马蔺苗期耐镉性分析及鉴定指标筛选
|
摘要
为探讨马蔺苗期耐镉特性,筛选耐镉性快速鉴定指标并建立耐镉性数学评价模型,本研究通过盆栽砂培试验,以16份马蔺种质材料为试验材料,设置5个镉(Cd)浓度处理(0、50、100、200、300mg·kg-1),胁迫40d后测定马蔺种质材料的8项形态和生理指标,即株高(PH)、叶绿素(Chl)含量、地上干重(DWS)、根系干重(DWR)、叶片超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、丙二醛(MDA)含量及可溶性蛋白(SP)含量,以各单项指标的耐性指数作为衡量耐镉性的依据,运用主成分分析、隶属函数法分析和逐步回归分析等方法进行综合评价及分类。结果表明,主成分分析将镉胁迫处理下马蔺苗期的8项形态和生理指标转换成3个彼此独立的综合指标;利用隶属函数法对3个综合指标分析,得到不同种质苗期耐镉性综合评价值(D值);通过对D值聚类分析,将16份马蔺种质材料分为3个耐镉群体,其中强耐镉材料6份,中等耐镉材料7份,弱耐镉材料3份;进一步运用逐步回归法建立耐镉性预测回归模型:D=-1.414+1.076DWS+0.744SP+0.266SOD,筛选出显著影响马蔺苗期耐镉能力的3个单项指标(DWS、SOD、SP);ML018、ML019等6份强耐镉材料具有较强的吸收、忍耐和转运能力,可作为Cd污染土壤修复的备选植物。本研究为马蔺种质资源评价及新品种选育奠定了基础。
The objective of this study was to investigate the cadmium tolerance of Iris lacteal seedlings,to screen suitable evaluation indicators of cadmium-tolerance,and to establish a mathematical evaluation model for cadmium tolerance. A sand culture experiment was conducted in a greenhouse to investigate the plant height( PH),chlorophyll content( Chl),dry weight of shoot and root( DWS and DWR),superoxide dismutase activity( SOD),peroxidase activity( POD),malondialdehyde( MDA) and soluble protein( SP) of sixteen I. lactea species which were exposed to the mass ratio of cadmium contaminated with sand( 0,50,100,200 and 300 mg·kg~(-1)) for 40 days,These cadmium tolerance indexes were evaluated and classified by principal components analysis,subordination function method and stepwise regression analysis. The results showed that the eight morphological and physiological indexes at seedlings of different I. lactea accessions under cadmium stress could be classified into three independent comprehensive components using principal components analysis. The cadmium-tolerance comprehensive evaluation value( D value) of different accessions was obtained by means of subordination function method. The sixteen accessions were divided into three cadmium-tolerant types by cluster analysis. Six of them were high cadmium-resistant type,and seven were medium cadmium-resistant type,and hree materials were weak cadmium-resistant type. A mathematical evaluation model( D =-1. 414 +1. 076 DWS + 0. 744 SP + 0. 266 SOD) for I. lactea cadmium tolerance was established by means of regression analysis.According to this model,three indexes of DWS,SP and SOD closely related to the cadmium tolerance were screened out. Six high cadmium-resistance materials, such as ML018 and ML019, were the alternative plants for phytoremediation of Cd contaminated soil because of their higher ability in absorption,tolerance and transfer of Cd. This study lays the foundation for the evaluation of germplasm accessions and the breeding new varieties of I. lacteal.
The objective of this study was to investigate the cadmium tolerance of Iris lacteal seedlings,to screen suitable evaluation indicators of cadmium-tolerance,and to establish a mathematical evaluation model for cadmium tolerance. A sand culture experiment was conducted in a greenhouse to investigate the plant height( PH),chlorophyll content( Chl),dry weight of shoot and root( DWS and DWR),superoxide dismutase activity( SOD),peroxidase activity( POD),malondialdehyde( MDA) and soluble protein( SP) of sixteen I. lactea species which were exposed to the mass ratio of cadmium contaminated with sand( 0,50,100,200 and 300 mg·kg~(-1)) for 40 days,These cadmium tolerance indexes were evaluated and classified by principal components analysis,subordination function method and stepwise regression analysis. The results showed that the eight morphological and physiological indexes at seedlings of different I. lactea accessions under cadmium stress could be classified into three independent comprehensive components using principal components analysis. The cadmium-tolerance comprehensive evaluation value( D value) of different accessions was obtained by means of subordination function method. The sixteen accessions were divided into three cadmium-tolerant types by cluster analysis. Six of them were high cadmium-resistant type,and seven were medium cadmium-resistant type,and hree materials were weak cadmium-resistant type. A mathematical evaluation model( D =-1. 414 +1. 076 DWS + 0. 744 SP + 0. 266 SOD) for I. lactea cadmium tolerance was established by means of regression analysis.According to this model,three indexes of DWS,SP and SOD closely related to the cadmium tolerance were screened out. Six high cadmium-resistance materials, such as ML018 and ML019, were the alternative plants for phytoremediation of Cd contaminated soil because of their higher ability in absorption,tolerance and transfer of Cd. This study lays the foundation for the evaluation of germplasm accessions and the breeding new varieties of I. lacteal.
