HCV早期感染者病毒载量与CD4~+CD25~+Foxp3~+调节性T淋巴细胞相关性研究
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摘要
目的研究丙型肝炎病毒(HCV)早期感染者(抗原阳性、抗体阴性)的HCV病毒载量与CD4+CD25+叉头/翅膀状螺旋转录因子(Foxp3+)调节性T淋巴细胞水平的相关性,分析CD4+CD25+Foxp3+调节性T淋巴细胞在HCV感染中的作用。方法应用实时荧光定量聚合酶链反应(FQ RT-PCR)技术检测22例HCV核心抗原阳性、抗体阴性(即HCV Ag阳性、抗-HCV阴性)的早期感染病例、26例核心抗原阳性、抗体阳性(即HCV Ag阳性、抗-HCV阳性)的慢性HCV感染病例,24例核心抗原阴性、抗体阳性(即HCV Ag阴性、抗-HCV阳性)的既往HCV感染病例及22例健康对照组HCV RNA病毒载量;并用流式细胞技术检测各组外周血有核细胞中的CD4+CD25+Foxp3+调节性T淋巴细胞水平。结果 HCV Ag阳性、抗-HCV阴性组,HCV Ag阳性、抗-HCV阳性组,HCV Ag阴性、抗-HCV阳性组及健康对照组CD4+CD25+Foxp3+调节性T淋巴细胞逐级下降,其中抗原阳性、抗体阴性组[(4.86±2.14)%]高于其他组[分别为(3.74±1.67)%、(2.74±1.27)%、(2.56±1.18)%],差异有统计学意义(t值分别为2.023、4.125、4.402,P<0.05);HCV Ag阳性、抗-HCV阴性组CD4+CD25+Foxp3+调节性T淋巴细胞与HCV RNA病毒载量的对数(copy/mL,Ig)呈正相关(r=0.535,P<0.05)。结论 HCV早期感染者CD4+CD25+Foxp3+调节性T淋巴细胞水平与高病毒调定点相关,CD4+CD25+Foxp3+调节性T淋巴细胞水平可能是加速HCV早期感染的影响因素之一。
Objective To study the correlation between HCV viral load in the early HCV-infected patients(antigen-positive antibody-negative)and CD4+CD25+Foxp3+regulatory T cells,and to know the function of CD4+CD25+Foxp3+regulatory T lymphocytes in the HCV infections.Methods We will use the real-time quantitative polymerase chain reaction(FQ RT-PCR)technique to detect HCV RNA viral load in 22 cases of early infected patients with HCV core positive antigen negative antibody(positive HCV Ag,negative HCV Ab),26 cases of chronic HCV-infected patients with core positive antigen and positive antibody(positive HCV Ag,positive HCV Ab),24 cases of following HCV-infected patients with core negative antigen positive antibody(negative HCV Ag,positive HCV Ab)and 22 cases healthy controls.We also will use flow cytometry technique to detect the level of CD4+CD25+Foxp3+regulatory T lymphocytes of nucleated cells in peripheral blood in each group.Results The CD4+CD25+Foxp3+regulatory T lymphocytes in the group of positive HCV Ag negative HCV Ab,the group of positive HCV Ag positive HCV Ab,the group of negative HCV Ag positive HCV Ab and healthy control group is step-down,of which the group of original positive antibody negative antigen(4.86±2.14)%is higher than other groups(3.74±1.67)%,(2.74±1.27)%,(2.56±1.18)%.The difference is statistically significant(t values were 2.023,4.125,4.402,P<0.05);CD4+CD25+Foxp3+regulatory T lymphocytes and HCV RNA viral load logarithmic(copy/mL,Ig)were positively correlated(r=0.535,P<0.05)in core positive antigen negative antibody group.Conclusion The level of CD4+CD25+Foxp3+regulatory T lymphocytes in HCV early infections is associated with the set-point of high virus.CD4+CD25+Foxp3+regulatory T lymphocytes level may be one of the factors to speed up the early HCV infection.
