口腔癌中肿瘤出芽与VEGF-C表达及淋巴结转移的相关关系
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摘要
目的:通过检测肿瘤出芽、淋巴结转移以及血管内皮生长因子-C(VEGF-C)表达水平,分析口腔癌中肿瘤出芽与VEGF-C表达及淋巴结转移的相关关系,为临床治疗提供理论参考。方法:选取2009年1月-2013年1月4年间在我院接受诊治且资料完整63例口腔癌患者作为研究对象,观察肿瘤出芽、VEGF-C表达和淋巴结转移情况,分析相互之间的相关关系。结果:本次纳入研究的患者中,检出肿瘤出芽患者40例,所占比例为63.5%,VEGF-C表达阳性患者39例,阳性率率为61.9%,淋巴结转移患者40例,转移率为63.5%;肿瘤出芽与淋巴结转移的符合率为84.1%,肿瘤出芽与VEGF-C的表达符合率为79.4%,VEGF-C的表达与淋巴结转移发生的符合率为76.2%。肿瘤出芽与淋巴结转移呈正相关,经Spear相关分析,r=0.932,P<0.05,与VEGF-C的表达也呈正相关,经Spear相关分析,r=0.897,P<0.05。结论:肿瘤出芽与VEGF-C的表达水平和淋巴结转移均呈正相关关系,可用于预测判断口腔癌淋巴结转移情况。
Objective: To analysis the relation of tumour budding,VEGF-C expression and lymph node metastasis by detection tumour budding,lymph node metastasis and vascular endothelial growth factor C(VEGF-C) expression level in oral carcinoma,and provide reference for clinical treatment.Methods: 63 cases of oral cancer patients who accept the diagnosis and treatment in our hospital between 2009.1 and 2013.1,and all the data integrity as the research object,to observe the tumor budding,VEGF-C expression and lymph node metastasis,and analysis of the relationship between each other.Results: The patients included in the study,the positive ratio of tumor budding,VEGF-C expression and lymph node metastasis was 40(63.5%),39(61.9%) and 40(63.5%).The accuracy of tumor budding and lymph node metastasis was 84.1%,The accuracy of tumor budding and the expression of VEGF-C coincidence rate was 79.4%,The accuracy of the expression of VEGF-C with lymph node metastasis coincidence rate was 76.2%.Budding of tumor and lymph node metastasis were positively correlated,r = 0.932,and the expression of VEGF-C were positively correlated,r = 0.897.Tumour budding was positively correlated with lymph node metastasis or VEGF-C expression,r=0.932,P<0.05 or r=0.897,P<0.05.Conclusion: Tumour budding was positively correlated with lymph node metastasis and VEGF-C expression,and can be used to predict lymph node metastasis in oral carcinoma.
Objective: To analysis the relation of tumour budding,VEGF-C expression and lymph node metastasis by detection tumour budding,lymph node metastasis and vascular endothelial growth factor C(VEGF-C) expression level in oral carcinoma,and provide reference for clinical treatment.Methods: 63 cases of oral cancer patients who accept the diagnosis and treatment in our hospital between 2009.1 and 2013.1,and all the data integrity as the research object,to observe the tumor budding,VEGF-C expression and lymph node metastasis,and analysis of the relationship between each other.Results: The patients included in the study,the positive ratio of tumor budding,VEGF-C expression and lymph node metastasis was 40(63.5%),39(61.9%) and 40(63.5%).The accuracy of tumor budding and lymph node metastasis was 84.1%,The accuracy of tumor budding and the expression of VEGF-C coincidence rate was 79.4%,The accuracy of the expression of VEGF-C with lymph node metastasis coincidence rate was 76.2%.Budding of tumor and lymph node metastasis were positively correlated,r = 0.932,and the expression of VEGF-C were positively correlated,r = 0.897.Tumour budding was positively correlated with lymph node metastasis or VEGF-C expression,r=0.932,P<0.05 or r=0.897,P<0.05.Conclusion: Tumour budding was positively correlated with lymph node metastasis and VEGF-C expression,and can be used to predict lymph node metastasis in oral carcinoma.
