聚六亚甲基双胍对蝙蝠蛾幼虫病原菌粉棒束孢的杀菌效果和毒性评价
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摘要
为了解聚六亚甲基双胍对冬虫夏草寄主昆虫蝙蝠蛾幼虫病原菌粉棒束孢的杀菌消毒效果和对蝙蝠蛾幼虫的毒性,本文采用了悬液定量杀菌试验、现场试验、稳定性试验及昆虫毒性评价方法,对该消毒剂的消毒相关性能及安全性进行试验观察。结果显示含2g/L聚六亚甲基双胍消毒液对粉棒束孢孢子悬液或者现场试验作用45–60min,杀菌率均可达到99.00%以上。聚六亚甲基双胍消毒原液及稀释液37℃保存3个月与1个月的下降率分别为2.10%和3.20%,性质比较稳定、杀菌效率短期内无显著降低;含量≤10g/L聚六亚甲基双胍消毒液对蝙蝠蛾幼虫死亡影响不显著,无明显的不良反应,属于实际无毒类物质。聚六亚甲基双胍消毒液性质稳定、杀菌效果好、无毒,可用于蝙蝠蛾幼虫饲料、饲养盒等的消毒。
Suspension quantitative germicidal test, field test, stability test and toxicity evaluation were carried out to observe the germicidal efficacy of a new disinfectant, polyhexamethylene biguandine, against Hepialus larva pathogen Isaria farinosa. The results showed that disinfection rate against Isaria farinosa spores by using 2g/L polyhexamethylene biguandine solution reached more than 99.00%and field test also obtained the same result.Polyhexamethylene biguandine concentration the solution decreased only 2.10%and 3.20%respectively when neat solution and diluted solution stored in three months and one month respectively at 37°C.The disinfection solution of 10g/L did not cause significant mortality of Hepialus larvas.In conclusion,polyhexamethylene biguandine solution was qualitatively stable with good sterilizing effect and safety and could be used as disinfectant for sterilizing fodder and feeding box of Hepialus larvas.
Suspension quantitative germicidal test, field test, stability test and toxicity evaluation were carried out to observe the germicidal efficacy of a new disinfectant, polyhexamethylene biguandine, against Hepialus larva pathogen Isaria farinosa. The results showed that disinfection rate against Isaria farinosa spores by using 2g/L polyhexamethylene biguandine solution reached more than 99.00%and field test also obtained the same result.Polyhexamethylene biguandine concentration the solution decreased only 2.10%and 3.20%respectively when neat solution and diluted solution stored in three months and one month respectively at 37°C.The disinfection solution of 10g/L did not cause significant mortality of Hepialus larvas.In conclusion,polyhexamethylene biguandine solution was qualitatively stable with good sterilizing effect and safety and could be used as disinfectant for sterilizing fodder and feeding box of Hepialus larvas.
引文
Anon.,2012.Disinfection technology practice(S).2012Chinese Edition.Ministry of Health of the People’s Republic of China,Beijing.19-23(in Chinese)
Anon.,2015.Pharmacopoeia of the People’s Republic of China.2015 Chinese Edition.Vol.1.China Medical Science Press,Beijing.115(in Chinese)
Chen QZ,Cui YJ,Li H,Qian ZY,Sun KQ,Zhang CZ,Gao L,2009.Study on the application of Polyhexamthylene biguanide compound disinfection solution in hospital.Hebei Medical Journal,31(6):668-670(in Chinese)
Cohen N,Cohen J,Asatiani MD,Varshney VK,Yu HT,Yang YC,Li YH,Mau JL,Wasser SP,2014.Chemical composition and nutritional and medicinal value of fruit bodies and submerged cultured mycelia of culinary-medicinal higher basidiomycetes mushrooms.International Journal of Medicinal Mushrooms,16(3):273-291
Cui SY,Chen L,2011.The research progress of polyheamethylene guanidine.Chinese Journal of Disinfection,28(6):749-751(in Chinese)
