茄子种质资源苗期耐涝性与SSR标记的关联分析
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摘要
本研究对219份茄子种植资源进行耐涝性鉴定,获得了219份材料的耐涝表型数据。用196个SSR标记对219份茄子种质资源基因组进行扫描,分析219份茄子材料的群体结构及亲缘关系,并采用TASSEL软件的一般线性模型(GLM)方法对标记与性状进行关联分析。最终得到了219份茄子材料的群体结构、亲缘关系以及与茄子耐涝性紧密关联的27个分子标记,为茄子耐涝分子标记辅助育种提供一定参考。
In this paper, the wa terlogging resistance of 219 eggplant materials was identified, and the data of waterlogging tolerance were obtained. The population structure, linkage disequilibrium and genetic relationship of219 eggplant materials were assessed with 196 pairs of simple sequence repeat(SSR) markers. The general linear model method in software of TASSEL was used to analyze the association between SSR markers and waterlogging tolerance trait. Finally, the population structure, genetic relationship and 27 SSR markers closely related to the waterlogging resistance of eggplant were obtained, which provided some reference for eggplant-resistant molecular marker-assisted breeding.
In this paper, the wa terlogging resistance of 219 eggplant materials was identified, and the data of waterlogging tolerance were obtained. The population structure, linkage disequilibrium and genetic relationship of219 eggplant materials were assessed with 196 pairs of simple sequence repeat(SSR) markers. The general linear model method in software of TASSEL was used to analyze the association between SSR markers and waterlogging tolerance trait. Finally, the population structure, genetic relationship and 27 SSR markers closely related to the waterlogging resistance of eggplant were obtained, which provided some reference for eggplant-resistant molecular marker-assisted breeding.
引文
Bailey-Serres J.,and V oesenek L.A.C.J.,2008,Flooding stress:acclimations and genetic diversity,Annual Review of Plant Biology,59:313-339
Burgos S.,Stamp P.,and Schmid J.E.,2001,Agronomic and physiological study of cold and flooding tolerance of spel(Triticum spelta L.)and wheat(Triticum aestivum L.),Molecular Biology of the Cell,12:379A
Chen X.C.,Yang X.,Cheng Y.F.,Xue L.B.,and Cheng X.H.2012,Association analysis for quantitative traits and its application in vegetable crops,Zhongguo Shucai(China Vegetables),(16):1-9(陈秀辰,杨旭,成玉富,薛林宝,陈学好2012,数量性状关联分析及其在蔬菜中的应用,中国蔬菜,(16):1-9)
Colmer T.D.,2003,Long-distant'transport of gases in plants:a perspective on internal aeration and radial oxygen loss from roots,Plant cell and Environment,26:17-36
Hanse M.,Kraft T.,Ganestam S.,S觟ll T.,and Nilsson N.O.2001,Linkage disequilibrium mapping of the bolting gene in sea beet using AFLP markers,Genetics Res.,77(1):61-66
Jiang Y.P.,Ding X.T.,Zhang Z.H.,Li P.B.,and Chen C.H.,2012Effects of waterlogging on growth and photosynthesis of eggplant,Zhongguo Guacai(China Cucurbit and Vegetables),25(5):20-23(姜玉萍,丁小涛,张兆辉,李鹏波,陈春宏,2012,淹水胁迫对茄子生长和光合作用的影响,中国瓜菜,25(5):20-23)
Li H.B.,Vaillancourt R.,Mendham N.,and Zhou M.X.,2008Comparative mapping of quantitative trait loci associated with waterlogging tolerance in barley,(Hordeum vulgare L.)Bmc Genomics,9:401-413
