球孢白僵菌与苦参碱混配对烟粉虱的毒力与田间防效
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摘要
旨在探讨球孢白僵菌与8种杀虫剂的相容性以及球孢白僵菌与苦参碱混配对温室烟粉虱的防治效果。首先进行8种杀虫剂与球孢白僵菌的相容性试验,然后选择相容性较高的苦参碱与球孢白僵菌的LC_(50)浓度以9∶1、4∶1、1∶1、1∶4和1∶9体积比进行混配,以共毒系数对其毒力进行评价。结果表明:苦参碱与球孢白僵菌的相容性最好,尤其在10倍稀释度下,苦参碱对球孢白僵菌孢子萌发、菌丝生长及产孢抑制率分别为14.44%、10.17%和12.63%。苦参碱与球孢白僵菌体积比为4∶1时,共毒系数为293,增效作用最明显。综上所述,苦参碱与球孢白僵菌在LC_(50)浓度下以4∶1体积比混配具有极显著的增效作用。
This article is to explore the compatibility of Beauveria bassiana with eight insecticides and the control effect of B.bassiana with matrine for Bemisia tabaci,and provides a reference for integrated control of B.tabaci in the greenhouse.Firstly,the compatibility test of eight pesticides with B.bassiana was conducted.In the concrete mixing process,blending proportions between matrine and B.bassiana of LC_(50) are 9∶1,4∶1,1∶1,1∶4 and 1∶9,and its virulence was assessed by the co-toxicity co-efficient.The results showed that the matrine had the highest compatibility with strains.After being diluted 1∶10,the inhibition rate of spore germination,mycelial growth and sporulation of B.bassiana were 14.44%,10.17% and 12.63%,respectively.The highest co-toxicity co-efficient was 293 inmatrine mixed with B.bassiana in the proportion of 4∶1.In summary,matrine and B.bassiana mixed in the ratio of 4∶1 at the concentration of LC_(50)had a significant synergistic effect.
This article is to explore the compatibility of Beauveria bassiana with eight insecticides and the control effect of B.bassiana with matrine for Bemisia tabaci,and provides a reference for integrated control of B.tabaci in the greenhouse.Firstly,the compatibility test of eight pesticides with B.bassiana was conducted.In the concrete mixing process,blending proportions between matrine and B.bassiana of LC_(50) are 9∶1,4∶1,1∶1,1∶4 and 1∶9,and its virulence was assessed by the co-toxicity co-efficient.The results showed that the matrine had the highest compatibility with strains.After being diluted 1∶10,the inhibition rate of spore germination,mycelial growth and sporulation of B.bassiana were 14.44%,10.17% and 12.63%,respectively.The highest co-toxicity co-efficient was 293 inmatrine mixed with B.bassiana in the proportion of 4∶1.In summary,matrine and B.bassiana mixed in the ratio of 4∶1 at the concentration of LC_(50)had a significant synergistic effect.
引文
[1]张芝利,罗晨.我国烟粉虱的发生危害和防治对策[J].植物保护,2001,27(2):25-30.ZHANG ZH L,LUO CH.Occurrence and control of Bemisia tabaci in China[J].Plant Protection,2001,27(2):25-30.
[2]陈连跟.烟粉虱在园林植物上为害及其形态变异[J].上海农学院学报,1997,15(3):186-189.CHEN L G.The damage and morphological variations of Bemisia tabaci(GENNADIUS)on ornamental plants[J].Journal of Shanghai Agriculture College,1997,15(3):186-189.
[3]GENNADIUS P.Disease of tobacco plantations in Trikonia:the aleurodid of tobacco[J].Ellenike Georgia,1889,6(5):1-3.
[4]周尧.中国粉虱名录[J].中国昆虫学,1949,3(4):1-18.ZHOU Y.Chinese Bemisia tabaci list[J].Chinese Entomology,1949,3(4):1-18.
[5]阎凤鸣.粉虱研究概况[J].北京农学院学报,1990(1):104-108.YAN F M.Research overview of Bemisia tabaci[J].Journal of Beijing Agricultural College,1990(1):104-108.
[6]邓业成,徐汉虹.烟粉虱的化学防治及抗药性[J].农药,2001,43(1):1-6.DENG Y CH,XU H H.Chemical control and resistance of Bemisia tabaci[J].Agrochemcals,2001,43(1):1-6.
[7]任顺祥,邱宝利.中国粉虱及其可持续控制[M].广州:广东省出版集团,2008.REN SH X,QIU B L.Whitefly of China and the Sustainable Control[M].Guangzhou:Guangdong Publishing Group,2008.
