复方苦玄参颗粒质量标准初步研究
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摘要
为建立复方苦玄参颗粒的质量标准,采用薄层色谱法(TCL)对复方苦玄参颗粒中的苦玄参、桃金娘根进行定性分析;采用高效液相色谱法(HPLC)对复方苦玄参颗粒中的苦玄参苷IA进行含量测定。TLC表明,复方苦玄参颗粒中的苦玄参、桃金娘根均与其对应的标准药材在相同的位置有一致的斑点;HPLC表明,苦玄参苷IA在21.08μg/mL~105.4μg/mL范围内线性关系良好(R2=0.999 3),平均加样回收率为98.07%,RSD=0.01%(n=6)。表明建立的定性及定量分析方法操作简便,结果准确可靠,可作为复方苦玄参颗粒中苦玄参、桃金娘根的鉴别及苦玄参苷IA的含量测定。
To establish the quality standard for Compound Kuxuanshen Granule,the thin layer chromatography(TLC)method was used for qualitative analysis of Picria felterrae and the root of Rhodomyrtus tomentosa,and high performance liquid chromatography(HPLC)method was utilized to determine the content of picfeltarraenin IA.TLC results showed that the Picria felterrae and the root of Rhodomyrtus tomentosain Compound Kuxuanshen Granule had the same spots in the same position with its corresponding standard herbs;HPLC results showed that picfeltarraenin IAhad good linear relationship within 21.08-105.4μg/mL(R2=0.999 7),the average sample recovery rate was 98.07%,RSD=0.01%(n=6).The qualitative and quantitative analysis methods mentioned above were simple,accurate and reliable,which could be used as the method of identification of Picria feltarrae and the root of Rhodomyrtus tomentosain Compound Kuxuanshen Granule and content determination of picfeltarraenin IA.
To establish the quality standard for Compound Kuxuanshen Granule,the thin layer chromatography(TLC)method was used for qualitative analysis of Picria felterrae and the root of Rhodomyrtus tomentosa,and high performance liquid chromatography(HPLC)method was utilized to determine the content of picfeltarraenin IA.TLC results showed that the Picria felterrae and the root of Rhodomyrtus tomentosain Compound Kuxuanshen Granule had the same spots in the same position with its corresponding standard herbs;HPLC results showed that picfeltarraenin IAhad good linear relationship within 21.08-105.4μg/mL(R2=0.999 7),the average sample recovery rate was 98.07%,RSD=0.01%(n=6).The qualitative and quantitative analysis methods mentioned above were simple,accurate and reliable,which could be used as the method of identification of Picria feltarrae and the root of Rhodomyrtus tomentosain Compound Kuxuanshen Granule and content determination of picfeltarraenin IA.
引文
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[4]黄燕,肖艳芬,甄汉深,等.苦玄参体外抗菌作用的实验研究[J].广西中医药,2008,31(1):46-47.
[5]Zhang Y,Wang S,Li Y,et al.Sophocarpine and matrine inhibit the production of TNF-alpha and IL-6in murine macrophages and prevent cachexia-related symptoms induced by colon26adenocarcinoma in mice[J].Int Immunopharmacol,2008,8(13-14):1767-1772.
[6]周芳,李萍,陈勇,等.苦玄参干浸膏抗炎镇痛作用的实验研究[J].中国中医药科技,2006,13(4):244-245.
[7]Kurd F,Samavati V.Water soluble polysaccharides fromSpirulina platensis:Extraction and in-vitro anti-cancer activity[J].Int J Biol Macromol,2015,74:498-506.
[8]Constantine G.Translational systems approaches to the biology of inflammation and healing[J].Immunopharmacol Immunotoxicol,2010,32(2):181.
[9]李萍,周芳,陈勇.苦玄参提取物解热作用的实验研究[J].时珍国医国药,2007,18(11):2638-2638.
[10]谢建英.桃金娘根提取物体外抗氧化活性研究[J].食品科技,2013(12):232-234.
[11]中国医学科学院药用植物资源开发研究所.中药志[M].北京:人民卫生出版社,1998.
[12]中国兽药典委员会.中华人民共和国兽药典:二部[M].2015版.北京:中国农业出版社,2015.
[13]湖南省食品药品监督管理局.湖南省中药材标准[M].湖南长沙:湖南科学技术出版社,2010.