Lu D.D.,2014,Identification of RICE resources and association analysis of resistance genes loci to brown planthopper,Thesis for M.S.,Jiangxi Agricultural University,Supervisor Zhu C.L.,pp.21-27(卢东东,2014,水稻褐飞虱抗性资源鉴定和抗性基因位点的关联分析,硕士学位论文,江西农业大学,导师:朱昌兰,pp.21-27)
Mc Carthy M.I.,Abecasis G.R.,Cardon L.R.,Goldstein D.B.,Little J.,Ioannidis J.P.A.,and Hirschhorn J.N.,2008,Genomewide association studies for complex traits:consensus,uncer tainty and challenges,Nature Review Genetics,9(5):356-369
Qiu F.Z.,Zheng Y.L.,Zhang Z.L.,and Xu S.Z.,2007,Mapping of QTL associated with waterlogging tolerance during the seedling stage in maize,Ann Bot-London,99:1067-1081
Sayama T.,Nakazaki T.,Ishikawa G.,Yagasaki K.,Yamada N.Hirota N.,Hirata K.,Yoshikawa T.,Saito H.,Teraishi M.Okumoto Y.,Tsukiyama T.,and Tanisaka T.,2009,QTLanalysis of seed-flooding tolerance in soybean(Glycine max[L.]Merr.),Plant Science,176(4):514-521
Shi M.Z.,and Zhou B.S.,2006,Research advance in physiological damage of flood and water-logging resistance,Anhui Nongye Kexue(Journal of Anhui Agricultural Sciences),34(2):209-210(时明芝,周保松,2006,植物涝害和耐涝机理研究进展,安徽农业科学,34(2):209-210)
Takeda S.,and Matsuoka M.,2008,Genetic approaches to crop improvement:responding to environmental and population changes,Nature Reviews Genetics,9(6):444-457
Yang X.,Wang L.,Zhang Y.,and Cheng Y.F.,2016,To identify the flooding stress tolerance of eggplants at seedling stage,Redai Zuowu Xuebao(Chinese Journal of Tropical Crops),37(12):2311-2319(杨旭,王露,张宇,成玉富,2016,茄子种质资源苗期耐涝性鉴定,热带作物学报,37(12):2311-2319)
Yu J.M.,and Buckler E.S.,2006,Genetic association mapping and genome organization of maize,Curr.Opin.Biotechnol,17:155-160
Yu W.J.,Zhou H.J.,Zhan D.Y.,Shao M.Y.,Li B.Q.,and Jiang Z.H.,2013,Characteristic research on drought and flood disasters in the Yangtze River Basin,Zaihaixue(Journal of Catastrophology),28(3):42-47(于文金,周鸿渐,占达颖,邵明阳,李北群,江志红,2013,长江流域旱涝灾害特征研究,灾害学,28(3):42-47)
Zhang H.,Wei L.,Miao H.,Zhang T.,and Wang C.,2012,Development and validation of genic-SSR markers in sesame by RNA-seq,BMC Genomics,13:316
Zhang L.,Zeng L.H.,Zhu L.L.,Zhu J.Q.,and Yang X.,2015,Analysis on flooding tolerance of eggplant at seedling stage,Anhui Nongye Kexue(Journal of Anhui Agricultural Sciences),43(22):125-127(张璐,曾令辉,朱丽丽,朱俊桥,杨旭,2015,茄子品种资源苗期耐涝性鉴定研究,安徽农业科学,43(22):125-127)
Zhao X.Y.,2011,Association analysis of SSR markers with oil content in wild Arachis accessions,Thesis for M.S.,Chinese Academy of Agricultural Sciences,Supervi sor:Jiang H.F.,pp.13-19(赵新燕,2011,野生花生含油量与SSR标记的关联分析,硕士学位论文,中国农业科学院,导师:姜慧芳,pp.13-19)
Burgos S.,Stamp P.,and Schmid J.E.,2001,Agronomic and physiological study of cold and flooding tolerance of spel(Triticum spelta L.)and wheat(Triticum aestivum L.),Molecular Biology of the Cell,12:379A
Chen X.C.,Yang X.,Cheng Y.F.,Xue L.B.,and Cheng X.H.2012,Association analysis for quantitative traits and its application in vegetable crops,Zhongguo Shucai(China Vegetables),(16):1-9(陈秀辰,杨旭,成玉富,薛林宝,陈学好2012,数量性状关联分析及其在蔬菜中的应用,中国蔬菜,(16):1-9)
Colmer T.D.,2003,Long-distant'transport of gases in plants:a perspective on internal aeration and radial oxygen loss from roots,Plant cell and Environment,26:17-36
