酵母菌高糖耐受机制的研究进展
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摘要
在酒精浓醪发酵生产中,酵母菌在高糖度、高渗透压等恶劣环境下发酵产生乙醇的性能与其耐受机制有密切的联系。该文以酵母菌高糖耐受机制为切入点,介绍了耐高糖酵母菌的来源,高糖胁迫下酵母菌的应激反应,归纳已公开报道的与酵母菌高糖耐受机制可能有关的基因,借鉴真菌及细菌在转录组学与蛋白质组学水平上耐受性能及机制的研究,为基因水平上酵母菌高糖耐受机制的相关研究提供参考。
In the thick mash fermentation of alcohol production, the performance of yeast producing ethanol under the severe environment(such as high sugar, high osmotic pressure) and its tolerance mechanisms were closely connected. Based on sugar tolerance mechanism of yeast as the breakthrough point, the paper introduced the source of the high sugar tolerant yeast, the stress response of yeast under high sugar stress, the genes reported that may related to high sugar tolerance resistance were summarized. Taking the research of fungi and bacteria tolerance performance and mechanism in the transcriptomics and proteomics level as reference, it provided references for the study of high sugar tolerance mechanism of yeast on gene level.
In the thick mash fermentation of alcohol production, the performance of yeast producing ethanol under the severe environment(such as high sugar, high osmotic pressure) and its tolerance mechanisms were closely connected. Based on sugar tolerance mechanism of yeast as the breakthrough point, the paper introduced the source of the high sugar tolerant yeast, the stress response of yeast under high sugar stress, the genes reported that may related to high sugar tolerance resistance were summarized. Taking the research of fungi and bacteria tolerance performance and mechanism in the transcriptomics and proteomics level as reference, it provided references for the study of high sugar tolerance mechanism of yeast on gene level.
引文
[1]赵红梅,刘景武.耐高渗酵母的分离、筛选及鉴定[J].食品研究与开发,2006,27(6):34-37.
[2]朱宝生,刘功良,白卫东,等.耐高糖酵母筛选及其高糖胁迫机制的研究进展[J].中国酿造,2016,35(6):11-14.
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[5]王敏,朱会霞,孙金旭,等.高温酵母的分离及其特性研究[J].中国酿造,2006,25(11):38-41.
[6]ROLLAND F,WINDERICK J,THEVELEIN J.Glucose-sensing and signaling mechanisms in yeast[J].FEMS Yeast Res,2002,2(2):183-201.
[7]GAGIANO M,BAUER F F,PRETORIUS I S.The sensing of nutritional status and the relationship to Filamentous growth in Saccharomyces cerevisiae[J].FEMS Yeast Res,2002,2(4):433-470.
[8]赵红梅,刘景武,张伟.耐高渗酵母的分离、筛选及鉴定[J].食品研究与开发,2006,27(6):34-37.
[9]杜鹃.椴树蜜中产酯耐高糖酵母菌的筛选及发酵饮料研究[D].哈尔滨:哈尔滨商业大学,2015.
[10]徐伟,杜娇,杜鹃,等.东北黑蜂椴树蜜中耐高糖酵母菌分离鉴定及透射电子显微镜观察[J].食品与发酵工业,2016,42(8):51-56.
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[12]杨玉华.锻树和椴树蜂蜜[J].中国蜂业,2013(19):1-2.
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[14]赵硕.耐高渗(高糖)酵母菌株的选育[D].合肥:安徽农业大学,2010.
[15]彭郦,曾新安.高糖胁迫对生长期酿酒酵母生理代谢的影响[J].现代食品科技,2011,27(4):397-399.
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[19]黄广庆.缺失GPD2基因对工业酿酒酵母乙醇发酵的影响[D].广州:暨南大学,2013.
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[22]陈小玲,陈英,陆雁,等.酿酒酵母Mbp1基因缺失突变株耐受性研究[J].基因组学与应用生物学,2016,35(2):378-384.