引文
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[2]Ginsburg I,Kohen R,Shalish M,et al.The Oxidant-ScavengingAbilities in the Oral Cavity May Be Regulated by a Collaborationamong Antioxidants in Saliva,Microorganisms,Blood Cells andPolyphenols:A Chemiluminescence-Based Study[J].PLoS One,2013,8(5):e63062
[3]Kashyap B,Reddy P S,Nalini P.Reactive lesions of oral cavity:Asurvey of 100 cases in Eluru,West Godavari district[J].Contemp ClinDent,2012,3(3):294-297
[4]Hansen M J,Bertelsen M F,Christensen H,et al.Occurrence ofPasteurellaceae bacteria in the oral cavity of selected marine mammalspecies[J].J Zoo Wildl Med,2012,43(4):828-835
[5]Wolff K D,Follmann M,Nast A.The diagnosis and treatment of oralcavity cancer[J].Dtsch Arztebl Int,2012,109(48):829-835
[6]Jablonska E,Puzewska W,Grabowska Z,et al.VEGF,IL-18 and NOproduction by neutrophils and their serum levels in patients with oralcavity cancer[J].Cytokine,2005,30(3):93-99
[7]Chien M H,Liu Y F,Hsin C H,et al.Impact of VEGF-C GenePolymorphisms and Environmental Factors on Oral CancerSusceptibility in Taiwan[J].PLoS One,2013,8(4):e60283
[8]Kasuya K,Nagakawa Y,Suzuki M,et al.Combination therapy ofgemcitabine or oral S-1 with the anti-VEGF monoclonal antibodybevacizumab for pancreatic neuroendocrine carcinoma[J].Exp TherMed,2012,3(4):599-602
[9]Gandolfo M,Keszler A,Lanfranchi H,et al.Increased subepithelialvascularization and VEGF expression reveal potentially malignantchanges in human oral mucosa lesions[J].Oral Surg Oral Med OralPathol Oral Radiol Endod,2011,111(4):486-493
[10]Homma Y,Hamano T,Otsuki Y,et al.Severe tumor budding is a riskfactor for lateral lymph node metastasis in early rectal cancers[J].JSurg Oncol,2010,102(3):230-234
[11]Liu Y T,Lin L W,Chen C Y,et al.Polymorphism of angiotensinI-converting enzyme gene is related to oral cancer and lymph nodemetastasis in male betel quid chewers[J].Oral Oncol,2012,48(12):1257-1262
[12]Liu X,Kolokythas A,Wang J,et al.Gene Expression Signatures ofLymph Node Metastasis in Oral Cancer:Molecular Characteristicsand Clinical Significances[J].Curr Cancer Ther Rev,2010,6(4):294-307
[13]Oliveira-Costa J P,Oliveira L R,Da S G,et al.Topoisomeraseexpression in oral squamous cell carcinoma:relationship with cancerstem cells profiles and lymph node metastasis[J].J Oral Pathol Med,2012,41(10):762-768
[14]Oliveira D T,Biassi T P,Faustino S E,et al.Eosinophils may predictoccult lymph node metastasis in early oral cancer[J].Clin OralInvestig,2012,16(6):1523-1528
[15]Zhao Y C,Ni X J,Wang M H,et al.Tumor-derived VEGF-C,but notVEGF-D,promotes sentinel lymph node lymphangiogenesis prior tometastasis in breast cancer patients[J].Med Oncol,2012,29(4):2594-2600
[16]Wang X.VEGF-C:an indicator of lymph node metastasis for patientsthat underwent esophagectomy[J].Hepatogastroenterology,2012,59(119):2027-2031
[17]Tanaka T,Ishiguro H,Kuwabara Y,et al.Vascular endothelial grow-th factor C(VEGF-C)in esophageal cancer correlates with lymphnode metastasis and poor patient prognosis[J].J Exp Clin Cancer Res,2010,29:83
[18]薛敏,于江波,张月,等.TGF-β_1在应力介导的牙周膜成纤维细胞增殖中的作用及其机制[J].现代生物医学进展,2013,13(7):1240-1243+1344Xue Min,Yu Jiang-bo,Zhang Yue,et al.The Effect of TGF-β1 onProliferation by Cultured Periodontal Ligament Fibroblasts underStress[J].Progress in Modern Biomedicine,2013,13(7):1240-1243+1344
[19]Masuda R,Kijima H,Imamura N,et al.Tumor budding is asignificant indicator of a poor prognosis in lung squamous cellcarcinoma patients[J].Mol Med Rep,2012,6(5):937-943
[20]Kai K,Kohya N,Kitahara K,et al.Tumor budding anddedifferentiation in gallbladder carcinoma:potential for theprognostic factors in T2 lesions[J].Virchows Arch,2011,459(4):449-456
[21]李栋,聂代邦,王海军,等.Fat-1基因真核表达载体的构建及其对人口腔鳞癌细胞脂肪酸含量的影响[J].现代生物医学进展,2011,11(11):2117-2121Li Dong,Nie Dai-bang,Wang Hai-jun,et al.Construction of Fat-1Eukaryotic Expression Vector and Its Effect on Fatty Acids Contentof Human Oral Squamous Cell Carcinoma Cells[J].Progress inModern Biomedicine,2011,11(11):2117-2121
[22]Sert B S,Inan M G,Ciris I M,et al.Tumor budding in colorectalcarcinomas[J].Turk Patoloji Derg,2012,28(1):61-66