引文
[1]Oksana S,Abhay K,Dariusz L,Paulina K,Kazimierz S.Heavy metal-induced oxidative damage,defense reactions,and detoxification mechanisms in plants[J].Acta Physiol Plant,2013,35(4):985-999
[2]吕严凤,李长欣,蒋容,杨占彪.油枯对镉污染土壤的钝化研究[J].核农学报,2017,31(6):1166-1172
[3]Pelfrêne A,Waterlot C,Mazzuca M,Nisse C,Bidar G,Douay F.Assessing Cd,Pb,Zn human bioaccessibility in smeltercontaminated agricultural topsoils(Northern France)[J].Environmental Geochemistry and Health,2011,33(5):477-493
[4]Chaney R L,Malik M,Li Y M,Brown S L,Brewer E P,Angle J S,Baker A J.Phytoremediation of soil metals[J].Current Opinions in Biotechnology,1997,8(3):279-284
[5]刘俊祥,魏树强,翟飞飞,李伟,周晓星,孙振元.Cd2+胁迫下多年生黑麦草的生长于生理响应[J].核农学报,2015,29(3):587-594
[6]Natasha D,Surajit B,Mrinal K M.Enhanced cadmium accumulation and tolerance in transgenic tobacco overexpressing rice metal tolerance protein gene Os MTP1 is promising for phytoremediation[J].Plant Physiology and Biochemistry,2016,105:297-309
[7]吴志能,谢苗苗,王莹莹.我国复合污染土壤修复研究进展[J].农业环境科学学报,2016,35(12):2250-2259
[8]孟林,张国芳,赵茂林.水保护坡观赏优良地被植物-马蔺[J].农业新技术,2003(3):38-39
[9]孟林,毛培春,张国芳.不同居群马蔺抗旱性评价及生理指标变化分析[J].草业学报,2009,18(5):18-24
[10]毛培春,田小霞,孟林.16份马蔺种质材料苗期耐盐性评价[J].草业科学,2013,30(1):35-43
[11]郭智,黄苏珍,原海燕.Cd胁迫对马蔺和鸢尾幼苗生长、Cd积累及微量元素吸收的影响[J].生态环境,2008,17(2):651-656
[12]原海燕,黄苏珍,郭智.4种鸢尾属植物对铅锌矿区土壤中重金属的富集特征和修复潜力[J].生态环境学报,2010,19(7):1918-1922
[13]田治国,王飞.不同品种万寿菊对镉胁迫的生长和生理响应[J].西北植物学报,2013,33(10):2057-2064
[14]籍贵苏,严永路,吕梵,杜瑞恒,候冬利,任汉英,马雪,王晓昆,刘国庆,候升林,郭艳龙,付翠轻.不同高粱种质对污染土壤中重金属吸收的研究[J].中国生态农业学报,2014,22(2):185-192
[15]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:208-218
[16]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:184-185
[17]朱向涛,金松恒,哀建国,蒋海凌,王翔.牡丹不同品种耐涝性综合评价[J].核农学报,2017,31(3):607-613
[18]辛宝宝,袁庆华,王瑜.多年生黑麦草种质材料苗期耐钴性综合评价及钴离子富集特性研究[J].草地学报,2012,20(6):1123-1132
[19]李春红,姚兴东,鞠宝韬,朱明月,王海英,张惠君,敖雪,于翠梅,谢甫绨,宋书宏.不同基因型大豆耐荫性分析及其鉴定指标的筛选[J].中国农业科学,2014,47(15):2927-2939
[20]Gupta A K,Sinha S.Phytoextraction capacity of the Chenopodium album L.growing on soil amended with tannery sludge[J].Bioresource Technology,2007,98(2):442-446
[21]Mac Farlane G R,Koller C E,Blomberg S P.Accumulation and partitioning of heavy metals in mangroves:a synthesis of field-based studies[J].Chemosphere,2007,69(9):1454-1464
[22]Solan J J,Dowdy R H,Dolan M S,Linden D H.Long-term effects of biosolids applications on heavy metal bioavailability in agriculture soils[J].Journal of Environmental Quality,1997,26(4):966-974
