多壁碳纳米管修饰碳糊电极测定爽肤水中甲硝唑
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摘要
研究甲硝唑在多壁碳纳米管修饰碳糊电极上的电化学行为,建立多壁碳纳米管修饰碳糊电极测定爽肤水中甲硝唑的方法。在pH 1.84的BR缓冲溶液中,以经过1 mg/mL多壁碳纳米管修饰的碳糊电极为工作电极,采用循环伏安法扫描试样溶液,记录扫描图谱的峰电位和峰电流。甲硝唑于–0.4 V处有明显的还原峰,其峰电流与甲硝唑的浓度在10～500μmol/L范围内具有良好的线性关系,相关系数为0.999 2,检出限为0.132μmol/L。实际样品加标回收率为98.8%～102.4%,测定结果的相对标准偏差为1.3%～2.3%(n=5)。甲硝唑在多壁碳纳米管修饰碳糊电极上有良好的响应,电极选择性好、灵敏度高;该方法简便、经济,可应用于化妆品、药品中甲硝唑的测定。
The electrochemical behavior of metronidazole at carbon paste electrode modified by multi-walled carbon nanotubes was studied. A method for the determination of metronidazole in toner water by carbon paste electrode modi?ed with multi-walled carbon nanotubes was established. In BR buffer solution at pH 1.84, the carbon paste electrode modi?ed by 1 mg/mL multi-walled carbon nanotubes was used as working electrode. The sample solution was scanned by cyclic voltammetry, and the peak potential and peak current of the scanning spectrum were recorded. The peak current of metronidazole had a good linear relationship with the concentration of metronidazole in the range of 10–500 μmol/L. The correlation coef?cient was 0.999 2, and the detection limit was 0.132 μmol/L. The recoveries of the actual samples were98.8%–102.4%, and the relative standard deviations were 1.3%–2.3%(n=5). Metronidazole had a good response on carbon paste electrode modi?ed by multi-walled carbon nanotubes with good selectivity and sensitivity. The method is simple and economical, it can be applied to the determination of metronidazole in cosmetics and pharmaceuticals.
The electrochemical behavior of metronidazole at carbon paste electrode modified by multi-walled carbon nanotubes was studied. A method for the determination of metronidazole in toner water by carbon paste electrode modi?ed with multi-walled carbon nanotubes was established. In BR buffer solution at pH 1.84, the carbon paste electrode modi?ed by 1 mg/mL multi-walled carbon nanotubes was used as working electrode. The sample solution was scanned by cyclic voltammetry, and the peak potential and peak current of the scanning spectrum were recorded. The peak current of metronidazole had a good linear relationship with the concentration of metronidazole in the range of 10–500 μmol/L. The correlation coef?cient was 0.999 2, and the detection limit was 0.132 μmol/L. The recoveries of the actual samples were98.8%–102.4%, and the relative standard deviations were 1.3%–2.3%(n=5). Metronidazole had a good response on carbon paste electrode modi?ed by multi-walled carbon nanotubes with good selectivity and sensitivity. The method is simple and economical, it can be applied to the determination of metronidazole in cosmetics and pharmaceuticals.
引文
[1]刘艳梅.硝基咪唑类药物的电化学行为及其与血清白蛋白的相互作用研究[D].山西:山西大学,2008:1-4.
[2]陆军,庞燕军,李彦博,等.高效液相色谱法同时测定祛痘产品中6种抗生素及甲硝唑[J].色谱,2012,30(6):641-646.
[3]CLARE H,DELLA W M S,WONG K M,et al.Determination of dimetridazole and metronidazole in poultry and porcine tissues by gas chromatograpy-electron capture negative ionization mass spectrometry[J].Analytica chimica acta,2005,530(1):23-31.
[4]JIN W R,LI W,XU Q,et al.Quantitative assay of metronidazole by capillary zone electrophoresis with amperometric detection at a gold microelectrode[J].Electrophoresis,2000,21(7):1 409-1 414.
[5]王立琦,贺利民,曾振灵,等.液相色谱-串联质谱检测兽药残留中的基质效应研究进展[J].质谱学报,2011,32(6):321-332.
[6]刘华良,李放,杨润,等.超高效液相色谱-串联质谱法分析化妆品中的常见抗生素及甲硝唑[J].色谱,2009,27(1):50-53.
