光敏色素B在调控水稻苗期株高和生物量中的作用
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摘要
水稻光敏色素基因家族包括3个成员:PHYA、PHYB和PHYC。已有研究表明,phyB在调节水稻幼苗去黄化、叶角、花期以及非生物胁迫反应中具有重要作用,但是phyB对水稻苗期株高和生物量的影响仍未有报道。本研究比较了野生型和phyB突变体(phyB1和phyB2)在不同培养条件下苗期的株高、鲜重和干重,结果表明,在营养液和基质培养条件下,phyB突变体的株高均显著低于野生型,鲜重和干重与野生型差异并不明显,据此推测,phyB调控水稻苗期株高,但对生物量的影响不明显。利用基因芯片技术,比较了野生型和phyB突变体苗期地上部分的基因表达图谱,筛选到受phyB上调的基因115个,受phyB下调的基因95个;差异基因的GO富集分析结果表明,富集的基因主要参与水稻生长等生物学功能,因此推测通过调控这类基因的表达而影响苗期生长。本研究首次揭示了phyB对水稻苗期株高和生物量的影响,并进一步讨论了phyB在选育机插秧水稻品种中的潜在应用价值。
The phytochrome gene family in rice consists of three members, that is PHYA, PHYB and PHYC. Previous studies had revealed that phyB played an important role in regulating de-etiolation, leaf angle, flowering date and abiotic stress responses. However, the effects of phyB on plant height and biomass of rice seedlings had not been reported. In this study, the plant height, fresh weight and dry weight between wild type(WT) and phyB mutant(phyB1 and phyB2) seedlings grown under different culture conditions were compared. The results revealed that the plant height of phyB mutants were significantly lower than that of WT under the conditions of nutrient solution and matrix culture. However, there was no significant difference in fresh weight and dry weight between phyB mutants and WT. It was deduced that phyB regulated seedling height but had no obvious effect on biomass. The gene expression profiles of above-ground parts between WT and phyB mutants at seedling stage were compared using gene chip technology. One hundred and fifteen of differentially expressed genes(DEGs) were up-regulated by phyB, and 95 of DEGs were down-regulated by phyB. GO term enrichment analysis of DEGs revealed that the enriched genes were mainly involved in rice growth and other biological functions, it was speculated that phyB affected seedling growth by regulating the expression of these genes. This study, for the first time, revealed the effect of phyB on plant height and biomass of rice seedlings. We also discussed the potential application value of phyB in breeding mechanically-transplanted rice varieties.
The phytochrome gene family in rice consists of three members, that is PHYA, PHYB and PHYC. Previous studies had revealed that phyB played an important role in regulating de-etiolation, leaf angle, flowering date and abiotic stress responses. However, the effects of phyB on plant height and biomass of rice seedlings had not been reported. In this study, the plant height, fresh weight and dry weight between wild type(WT) and phyB mutant(phyB1 and phyB2) seedlings grown under different culture conditions were compared. The results revealed that the plant height of phyB mutants were significantly lower than that of WT under the conditions of nutrient solution and matrix culture. However, there was no significant difference in fresh weight and dry weight between phyB mutants and WT. It was deduced that phyB regulated seedling height but had no obvious effect on biomass. The gene expression profiles of above-ground parts between WT and phyB mutants at seedling stage were compared using gene chip technology. One hundred and fifteen of differentially expressed genes(DEGs) were up-regulated by phyB, and 95 of DEGs were down-regulated by phyB. GO term enrichment analysis of DEGs revealed that the enriched genes were mainly involved in rice growth and other biological functions, it was speculated that phyB affected seedling growth by regulating the expression of these genes. This study, for the first time, revealed the effect of phyB on plant height and biomass of rice seedlings. We also discussed the potential application value of phyB in breeding mechanically-transplanted rice varieties.
引文
[1] Franklin K A,Quai P H.Phytochrome functions in Arabidopsis development[J].J.Exp.Bot.,2010,61(1):11-24.
[2] 顾建伟,刘婧,薛彦久,等.光敏色素在水稻生长发育中的作用[J].中国水稻科学,2011,25(2):130-135.
[3] Kay S A,Keith B,Shinozaki K,et al.The sequence of the rice phytochrome gene[J].Nucleic Acids Res.,1989,17:2865-2866.
[4] Dehesh K,Tepperman J,Christensen A H,et al.phyB is evolutionarily conserved and constitutively expressed in rice seedling shoots[J].Mol.Gen.Genet.,1991,225:305-313.