[23]Rout G R,Samantaray S,Das P.Differential cadmium tolerance of mung bean and rice cultivars in hydroponic culture[J].Acta Agric Scandinavica(B)-Soil Plant Science,2000,49(4):234-241
[24]Sanitàdi,Toppi L,Gabbrielli R.Response to cadmium in higher plants.Environmental and Experimental Botany,1999,41(2):105-130
[25]张锡洲,张洪江,李廷轩,余海英.水稻镉耐性差异及镉低积累种质资源的筛选[J].中国生态农业学报,2013,21(11):1434-1440
[26]贾永霞,张春梅,方继宇,李弦,张世熔,徐小逊,蒲玉琳,李婷,李云.细叶百日草对镉的生长响应及富集特征研究[J].核农学报,2015,29(8):1577-1582
[27]佘玮,揭雨成,邢虎成,鲁雁伟,黄明,康万利,王栋.苎麻耐镉品种差异及其筛选指标分析[J].作物学报,2011,37(2):348-354
[28]丁海东,万延慧,齐乃敏,朱为民,杨晓峰,邵耀椿.重金属(Cd2+、Zn2+)胁迫对番茄幼苗抗氧化酶系统的影响[J].上海农业学报,2004,20(4):79-82
[29]田小霞,孟林,毛培春,高洪文.重金属Cd、Zn对长穗偃麦草生理生化特性的影响及其积累能力研究[J].农业环境科学学报,2012,31(8):1483-1490
[30]郭天荣,陈丽萍,冯其芳,戚志伟,沈佳辉.铝、镉胁迫对空心菜生长及抗氧化特性的影响[J].核农学报,2015,29(3):571-576
[31]胡国涛,杨兴,陈小米,陆扣萍,何丽芝,叶正钱,吴晓红,王海龙.速生树种竹柳对重金属胁迫的生理响应[J].环境科学学报,2016,36(10):3869-3875
[32]Florijn P J,Van Beusichem M L.Uptake and distribution of cadmium in maize inbred lines[J].Plant Soil,1993,150:25-32
[33]吴桂容,严重玲.镉对桐花树幼苗生长及渗透调节的影响[J].生态环境,2006,15(5):1003-1008
[34]Guo Q,Meng L,Mao P C,Tian X X.An assessment of Agropyron cristatum tolerance to cadmium contaminated soil[J].Biologia Plantarum,2014,58(1):174-178
[35]时萌,王芙蓉,王棚涛.植物响应重金属镉胁迫的耐性机理研究进展[J].生命科学,2016,28(4):504-512
[2]吕严凤,李长欣,蒋容,杨占彪.油枯对镉污染土壤的钝化研究[J].核农学报,2017,31(6):1166-1172
[3]Pelfrêne A,Waterlot C,Mazzuca M,Nisse C,Bidar G,Douay F.Assessing Cd,Pb,Zn human bioaccessibility in smeltercontaminated agricultural topsoils(Northern France)[J].Environmental Geochemistry and Health,2011,33(5):477-493
[4]Chaney R L,Malik M,Li Y M,Brown S L,Brewer E P,Angle J S,Baker A J.Phytoremediation of soil metals[J].Current Opinions in Biotechnology,1997,8(3):279-284
[5]刘俊祥,魏树强,翟飞飞,李伟,周晓星,孙振元.Cd2+胁迫下多年生黑麦草的生长于生理响应[J].核农学报,2015,29(3):587-594
[6]Natasha D,Surajit B,Mrinal K M.Enhanced cadmium accumulation and tolerance in transgenic tobacco overexpressing rice metal tolerance protein gene Os MTP1 is promising for phytoremediation[J].Plant Physiology and Biochemistry,2016,105:297-309
[7]吴志能,谢苗苗,王莹莹.我国复合污染土壤修复研究进展[J].农业环境科学学报,2016,35(12):2250-2259
[8]孟林,张国芳,赵茂林.水保护坡观赏优良地被植物-马蔺[J].农业新技术,2003(3):38-39
[9]孟林,毛培春,张国芳.不同居群马蔺抗旱性评价及生理指标变化分析[J].草业学报,2009,18(5):18-24
[10]毛培春,田小霞,孟林.16份马蔺种质材料苗期耐盐性评价[J].草业科学,2013,30(1):35-43
[11]郭智,黄苏珍,原海燕.Cd胁迫对马蔺和鸢尾幼苗生长、Cd积累及微量元素吸收的影响[J].生态环境,2008,17(2):651-656