[8]胡琼波.植物保护案例分析教程[M].北京:中国农业出版社,2015.HU Q B.Case Study Tutorial of Plant Protection[M].Beijing:China Agricultural Press,2015.
[9]袁盛勇,孔琼,李珣,等.球孢白僵菌Bb070817菌株对番石榴实蝇的室内毒力[J].西北农业学报,2013,22(4):196-200.YUAN SH Y,KONG Q,LI X,et al.Virulence determination of Beauveria bassiana Bb070817against Bactrocera correcta(Bezzi)in laboratory[J].Acta Agricultural Boreali-occidentalis Sinica,2013,22(4):196-200.
[10]WRAIGHT S P,CARRUTHERS RI,JARONSKI ST,et al.Evaluation of the entomopathogenic fungi Beauveria bassiana and Paecilomyces fumosoroseus for microbial control of the silverleaf whitefly,Bemisia argentifolii[J].Biological Control,2000,17:203-217.
[11]BOGO M R,ROTA C A,PINTO H,et al.A chitinase encoding gene(chit1gene)from the entomopathogen Metarhizium anisopliae:isolation and characterization of genomic and full-length cDNA[J].Current Microbiology,1998,37(4):221-225.
[12]余素红,曾明森,吴光远.球孢白僵菌的研究应用与展望[J].茶业科学技术,2009(3):8-11.YU S H,ZENG M S,WU G Y.Research,application and prospect of Beauveria bassiana[J].Tea Science and Technology,2009(3):8-11.
[13]姜灵,洪波,王新谱,等.常用杀虫剂与球孢白僵菌的相容性及对温室白粉虱的协同防效[J].植物保护,2018,44(1):199-204.JIANG L,HONG B,WANG X P,et al.Compatibility of chemical pesticides with Beauveria bassiana and synergistic control effect on Trialeurodes vaporariorum[J].Plant Protection,2018,44(1):199-204.
[14]林华峰.虫生真菌研究进展[J].安徽农业大学学报,1998,25(3):251-251.LIN H F.Adances in research on entomology fungi[J].Journal of Anhui Agricultural University,1998,25(3):251-251.
[15]何恒果,李正跃,陈斌,等.虫生真菌对害虫防治的研究与应用[J].云南农业大学学报,2004,19(2):167-173.HE H G,LI ZH Y,CHEN B,et al.Current status in basic and applied research on entomopathogenic fungi for pest management[J].Journal of Yunnan Agricultural University,2004,19(2):167-173.
[16]李英,张云龙,苗路窑,等.一株分离自感病桑天牛的高毒力球孢白僵菌菌株对6种植物病原真菌的拮抗作用研究[J].蚕业科学,2018,44(1):25-31.LI Y,ZHANG Y L,MIAO L Y,et al.Antagonistic effect of a high toxicity Beauveria bassiana strain isolated from infected Apriona germari against six plant pathogens[J].Science of Sericulture,2018,44(1):25-31.
[17]姜立波,赵亚冬,周兴隆,等.常用杀螨剂与顶孢霉Ahy1菌株的相容性及协同作用[J].植物保护,2012,38(5):73-77.JIANG L B,ZHAO Y D,ZHOU X L,et al.Boicompatibility between Acremonium hansfordii and common acaricides and the synergistic effect[J].Plant Protection,2012,38(5):73-77.
[18]王定锋,李良德,李慧玲,等.5种植物源农药与球孢白僵菌相容性研究[J].茶叶学报,2015,56(4):244-248.WANG D F,LI L D,LI H L,et al.Compatibilities between Beauveria bassiana and five botanical pesticides[J].Acta Tea Sinica,2015,56(4):244-248.
[19]熊琦,王旭,朱永敏,等.7种化学杀虫剂与球孢白僵菌TST05菌株的相容性研究[J].植物保护,2012,38(3):108-112.XIONG Q,WANG X,ZHU Y M,et al.Compatibility of seven chemical pesticides with Beauveria bassiana strain TS05[J].Plant Protection,2012,38(3):108-112.
[20]曹伟平,宋健,赵建江,等.球孢白僵菌与11种新型化学杀虫剂的相容性评价[J].中国生物防治学报,2016,32(6):749-755.CAO W P,SONG J,ZHAO J J,et al.Evaluation of compatibility between Beauveria bassiana and 11new chemical insecticides[J].Chinese Journal of Biological Control,2016,32(6):749-755.
[21]张英财,农向群,张泽华,等.18种化学农药与绿僵菌的相容性研究[J].中国生物防治学报,2012,28(2):186-191.ZHANG Y C,NONG X Q,ZHANG Z H,et al.Compatibility of eighteen chemical pesticides with Metarhizium anisopliae[J].Chinese Journal of Biological Control,2012,28(2):186-191.