Cui YJ,Zhang CZ,Guo JM,Chen SL,Han YS,2008.The toxicity study of a polyheamethylene guanidine compound disinfection solution.Chinese Journal of Disinfection,25(1):85-86(in Chinese)
Dai ZY,Liu YK,Wang F,1998.Practical antibacterial medicine.Shanghai Science Technology Press,Shanghai.5-11,12-28(in Chinese)
Dong F,Li AP,Yang B,Liu WJ,Wang FM,Cui SY,Sun QH,2012.Research on the germicidal efficacy and toxicity of a new type of guanidines disinfectant.Chinese Journal of Disinfection,29(1):25-27(in Chinese)
Feng YY,2003.Paecilomyces farinosus:new counter to pest.Forestry of China,2003(12):42(in Chinese)
Jang SH,Kim SH,Lee HY,Jang SH,Jang H,Chae SW,Jung SJ,So BO,Ha KC,Sin HS,Jang YS,2015.Immune-modulating activity of extract prepared from mycelial culture of Chinese caterpillar mushroom,Ophiocordyceps sinensis(Ascomycetes).International Journal of Medicinal Mushrooms,17(12):1189-1199
Li CQ,He YL,2008.Application of biguanide as disinfectant and antiseptic.Food and Drug,10(7):44-47(in Chinese)
Li NN,Yu W,2015.Research progress of properties and application of polyhexamethylene guanidine disinfectant.Detergent&Cosmetics,38(9):36-39(in Chinese)
Liang ZQ,2015.Flora fungorum sinicorum.China Science Press,Beijing.43:110-111(in Chinese)
Liu F,Wu XL,Liu Y,Chen DX,Zhang DL,Yang DJ,2016.Progress on molecular biology of Isaria farinosa,pathogen of host of Ophiocordyceps sinensis during the artificial culture.China Journal of Chinese Materia Medica,41(3):403-409(in Chinese)
Lu LX,Zhen YY,Cai DL,Lin JM,Xu J,2011.Research on disinfection property of polyhexamethylene biguanidine hydrochloride.Chinese Journal of Disinfection,25(5):536-539
Lu ZH,Shi P,He YC,Zhang DL,He ZY,Chen SJ,Tu YQ,Li L,Li F,Zeng W,2015.Review on natural enemies and diseases in the artificial cultivation of Chinese caterpillar mushroom,Ophiocordyceps sinensis(Ascomycetes).International Journal of Medicinal Mushrooms,17(7):693-700
Lu LX,Zhen YY,Cai DL,Lin JM,Xu J,2011.Research on disinfection property of polyhexamethylene biguanidine hydrochloride.Chinese Journal of Disinfection,28(5):536-539
Nakamura K,Shinozuka K,Yoshikawa N,2015.Anticancer and antimetastatic effects of cordycepin,an active component of Cordyceps sinensis.Journal of Pharmacological Sciences,127(1):53-56
Shen HR,2014.Study on genetic diversity and biological characteristics of Paecilomyces farinosus.Master Thesis,Sichuan Agricultural University,Ya’an.1-58(in Chinese)
Tian ZM,Zhao KY,Chen L,Yang B,Sun GN,Sun WK,Cui SY,2014.Research on the disinfection effect of guanidine disinfectant to legionella.Chinese Journal of Disinfection,31(11):1155-1157,1160(in Chinese)
Zhang FE,Zhang YN,Song H,Zhang YZ,2010.Experimental observation on selection of neutralizer and germicidal efficacy of poly hexamethylene guanidine.Chinese Journal of Disinfection,27(3):266-267(in Chinese)
Zhang L,Yao GY,Teng HK,2013.The research progress of industrial water treatment with guanidine disinfectant.In:Annual symposium of large scale nitrogenous fertilizer industry,Guiyang.40:95-96(in Chinese)
Zhao Y,Song JB,2006.Evaluation of effects of disinfection for mess kits with guanidines disinfectants.Medical Journal of National Defending Forces in Southwest China,16(1):65-67(in Chinese)