[12]原海燕,黄苏珍,郭智.4种鸢尾属植物对铅锌矿区土壤中重金属的富集特征和修复潜力[J].生态环境学报,2010,19(7):1918-1922
[13]田治国,王飞.不同品种万寿菊对镉胁迫的生长和生理响应[J].西北植物学报,2013,33(10):2057-2064
[14]籍贵苏,严永路,吕梵,杜瑞恒,候冬利,任汉英,马雪,王晓昆,刘国庆,候升林,郭艳龙,付翠轻.不同高粱种质对污染土壤中重金属吸收的研究[J].中国生态农业学报,2014,22(2):185-192
[15]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:208-218
[16]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:184-185
[17]朱向涛,金松恒,哀建国,蒋海凌,王翔.牡丹不同品种耐涝性综合评价[J].核农学报,2017,31(3):607-613
[18]辛宝宝,袁庆华,王瑜.多年生黑麦草种质材料苗期耐钴性综合评价及钴离子富集特性研究[J].草地学报,2012,20(6):1123-1132
[19]李春红,姚兴东,鞠宝韬,朱明月,王海英,张惠君,敖雪,于翠梅,谢甫绨,宋书宏.不同基因型大豆耐荫性分析及其鉴定指标的筛选[J].中国农业科学,2014,47(15):2927-2939
[20]Gupta A K,Sinha S.Phytoextraction capacity of the Chenopodium album L.growing on soil amended with tannery sludge[J].Bioresource Technology,2007,98(2):442-446
[21]Mac Farlane G R,Koller C E,Blomberg S P.Accumulation and partitioning of heavy metals in mangroves:a synthesis of field-based studies[J].Chemosphere,2007,69(9):1454-1464
[22]Solan J J,Dowdy R H,Dolan M S,Linden D H.Long-term effects of biosolids applications on heavy metal bioavailability in agriculture soils[J].Journal of Environmental Quality,1997,26(4):966-974
[23]Rout G R,Samantaray S,Das P.Differential cadmium tolerance of mung bean and rice cultivars in hydroponic culture[J].Acta Agric Scandinavica(B)-Soil Plant Science,2000,49(4):234-241
[24]Sanitàdi,Toppi L,Gabbrielli R.Response to cadmium in higher plants.Environmental and Experimental Botany,1999,41(2):105-130
[25]张锡洲,张洪江,李廷轩,余海英.水稻镉耐性差异及镉低积累种质资源的筛选[J].中国生态农业学报,2013,21(11):1434-1440
[26]贾永霞,张春梅,方继宇,李弦,张世熔,徐小逊,蒲玉琳,李婷,李云.细叶百日草对镉的生长响应及富集特征研究[J].核农学报,2015,29(8):1577-1582
[27]佘玮,揭雨成,邢虎成,鲁雁伟,黄明,康万利,王栋.苎麻耐镉品种差异及其筛选指标分析[J].作物学报,2011,37(2):348-354
[28]丁海东,万延慧,齐乃敏,朱为民,杨晓峰,邵耀椿.重金属(Cd2+、Zn2+)胁迫对番茄幼苗抗氧化酶系统的影响[J].上海农业学报,2004,20(4):79-82
[29]田小霞,孟林,毛培春,高洪文.重金属Cd、Zn对长穗偃麦草生理生化特性的影响及其积累能力研究[J].农业环境科学学报,2012,31(8):1483-1490
[30]郭天荣,陈丽萍,冯其芳,戚志伟,沈佳辉.铝、镉胁迫对空心菜生长及抗氧化特性的影响[J].核农学报,2015,29(3):571-576
[31]胡国涛,杨兴,陈小米,陆扣萍,何丽芝,叶正钱,吴晓红,王海龙.速生树种竹柳对重金属胁迫的生理响应[J].环境科学学报,2016,36(10):3869-3875
[32]Florijn P J,Van Beusichem M L.Uptake and distribution of cadmium in maize inbred lines[J].Plant Soil,1993,150:25-32
[33]吴桂容,严重玲.镉对桐花树幼苗生长及渗透调节的影响[J].生态环境,2006,15(5):1003-1008
[34]Guo Q,Meng L,Mao P C,Tian X X.An assessment of Agropyron cristatum tolerance to cadmium contaminated soil[J].Biologia Plantarum,2014,58(1):174-178
[35]时萌,王芙蓉,王棚涛.植物响应重金属镉胁迫的耐性机理研究进展[J].生命科学,2016,28(4):504-512