德式乳杆菌QS306产胞外多糖的特性研究
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摘要
该文将德氏乳杆菌QS306分别在MRS培养基和脱脂乳培养基进行培养,然后进行醇沉,透析并用苯酚硫酸法测定胞外多糖的产量且进行抗氧化活性的研究。结果表明,脱脂乳培养基中胞外多糖的产量是MRS培养基中胞外多糖产量的5倍;脱脂乳培养基中所产胞外多糖对DPPH·和OH·的清除能力比MRS培养基中所产的胞外多糖的清除能力好,并且具有较强的还原力。因此在脱脂乳培养基中培养的德氏乳杆菌QS306所产的胞外多糖产量高且具有较好的抗氧化活性。
Lactobacillus delbrueckii QS306 was cultured in MRS medium and skim milk medium,followed by alcohol precipitation,dialysis and determination of the yield of exopoly saccharides by phenol sulfuric acid method and the study of antioxidant activity. The results showed that the yield of exopoly saccharides in skim milk medium was 5 times that of exopoly saccharides in MRS medium. The scavenging ability of exopoly saccharides produced in skim milk medium was better than in MRS medium for DPPH·and OH·. The exopoly saccharides produced in the MRS medium had a good scavenging ability and a strong reducing power.Therefore,the exopoly saccharides produced by Lactobacillus delbrueckii QS306 cultured in skim milk medium has a high yield and has good antioxidant activity.
Lactobacillus delbrueckii QS306 was cultured in MRS medium and skim milk medium,followed by alcohol precipitation,dialysis and determination of the yield of exopoly saccharides by phenol sulfuric acid method and the study of antioxidant activity. The results showed that the yield of exopoly saccharides in skim milk medium was 5 times that of exopoly saccharides in MRS medium. The scavenging ability of exopoly saccharides produced in skim milk medium was better than in MRS medium for DPPH·and OH·. The exopoly saccharides produced in the MRS medium had a good scavenging ability and a strong reducing power.Therefore,the exopoly saccharides produced by Lactobacillus delbrueckii QS306 cultured in skim milk medium has a high yield and has good antioxidant activity.
引文
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[2]尹胜利,杜鉴,徐晨.乳酸菌的研究现状及其应用[J].食品科技,2012(9):25-29
[3] Ahrazem O, Gómez-Miranda B, Prieto A, et al. An acidic water-soluble cell wall polysaccharide:a chemotaxonomic marker for Fusarium, and Gibberella[J]. Mycological Research, 2000, 104(5):603-610
[4]陈营,孙莉萍,曾勇,等.一株乳酸菌胞外多糖产生的影响因素及其提取[J].微生物学通报, 2005, 32(4):85-90
[5]马静,裴家伟,吴荣荣,等.影响乳酸菌胞外多糖产量的因素研究[J].中国乳品工业, 2003, 31(5):12-16
[6] Ricciardi A, Clementi F. Exopolysaccharides from lactic acid bacteria:structure, production and technological applications.[J]. Italian Journal of Food Science, 2000, 12(1):23-45
[7] Garcíaochoa F, Santos V E, Casas J A, et al. Xanthan gum:production, recovery, and properties[J]. Biotechnology Advances, 2000,18(7):549
[8]王瑞琼,张红星,熊利霞,等.乳酸菌胞外多糖分离纯化方法研究进展[J].食品科学, 2008, 29(8):700-703
[9] Guo C F, Zhang L W, Li J Y, et al. Screening of Bile Salt HydrolaseActive Lactic Acid Bacteria for Potential Cholesterol-Lowering Probiotic Use[J]. Advanced Materials Research, 2012,345:139-146
[10]陈芳,李建科, Lim Aik Leng.新型微生物多糖-结冷胶类多糖[J].广西轻工业, 2007, 23(5):1-2,12
[11]邵丽.产胞外多糖乳杆菌的筛选及其多糖的分离、结构和生物活性研究[D].无锡:江南大学, 2015
[12]李盛钰,曾宪鹏,杨贞耐.提高乳酸菌胞外多糖产量的途径[J].食品与生物技术学报, 2009, 28(3):289-293
[13]崔学敏.益生菌胞外多糖分离与生物学活性研究[D].沈阳:辽宁大学, 2016
[14]贾红倩,刘嵬,梁立,等.红曲霉菌胞外多糖的分离纯化、结构鉴定及抗氧化活性测定[J].食品工业科技, 2017, 38(12):92-96
[15]双全,张海霞,卢宇,等.野生黑果枸杞化学成分及抗氧化活性研究[J].食品工业科技, 2017, 38(4):94-100
[16]刘佳,潘道东.硒化乳酸菌胞外多糖对小鼠腹腔巨噬细胞免疫功能的影响[J].营养学报, 2013, 35(1):35-38
[17] Kanmani P, Satish k R, Yuvaraj N, et al. Production and purification of a novel exopolysaccharide from lactic acid bacterium Streptococcus phocae PI80 and its functional characteristics activity in vitro[J].Bioresource Technology, 2011, 102(7):4827-4833