]KEMUNG H M,TAN L T H,KHAN T M,et al.Streptomyces as a prominent resource of future anti-MRSA drugs[J].Front Microbiol,2018,9:2221.doi:10.3389/fmicb.2018.02221.
[19]CHEN S J,POPOVICH J,ZHANG W W,et al.Superior ion release properties and antibacterial efficacy of nanostructured zeolites ionexchanged with zinc,copper,and iron[J].RSC Adv,2018,8(66):37949-37957.doi:10.1039/C8RA06556J.