[23]HONG M E,LEE K S,YU B J,et al.Identification of gene targets eliciting improved alcohol tolerance in Saccharomyces cerevisiae through inverse metabolic engineering[J].J Biotechnol,2010,149(1-2):52.
[24]FADEL M,KEERA A A,MOUAFI F E,et al.High level ethanol from sugar cane molasses by a new thermotolerant Saccharomyces cerevisiae strain in industrial scale[J].Biotechnol Res Int,2013(4):253-286.
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[26]彭素琴,吴群,徐岩.产酱香地衣芽孢杆菌CGMCC 3963耐受特征及基于转录组学的耐受机制分析[J].微生物学通报,2014,41(12):2395-2403.
[27]叶美玲.南极酵母AN5重金属Cu2+胁迫的转录组学研究[D].哈尔滨:哈尔滨工业大学,2015.
[28]谢梦琴.盐胁迫下鲁氏接合酵母产MAP酶条件优化与其耐盐机理的研究[D].长沙:湖南农业大学,2014.
[29]白雪,尉捷,张亚兰,等.基于双向电泳技术的丙酮丁醇梭菌ATCC824不同产物阶段膜蛋白质组比较[J].科学技术与工程,2016,16(35):22-26.
[30]张雪.石耳科真菌干旱耐受机制的蛋白质组学分析[D].哈尔滨:哈尔滨工业大学,2015.
[31]杨景晶,钱小红,蔡耘,等.蛋白质组研究中低丰度蛋白质的富集与鉴定技术[J].生命的化学,2015,35(5):686-690.
[32]罗培.转录因子Jun B调控肌细胞蛋白质合成机制的研究[D].武汉:华中农业大学,2015.
[33]李超,杨佳羽,刘佳,等.藤仓赤霉(Gibberella fujikuroi)分子生物学及发酵工程研究进展[J].工业微生物,2016,46(2):47-55.
[34]方翔,曾伟伟,王庆,等.基因组学、转录组学、蛋白质组学和结构生物学在水产科学中的应用[J].动物医学进展,2015,36(7):108-112.
[35]张春兰,秦孜娟,王桂芝,等.转录组与RNA-Seq技术[J].生物技术通报,2012(12):51-56.
[2]朱宝生,刘功良,白卫东,等.耐高糖酵母筛选及其高糖胁迫机制的研究进展[J].中国酿造,2016,35(6):11-14.
[3]王昌魁,张利莉,贺江舟,等.耐高温耐高糖酵母的筛选与驯化[J].江西农业学报,2008(5):100-101,109.
[4]马立安.耐高温酒精酵母菌驯化与筛选[J].湖北农学院学报,2000,20(1):72-73.
[5]王敏,朱会霞,孙金旭,等.高温酵母的分离及其特性研究[J].中国酿造,2006,25(11):38-41.
[6]ROLLAND F,WINDERICK J,THEVELEIN J.Glucose-sensing and signaling mechanisms in yeast[J].FEMS Yeast Res,2002,2(2):183-201.
[7]GAGIANO M,BAUER F F,PRETORIUS I S.The sensing of nutritional status and the relationship to Filamentous growth in Saccharomyces cerevisiae[J].FEMS Yeast Res,2002,2(4):433-470.
[8]赵红梅,刘景武,张伟.耐高渗酵母的分离、筛选及鉴定[J].食品研究与开发,2006,27(6):34-37.
[9]杜鹃.椴树蜜中产酯耐高糖酵母菌的筛选及发酵饮料研究[D].哈尔滨:哈尔滨商业大学,2015.
[10]徐伟,杜娇,杜鹃,等.东北黑蜂椴树蜜中耐高糖酵母菌分离鉴定及透射电子显微镜观察[J].食品与发酵工业,2016,42(8):51-56.
[11]LIU G L,TAO C L,ZHU B S,et al.Identification of Zygosaccharomyces mellis strains in stored honey and their stress tolerance[J].Food Sci Biotechnol,2016,25(6):1645-1650.
[12]杨玉华.锻树和椴树蜂蜜[J].中国蜂业,2013(19):1-2.