[22]PURWAR J P,SACHAN G C.Synergistic effect of entomogenous fungi on some insecticides against Bihar hairy caterpillar Spilarctia obliqua(Lepidoptera:Arctiidae)[J].Microbiological Research,2006,161:38-42.
[23]秦长生,徐金柱,谢鹏辉,等.绿僵菌相容性杀虫剂筛选及混用防治椰心叶甲[J].华南农业大学学报,2008(2):44-46.QIN CH SH,XU J ZH,XIE P H,et al.Screening of chemical pesticides compatible with Metarhizium anisopliae for control of Brontispa longissima[J].Journal of South China Agricultural University,2008(2):44-46.
[24]王峰,郑鹏飞,农向群,等.球孢白僵菌与3种农药对萝卜蚜的协同防效效果[J].中国生物防治学报,2017,33(6):752-759.WANG F,ZHENG P F,NONG X Q,et al.Synergistic effect of Beauveria bassiana and three pesticides on Lipahis erysimi[J].Chinese Journal of Biological Control,2017,33(6):752-759.
[25]曹伟平,宋健,冯书亮,等.球孢白僵菌与低剂量化学杀虫剂对小菜蛾的协同增效作用[J].中国生物防治学报,2018,34(3):1-7.CAO W P,SONG J,FENG SH L,et al.Synergetic effects of Beauveria bassiana with low dosage chemical insecticides against Plutella xylostella[J].Chinese Journal of Biological Control,2018,34(3):1-7.
[2]陈连跟.烟粉虱在园林植物上为害及其形态变异[J].上海农学院学报,1997,15(3):186-189.CHEN L G.The damage and morphological variations of Bemisia tabaci(GENNADIUS)on ornamental plants[J].Journal of Shanghai Agriculture College,1997,15(3):186-189.
[3]GENNADIUS P.Disease of tobacco plantations in Trikonia:the aleurodid of tobacco[J].Ellenike Georgia,1889,6(5):1-3.
[4]周尧.中国粉虱名录[J].中国昆虫学,1949,3(4):1-18.ZHOU Y.Chinese Bemisia tabaci list[J].Chinese Entomology,1949,3(4):1-18.
[5]阎凤鸣.粉虱研究概况[J].北京农学院学报,1990(1):104-108.YAN F M.Research overview of Bemisia tabaci[J].Journal of Beijing Agricultural College,1990(1):104-108.
[6]邓业成,徐汉虹.烟粉虱的化学防治及抗药性[J].农药,2001,43(1):1-6.DENG Y CH,XU H H.Chemical control and resistance of Bemisia tabaci[J].Agrochemcals,2001,43(1):1-6.
[7]任顺祥,邱宝利.中国粉虱及其可持续控制[M].广州:广东省出版集团,2008.REN SH X,QIU B L.Whitefly of China and the Sustainable Control[M].Guangzhou:Guangdong Publishing Group,2008.
[8]胡琼波.植物保护案例分析教程[M].北京:中国农业出版社,2015.HU Q B.Case Study Tutorial of Plant Protection[M].Beijing:China Agricultural Press,2015.
[9]袁盛勇,孔琼,李珣,等.球孢白僵菌Bb070817菌株对番石榴实蝇的室内毒力[J].西北农业学报,2013,22(4):196-200.YUAN SH Y,KONG Q,LI X,et al.Virulence determination of Beauveria bassiana Bb070817against Bactrocera correcta(Bezzi)in laboratory[J].Acta Agricultural Boreali-occidentalis Sinica,2013,22(4):196-200.
[10]WRAIGHT S P,CARRUTHERS RI,JARONSKI ST,et al.Evaluation of the entomopathogenic fungi Beauveria bassiana and Paecilomyces fumosoroseus for microbial control of the silverleaf whitefly,Bemisia argentifolii[J].Biological Control,2000,17:203-217.
[11]BOGO M R,ROTA C A,PINTO H,et al.A chitinase encoding gene(chit1gene)from the entomopathogen Metarhizium anisopliae:isolation and characterization of genomic and full-length cDNA[J].Current Microbiology,1998,37(4):221-225.
[12]余素红,曾明森,吴光远.球孢白僵菌的研究应用与展望[J].茶业科学技术,2009(3):8-11.YU S H,ZENG M S,WU G Y.Research,application and prospect of Beauveria bassiana[J].Tea Science and Technology,2009(3):8-11.
