不同产地霸王花粗多糖的单糖组成及体外抗氧化活性
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摘要
为明确不同来源霸王花粗多糖(Hylocereus undatus polysaccharides,HUP)的理化性质、单糖组成和体外抗氧化活性,采用水提醇沉法制备HUP,分别以苯酚-硫酸法、考马斯亮蓝法测定HUP的糖和蛋白质含量;柱前衍生化HPLC法分析HUP的单糖组成;以对羟基自由基、超氧阴离子和ABTS的清除作用为指标,评价HUP的体外抗氧化活性。结果表明,12个不同产地的HUP中糖含量为38%～62%,蛋白质含量为1.09%～3.68%;单糖组成上,最多的为半乳糖,其次为阿拉伯糖,最少的为葡萄糖醛酸;HUP具有显著的抗氧化活性,质量浓度为4 mg/mL时,对羟基自由基和ABTS自由基清除率可达到80%,质量浓度为2 mg/mL时,对超氧阴离子自由基清除率可达到80%。研究表明半乳糖和阿拉伯糖在HUP中的比例有望作为其质量控制的指标,HUP具有显著的抗氧化活性,显示其在食品和药品等领域具有良好的开发利用前景。
Crude polysaccharides from Hylocereus undatus(Haw.) Urilt.et.Rose(HUP) of different regions were isolated by water extraction and alcohol precipitation to study their physicochemical properties,monosaccharide compositions and in vitro antioxidant activities.Phenol-sulfuric acid and coomassie brilliant blue methods were respectively applied to determine the contents of saccharides and proteins in HUP.High performance liquid chromatography(HPLC) pre-derivatized with 1-phenyl-3-methyl-5-pyrazolone(PMP) was used to analyze the monosaccharide compositions.The superoxide anion,hydroxyl radical and ABTS radical scavenging activities were assayed to evaluate the in vitro antioxidant activity of HUP.The results demonstrated that the contents of saccharides were 38%-62% and the protein contents were 1.09%-3.68% in HUP from 12 different regions.The most abundant monosaccharide in HUP was galactose,followed by arabinose,and the lowest was glucuronic acid in content.The HUP had significant anti-oxidative activities.The scavenging rate for hydroxyl radical and ABTS radical were 80% at the concentration of 4 mg/mL and the scavenging rate for superoxide anion radical reached 80% at the concentration of 2 mg/mL.In conclusion,the ratio of galactose and arabinose in HUP can potentially be used as an indicator for the quality control.The crude polysaccharides have significant anti-oxidative activities in vitro and good prospects for development and utilization in the fields of food and medicine.
Crude polysaccharides from Hylocereus undatus(Haw.) Urilt.et.Rose(HUP) of different regions were isolated by water extraction and alcohol precipitation to study their physicochemical properties,monosaccharide compositions and in vitro antioxidant activities.Phenol-sulfuric acid and coomassie brilliant blue methods were respectively applied to determine the contents of saccharides and proteins in HUP.High performance liquid chromatography(HPLC) pre-derivatized with 1-phenyl-3-methyl-5-pyrazolone(PMP) was used to analyze the monosaccharide compositions.The superoxide anion,hydroxyl radical and ABTS radical scavenging activities were assayed to evaluate the in vitro antioxidant activity of HUP.The results demonstrated that the contents of saccharides were 38%-62% and the protein contents were 1.09%-3.68% in HUP from 12 different regions.The most abundant monosaccharide in HUP was galactose,followed by arabinose,and the lowest was glucuronic acid in content.The HUP had significant anti-oxidative activities.The scavenging rate for hydroxyl radical and ABTS radical were 80% at the concentration of 4 mg/mL and the scavenging rate for superoxide anion radical reached 80% at the concentration of 2 mg/mL.In conclusion,the ratio of galactose and arabinose in HUP can potentially be used as an indicator for the quality control.The crude polysaccharides have significant anti-oxidative activities in vitro and good prospects for development and utilization in the fields of food and medicine.
引文
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[21] 胡位荣,孙茹,李昭露,等.霸王花水溶性多糖提取工艺及其对羟自由基的清除作用[J].食品科学,2013(14):104-107.