Objective To study the correlation between HCV viral load in the early HCV-infected patients(antigen-positive antibody-negative)and CD4+CD25+Foxp3+regulatory T cells,and to know the function of CD4+CD25+Foxp3+regulatory T lymphocytes in the HCV infections.Methods We will use the real-time quantitative polymerase chain reaction(FQ RT-PCR)technique to detect HCV RNA viral load in 22 cases of early infected patients with HCV core positive antigen negative antibody(positive HCV Ag,negative HCV Ab),26 cases of chronic HCV-infected patients with core positive antigen and positive antibody(positive HCV Ag,positive HCV Ab),24 cases of following HCV-infected patients with core negative antigen positive antibody(negative HCV Ag,positive HCV Ab)and 22 cases healthy controls.We also will use flow cytometry technique to detect the level of CD4+CD25+Foxp3+regulatory T lymphocytes of nucleated cells in peripheral blood in each group.Results The CD4+CD25+Foxp3+regulatory T lymphocytes in the group of positive HCV Ag negative HCV Ab,the group of positive HCV Ag positive HCV Ab,the group of negative HCV Ag positive HCV Ab and healthy control group is step-down,of which the group of original positive antibody negative antigen(4.86±2.14)%is higher than other groups(3.74±1.67)%,(2.74±1.27)%,(2.56±1.18)%.The difference is statistically significant(t values were 2.023,4.125,4.402,P<0.05);CD4+CD25+Foxp3+regulatory T lymphocytes and HCV RNA viral load logarithmic(copy/mL,Ig)were positively correlated(r=0.535,P<0.05)in core positive antigen negative antibody group.Conclusion The level of CD4+CD25+Foxp3+regulatory T lymphocytes in HCV early infections is associated with the set-point of high virus.CD4+CD25+Foxp3+regulatory T lymphocytes level may be one of the factors to speed up the early HCV infection.
引文
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[7]陈瑜,毛卫林,俞炯,等.肝衰竭患者外周血CD4+CD25+调节性T淋巴细胞水平变化与疗效的相关性研究[J].中华检验医学杂志,2007,30(8):854-857.
[8]彭明喜,张哲,许德义,等.HCV感染者外周血CD4+CD25+Foxp3+调节性T细胞的检测及意义[J].浙江医学,2009,7(2):38-40.
[9]Rehermann B,Nascimbeni M.Immunology of hepatitis B virus and hepatitis C virus infection[J].Nat Rev Immunol,2005,5(3):215-229.
[10]Fontenot JD,Rudensky AY.A well adapted regulatory contrivance:regulatory T cell development and the forkhead family transcription factor Foxp3[J].Nat Immunol,2005,6(4):331-337.
[11]Fazekas B,Landay AL.Regulatory T cells in HIV infection:pathogenic or protective participants in the immune response[J].Aids,2008,22(6):671-683.
[2]Moriishi K,Matsuura Y.Pathogenesis of hepatitis C virus[J].Uirusu,2007,57(2):141-149.
[3]Murphy TJ,Ni Choileain N,Zang Y,et al.CD4+CD25+regulatory T cells control innate immune reactivity after injury[J].J Immunol,2005,174(5):2957-2963.
[4]张子宁,胡斯文,徐俊杰,等.中国HIV早期感染者CD4+CD25+Foxp3+调节性T淋巴细胞变化研究[J].中华检验医学杂志,2011,34(8):712-716.
[5]卿克勤,杨朝国,刘蓉.慢性乙型肝炎患者外周血CD4+CD25+Foxp3+调节性T淋巴细胞变化水平及其与抗病毒疗效的关系[J].检验医学,2013,28(9):811-814.
[6]Elpek KG,Yoleu ES,Franke DD,et al.Ex vivo expansion of CD4+CD25+Foxp3+T regulatory cells based on synergy between IL-2and 4-1BB signaling[J].J Immunol,2007,179(11):7295-7304.
[7]陈瑜,毛卫林,俞炯,等.肝衰竭患者外周血CD4+CD25+调节性T淋巴细胞水平变化与疗效的相关性研究[J].中华检验医学杂志,2007,30(8):854-857.
[8]彭明喜,张哲,许德义,等.HCV感染者外周血CD4+CD25+Foxp3+调节性T细胞的检测及意义[J].浙江医学,2009,7(2):38-40.
[9]Rehermann B,Nascimbeni M.Immunology of hepatitis B virus and hepatitis C virus infection[J].Nat Rev Immunol,2005,5(3):215-229.
[10]Fontenot JD,Rudensky AY.A well adapted regulatory contrivance:regulatory T cell development and the forkhead family transcription factor Foxp3[J].Nat Immunol,2005,6(4):331-337.
[11]Fazekas B,Landay AL.Regulatory T cells in HIV infection:pathogenic or protective participants in the immune response[J].Aids,2008,22(6):671-683.