中华大节竹耐盐基因Is SOS1的克隆与序列分析
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摘要
通过RACE技术,从中华大节竹根中克隆出一个质膜型Na+/H+逆向转运蛋白基因,并命名为Is SOS1(基因登录号:KC113048)。Is SOS1序列分析结果显示,Is SOS1的全长为3 516 bp,其中开放读码框为3 105 bp,编码了1 035个氨基酸多肽。氨基酸同源性和聚类分析证实,Is SOS1氨基酸序列与拟南芥At SOS1和水稻Os SOS1的氨基酸序列同源性较高,分别为50. 3%和76. 63%,但是与液泡型的Na+/H+逆向转运蛋白氨基酸序列同源性较低。
A full length cDNA( named as Is SOS1) of bamboo( Indosasa sinica) was isolated by RACE approach. Sequence analysis indicated that the full-length cDNA of Is SOS1 was3 516 bp,including an open reading frame( ORF) of 3 105 bp,which encoded a deduced polypeptide of 1 035 amino acids. The deduced protein shared a high degree of homology with At SOS1( 50. 3%) and Os SOS1( 76. 63%).
A full length cDNA( named as Is SOS1) of bamboo( Indosasa sinica) was isolated by RACE approach. Sequence analysis indicated that the full-length cDNA of Is SOS1 was3 516 bp,including an open reading frame( ORF) of 3 105 bp,which encoded a deduced polypeptide of 1 035 amino acids. The deduced protein shared a high degree of homology with At SOS1( 50. 3%) and Os SOS1( 76. 63%).
引文
[1]杜利霞,董宽虎,朱慧森.植物Na+/H+逆向转运蛋白研究进展[J].草原与草坪,2012,32(2):82-86.
[2]马清,包爱科,伍国强,等.质膜Na+/H+逆向转运蛋白与植物耐盐性[J].植物学报,2011,46(02):206-215.
[3]WANG S,LI Z,Rui R,et al. Cloning and Characterization of a Plasma Membrane Na+/H+Antiporter Gene from Cucumis sativus[J]. Russian Journal of Plant Physiology,2013,60(3):330-336.
[4]王红艳,王鸿磊.海滨锦葵耐盐基因KvSOS1的克隆与表达载体构建[J].江苏农业科学,2016,44(10):76-80.
[5]郑琳琳,张慧荣,贺龙梅,等.唐古特白刺质膜Na+/H+逆向转运蛋白基因的克隆与表达分析[J].草业学报,2013,22(4):179-186.
[6]菊花及其近缘种属植物质膜Na+/H+逆向转运蛋白SOS1s基因克隆与功能鉴定[D].南京农业大学,2015.
[7]党振华,郑琳琳,冯智,等.泌盐植物长叶红砂质膜Na+/H+逆向转运蛋白基因(RtSOS1)全长c DNA的克隆及序列分析[J].华北农学报,2013,28(3):1-6.
[8]李荣柱.苏北沿海刚竹属竹子栽培措施探讨[J].江苏林业科技,1987,4:24-28.
[9]张玲菊,徐维坤.白哺鸡竹海岸带引种栽培技术[J].林业科技开发,2002,16(4):43-44.
[10]金川,王月英.观赏竹在沿海地区的开发实践与拓展思考[J].竹子研究汇刊,1998,7(17):51-56.
[11]江泽慧.世界竹藤[M].沈阳:辽宁科学技术出版社,2002,402-404.
[12]金久宏,张关通,俞国雄.竹林带沿海防护林营造技术及其效益[J].防护林科技,1997,9(3):23-25.
[13]李迎春,陈双林.竹子耐盐性研究述评[J].山地农业生物学报,2008,27(5):451-455.
[2]马清,包爱科,伍国强,等.质膜Na+/H+逆向转运蛋白与植物耐盐性[J].植物学报,2011,46(02):206-215.
[3]WANG S,LI Z,Rui R,et al. Cloning and Characterization of a Plasma Membrane Na+/H+Antiporter Gene from Cucumis sativus[J]. Russian Journal of Plant Physiology,2013,60(3):330-336.
[4]王红艳,王鸿磊.海滨锦葵耐盐基因KvSOS1的克隆与表达载体构建[J].江苏农业科学,2016,44(10):76-80.
[5]郑琳琳,张慧荣,贺龙梅,等.唐古特白刺质膜Na+/H+逆向转运蛋白基因的克隆与表达分析[J].草业学报,2013,22(4):179-186.
[6]菊花及其近缘种属植物质膜Na+/H+逆向转运蛋白SOS1s基因克隆与功能鉴定[D].南京农业大学,2015.
[7]党振华,郑琳琳,冯智,等.泌盐植物长叶红砂质膜Na+/H+逆向转运蛋白基因(RtSOS1)全长c DNA的克隆及序列分析[J].华北农学报,2013,28(3):1-6.
[8]李荣柱.苏北沿海刚竹属竹子栽培措施探讨[J].江苏林业科技,1987,4:24-28.
[9]张玲菊,徐维坤.白哺鸡竹海岸带引种栽培技术[J].林业科技开发,2002,16(4):43-44.
[10]金川,王月英.观赏竹在沿海地区的开发实践与拓展思考[J].竹子研究汇刊,1998,7(17):51-56.
[11]江泽慧.世界竹藤[M].沈阳:辽宁科学技术出版社,2002,402-404.
[12]金久宏,张关通,俞国雄.竹林带沿海防护林营造技术及其效益[J].防护林科技,1997,9(3):23-25.
[13]李迎春,陈双林.竹子耐盐性研究述评[J].山地农业生物学报,2008,27(5):451-455.