FOXP3在食管癌中表达规律研究
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摘要
目的:研究FOXP3在食管癌中表达规律。方法:选择2010年1月~2010年10月在我院接受手术治疗的食管鳞癌患者48例,选取所有患者肿瘤组织及正常食管黏膜组织进行免疫组化分析,观察FOXP3在食管癌细胞系Eca-109、正常食管黏膜细胞系Eca-109、人食管癌组织、正常食管黏膜组织的表达,FOXP3在不同年龄、性别、肿瘤分化程度、肿瘤直径、淋巴结转移上的表达。结果:FOXP3在食管癌组织中阳性表达率(85.42%)显著高于正常食管黏膜组织(14.58%),P<0.05。FOXP3在正常细胞系表达为阴性,在食管癌细胞系Eca-109中的表达阳性,FOXP3在正常食管黏膜组织中表达阴性,在食管癌组织中表达阳性。在食管癌组织中,FOXP3阳性表达率和食管癌患者的性别、年龄等不同临床病理特征均无关联(P>0.05),和T分期、TNM分期、肿瘤直径、淋巴结转移、远处转移等存在关联(P<0.05)。结论:食管癌细胞能够表达FOXP3,且其表达水平和食管癌的进展关系密切,可以作为临床诊断及治疗食管癌的重要参考指标。
Objective: Studying the express rule of the FOXP3 in esophageal cancer. Methods: 48 patients with esophageal squamous cell carcinomas undergoing surgery treatment in our hospital were chose from January 2010 to October 2010, all patient's tumor tissue and normal esophageal mucosa tissues were selected for immunohistochemical analysis, Observed the FOXP3 expression in Eca-109, normal esophageal mucosa esophageal cancer cell lines Eca-109, human esophageal cancer tissue and normal esophageal mucosa tissues, surveyed the FOXP3 expression in different age, sex, tumor differentiation, tumor size, lymph node metastasis. Results:The positive expression rate of FOXP3 in esophageal cancer(85.42 %) is significantly higher than that of normal esophageal mucosa tissues(14.58 %), P < 0.05. The FOXP3 expression are negative in normal cell lines and normal esophageal mucosa tissues, are positive in Eca-109 esophageal cancer cell lines and esophageal cancer tissues. In esophageal cancer tissues, FOXP3 positive expression rates are no correlation with different clinical pathological characteristics such as gender, age, there were no correlation(P>0.05), are associated with T stag, TNM stage, tumor size, lymph node metastasis, distant metastasis, such as correlation(P<0.05) in the esophageal cancer patients. Conclusions: Esophageal cancer cells are able to expess the FOXP3, and there is a close relationship between the expression level and the esophageal progress. So it can be used as an important reference index of clinical diagnosis and treatment of esophageal cancer.
Objective: Studying the express rule of the FOXP3 in esophageal cancer. Methods: 48 patients with esophageal squamous cell carcinomas undergoing surgery treatment in our hospital were chose from January 2010 to October 2010, all patient's tumor tissue and normal esophageal mucosa tissues were selected for immunohistochemical analysis, Observed the FOXP3 expression in Eca-109, normal esophageal mucosa esophageal cancer cell lines Eca-109, human esophageal cancer tissue and normal esophageal mucosa tissues, surveyed the FOXP3 expression in different age, sex, tumor differentiation, tumor size, lymph node metastasis. Results:The positive expression rate of FOXP3 in esophageal cancer(85.42 %) is significantly higher than that of normal esophageal mucosa tissues(14.58 %), P < 0.05. The FOXP3 expression are negative in normal cell lines and normal esophageal mucosa tissues, are positive in Eca-109 esophageal cancer cell lines and esophageal cancer tissues. In esophageal cancer tissues, FOXP3 positive expression rates are no correlation with different clinical pathological characteristics such as gender, age, there were no correlation(P>0.05), are associated with T stag, TNM stage, tumor size, lymph node metastasis, distant metastasis, such as correlation(P<0.05) in the esophageal cancer patients. Conclusions: Esophageal cancer cells are able to expess the FOXP3, and there is a close relationship between the expression level and the esophageal progress. So it can be used as an important reference index of clinical diagnosis and treatment of esophageal cancer.
