不同菌属的氟苯尼考耐药特点及floR基因的序列分析
|
摘要
为了解福建宁德地区猪源细菌对氟苯尼考耐药性及耐药基因floR的遗传特性。本研究从宁德地区6个猪场分别采集了50份仔猪粪便样品。从中分离得到氟苯尼考耐药大肠杆菌17株、沙门氏菌2株、肺炎克雷伯菌5株和鲍曼不动杆菌8株。并对其耐药基因floR进行同源性分析。结果显示:宁德地区猪源大肠杆菌、沙门氏菌、肺炎克雷伯菌和鲍曼不动杆菌对氟苯尼考的耐药检出率分别为34%、4%、10%和16%。且所有的分离细菌都呈现了多重耐药性。同源性分析发现不同细菌间的耐药基因floR存在高度同源性。这些结果表明氟苯尼考耐药基因floR广泛存在于不同细菌间,且可在不同细菌间进行水平传播。该研究为氟苯尼考耐药基因floR的广泛传播奠定了一定的理论基础,对临床合理用药提供了依据。
To illuminate the florfenicol resistance of bacteria from swine and genetic characteristics of floR gene in Ningde, Fujian, 50 piglet feces samples were collected from 6 pig farms. 17 strains of florfenicol resistant Escherichia coli, 2 strains of Salmonella, 5 strains of Klebsiella pneumoniae and 8 strains of Acinetobacter baumannii were isolated. And the homology of floR gene was analyzed. The results showed that the resistance rate of Escherichia coli, Salmonella, Klebsiella pneumoniae and Acinetobacter Baumannii to florfenicol was 34%, 4%, 10% and 16%, respectively. And all the isolated bacteria showed multiple resistance.Homology analysis revealed that floR was highly homologous among different bacteria. These results indicated that the florfenicol resistant gene floR existed widely among different bacteria and could be transmitted horizontally between different bacteria. The study lays a foundation for the wide spread of florfenicol resistant gene floR and provides a basis for clinical rational drug use.
To illuminate the florfenicol resistance of bacteria from swine and genetic characteristics of floR gene in Ningde, Fujian, 50 piglet feces samples were collected from 6 pig farms. 17 strains of florfenicol resistant Escherichia coli, 2 strains of Salmonella, 5 strains of Klebsiella pneumoniae and 8 strains of Acinetobacter baumannii were isolated. And the homology of floR gene was analyzed. The results showed that the resistance rate of Escherichia coli, Salmonella, Klebsiella pneumoniae and Acinetobacter Baumannii to florfenicol was 34%, 4%, 10% and 16%, respectively. And all the isolated bacteria showed multiple resistance.Homology analysis revealed that floR was highly homologous among different bacteria. These results indicated that the florfenicol resistant gene floR existed widely among different bacteria and could be transmitted horizontally between different bacteria. The study lays a foundation for the wide spread of florfenicol resistant gene floR and provides a basis for clinical rational drug use.
引文
[1]萧志梅.氟苯尼考的临床应用及市场状况[J].中国兽药杂志,2000(5):53-54.
[2] Ma J Y, Jiao K H. florfenicol application on swine disease[J].Shanghai Journal of Animal Husbandry and Veterinary Medicine,2005(4):13-14.
[3]黄凯,陈素娟,黄骏,等.动物源性沙门氏菌的耐药性分析及氟苯尼考类耐药基因的鉴定[J].中国畜牧兽医,2015,42(2):459-466.
[4] Vallenet D, Nordmann P, Barbe V, et al. Comparative analysis ofacinetobacters:three genomes for three lifestyles[J].PLoS One,2008,3(3):1805.
[5] Michael G B, Kadlec K, Sweeney M T, et al. ICEPmu1, anintegrative conjugative element(ICE)of Pasteurella multocida:analysis of the regions that comprise 12 antimicrobial resistancegenes[J].J Antimicrob Chemother,2012,67(1):84-90.
[6] Kadlec K, Kehrenberg C, Schwarz S. Efflux-Mediated resistance toflorfenicol and/or chloramphenicol in Bordetella bronchiseptica:identification of a novel chloramphenicol exporter[J].J AntimicrobChemother,2007,59(2):191-196.
[7] Gordon L, Cloeckaert A, Doublet B, et al. Complete sequence ofthe floR-Carrying multiresistance plasmid pAB5S9 from freshwaterAeromonas bestiarum[J].J Antimicrob Chemother,2008,62(1):65-71.
[8]朱琳琦,陈鑫,罗舒心,等.肺炎克雷伯菌中氟苯尼考抗性基因及其耐药机制的研究[J].世界最新医学信息文摘,2017,17(5):20.
[9] Cloeckaert A, Baucheron S, Flaujac G, et al. Plasmid-mediatedflorfenicol resistance encoded by the floR Gene in Escherichia coliisolated from cattle[J].Antimicrob Agents Chemother,2000,44(10):2858-2860.
[10] He T, Shen J, Schwarz S, et al. Characterization of a genomic islandin Stenotrophomonas maltophilia that carries a novel floR Genevariant[J].J Antimicrob Chemother,2015,70(4):1031-1036.
[11] Blickwede M. Molecular analysis of florfenicol resistantEscherichia coli isolates from pigs[J].Antimicrob Chemother,2004,53:58-64.
[12] Clinical and Laboratory Standards Institute(2006).Performancestandards for antimicrobial susceptibility testing. Sixteenthinternational Ed. document, supplement M100-S16. CLSI:Wayne,PA.