[5] Takano M,Inagaki N,Xie X,et al.Distinct and cooperative functions of phytochromes A,B,and C in the control of deetiolation and flowering in rice[J].Plant Cell,2005,17:3311-3325.
[6] Liu J,Zhang F,Zhou J,et al.Phytochrome B control of total leaf area and stomatal density affects drought tolerance in rice[J].Plant Mol.Biol.,2012,78(3):289-300.
[7] He Y,Li Y,Cui L,et al.Phytochrome B negatively affects cold tolerance by regulating OsDREB1 gene expression through phytochrome interacting factor-like protein OsPIL16 in rice[J].Front.Plant Sci.,2016,7:1963.
[8] Jumtee K,Okazawa A,Harada K,et al.Comprehensive metabolite profiling of phyAphyBphyC triple mutants to reveal their associated metabolic phenotype in rice leaves[J].J.Biosci.Bioeng.,2009,108:151-159.
[9] Yang D,Seaton D D,Krahmer J,et al.Photoreceptor effects on plant biomass,resource allocation,and metabolic state[J].Proc.Natl.Acad.Sci.U.S.A.,2016,113:7667-7672.
[10] Krahmer J,Ganpudi A,Abbas A,et al.Phytochrome,carbon sensing,metabolism,and plant growth plasticity[J].Plant Physiol.,2018,176(2):1039-1048.
[11] Sulpice R,Pyl E T,Ishihara H,et al.Starch as a major integrator in the regulation of plant growth[J].Proc.Natl.Acad.Sci.U.S.A.,2009,106:10348-10353.
[12] Takano M,Inagaki N,Xie X,et al.Phytochromes are the sole photoreceptors for perceiving red/far-red light in rice[J].Proc.Natl.Acad.Sci.U.S.A.,2009,106:14705-14710.
[13] Zhao J,Zhou J,Wang Y,et al.Positive regulation of phytochrome B on chlorophyll biosynthesis and chloroplast development in rice[J].Rice Sci.,2013,20(4):243-248.
[2] 顾建伟,刘婧,薛彦久,等.光敏色素在水稻生长发育中的作用[J].中国水稻科学,2011,25(2):130-135.
[3] Kay S A,Keith B,Shinozaki K,et al.The sequence of the rice phytochrome gene[J].Nucleic Acids Res.,1989,17:2865-2866.
[4] Dehesh K,Tepperman J,Christensen A H,et al.phyB is evolutionarily conserved and constitutively expressed in rice seedling shoots[J].Mol.Gen.Genet.,1991,225:305-313.
[5] Takano M,Inagaki N,Xie X,et al.Distinct and cooperative functions of phytochromes A,B,and C in the control of deetiolation and flowering in rice[J].Plant Cell,2005,17:3311-3325.
[6] Liu J,Zhang F,Zhou J,et al.Phytochrome B control of total leaf area and stomatal density affects drought tolerance in rice[J].Plant Mol.Biol.,2012,78(3):289-300.
[7] He Y,Li Y,Cui L,et al.Phytochrome B negatively affects cold tolerance by regulating OsDREB1 gene expression through phytochrome interacting factor-like protein OsPIL16 in rice[J].Front.Plant Sci.,2016,7:1963.
[8] Jumtee K,Okazawa A,Harada K,et al.Comprehensive metabolite profiling of phyAphyBphyC triple mutants to reveal their associated metabolic phenotype in rice leaves[J].J.Biosci.Bioeng.,2009,108:151-159.
[9] Yang D,Seaton D D,Krahmer J,et al.Photoreceptor effects on plant biomass,resource allocation,and metabolic state[J].Proc.Natl.Acad.Sci.U.S.A.,2016,113:7667-7672.
[10] Krahmer J,Ganpudi A,Abbas A,et al.Phytochrome,carbon sensing,metabolism,and plant growth plasticity[J].Plant Physiol.,2018,176(2):1039-1048.
[11] Sulpice R,Pyl E T,Ishihara H,et al.Starch as a major integrator in the regulation of plant growth[J].Proc.Natl.Acad.Sci.U.S.A.,2009,106:10348-10353.
[12] Takano M,Inagaki N,Xie X,et al.Phytochromes are the sole photoreceptors for perceiving red/far-red light in rice[J].Proc.Natl.Acad.Sci.U.S.A.,2009,106:14705-14710.
[13] Zhao J,Zhou J,Wang Y,et al.Positive regulation of phytochrome B on chlorophyll biosynthesis and chloroplast development in rice[J].Rice Sci.,2013,20(4):243-248.