引文
[1]吴杲,魏大中,解明然,等.老年胸段食管鳞癌患者的手术安全性分析[J].中国癌症杂志,2014,24(2):151-156Wu Gao,Wei Da-zhong,Xie Ming-ran,et al.The surgical safety analysis of elderly patients with thoracic esophageal squamous cell carcinoma[J].China Oncology,2014,24(2):151-156
[2]邓新娜,范晓燕,贺丽亚,等.淋巴结转移对胸段食管癌及食管胃交界部癌预后的影响[J].现代肿瘤医学,2014,22(4):816-819Deng Xin-na,Fan Xiao-yan,He Li-ya,et al.Carcinoma of the thoracic esophagus and esophagogastric junction:lymph node metastasis and its impact on prognosis[J].Journal of Modern Oncology,2014,22(4):816-819
[3]Douglas C,Wu Joanna,Hester Satish N,et al.Ex vivo expanded human regulatory T cells can prolong survival of a human islet allograft in a humanized mouse model[J].Wood Transplantation,2013,96(8):707-716
[4]张明慧,郭炜,董稚明,等.食管鳞状细胞癌中FBXO32基因表达及其临床意义[J].临床与实验病理学杂志,2013,29(6):632-636Zhang Ming-hui,Guo Wei,Dong Zhi-ming,et al.Expression of FBXO32 in esophageal squamous cell carcinoma and its clinical significance[J].Chinese journal of clinical and experimental pathology,2013,29(6):632-636
[5]薛强,郝继辉.Foxp3与恶性肿瘤治疗关系的研究进展[J].中国肿瘤临床,2014,41(9):608-610Xue Qiang,Hao Ji-hui.Progress on the relationship between Foxp3and malignant tumor treatment[J].Chinese Journal of Clinical Oncology,2014,41(9):608-610
[6]宋军,邱磊,付海啸,等.胃癌组织中调节性T淋巴细胞浸润与预后的关系[J].中华实验外科杂志,2015,32(01):163-164Song Jun,Qiu Lei,Fu Hai-xiao,et al.Relationship between tumor-infiltrating regulatory T lymphocytes in tumor site and prognosis of gastric carcinoma[J].Chinese Journal of Experimental Surgery,2015,32(01):163-164
[7]Harald,von Boehmer Carolin,Daniel.Therapeutic opportunities for manipulating T(Reg)cells in autoimmunity and cancer[J].Nature reviews Drug discovery,2013,12(1):51-63
[8]鲁常青,李青,王辉,等.协同刺激分子B7-H1、B7-H4和叉状头/翅膀状螺旋转录因子阳性调节性T细胞在胃癌中的表达及其临床意义[J].中华实验外科杂志,2014,31(03):492-494Lu Chang-qing,Li Qing,Wang Hui,et al.Expression and clinical significance of costimulatory molecules B7-H1,B7-H4 and forkhead box P3+regulatory T cells in gastric carcinoma[J].Chinese Journal of Experimental Surgery,2014,31(03):492-494
[9]Helena S,Veronica O,Samuel L.Accumulation of CCR4+CTLA-4hi Foxp3+CD25hi regulatory T cells in colon adenocarcinomas correlate to reduced activation of conventional T cells[J].PLo S One,2012,(02):30695-30699
[10]Liu X,Xu X,Lin X.PTD-h FOXP3 protein acts as an immune regulator to convert human CD4+CD25-T cells to regulatory T-like cells[J].Journal of Cellular Biochemistry,2012,(12):3797-3809
[11]Ohkura N,Hamaguchi M,Morikawa H.T cell receptor stimulation-induced epigenetic changes and foxp3 expression are independent and complementary events required for treg cell development[J].Immunity,2012,(05):785-799
[12]霍莉莉,李慧,魏枫,等.乳腺癌中Fox P3表达与上皮间质转化的相关性及其意义[J].中国肿瘤临床,2014,3:158-161Huo Li-li,Li Hui,Wei Feng,et al.Clinical significance of Fox P3 and the correlation of Fox P3 expression with epithelial-mesenchymal transition in breast cancer[J].Chinese Journal of Clinical Oncology,2014,3:158-161
[13]Afonso J,Longatto-Filho A,Martinho O,et al.Low RKIP expression associates with poor prognosis in bladder cancer patients[J].Virchows Arch,2013,462(4):445-453
