摘要
利用PCR-SSCP技术对吉富罗非鱼HSP70基因多态性进行检测,并采用最小二乘法分析多态性与罗非鱼Oreochromis niloticus耐寒性状的相关性。结果发现,依据HSP70基因序列设计的引物HP70-1、HP70-4、HP70-6不同耐寒能力样本中的电泳图具有带型多态性,且该基因多态性与其耐寒性状显著相关。据此推断HSP70基因是潜在的罗非鱼耐寒相关基因。
The polymorphisms of HSP70 in the GIFT tilapia samples were detected by using PCR-SSCP technique and the correlation with cold tolerance capability of GIFT tilapia was analyzed by least square method in this study.The results showed that the electrophoresis diagram of the primer HP70-1,HP70-4,HP70-6 designed according to the HSP70 has the polymorphism of the belt type in different cold tolerance samples.The polymorphism of the gene was significantly correlated with its cold tolerance.Therefore,it is inferred that HSP70 genes are potential cold-resistant genes of tilapia.
引文
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