不同浓度EMS对老芒麦种子发芽的影响
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摘要
为确定EMS诱变老芒麦种子的适宜条件,本试验以"川草2号"老芒麦为试验材料,利用不同浓度(0.2%、0.4%、0.6%、0.8%、1.0%、1.2%和1.6%)的EMS溶液处理老芒麦种子不同时间(8h和16h)后,通过分析老芒麦种子发芽情况,得出随着EMS浓度和处理时间的增加,老芒麦的开始发芽日和发芽高峰日推后,发芽势和发芽率下降趋势也愈明显;EMS诱变老芒麦的适宜浓度为0.8%,处理时间为16h。
This study aimed to investigate the effects of induced concentration and time of EMS on seed germination of Elymus sibiricus.Takeing Elymus sibiricus L.cv."chuancao No.2 " seeds as materials,seven different concentrations of EMS(0.2%、0.4%、0.6%、0.8%、1.0%、1.2%和 1.6%) were set and two different treatment time(8 h and 16 h) were used to induce the seeds.The result of seed germination show that with the increases of EMS concentration and time,the dates of germinating beginning and germinating peak were delayed,the downtrend of germination energy and germination percentage were more and more obviously,finally determined the concentration of EMS 0.8% and 16 h was the optimum condition of inducing Elymus sibiricus seeds.
This study aimed to investigate the effects of induced concentration and time of EMS on seed germination of Elymus sibiricus.Takeing Elymus sibiricus L.cv."chuancao No.2 " seeds as materials,seven different concentrations of EMS(0.2%、0.4%、0.6%、0.8%、1.0%、1.2%和 1.6%) were set and two different treatment time(8 h and 16 h) were used to induce the seeds.The result of seed germination show that with the increases of EMS concentration and time,the dates of germinating beginning and germinating peak were delayed,the downtrend of germination energy and germination percentage were more and more obviously,finally determined the concentration of EMS 0.8% and 16 h was the optimum condition of inducing Elymus sibiricus seeds.
引文
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[11]夏曾润,杜凤凤,李偲,张吉宇,刘勇,霍雅馨,孔令芳.紫花苜蓿EMS突变体库的构建和形态学性状鉴定[J].草业学报,2014,23(02):215-222.
[12]云娜,石凤翎,王晓龙,张宇,伊凤艳.EMS诱变对蒙农红豆草出苗和幼苗生长的影响[J].中国草地学报,2014,36(03):5-9.
[13]乔雨,石凤翎,熊梅,张凯旋.EMS诱变对缘毛雀麦和直立型扁蓿豆种子萌发的影响[J].种子,2016,35(03):98-100.
[14]王彩芬,安永平,张文银,马静.水稻种子化学诱变剂EMS浓度筛选研究[J].宁夏农林科技,2011,52(10):22-23.
[2]Laudencia-Chingcuanco D.Isolation and characterization of EMS-induced Dy10 and Ax1high molecular weight glutenin subunit deficient mutant lines of elitehexaploid wheat(Triticum aestivuml.cv.)[J].Journal of Cereal Science,2012,56(2):296-299.
[3]石海春,谭义川,夏伟,李仁飞,余学杰,柯永培.19份玉米EMS诱变系的遗传差异评价[J].华北农学报,2016,31(01):110-116.
[4]孟永杰,陈锋,帅海威,刘卫国,杜俊波,刘江,舒凯,杨文钰.大豆EMS突变体库构建及其M_1代重要农艺性状评价[J].大豆科学,2016,35(01):64-69.
[5]曲高平,孙妍妍,庞红喜,吴强,王发禄,胡胜武.甘蓝型油菜EMS突变体库构建及抗除草剂突变体筛选[J].中国油料作物学报,2014,36(01):25-31.
[6]王允,张幸果,李贺敏,宋佳静,崔党群,殷冬梅.花生EMS诱变后代的农艺性状与品质分析[J].植物遗传资源学报,2015,16(04):914-919.
[7]侯艳,朱子成,朱娜娜,陈克农,栾非时,王学征.EMS诱变西瓜突变体库的构建及表型分析[J].西北植物学报,2016,36(12):2411-2420.
[8]马海新,庞胜群,杨邦杰,闫丽娟,汪斌,刘鹏,龙志林.EMS诱变对加工番茄种子萌发的影响[J].种子,2015,34(05):28-33.
[9]Vicente-Dolera N,Pinillos V,Moya M,et al.An improved method to obtain novel mutants in Cucurbita pepo by pollen viability[J].Scientiahorticulturae,2014,(169):14-19.
[10]陈天龙,王彦荣,王宇,张吉宇,刘志鹏.蒺藜苜蓿EMS诱变突变体库的构建及突变体表型的分析[J].草业科学,2015,32(01):71-77.
[11]夏曾润,杜凤凤,李偲,张吉宇,刘勇,霍雅馨,孔令芳.紫花苜蓿EMS突变体库的构建和形态学性状鉴定[J].草业学报,2014,23(02):215-222.
[12]云娜,石凤翎,王晓龙,张宇,伊凤艳.EMS诱变对蒙农红豆草出苗和幼苗生长的影响[J].中国草地学报,2014,36(03):5-9.
[13]乔雨,石凤翎,熊梅,张凯旋.EMS诱变对缘毛雀麦和直立型扁蓿豆种子萌发的影响[J].种子,2016,35(03):98-100.
[14]王彩芬,安永平,张文银,马静.水稻种子化学诱变剂EMS浓度筛选研究[J].宁夏农林科技,2011,52(10):22-23.