[13]姜灵,洪波,王新谱,等.常用杀虫剂与球孢白僵菌的相容性及对温室白粉虱的协同防效[J].植物保护,2018,44(1):199-204.JIANG L,HONG B,WANG X P,et al.Compatibility of chemical pesticides with Beauveria bassiana and synergistic control effect on Trialeurodes vaporariorum[J].Plant Protection,2018,44(1):199-204.
[14]林华峰.虫生真菌研究进展[J].安徽农业大学学报,1998,25(3):251-251.LIN H F.Adances in research on entomology fungi[J].Journal of Anhui Agricultural University,1998,25(3):251-251.
[15]何恒果,李正跃,陈斌,等.虫生真菌对害虫防治的研究与应用[J].云南农业大学学报,2004,19(2):167-173.HE H G,LI ZH Y,CHEN B,et al.Current status in basic and applied research on entomopathogenic fungi for pest management[J].Journal of Yunnan Agricultural University,2004,19(2):167-173.
[16]李英,张云龙,苗路窑,等.一株分离自感病桑天牛的高毒力球孢白僵菌菌株对6种植物病原真菌的拮抗作用研究[J].蚕业科学,2018,44(1):25-31.LI Y,ZHANG Y L,MIAO L Y,et al.Antagonistic effect of a high toxicity Beauveria bassiana strain isolated from infected Apriona germari against six plant pathogens[J].Science of Sericulture,2018,44(1):25-31.
[17]姜立波,赵亚冬,周兴隆,等.常用杀螨剂与顶孢霉Ahy1菌株的相容性及协同作用[J].植物保护,2012,38(5):73-77.JIANG L B,ZHAO Y D,ZHOU X L,et al.Boicompatibility between Acremonium hansfordii and common acaricides and the synergistic effect[J].Plant Protection,2012,38(5):73-77.
[18]王定锋,李良德,李慧玲,等.5种植物源农药与球孢白僵菌相容性研究[J].茶叶学报,2015,56(4):244-248.WANG D F,LI L D,LI H L,et al.Compatibilities between Beauveria bassiana and five botanical pesticides[J].Acta Tea Sinica,2015,56(4):244-248.
[19]熊琦,王旭,朱永敏,等.7种化学杀虫剂与球孢白僵菌TST05菌株的相容性研究[J].植物保护,2012,38(3):108-112.XIONG Q,WANG X,ZHU Y M,et al.Compatibility of seven chemical pesticides with Beauveria bassiana strain TS05[J].Plant Protection,2012,38(3):108-112.
[20]曹伟平,宋健,赵建江,等.球孢白僵菌与11种新型化学杀虫剂的相容性评价[J].中国生物防治学报,2016,32(6):749-755.CAO W P,SONG J,ZHAO J J,et al.Evaluation of compatibility between Beauveria bassiana and 11new chemical insecticides[J].Chinese Journal of Biological Control,2016,32(6):749-755.
[21]张英财,农向群,张泽华,等.18种化学农药与绿僵菌的相容性研究[J].中国生物防治学报,2012,28(2):186-191.ZHANG Y C,NONG X Q,ZHANG Z H,et al.Compatibility of eighteen chemical pesticides with Metarhizium anisopliae[J].Chinese Journal of Biological Control,2012,28(2):186-191.
[22]PURWAR J P,SACHAN G C.Synergistic effect of entomogenous fungi on some insecticides against Bihar hairy caterpillar Spilarctia obliqua(Lepidoptera:Arctiidae)[J].Microbiological Research,2006,161:38-42.
[23]秦长生,徐金柱,谢鹏辉,等.绿僵菌相容性杀虫剂筛选及混用防治椰心叶甲[J].华南农业大学学报,2008(2):44-46.QIN CH SH,XU J ZH,XIE P H,et al.Screening of chemical pesticides compatible with Metarhizium anisopliae for control of Brontispa longissima[J].Journal of South China Agricultural University,2008(2):44-46.
[24]王峰,郑鹏飞,农向群,等.球孢白僵菌与3种农药对萝卜蚜的协同防效效果[J].中国生物防治学报,2017,33(6):752-759.WANG F,ZHENG P F,NONG X Q,et al.Synergistic effect of Beauveria bassiana and three pesticides on Lipahis erysimi[J].Chinese Journal of Biological Control,2017,33(6):752-759.
[25]曹伟平,宋健,冯书亮,等.球孢白僵菌与低剂量化学杀虫剂对小菜蛾的协同增效作用[J].中国生物防治学报,2018,34(3):1-7.CAO W P,SONG J,FENG SH L,et al.Synergetic effects of Beauveria bassiana with low dosage chemical insecticides against Plutella xylostella[J].Chinese Journal of Biological Control,2018,34(3):1-7.