冷阱捕集-气相色谱-质谱法测定卷烟主流烟气气相物中的苯系物
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摘要
建立了同时选择性检测卷烟主流烟气气相物中7种苯系物(苯、甲苯、乙苯、对-二甲苯、间-二甲苯、邻-二甲苯和苯乙烯)的冷阱捕集-气相色谱-质谱联用(GC/MS)分析方法,并考察了5种不同品牌卷烟样品在深度抽吸与ISO抽吸模式下主流烟气苯系物的释放量。主流烟气气相物中的苯系物经含有内标邻-二甲苯-d10的冷甲醇吸收后在选择离子监测模式下进行GC/MS分析。结果表明:方法的检出限(LOD)和定量限(LOQ)分别为0.006和0.018μg/支,加标回收率为95.8%~110.1%,相对标准偏差(RSD)为1.1%~4.6%。深度抽吸模式下卷烟主流烟气气相物中苯系物的释放量约为ISO模式的两倍。该方法适合用于卷烟主流烟气气相物中7种苯系物释放量测定。
A method for simultaneously selectively determining seven benzenes(benzene, toluene, ethyl benzene, p-xylene, m-xylene, o-xylene and styrene) in mainstream cigarette smoke by cold trap and gas chromatography-mass spectrometry(GC/MS) was developed, and the deliveries of the said analytes in mainstream smoke of five cigarette samples of different brands under intense smoking regime and ISO smoking regime were investigated. The benzenes in the gas phase of mainstream cigarette smoke were trapped with cold methanol containing o-xylene-d10(internal standard), and analyzed by GC/MS under selected ion monitoring mode. The results showed that: 1) The limit of detection and limit of quantification for the seven benzenes were 0.006 and 0.018 μg/cig, respectively. The recoveries ranged from 95.8% to 110.1% with the relative standard deviations(RSDs) of 1.1% to 4.6%. The deliveries of the seven analytes in gas phase of mainstream cigarette smoke under intense smoking regime roughly doubled those under ISO smoking regime. This method is suitable for analyzing the seven benzenes in gas phase of mainstream cigarette smoke.
A method for simultaneously selectively determining seven benzenes(benzene, toluene, ethyl benzene, p-xylene, m-xylene, o-xylene and styrene) in mainstream cigarette smoke by cold trap and gas chromatography-mass spectrometry(GC/MS) was developed, and the deliveries of the said analytes in mainstream smoke of five cigarette samples of different brands under intense smoking regime and ISO smoking regime were investigated. The benzenes in the gas phase of mainstream cigarette smoke were trapped with cold methanol containing o-xylene-d10(internal standard), and analyzed by GC/MS under selected ion monitoring mode. The results showed that: 1) The limit of detection and limit of quantification for the seven benzenes were 0.006 and 0.018 μg/cig, respectively. The recoveries ranged from 95.8% to 110.1% with the relative standard deviations(RSDs) of 1.1% to 4.6%. The deliveries of the seven analytes in gas phase of mainstream cigarette smoke under intense smoking regime roughly doubled those under ISO smoking regime. This method is suitable for analyzing the seven benzenes in gas phase of mainstream cigarette smoke.
引文
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[24]Eschner M S,Selmani I,Gr?ger T M,et al.Online comprehensive two-dimensional characterization of puff-by-puff resolved cigarette smoke by hyphenation of fast gas chromatography to single-photon ionization time-of-flight mass spectrometry:Quantification of hazardous volatile organic compounds[J].Analytical Chemistry,2011,83(17):6619-6627.
[25]Schramm S,CarréV,Scheffler J,et al.Analysis of mainstream and sidestream cigarette smoke particulate matter by laser desorption mass spectrometry[J].Analytical Chemistry,2011,83(1):133-142.
[26]Hatzinikolaou D G,Lagesson V,Stavridou A J,et al.Analysis of the gas phase of cigarette smoke by gas chromatography coupled with UV-diode array detection[J].Analytical Chemistry,2006,78(13):4509-4516.
[2]Moir D,Rickert W S,Levasseur G,et al.A comparison of mainstream and sidestream marijuana and tobacco cigarette smoke produced under two machine smoking conditions[J].Chemical Research in Toxicology,2008,21(2):494-502.
[3]Borgerding M,Klus H.Analysis of complex mixtures-cigarette smoke[J].Experimental and Toxicologic Pathology,2005,57(suppl 1):43-73.
[4]Brunnemann K D,Kagan M R,Cox J E,et al.Analysis of 1,3-butadiene and other selected gas-phase components in cigarette mainstream and sidestream smoke by gas chromatography-mass selective detection[J].Carcinogenesis,1990,11(10):1863-1868.
[5]Omori F,Higashi N,Chida M,et al.Internal standard-based analytical method for tobacco smoke vapor phase components[J].Beitr?ge zur Tabakforschung International,1999,18(4):131-146.