Zheng FN,Ding NP,Zhou ZW,Yang CH,Zhang ZF,Wang C,2015.Research on field disinfection effect of polyheamethylene guanidine.Guangxi Journal of Light Industry,2015(10):104-105(in Chinese)
陈巧芝,崔玉杰,栗华,钱振宇,孙克勤,张春长,高蕾,2009.聚六亚甲基双胍复方消毒剂医院消毒应用研究.河北医药,31(6):668-670
崔树玉,陈璐,2011.胍类消毒剂及其研究进展.中国消毒学杂志,28(6):749-751
崔玉杰,张春长,郭金铭,陈素良,韩艳淑,2008.一种聚六亚甲基双胍复方消毒剂毒性试验观察.中国消毒学杂志,25(1):85-86
戴自英,刘裕昆,汪复,1998.实用抗菌药物学.上海:上海科学技术出版社.5-11,12-28
董非,李爱萍,杨彬,刘文杰,王凤鸣,崔树玉,孙启华,2012.一种新型胍类消毒剂杀菌效果及毒性研究.中国消毒学杂志,29(1):25-27
冯玉元,2003.粉拟青霉:害虫的新克星.中国林业,2003(12):42
国家药典委员会(编),2015.中华人民共和国药典.2015年版一部.北京:中国医药科技出版社.115
李传秋,贺艳丽,2008.双胍类消毒防腐剂的应用.食品与药品,10(7):44-47
李妮妮,于文,2015.聚六亚甲基胍类消毒剂性能及应用研究进展.日用化学品科学,38(9):36-39
梁宗琦,2015.中国真菌志(B).北京:科学出版社.43:110-111
刘飞,伍晓丽,刘莹,陈大霞,张德利,杨大坚,2016.冬虫夏草人工培殖过程中寄主昆虫粉拟青霉病原菌的分子生物学研究.中国中药杂志,41(3):403-409
申惠荣,2014.贡嘎蝙蝠蛾粉拟青霉的遗传多样性及生物学特性研究.四川农业大学硕士论文,雅安.1-58
田忠梅,赵克义,陈璐,杨彬,孙广宁,孙文魁,崔树玉,2014.一种新型胍类消毒剂对军团杆菌杀灭效果研究.中国消毒学杂志,31(11):1155-1157,1160
卫生部卫生法制与监督司,2012.消毒技术规范(S).北京:中华人民共和国卫生部.19-23
张福娥,张亚尼,宋晖,张亚增,2010.聚六亚甲基胍的中和剂选择及其杀菌效果试验观察.中国消毒学杂志,27(3):266-267
张磊,姚光源,腾厚开,2013.工业水处理用有机胍杀菌剂研究进展.第二十一届全国大型合成氨装置技术年会论文集,贵阳.40:95-96
郑飞龙,丁年平,周卓为,杨础华,张增峰,王春,2015.聚六亚甲基胍现场消毒效果的研究.轻工科技,2015(10):104-105
Anon.,2015.Pharmacopoeia of the People’s Republic of China.2015 Chinese Edition.Vol.1.China Medical Science Press,Beijing.115(in Chinese)
Chen QZ,Cui YJ,Li H,Qian ZY,Sun KQ,Zhang CZ,Gao L,2009.Study on the application of Polyhexamthylene biguanide compound disinfection solution in hospital.Hebei Medical Journal,31(6):668-670(in Chinese)
Cohen N,Cohen J,Asatiani MD,Varshney VK,Yu HT,Yang YC,Li YH,Mau JL,Wasser SP,2014.Chemical composition and nutritional and medicinal value of fruit bodies and submerged cultured mycelia of culinary-medicinal higher basidiomycetes mushrooms.International Journal of Medicinal Mushrooms,16(3):273-291
Cui SY,Chen L,2011.The research progress of polyheamethylene guanidine.Chinese Journal of Disinfection,28(6):749-751(in Chinese)
Cui YJ,Zhang CZ,Guo JM,Chen SL,Han YS,2008.The toxicity study of a polyheamethylene guanidine compound disinfection solution.Chinese Journal of Disinfection,25(1):85-86(in Chinese)
Dai ZY,Liu YK,Wang F,1998.Practical antibacterial medicine.Shanghai Science Technology Press,Shanghai.5-11,12-28(in Chinese)
Dong F,Li AP,Yang B,Liu WJ,Wang FM,Cui SY,Sun QH,2012.Research on the germicidal efficacy and toxicity of a new type of guanidines disinfectant.Chinese Journal of Disinfection,29(1):25-27(in Chinese)
Feng YY,2003.Paecilomyces farinosus:new counter to pest.Forestry of China,2003(12):42(in Chinese)
Jang SH,Kim SH,Lee HY,Jang SH,Jang H,Chae SW,Jung SJ,So BO,Ha KC,Sin HS,Jang YS,2015.Immune-modulating activity of extract prepared from mycelial culture of Chinese caterpillar mushroom,Ophiocordyceps sinensis(Ascomycetes).International Journal of Medicinal Mushrooms,17(12):1189-1199
Li CQ,He YL,2008.Application of biguanide as disinfectant and antiseptic.Food and Drug,10(7):44-47(in Chinese)
Li NN,Yu W,2015.Research progress of properties and application of polyhexamethylene guanidine disinfectant.Detergent&Cosmetics,38(9):36-39(in Chinese)
Liang ZQ,2015.Flora fungorum sinicorum.China Science Press,Beijing.43:110-111(in Chinese)
