临床梅毒螺旋体菌株兔感染模型构建
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摘要
目的:构建临床梅毒螺旋体菌株梅毒兔感染模型。方法:收集梅毒患者梅毒螺旋体菌株,制备菌悬液,将悬液注入新西兰兔睾丸并连续分离传代,观察新西兰雄兔临床症状及血清学变化情况。结果:共收集临床梅毒螺旋体菌6株(A-F),接种成功5株—A、C、D、E、F菌株,分别于接种后第28天、7天、14天、32天、35天检测到TPPA转阳。将获得的临床菌株A继续传代,接种6天开始睾丸出现肿胀,平均7.7天TPPA转阳。结论:通过兔睾丸接种的方法,可以成功分离传代临床梅毒螺旋体菌株。
Objective: To establish the animal model of Treponema pallidum strain clinically collected. Methods: The clinically isolated Treponema pallidum strain was prepared for suspension. The suspension was injected into New Zealand rabbit and continuously passaged to next generations. The clinical symptoms of rabbit and serological changes were observed and tested. Results: A total of 6 strains of Treponema pallidum(A-F) were collected and 5 strains(A, C, D, E, F) were successfully inoculated. TPPA conversion was detected on days 28, 7, 14, 32 and 35, respectively. The strain A was passaged and the testicles showed swelling after inoculated strain A for 6 days and positive TPPA conversion after 7.7 days on average. Conclusion: The strain of Treponema pallidum can be successfully isolated clinically and passaged by rabbit testicular inoculation method.
Objective: To establish the animal model of Treponema pallidum strain clinically collected. Methods: The clinically isolated Treponema pallidum strain was prepared for suspension. The suspension was injected into New Zealand rabbit and continuously passaged to next generations. The clinical symptoms of rabbit and serological changes were observed and tested. Results: A total of 6 strains of Treponema pallidum(A-F) were collected and 5 strains(A, C, D, E, F) were successfully inoculated. TPPA conversion was detected on days 28, 7, 14, 32 and 35, respectively. The strain A was passaged and the testicles showed swelling after inoculated strain A for 6 days and positive TPPA conversion after 7.7 days on average. Conclusion: The strain of Treponema pallidum can be successfully isolated clinically and passaged by rabbit testicular inoculation method.
引文
[1] Edmondson DG,Hu B,Norris SJ.Long-term in vitro culture of the syphilis spirochete Treponema pallidum subsp.pallidum[J].Mbio,2018,9(3).doi:1128/mBio.01153-18.
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[3] 于瑞星,尹跃平,韩燕,等.梅毒螺旋体传代接种对兔梅毒模型RPR滴度的影响[J].中国麻风皮肤病杂志,2015,31(5):273-275.
[4] 柯吴坚,吕萍,刘雅慧,等.三种梅毒螺旋体黏附素在梅毒兔模型中转录水平的差异性比较[J].皮肤性病诊疗学杂志,2018,25(1):1-7.
[5] 王琪,尹跃平,于瑞星.梅毒螺旋体感染动物模型的研究进展[J].中国艾滋病性病,2015,21(4):349-350.
[6] 龙福泉,王千秋.梅毒螺旋体感染兔模型的研究[J].国际皮肤性病学杂志,2011,37(3):187-189.
[7] Lukehart SA,Marra CM.Isolation and laboratory maintenance of Treponema pallidum[J].Curr Protoc in Microbiol,2007,12:Unit 12A.1.
[8] Collart P,Franceschini P,Durel P.Experimental rabbit syphilis[J].Br J Vener Dis,1971,47(6):389-400.
[9] Gayet-Ageron A,Lautenschlager S,Ninet B,et al.Sensitivity,specificity and likelihood ratios of PCR in the diagnosis of syphilis:a systematic review and meta-analysis[J].Sex Transm Infect,2013,89(3):251-256.
[10] Tong ML,Zhang HL,Zhu XZ,et al.Re-evaluating the sensitivity of the rabbit infectivity test for Treponema pallidum in modern era[J].Clin Chim Acta,2016,464:136-141.
[11] Wang C,Cheng Y,Liu B,et al.Sensitive detection of Treponema pallidum DNA from the whole blood of patients with syphilis by the nested PCR assay[J].Emerg Microbes Infect,2018,7(1):83.