[14]罗东,尹杰,柯有力.PEBP4、CR-1在食管癌中表达的临床意义[J].广西医学,2014,36(9):1205-1207Luo Dong,Yin Jie,Ke You-li.Clinical Significance of PEBP4,Cripto-1 Expressions in Esophageal Carcinoma[J].Guangxi Medical Journal,2014,36(9):1205-1207
[15]Zuo T,Liu R,Zhang H,et al.FOXP3 is a novel transcriptional repressor for the breast cancer oncogene SKP2[J].J Clin Invest,2007,117(12):3765-3773
[16]Zuo T,Wang L,Morrison C,et al.FOXP3 is an X-linked breast cancer suppressor gene and an important repressor of the HER-2/Erb B2 oncogene[J].Cell,2007,129(7):1275-1286
[17]Hinz S,Pagerols-Raluy L,Oberg HH,et al.Foxp3 expression in pancreatic carcinoma cells as a novel mechanism of immune evasion in cancer[J].Cancer Res,2007,67(17):8344-8350
[18]Tai X,Erman B,Alag A,et a1.FOXP3 transcription factor is proapoptotic and lethal to developing regulatory T cells unless counterbalanced by cytokine survival signals[J].Immunity,2013,38(6):1116-1128
[19]李敏,陈陆俊,顾文栋,等.叉状头/翅膀状螺旋转录因子在食管癌细胞中的表达及临床意义[J].中华实验外科杂志,2014,31(8):1654-1656Li Min,Chen Lu-jun,Gu Wen-dong,et al.Esophageal cancer cell expressed transcription factor Foxp3 and the analysis of its clinical significance[J].Chinese Journal of Experimental Surgery,2014,31(8):1654-1656
[20]薛磊,陆慧琦,赵学维,等.定量RT-PCR测定食管鳞癌组织中人叉头样转录因子3基因的表达[J].中国胸心血管外科临床杂志,2009,16(3):206-209Xue Lei,Lu Hui-qi,Zhao Xue-wei,et al.Measurement of the Forkhead Box P3 Gene Expression Levels in Esophageal Squamous Cell Carcinoma by Real-time Quantitative Reverse Transcriptionpolymerase Chain Reaction[J].Chinese journal of clinical t horacic and cardiovascular surgery,2009,16(3):206-209
[2]邓新娜,范晓燕,贺丽亚,等.淋巴结转移对胸段食管癌及食管胃交界部癌预后的影响[J].现代肿瘤医学,2014,22(4):816-819Deng Xin-na,Fan Xiao-yan,He Li-ya,et al.Carcinoma of the thoracic esophagus and esophagogastric junction:lymph node metastasis and its impact on prognosis[J].Journal of Modern Oncology,2014,22(4):816-819
[3]Douglas C,Wu Joanna,Hester Satish N,et al.Ex vivo expanded human regulatory T cells can prolong survival of a human islet allograft in a humanized mouse model[J].Wood Transplantation,2013,96(8):707-716
[4]张明慧,郭炜,董稚明,等.食管鳞状细胞癌中FBXO32基因表达及其临床意义[J].临床与实验病理学杂志,2013,29(6):632-636Zhang Ming-hui,Guo Wei,Dong Zhi-ming,et al.Expression of FBXO32 in esophageal squamous cell carcinoma and its clinical significance[J].Chinese journal of clinical and experimental pathology,2013,29(6):632-636
[5]薛强,郝继辉.Foxp3与恶性肿瘤治疗关系的研究进展[J].中国肿瘤临床,2014,41(9):608-610Xue Qiang,Hao Ji-hui.Progress on the relationship between Foxp3and malignant tumor treatment[J].Chinese Journal of Clinical Oncology,2014,41(9):608-610
[6]宋军,邱磊,付海啸,等.胃癌组织中调节性T淋巴细胞浸润与预后的关系[J].中华实验外科杂志,2015,32(01):163-164Song Jun,Qiu Lei,Fu Hai-xiao,et al.Relationship between tumor-infiltrating regulatory T lymphocytes in tumor site and prognosis of gastric carcinoma[J].Chinese Journal of Experimental Surgery,2015,32(01):163-164
[7]Harald,von Boehmer Carolin,Daniel.Therapeutic opportunities for manipulating T(Reg)cells in autoimmunity and cancer[J].Nature reviews Drug discovery,2013,12(1):51-63