Hanse M.,Kraft T.,Ganestam S.,S觟ll T.,and Nilsson N.O.2001,Linkage disequilibrium mapping of the bolting gene in sea beet using AFLP markers,Genetics Res.,77(1):61-66
Jiang Y.P.,Ding X.T.,Zhang Z.H.,Li P.B.,and Chen C.H.,2012Effects of waterlogging on growth and photosynthesis of eggplant,Zhongguo Guacai(China Cucurbit and Vegetables),25(5):20-23(姜玉萍,丁小涛,张兆辉,李鹏波,陈春宏,2012,淹水胁迫对茄子生长和光合作用的影响,中国瓜菜,25(5):20-23)
Li H.B.,Vaillancourt R.,Mendham N.,and Zhou M.X.,2008Comparative mapping of quantitative trait loci associated with waterlogging tolerance in barley,(Hordeum vulgare L.)Bmc Genomics,9:401-413
Lu D.D.,2014,Identification of RICE resources and association analysis of resistance genes loci to brown planthopper,Thesis for M.S.,Jiangxi Agricultural University,Supervisor Zhu C.L.,pp.21-27(卢东东,2014,水稻褐飞虱抗性资源鉴定和抗性基因位点的关联分析,硕士学位论文,江西农业大学,导师:朱昌兰,pp.21-27)
Mc Carthy M.I.,Abecasis G.R.,Cardon L.R.,Goldstein D.B.,Little J.,Ioannidis J.P.A.,and Hirschhorn J.N.,2008,Genomewide association studies for complex traits:consensus,uncer tainty and challenges,Nature Review Genetics,9(5):356-369
Qiu F.Z.,Zheng Y.L.,Zhang Z.L.,and Xu S.Z.,2007,Mapping of QTL associated with waterlogging tolerance during the seedling stage in maize,Ann Bot-London,99:1067-1081
Sayama T.,Nakazaki T.,Ishikawa G.,Yagasaki K.,Yamada N.Hirota N.,Hirata K.,Yoshikawa T.,Saito H.,Teraishi M.Okumoto Y.,Tsukiyama T.,and Tanisaka T.,2009,QTLanalysis of seed-flooding tolerance in soybean(Glycine max[L.]Merr.),Plant Science,176(4):514-521
Shi M.Z.,and Zhou B.S.,2006,Research advance in physiological damage of flood and water-logging resistance,Anhui Nongye Kexue(Journal of Anhui Agricultural Sciences),34(2):209-210(时明芝,周保松,2006,植物涝害和耐涝机理研究进展,安徽农业科学,34(2):209-210)
Takeda S.,and Matsuoka M.,2008,Genetic approaches to crop improvement:responding to environmental and population changes,Nature Reviews Genetics,9(6):444-457
Yang X.,Wang L.,Zhang Y.,and Cheng Y.F.,2016,To identify the flooding stress tolerance of eggplants at seedling stage,Redai Zuowu Xuebao(Chinese Journal of Tropical Crops),37(12):2311-2319(杨旭,王露,张宇,成玉富,2016,茄子种质资源苗期耐涝性鉴定,热带作物学报,37(12):2311-2319)
Yu J.M.,and Buckler E.S.,2006,Genetic association mapping and genome organization of maize,Curr.Opin.Biotechnol,17:155-160
Yu W.J.,Zhou H.J.,Zhan D.Y.,Shao M.Y.,Li B.Q.,and Jiang Z.H.,2013,Characteristic research on drought and flood disasters in the Yangtze River Basin,Zaihaixue(Journal of Catastrophology),28(3):42-47(于文金,周鸿渐,占达颖,邵明阳,李北群,江志红,2013,长江流域旱涝灾害特征研究,灾害学,28(3):42-47)
Zhang H.,Wei L.,Miao H.,Zhang T.,and Wang C.,2012,Development and validation of genic-SSR markers in sesame by RNA-seq,BMC Genomics,13:316
Zhang L.,Zeng L.H.,Zhu L.L.,Zhu J.Q.,and Yang X.,2015,Analysis on flooding tolerance of eggplant at seedling stage,Anhui Nongye Kexue(Journal of Anhui Agricultural Sciences),43(22):125-127(张璐,曾令辉,朱丽丽,朱俊桥,杨旭,2015,茄子品种资源苗期耐涝性鉴定研究,安徽农业科学,43(22):125-127)
Zhao X.Y.,2011,Association analysis of SSR markers with oil content in wild Arachis accessions,Thesis for M.S.,Chinese Academy of Agricultural Sciences,Supervi sor:Jiang H.F.,pp.13-19(赵新燕,2011,野生花生含油量与SSR标记的关联分析,硕士学位论文,中国农业科学院,导师:姜慧芳,pp.13-19)