[13]赵硕,李平,王卿.耐高糖酵母菌株的选育及应用[J].安徽农业科学,2010,38(9):4801-4803.
[14]赵硕.耐高渗(高糖)酵母菌株的选育[D].合肥:安徽农业大学,2010.
[15]彭郦,曾新安.高糖胁迫对生长期酿酒酵母生理代谢的影响[J].现代食品科技,2011,27(4):397-399.
[16]李晓军,徐鹏.耐高渗酿酒酵母的代谢流分析[J].食品与生物技术学报,2009,28(3):356-360
[17]陈君.海藻糖合成对谷氨酸棒杆菌ATCC 13032环境耐受性及谷氨酸合成的影响[D].广州:华南理工大学,2010.
[18]张穗生,陈东.一个酿酒酵母呼吸突变株在高糖胁迫下的生理特性研究[J].基因组学与应用生物学,2013,32(5):600-607.
[19]黄广庆.缺失GPD2基因对工业酿酒酵母乙醇发酵的影响[D].广州:暨南大学,2013.
[20]张国英.敲除3-磷酸甘油脱氢酶基因对工业酒精酵母发酵的影响[D].无锡:江南大学,2008.
[21]孟刚,魏爱英.大肠杆菌tdc D基因缺失株的构建及其用于耐高糖L-色氨酸发酵的研究[J].发酵科技通讯,2015,44(2):12-16.
[22]陈小玲,陈英,陆雁,等.酿酒酵母Mbp1基因缺失突变株耐受性研究[J].基因组学与应用生物学,2016,35(2):378-384.
[23]HONG M E,LEE K S,YU B J,et al.Identification of gene targets eliciting improved alcohol tolerance in Saccharomyces cerevisiae through inverse metabolic engineering[J].J Biotechnol,2010,149(1-2):52.
[24]FADEL M,KEERA A A,MOUAFI F E,et al.High level ethanol from sugar cane molasses by a new thermotolerant Saccharomyces cerevisiae strain in industrial scale[J].Biotechnol Res Int,2013(4):253-286.
[25]邱巨香,瞿婧怡,林德祥,等.中蜂蜜与意蜂蜜中的耐高糖酵母菌种鉴定[J].蜜蜂杂志,2015(1):10-11.
[26]彭素琴,吴群,徐岩.产酱香地衣芽孢杆菌CGMCC 3963耐受特征及基于转录组学的耐受机制分析[J].微生物学通报,2014,41(12):2395-2403.
[27]叶美玲.南极酵母AN5重金属Cu2+胁迫的转录组学研究[D].哈尔滨:哈尔滨工业大学,2015.
[28]谢梦琴.盐胁迫下鲁氏接合酵母产MAP酶条件优化与其耐盐机理的研究[D].长沙:湖南农业大学,2014.
[29]白雪,尉捷,张亚兰,等.基于双向电泳技术的丙酮丁醇梭菌ATCC824不同产物阶段膜蛋白质组比较[J].科学技术与工程,2016,16(35):22-26.
[30]张雪.石耳科真菌干旱耐受机制的蛋白质组学分析[D].哈尔滨:哈尔滨工业大学,2015.
[31]杨景晶,钱小红,蔡耘,等.蛋白质组研究中低丰度蛋白质的富集与鉴定技术[J].生命的化学,2015,35(5):686-690.
[32]罗培.转录因子Jun B调控肌细胞蛋白质合成机制的研究[D].武汉:华中农业大学,2015.
[33]李超,杨佳羽,刘佳,等.藤仓赤霉(Gibberella fujikuroi)分子生物学及发酵工程研究进展[J].工业微生物,2016,46(2):47-55.
[34]方翔,曾伟伟,王庆,等.基因组学、转录组学、蛋白质组学和结构生物学在水产科学中的应用[J].动物医学进展,2015,36(7):108-112.
[35]张春兰,秦孜娟,王桂芝,等.转录组与RNA-Seq技术[J].生物技术通报,2012(12):51-56.