[8]鲁常青,李青,王辉,等.协同刺激分子B7-H1、B7-H4和叉状头/翅膀状螺旋转录因子阳性调节性T细胞在胃癌中的表达及其临床意义[J].中华实验外科杂志,2014,31(03):492-494Lu Chang-qing,Li Qing,Wang Hui,et al.Expression and clinical significance of costimulatory molecules B7-H1,B7-H4 and forkhead box P3+regulatory T cells in gastric carcinoma[J].Chinese Journal of Experimental Surgery,2014,31(03):492-494
[9]Helena S,Veronica O,Samuel L.Accumulation of CCR4+CTLA-4hi Foxp3+CD25hi regulatory T cells in colon adenocarcinomas correlate to reduced activation of conventional T cells[J].PLo S One,2012,(02):30695-30699
[10]Liu X,Xu X,Lin X.PTD-h FOXP3 protein acts as an immune regulator to convert human CD4+CD25-T cells to regulatory T-like cells[J].Journal of Cellular Biochemistry,2012,(12):3797-3809
[11]Ohkura N,Hamaguchi M,Morikawa H.T cell receptor stimulation-induced epigenetic changes and foxp3 expression are independent and complementary events required for treg cell development[J].Immunity,2012,(05):785-799
[12]霍莉莉,李慧,魏枫,等.乳腺癌中Fox P3表达与上皮间质转化的相关性及其意义[J].中国肿瘤临床,2014,3:158-161Huo Li-li,Li Hui,Wei Feng,et al.Clinical significance of Fox P3 and the correlation of Fox P3 expression with epithelial-mesenchymal transition in breast cancer[J].Chinese Journal of Clinical Oncology,2014,3:158-161
[13]Afonso J,Longatto-Filho A,Martinho O,et al.Low RKIP expression associates with poor prognosis in bladder cancer patients[J].Virchows Arch,2013,462(4):445-453
[14]罗东,尹杰,柯有力.PEBP4、CR-1在食管癌中表达的临床意义[J].广西医学,2014,36(9):1205-1207Luo Dong,Yin Jie,Ke You-li.Clinical Significance of PEBP4,Cripto-1 Expressions in Esophageal Carcinoma[J].Guangxi Medical Journal,2014,36(9):1205-1207
[15]Zuo T,Liu R,Zhang H,et al.FOXP3 is a novel transcriptional repressor for the breast cancer oncogene SKP2[J].J Clin Invest,2007,117(12):3765-3773
[16]Zuo T,Wang L,Morrison C,et al.FOXP3 is an X-linked breast cancer suppressor gene and an important repressor of the HER-2/Erb B2 oncogene[J].Cell,2007,129(7):1275-1286
[17]Hinz S,Pagerols-Raluy L,Oberg HH,et al.Foxp3 expression in pancreatic carcinoma cells as a novel mechanism of immune evasion in cancer[J].Cancer Res,2007,67(17):8344-8350
[18]Tai X,Erman B,Alag A,et a1.FOXP3 transcription factor is proapoptotic and lethal to developing regulatory T cells unless counterbalanced by cytokine survival signals[J].Immunity,2013,38(6):1116-1128
[19]李敏,陈陆俊,顾文栋,等.叉状头/翅膀状螺旋转录因子在食管癌细胞中的表达及临床意义[J].中华实验外科杂志,2014,31(8):1654-1656Li Min,Chen Lu-jun,Gu Wen-dong,et al.Esophageal cancer cell expressed transcription factor Foxp3 and the analysis of its clinical significance[J].Chinese Journal of Experimental Surgery,2014,31(8):1654-1656
[20]薛磊,陆慧琦,赵学维,等.定量RT-PCR测定食管鳞癌组织中人叉头样转录因子3基因的表达[J].中国胸心血管外科临床杂志,2009,16(3):206-209Xue Lei,Lu Hui-qi,Zhao Xue-wei,et al.Measurement of the Forkhead Box P3 Gene Expression Levels in Esophageal Squamous Cell Carcinoma by Real-time Quantitative Reverse Transcriptionpolymerase Chain Reaction[J].Chinese journal of clinical t horacic and cardiovascular surgery,2009,16(3):206-209