医用聚乙二醇小檗碱液对大鼠腹部术后肠黏连的预防作用
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摘要
目的研究不同浓度的医用聚乙二醇小檗碱液预防大鼠腹部手术后肠黏连的作用,评价药物对预防腹部手术黏连的作用。方法选用SD雄性大鼠造腹部手术肠黏连模型,随机分为6组:假手术组、模型对照组、阳性对照粘停宁组、聚乙二醇组、0.75‰医用聚乙二醇小檗碱液组以及1.5‰医用聚乙二醇小檗碱液组。将各组溶液2 mL均匀涂于肠表面,涂抹3次,间隔5 min,逐层缝合关闭腹腔。10天后解剖动物进行腹腔液白细胞计数和黏连程度的分级。结果与模型对照组比较,阳性对照组、聚乙二醇组、0.75‰医用聚乙二醇小檗碱液组以及1.5‰医用聚乙二醇小檗碱液组均降低大鼠肠黏连程度(P<0.05)。0.75‰医用聚乙二醇小檗碱液组以及1.5‰医用聚乙二醇小檗碱液组较模型组白细胞计数显著降低。结论医用聚乙二醇小檗碱液对腹部手术后引起的肠黏连有较好的预防作用。
Objective To investigate the preventive effect of medical polyethylene glycol berberine solutions on rat postoperative abdominal adhesion.Methods Male SD rats were used to establish postoperative abdominal adhesion model.The animals were randomly divided into six groups:the sham group,positive control group(Zhan ting ning),model group,polyethylene glycol group,medical polyethylene glycol berberine solution group(0.75‰ berberine),and medical polyethylene glycol berberine solution group(1.5‰ berberine).After opening of the abdominal cavity,2 mL of certain solution were evenly embrocated to the surface of the intestine and applied three times at 5 minute intervals,then sutured the abdominal cavity.The number of leukocyte in peritoneal fluid were counted and the adhesion degree were identified 10 days later.Results Compared with the model group,the degree of adhesion were all decreased in positive control group,polyethylene glycol group,medical polyethylene glycol berberine solution group(0.75‰ berberine),and medical polyethylene glycol berberine solution group(1.5‰ berberine)(P<0.05).Compared with the model group,leukocyte number of the medical polyethylene glycol berberine solution group(0.75‰ berberine)and medical polyethylene glycol berberine solution group(1.5‰berberine)were both reduced(P<0.05).Conclusion Medical polyethylene glycol berberine solution has preventive effect on postoperative abdominal adhesion.
Objective To investigate the preventive effect of medical polyethylene glycol berberine solutions on rat postoperative abdominal adhesion.Methods Male SD rats were used to establish postoperative abdominal adhesion model.The animals were randomly divided into six groups:the sham group,positive control group(Zhan ting ning),model group,polyethylene glycol group,medical polyethylene glycol berberine solution group(0.75‰ berberine),and medical polyethylene glycol berberine solution group(1.5‰ berberine).After opening of the abdominal cavity,2 mL of certain solution were evenly embrocated to the surface of the intestine and applied three times at 5 minute intervals,then sutured the abdominal cavity.The number of leukocyte in peritoneal fluid were counted and the adhesion degree were identified 10 days later.Results Compared with the model group,the degree of adhesion were all decreased in positive control group,polyethylene glycol group,medical polyethylene glycol berberine solution group(0.75‰ berberine),and medical polyethylene glycol berberine solution group(1.5‰ berberine)(P<0.05).Compared with the model group,leukocyte number of the medical polyethylene glycol berberine solution group(0.75‰ berberine)and medical polyethylene glycol berberine solution group(1.5‰berberine)were both reduced(P<0.05).Conclusion Medical polyethylene glycol berberine solution has preventive effect on postoperative abdominal adhesion.
引文
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[11]王德娟,莫家骢,谢家伦.剖腹术后腹膜黏连发生机制和预防的研究现状及进展[J].2005,26(5):273-275.
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[14]胡明彦,陈斌,代少华.开腹手术切口与腹腔镜手术切口术后肠黏连发生率的比较[J].中华消化外科杂志,2002,1(5):330-332.
[15]曾莉.腹腔黏连研究进展[J].南京中医药大学学报,2004,20(2):127-128.
[2]Karimi M,Yazdan Asadi S,Parsaei P,et al.The effect of ethanol extract of Rose(Rosa damascena)on intra-abdominal adhesions after laparotomy in rats[J].Wounds,2016,28(5):167-174.
[3]Schindler AE.Gonadotropin-releasing hormone agonists for prevention of postoperative adhesion:an overview[J].Gynecol Endocrinol,2004,19(1):51-55.
[4]Aldemir M,Ozturk H,Erten C,et al.The preventive effect of Rofecoxib in postoperative intraperitoneal adhesions[J].Acta Cbir Belg,2004,104(1):97-100.
[5]Cetin M,Ak D,Duran B,et al.Use of methylene blue and N,Ocarboxymethylchitosan to prevent postoperative adhesions in a rat uterine horn model[J].Fertil Steril,2003,80(Suppl 2):698-701.
[6]Alatas E,Gunal O,Atalas O,et al.Octreotide prevents postoperative adhesion formation by suppressing peritoneal myeloperoxidase activity[J].Hepatogastroenterology,2000,47(34):1034-1036.
[7]Hugh TB,Ellis H.Postoperative abdominal adhesion.An experimental study of the value of polyvinylpyrrolidone in prophylaxis[J].Br J Surq,1964,51(5):381-382.
[8]蔡华芳.术后腹腔内黏连的预防研究进展[J].中国医药导刊,2008,10(8):1179-1182.
[9]Zhang Y,Li X,Zhang Q,et al.Berberine hydrochloride prevents postsurgery intestinal adhesion and inflammation in rats[J].J Pharmacol Exp Ther,2014,349(3):417-426.
[10]李英民,徐世新,佟刚.预防腹部手术后肠黏连的现状与进展[J].中国当代医药,2009,16(24):137-139.
[11]王德娟,莫家骢,谢家伦.剖腹术后腹膜黏连发生机制和预防的研究现状及进展[J].2005,26(5):273-275.
[12]李继承.腹腔黏连形成及防治的新进展[J].国外医学·外科学分册,1988(4):210-213.
[13]孙鸿斌,杨关根,李有柱,等.羧甲基纤维素预防术后肠黏连的实验研究[J].中国中西医结合外科杂志,2003,9(3):212-213.
[14]胡明彦,陈斌,代少华.开腹手术切口与腹腔镜手术切口术后肠黏连发生率的比较[J].中华消化外科杂志,2002,1(5):330-332.
[15]曾莉.腹腔黏连研究进展[J].南京中医药大学学报,2004,20(2):127-128.