[12] 马金翠,木柯达斯.梅毒螺旋体Nichols株的液氮冷冻保存及不同传代周期的结果观察[J].中国皮肤性病学杂志,2001,15(5):316.
[13] Chesney AM,Kemp JE.Studies in experimental syphilis[J].J Exp Med,1925,41(4):479-485.
[14] Chesney AM.The influence of the factors of sex,age and method of inoculation upon the course of experimental syphilis in the rabbit[J].J Exp Med,1923,38(5):627-643.
[15] Brause BD,Qualls S,Roberts RB.Testicular Cultivation of Treponema pallidum (Nichols Strain) Facilitated by Sustained-Release Steroid Administration[J].J Clin Microbiol,1979,10(6):937-939.
[16] Adegoke AO,Akanni OE.Survival of Treponema pallidum in banked blood for prevention of Syphilis transmission[J].N Am J Med Sci,2011,3(7):329.
[17] 王琪,尹跃平,龚匡隆.梅毒螺旋体不同接种途径对家兔致病性研究初探[J].中华皮肤科杂志,2015,48(9):621-623,624.
[2] Tong ML,Zhao Q,Liu LL,et al.Whole genome sequence of the Treponema pallidum subsp.pallidum strain Amoy:An Asian isolate highly similar to SS14[J].PLoS One,2017,12(8):e0182768.
[3] 于瑞星,尹跃平,韩燕,等.梅毒螺旋体传代接种对兔梅毒模型RPR滴度的影响[J].中国麻风皮肤病杂志,2015,31(5):273-275.
[4] 柯吴坚,吕萍,刘雅慧,等.三种梅毒螺旋体黏附素在梅毒兔模型中转录水平的差异性比较[J].皮肤性病诊疗学杂志,2018,25(1):1-7.
[5] 王琪,尹跃平,于瑞星.梅毒螺旋体感染动物模型的研究进展[J].中国艾滋病性病,2015,21(4):349-350.
[6] 龙福泉,王千秋.梅毒螺旋体感染兔模型的研究[J].国际皮肤性病学杂志,2011,37(3):187-189.
[7] Lukehart SA,Marra CM.Isolation and laboratory maintenance of Treponema pallidum[J].Curr Protoc in Microbiol,2007,12:Unit 12A.1.
[8] Collart P,Franceschini P,Durel P.Experimental rabbit syphilis[J].Br J Vener Dis,1971,47(6):389-400.
[9] Gayet-Ageron A,Lautenschlager S,Ninet B,et al.Sensitivity,specificity and likelihood ratios of PCR in the diagnosis of syphilis:a systematic review and meta-analysis[J].Sex Transm Infect,2013,89(3):251-256.
[10] Tong ML,Zhang HL,Zhu XZ,et al.Re-evaluating the sensitivity of the rabbit infectivity test for Treponema pallidum in modern era[J].Clin Chim Acta,2016,464:136-141.
[11] Wang C,Cheng Y,Liu B,et al.Sensitive detection of Treponema pallidum DNA from the whole blood of patients with syphilis by the nested PCR assay[J].Emerg Microbes Infect,2018,7(1):83.
[12] 马金翠,木柯达斯.梅毒螺旋体Nichols株的液氮冷冻保存及不同传代周期的结果观察[J].中国皮肤性病学杂志,2001,15(5):316.
[13] Chesney AM,Kemp JE.Studies in experimental syphilis[J].J Exp Med,1925,41(4):479-485.
[14] Chesney AM.The influence of the factors of sex,age and method of inoculation upon the course of experimental syphilis in the rabbit[J].J Exp Med,1923,38(5):627-643.
[15] Brause BD,Qualls S,Roberts RB.Testicular Cultivation of Treponema pallidum (Nichols Strain) Facilitated by Sustained-Release Steroid Administration[J].J Clin Microbiol,1979,10(6):937-939.
[16] Adegoke AO,Akanni OE.Survival of Treponema pallidum in banked blood for prevention of Syphilis transmission[J].N Am J Med Sci,2011,3(7):329.
[17] 王琪,尹跃平,龚匡隆.梅毒螺旋体不同接种途径对家兔致病性研究初探[J].中华皮肤科杂志,2015,48(9):621-623,624.