[7]马雄辉,韦柳鸽,吴婷,等.铜-金属有机框架物修饰电极的制备及甲硝唑测定[J].分析测试学报,2017,36(1):91-95.
[8]李茜.双波长紫外分光光度法测定甲硝唑滴眼液的含量[J].现代中西医结合杂志,2013,22(18):2 021-2 023.
[9]张弛,潘家荣,帅瑞琪,等.动物性食品中硝基咪唑类兽药多残留酶联免疫检测方法的建立[J].核农学报,2016,30(2):323-331.
[10]ELKADY E F,MAHROUSE M A.Reversed-phase ion-pair HPLC and TLC-densitometric methods for the simultaneous determination of ciprofloxacin hydrochloride and metronidazole in tablets[J].Chromatographia,2011,73(3-4):297-305.
[11]TAN S Z,JIANG J H,YAN B N,et al.Preparation of a novel fluorescence probe based on covalent immobilization by emulsion polymerization and its applica-tion to the determination of metronidazole[J].Analytica chimica acta,2006,560(1-2):191-196.
[12]JIANG X H,LIN X Q.Voltammetry of the interaction of metronidazole with DNA and its analytical applications[J].Bioelectrochemistry,2006,68(2):206-212.
[13]SADEGHI S,HEMMATI M,GARMROODI A.Preparation of Ag-nanoparticles/ionic-liquid modified screen-printed electrode and its application in the determination of metronidazole[J].Electroanalysis,2013,25(1):316-322.
[14]JOSEPH W.Analytical electrochemistry[M].Germany:WileyVCH,2006:30-33.
[2]陆军,庞燕军,李彦博,等.高效液相色谱法同时测定祛痘产品中6种抗生素及甲硝唑[J].色谱,2012,30(6):641-646.
[3]CLARE H,DELLA W M S,WONG K M,et al.Determination of dimetridazole and metronidazole in poultry and porcine tissues by gas chromatograpy-electron capture negative ionization mass spectrometry[J].Analytica chimica acta,2005,530(1):23-31.
[4]JIN W R,LI W,XU Q,et al.Quantitative assay of metronidazole by capillary zone electrophoresis with amperometric detection at a gold microelectrode[J].Electrophoresis,2000,21(7):1 409-1 414.
[5]王立琦,贺利民,曾振灵,等.液相色谱-串联质谱检测兽药残留中的基质效应研究进展[J].质谱学报,2011,32(6):321-332.
[6]刘华良,李放,杨润,等.超高效液相色谱-串联质谱法分析化妆品中的常见抗生素及甲硝唑[J].色谱,2009,27(1):50-53.
[7]马雄辉,韦柳鸽,吴婷,等.铜-金属有机框架物修饰电极的制备及甲硝唑测定[J].分析测试学报,2017,36(1):91-95.
[8]李茜.双波长紫外分光光度法测定甲硝唑滴眼液的含量[J].现代中西医结合杂志,2013,22(18):2 021-2 023.
[9]张弛,潘家荣,帅瑞琪,等.动物性食品中硝基咪唑类兽药多残留酶联免疫检测方法的建立[J].核农学报,2016,30(2):323-331.
[10]ELKADY E F,MAHROUSE M A.Reversed-phase ion-pair HPLC and TLC-densitometric methods for the simultaneous determination of ciprofloxacin hydrochloride and metronidazole in tablets[J].Chromatographia,2011,73(3-4):297-305.
[11]TAN S Z,JIANG J H,YAN B N,et al.Preparation of a novel fluorescence probe based on covalent immobilization by emulsion polymerization and its applica-tion to the determination of metronidazole[J].Analytica chimica acta,2006,560(1-2):191-196.
[12]JIANG X H,LIN X Q.Voltammetry of the interaction of metronidazole with DNA and its analytical applications[J].Bioelectrochemistry,2006,68(2):206-212.
[13]SADEGHI S,HEMMATI M,GARMROODI A.Preparation of Ag-nanoparticles/ionic-liquid modified screen-printed electrode and its application in the determination of metronidazole[J].Electroanalysis,2013,25(1):316-322.
[14]JOSEPH W.Analytical electrochemistry[M].Germany:WileyVCH,2006:30-33.