[6]Pankow J F,Luo W,Tavakoli A D,et al.Delivery levels and behavior of 1,3-butadiene,acrylonitrile,benzene,and other toxic volatile organic compounds in mainstream tobacco smoke from two brands of commercial cigarettes[J].Chemical Research in Toxicology,2004,17(6):805-813.
[7]Polzin G M,Kosa-Maines R E,Ashley D L,et al.Analysis of volatile organic compounds in mainstream cigarette smoke[J].Environmental Science&Technology,2007,41(4):1297-1302.
[8]Darrall K G,Figgins J A,Brown R D,et al.Determination of benzene and associated volatile compounds in mainstream cigarette smoke[J].Analyst,1998,123:1095-1101.
[9]侯宏卫,熊巍,姜兴益,等.气相色谱-质谱联用法测定卷烟主流烟气气相物中挥发性有机化合物[J].中国烟草科学,2011,32(5):81-86.
[10]韩冰,王海艳.冷溶剂-气相色谱/质谱联用分析卷烟主流烟气气相成分[J].广东化工,2011(7):148-150.
[11]韩冰,蔡君兰,王冰,等.部分国内外卷烟主流烟气气相和粒相中挥发性、半挥发性化合物的分析[J].中国烟草学报,2011,17(4):1-7.
[12]韩冰,刘惠民,谢复炜,等.卷烟主流烟气中挥发和半挥发性成分分析[J].烟草科技,2009(10):32-40.
[13]Pankow J F,Luo W T,Isabelle L M,et al.Determination of a wide range of volatile organic compounds in ambient air using multisorbent adsorption/thermal desorption and gas chromatography mass spectrometry[J].Analytical Chemistry,1998,70(24):5213-5221.
[14]Singer B C,Hodgson A T,Guevarra K S,et al.Gas-phase organics in environmental tobacco smoke.1.Effects of smoking rate,ventilation,and furnishing level on emission factors[J].Environmental Science&Technology,2002,36(5):846-853.
[15]Dallüge J,van Stee L L P,Xu X,et al.Unravelling the composition of very complex samples by comprehensive gas chromatography coupled to time-of-flight mass spectrometry:Cigarette smoke[J].Journal of Chromatography A,2002,974(1/2):169-184.
[16]Pankow J F,Luo W T,Tavakoli A D,et al.Delivery levels and behavior of 1,3-butadiene,acrylonitrile,benzene,and other toxic volatile organic compounds in mainstream tobacco smoke from two brands of commercial cigarettes[J].Chemical Research in Toxicology,2004,17(6):805-813.
[17]宋金勇,马宇平,王文领,等.吸附热脱附-气相色谱/质谱联用分析卷烟主流烟气气相成分[J].衡阳师范学院学报,2006(3):63-65.
[18]Ye Q.Development of solid-phase microextraction followed by gas chromatography-mass spectrometry for rapid analysis of volatile organic chemicals in mainstream cigarette smoke[J].Journal of Chromatography A,2008,1213(2):239-244.
[19]Pieraccini G,Furlanetto S,Orlandini S,et al.Identification and determination of mainstream and sidestream smoke components in different brands and types of cigarettes by means of solid-phase microextraction-gas chromatography-mass spectrometry[J].Journal of Chromatography A,2008,1180(1/2):138-150.
[20]王健,翁俊桀,贾良元,等.同步辐射光电离质谱法研究卷烟主流烟气气相成分[J].分析化学,2012(7):1048-1052.
[21]Mitschke S,Adam T,Streibel T,et al.Application of time-of-flight mass spectrometry with laser-based photoionization methods for time-resolved on-line analysis of mainstream cigarette smoke[J].Analytical Chemistry,2005,77(8):2288-2296.
[22]Adam T,Mitschke S,Streibel T,et al.Quantitative puff-by-puff-resolved characterization of selected toxic compounds in cigarette mainstream smoke[J].Chemical Research in Toxicology,2006,19(4):511-520.
[23]Mühlberger F,Streibel T,Wieser J,et al.Single photon ionization time-of-flight mass spectrometry with a pulsed electron beam pumped excimer VUV lamp for on-line gas analysis:Setup and first results on cigarette smoke and human breath[J].Analytical Chemistry,2005,77(22):7408-7414.
[24]Eschner M S,Selmani I,Gr?ger T M,et al.Online comprehensive two-dimensional characterization of puff-by-puff resolved cigarette smoke by hyphenation of fast gas chromatography to single-photon ionization time-of-flight mass spectrometry:Quantification of hazardous volatile organic compounds[J].Analytical Chemistry,2011,83(17):6619-6627.
[25]Schramm S,CarréV,Scheffler J,et al.Analysis of mainstream and sidestream cigarette smoke particulate matter by laser desorption mass spectrometry[J].Analytical Chemistry,2011,83(1):133-142.
[26]Hatzinikolaou D G,Lagesson V,Stavridou A J,et al.Analysis of the gas phase of cigarette smoke by gas chromatography coupled with UV-diode array detection[J].Analytical Chemistry,2006,78(13):4509-4516.