Liu F,Wu XL,Liu Y,Chen DX,Zhang DL,Yang DJ,2016.Progress on molecular biology of Isaria farinosa,pathogen of host of Ophiocordyceps sinensis during the artificial culture.China Journal of Chinese Materia Medica,41(3):403-409(in Chinese)
Lu LX,Zhen YY,Cai DL,Lin JM,Xu J,2011.Research on disinfection property of polyhexamethylene biguanidine hydrochloride.Chinese Journal of Disinfection,25(5):536-539
Lu ZH,Shi P,He YC,Zhang DL,He ZY,Chen SJ,Tu YQ,Li L,Li F,Zeng W,2015.Review on natural enemies and diseases in the artificial cultivation of Chinese caterpillar mushroom,Ophiocordyceps sinensis(Ascomycetes).International Journal of Medicinal Mushrooms,17(7):693-700
Lu LX,Zhen YY,Cai DL,Lin JM,Xu J,2011.Research on disinfection property of polyhexamethylene biguanidine hydrochloride.Chinese Journal of Disinfection,28(5):536-539
Nakamura K,Shinozuka K,Yoshikawa N,2015.Anticancer and antimetastatic effects of cordycepin,an active component of Cordyceps sinensis.Journal of Pharmacological Sciences,127(1):53-56
Shen HR,2014.Study on genetic diversity and biological characteristics of Paecilomyces farinosus.Master Thesis,Sichuan Agricultural University,Ya’an.1-58(in Chinese)
Tian ZM,Zhao KY,Chen L,Yang B,Sun GN,Sun WK,Cui SY,2014.Research on the disinfection effect of guanidine disinfectant to legionella.Chinese Journal of Disinfection,31(11):1155-1157,1160(in Chinese)
Zhang FE,Zhang YN,Song H,Zhang YZ,2010.Experimental observation on selection of neutralizer and germicidal efficacy of poly hexamethylene guanidine.Chinese Journal of Disinfection,27(3):266-267(in Chinese)
Zhang L,Yao GY,Teng HK,2013.The research progress of industrial water treatment with guanidine disinfectant.In:Annual symposium of large scale nitrogenous fertilizer industry,Guiyang.40:95-96(in Chinese)
Zhao Y,Song JB,2006.Evaluation of effects of disinfection for mess kits with guanidines disinfectants.Medical Journal of National Defending Forces in Southwest China,16(1):65-67(in Chinese)
Zheng FN,Ding NP,Zhou ZW,Yang CH,Zhang ZF,Wang C,2015.Research on field disinfection effect of polyheamethylene guanidine.Guangxi Journal of Light Industry,2015(10):104-105(in Chinese)
陈巧芝,崔玉杰,栗华,钱振宇,孙克勤,张春长,高蕾,2009.聚六亚甲基双胍复方消毒剂医院消毒应用研究.河北医药,31(6):668-670
崔树玉,陈璐,2011.胍类消毒剂及其研究进展.中国消毒学杂志,28(6):749-751
崔玉杰,张春长,郭金铭,陈素良,韩艳淑,2008.一种聚六亚甲基双胍复方消毒剂毒性试验观察.中国消毒学杂志,25(1):85-86
戴自英,刘裕昆,汪复,1998.实用抗菌药物学.上海:上海科学技术出版社.5-11,12-28
董非,李爱萍,杨彬,刘文杰,王凤鸣,崔树玉,孙启华,2012.一种新型胍类消毒剂杀菌效果及毒性研究.中国消毒学杂志,29(1):25-27
冯玉元,2003.粉拟青霉:害虫的新克星.中国林业,2003(12):42
国家药典委员会(编),2015.中华人民共和国药典.2015年版一部.北京:中国医药科技出版社.115
李传秋,贺艳丽,2008.双胍类消毒防腐剂的应用.食品与药品,10(7):44-47
李妮妮,于文,2015.聚六亚甲基胍类消毒剂性能及应用研究进展.日用化学品科学,38(9):36-39
梁宗琦,2015.中国真菌志(B).北京:科学出版社.43:110-111
刘飞,伍晓丽,刘莹,陈大霞,张德利,杨大坚,2016.冬虫夏草人工培殖过程中寄主昆虫粉拟青霉病原菌的分子生物学研究.中国中药杂志,41(3):403-409
申惠荣,2014.贡嘎蝙蝠蛾粉拟青霉的遗传多样性及生物学特性研究.四川农业大学硕士论文,雅安.1-58
田忠梅,赵克义,陈璐,杨彬,孙广宁,孙文魁,崔树玉,2014.一种新型胍类消毒剂对军团杆菌杀灭效果研究.中国消毒学杂志,31(11):1155-1157,1160
卫生部卫生法制与监督司,2012.消毒技术规范(S).北京:中华人民共和国卫生部.19-23
张福娥,张亚尼,宋晖,张亚增,2010.聚六亚甲基胍的中和剂选择及其杀菌效果试验观察.中国消毒学杂志,27(3):266-267
张磊,姚光源,腾厚开,2013.工业水处理用有机胍杀菌剂研究进展.第二十一届全国大型合成氨装置技术年会论文集,贵阳.40:95-96
郑飞龙,丁年平,周卓为,杨础华,张增峰,王春,2015.聚六亚甲基胍现场消毒效果的研究.轻工科技,2015(10):104-105