[13]郭树源,李代军,张红娜,等.猪源耐氟苯尼考大肠杆菌的耐药性及floR基因的序列分析[J].兽医导刊,2015(9):70-78.
[14]张广群,王春光,张国磊,等.55株猪源大肠杆菌的耐药性监测[J].中国动物检疫,2006(2):34-35.
[15] Fu H L, Li Y, Wang T, et al. A Joint Susceptibility Test forImipenem and Ceftriaxone[J].China Animal Health Inspection,2012,10:62-64.
[16] Zheng Z Z, Liu J X, Li H, et al. Study and Analysis on florfenicolResistance Gene floR in Escherichia coli from Poultry and Swine[J].Acta Agriculture Boreali-sinica,2010,05:72-75.
[17] Doublet B, Schwarz S, Kehrenberg C. Florfenicol resistance genefloR is part of a novel transposon[J].Antimicrob Agents Chemother,2005,49(5):2106-2108.
[18]张平,彭琳瑶,徐昌文,等.猪源肠球菌中氟苯尼考耐药性调查及fexA基因环境研究[J].中国畜牧兽医,2018,45(6):1700-1707.
[19]李先强,赵丽,刘俊峰,等.氟苯尼考对三种猪呼吸道病原菌标准株的体外抗菌类型研究[J].新疆农业科学,2017,55(3):557-563.
[20]余波,杨莉,姜玲玲,等.猪源大肠杆菌氟苯尼考耐药基因SYBRGreen I荧光定量PCR检测试剂盒的研制[J].河南农业科学,2018,47(4):152-156.
[2] Ma J Y, Jiao K H. florfenicol application on swine disease[J].Shanghai Journal of Animal Husbandry and Veterinary Medicine,2005(4):13-14.
[3]黄凯,陈素娟,黄骏,等.动物源性沙门氏菌的耐药性分析及氟苯尼考类耐药基因的鉴定[J].中国畜牧兽医,2015,42(2):459-466.
[4] Vallenet D, Nordmann P, Barbe V, et al. Comparative analysis ofacinetobacters:three genomes for three lifestyles[J].PLoS One,2008,3(3):1805.
[5] Michael G B, Kadlec K, Sweeney M T, et al. ICEPmu1, anintegrative conjugative element(ICE)of Pasteurella multocida:analysis of the regions that comprise 12 antimicrobial resistancegenes[J].J Antimicrob Chemother,2012,67(1):84-90.
[6] Kadlec K, Kehrenberg C, Schwarz S. Efflux-Mediated resistance toflorfenicol and/or chloramphenicol in Bordetella bronchiseptica:identification of a novel chloramphenicol exporter[J].J AntimicrobChemother,2007,59(2):191-196.
[7] Gordon L, Cloeckaert A, Doublet B, et al. Complete sequence ofthe floR-Carrying multiresistance plasmid pAB5S9 from freshwaterAeromonas bestiarum[J].J Antimicrob Chemother,2008,62(1):65-71.
[8]朱琳琦,陈鑫,罗舒心,等.肺炎克雷伯菌中氟苯尼考抗性基因及其耐药机制的研究[J].世界最新医学信息文摘,2017,17(5):20.
[9] Cloeckaert A, Baucheron S, Flaujac G, et al. Plasmid-mediatedflorfenicol resistance encoded by the floR Gene in Escherichia coliisolated from cattle[J].Antimicrob Agents Chemother,2000,44(10):2858-2860.
[10] He T, Shen J, Schwarz S, et al. Characterization of a genomic islandin Stenotrophomonas maltophilia that carries a novel floR Genevariant[J].J Antimicrob Chemother,2015,70(4):1031-1036.
[11] Blickwede M. Molecular analysis of florfenicol resistantEscherichia coli isolates from pigs[J].Antimicrob Chemother,2004,53:58-64.
[12] Clinical and Laboratory Standards Institute(2006).Performancestandards for antimicrobial susceptibility testing. Sixteenthinternational Ed. document, supplement M100-S16. CLSI:Wayne,PA.
[13]郭树源,李代军,张红娜,等.猪源耐氟苯尼考大肠杆菌的耐药性及floR基因的序列分析[J].兽医导刊,2015(9):70-78.
[14]张广群,王春光,张国磊,等.55株猪源大肠杆菌的耐药性监测[J].中国动物检疫,2006(2):34-35.
[15] Fu H L, Li Y, Wang T, et al. A Joint Susceptibility Test forImipenem and Ceftriaxone[J].China Animal Health Inspection,2012,10:62-64.
[16] Zheng Z Z, Liu J X, Li H, et al. Study and Analysis on florfenicolResistance Gene floR in Escherichia coli from Poultry and Swine[J].Acta Agriculture Boreali-sinica,2010,05:72-75.
[17] Doublet B, Schwarz S, Kehrenberg C. Florfenicol resistance genefloR is part of a novel transposon[J].Antimicrob Agents Chemother,2005,49(5):2106-2108.
[18]张平,彭琳瑶,徐昌文,等.猪源肠球菌中氟苯尼考耐药性调查及fexA基因环境研究[J].中国畜牧兽医,2018,45(6):1700-1707.
[19]李先强,赵丽,刘俊峰,等.氟苯尼考对三种猪呼吸道病原菌标准株的体外抗菌类型研究[J].新疆农业科学,2017,55(3):557-563.
[20]余波,杨莉,姜玲玲,等.猪源大肠杆菌氟苯尼考耐药基因SYBRGreen I荧光定量PCR检测试剂盒的研制[J].河南农业科学,2018,47(4):152-156.