[2] Xu L S,Wang L Z,Zhang Y J.Structure characteristics of a water-soluble polysaccharide purified from dragon fruit(Hylocereus undatus) pulp[J].Carbohydrate Polymers,2016,146:224-230.
[3] 郭浩杰,杨严格,安乐,等.中药多糖的分子修饰及其药理活性研究进展[J].中草药,2015,46(7):1074-1079.
[4] 安晓娟,洪琳,宋红平,等.植物多糖的结构分析及药理活性研究进展[J].中国药学杂志,2012,47(16):1271-1275.
[5] 何慕雪,孟凡成,王春明,等.一种水溶性霸王花多糖的分离纯化及结构鉴定[J].食品科学,2017(18):106-112.
[6] 郭元亨,张利军,曹丽丽,等.植物多糖中单糖组成分析技术的研究进展[J].食品科学,2018,39(1):326-332.
[7] Ballesteros L F,Teixeira J A,Mussatto S I.Extraction of polysaccharides by autohydrolysis of spent coffee grounds and evaluation of their antioxidant activity[J].Carbohydrate Polymers,2017,157:258-266.
[8] 赵大云,杨丽娥,赵仪华,等.苜蓿多糖提取及测定方法的比较[J].上海交通大学学报(农业科学版),2004,22(3):256-260.
[9] Wang Y F,Xian J H,Xi X G,et al.Multi-fingerprint and quality control analysis of tea polysaccharides[J].Carbohydrate Polymers,2013,92(1):583-590.
[10] Song X P,Tang J.Extraction optimization,preliminary characterization and bioactivities in vitro of Ligularia hodgsonii polysaccharides[J].International Journal of Molecular Sciences,2016,17,788;doi:10.3390/ijms17050788.
[11] Jeddou K B,Chaari F,Maktouf S,et al.Structural,functional,and antioxidant properties of water-soluble polysaccharides from potatoes peels[J].Food Chemistry,2016,205:97-105.
[12] Wu X,Jiang W,Lu J,et al.Analysis of the monosaccharide composition of water-soluble polysaccharides from Sargassum fusiforme by high performance liquid chromatography/electrospray ionisation mass spectrometry[J].Food Chemistry,2014,145(7):976-983.
[13] 陈杰,董扬,鲁吉珂,等.3种不同产地灵芝子实体粗多糖体外抗氧化活性比较研究[J].食品工业科技,2016(21):100-104.
[14] 陆漫漫,敬璞.酰基化对花色苷呈色及抗氧化性影响[J].上海交通大学学报(农业科学版),2016(6):44-49.
[15] 陈丽萍,王先敏,李茂星,等.栀子中抗氧化的活性成分研究[J].华西药学杂志,2018(2):179-182.
[16] Yi Y,Zhang Q W,Li S L,et al.Simultaneous quantification of major flavonoids in “Bawanghua”,the edible flower of Hylocereus undatus using pressurised liquid extraction and high performance liquid chromatography[J].Food Chemistry,2012,135(2):528-533.
[17] 陈慧玲,胡飞,温其标.植物多糖中蛋白脱除的研究进展[J].天然产物研究与开发,2008,20:185-189.
[18] 李绍平,吴定涛,赵静.糖谱及其在中药多糖质量控制中的应用[J].中国中药杂志,2015,40(17):3505-3513.
[19] 肖凌云,李海峰,李姣,等.基于高效液相色谱特征指纹图谱的黄芪-枸杞复合多糖的质量控制[J].中国医院药学杂志,2014,34(21):1795-1799.
[20] Deng Y,Chen L X,Han B X,et al.Qualitative and quantitative analysis of specific polysaccharides in dendrobium huoshanense by using saccharide mapping and chromatographic methods[J].Journal of Pharmaceutical and Biomedical Analysis,2016,129:163-171.
[21] 胡位荣,孙茹,李昭露,等.霸王花水溶性多糖提取工艺及其对羟自由基的清除作用[J].食品科学,2013(14):104-107.