聚酰胺树脂纯化富集沉香叶中主要黄酮苷元的工艺
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摘要
目的利用单因素试验法优选沉香叶中4种主要的黄酮苷元成分的提取工艺。方法采用单因素实验设计,以沉香叶中主要的黄酮苷元成分芫花素、芹菜素、羟基芫花素和木犀草素为对照成分,利用高效液相色谱法测定4种主要黄酮苷元的含量。结果 60~100目聚酰胺树脂为纯化沉香叶4种主要黄酮苷元的最优聚酰胺树脂类型,最佳工艺为,上样流速2BV·h~(-1)(柱体积Bed Column,简称BV),上样液浓度0.09mg·m L~(-1),最大上样量为100BV(178.4mg),最佳洗脱剂为30%乙醇除杂,50%乙醇洗脱,洗脱体积为24BV,50%乙醇洗脱段4种黄酮苷元平均纯度为51.37%,达到中药有效部位要求,4种主要黄酮苷元成分总提取率高达84%。结论为后期合理提取纯化富集沉香叶中黄酮苷元类成分的药用资源提供了工艺参考。
OBJECTIVE To optimize extraction and purification technology of 4 main flavonoids from Aquilaria sinensis Leaves by polyamide resin. METHODS To develop a HPLC method for determination of 4 major flavonoid components,including genkwanin,apigenin,luteolin,hydroxygenkwanin. Absorption and elution behaviors of polyamide resin for the 4 flavonoids was investigated by absorption-elution test and signal factor test. RESULTS The optimized technology parameters were as follow: sample flow rate was 2 BV ·h~(-1); concentration of sample solution was0. 09 mg·m L~(-1); maximum sample volumn was 100 BV( 178. 4 mg); impurities elution was 30% ethanol,then eluted with 24 BV of 50% ethanol. The average purity of 4 flavonoids was 51. 37%. The extraction rate of 4 flavonoids was84%. CONCLUSION This study could provide a good extraction and purification medthod for Flavonoids in Aquilaria sinensis Leaves.
OBJECTIVE To optimize extraction and purification technology of 4 main flavonoids from Aquilaria sinensis Leaves by polyamide resin. METHODS To develop a HPLC method for determination of 4 major flavonoid components,including genkwanin,apigenin,luteolin,hydroxygenkwanin. Absorption and elution behaviors of polyamide resin for the 4 flavonoids was investigated by absorption-elution test and signal factor test. RESULTS The optimized technology parameters were as follow: sample flow rate was 2 BV ·h~(-1); concentration of sample solution was0. 09 mg·m L~(-1); maximum sample volumn was 100 BV( 178. 4 mg); impurities elution was 30% ethanol,then eluted with 24 BV of 50% ethanol. The average purity of 4 flavonoids was 51. 37%. The extraction rate of 4 flavonoids was84%. CONCLUSION This study could provide a good extraction and purification medthod for Flavonoids in Aquilaria sinensis Leaves.
引文
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[14]王红刚.HPLC法测定沉香叶中芫花素含量研究[J].中医药导报,2008,14(3):69-70.
[15]韩卫娟.沉香茶保护DNA氧化损伤的活性及其机制研究[D].广州中医药大学,2013.
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[2]聂春晓,宋月林,等.白木香叶化学成分的研究[J].中国中药杂志,2012,37(2):858-860.
[3]林焕泽,李红念,等.沉香叶的研究进展[J].今日药学,2011,21(9):547-549.
[4]邓幸运,周绿颖,等.沉香叶不同提取部位的体外抗氧化作用研究[J].中国药房,2016,27(16):2181-2184.
[5]段宙位,李维国,等.沉香叶黄酮类化合物的提取及其抗氧化活性[J].食品科学,2015,36(6):45-50.
[6]何艾,李维国,等.纤维素酶辅助提取沉香叶黄酮及其抗氧化活性测定[J].食品科技,2015,(5):233-237.
[7]吴秀荣,梅全喜,等.白木香叶提取物对LPS诱导RAW264.7巨噬细胞炎症因子的影响[J].今日药学,2012,227(8):471-473.
[8]黄河胜,马传庚,等.黄酮类化合物药理作用研究进展[J].中国中药杂志,2000,25(10):589-592.
[9]杨懋勋,毛双,等.白木香叶不同提取部位对NO-2的清除作用研究[J].北华大学学报(自然),2012,13(4):406-409.
[10]林芳花,彭永宏,等.沉香叶提取工艺及其抗氧化活性实验研究[J].中国野生植物资源,2011,30(4):35-37.
[11]吴秀荣,李红念,等.沉香叶与沉香药材平喘作用的对比研究[J].今日药学,2013,(6):346-347.
[12]陈地灵,吴祎,等.沉香茶提取物的体外抗氧化和体内降血脂作用评价[J].现代食品科技,2013,(6):1198-1201.
[13]王红刚,周敏华,等.沉香叶抗肿瘤活性化学成分研究[J].林产化学与工业,2008,28(2):1-5.
[14]王红刚.HPLC法测定沉香叶中芫花素含量研究[J].中医药导报,2008,14(3):69-70.
[15]韩卫娟.沉香茶保护DNA氧化损伤的活性及其机制研究[D].广州中医药大学,2013.
[16]段宇位,李维国,等.沉香叶黄酮类化合物的提取及其抗氧化活性[J].食品科学,2014,36(6):45-50.