聚六亚甲基双胍盐酸盐与氟环唑对水稻稻曲病菌的联合毒力测定
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摘要
本研究采用菌丝生长速率法测定了聚六亚甲基双胍盐酸盐(polyhexamethylene biguanidine hydrochloride)与氟环唑(epoxiconazole)混配对水稻稻曲病菌(Ustilaginoidea virens)的毒力,旨在明确二者混配对水稻稻曲病菌的联合作用类型及最佳配比。结果表明,聚六亚甲基双胍盐酸盐、氟环唑对水稻稻曲病菌的EC_(50)分别为44.111、1.743 mg·L~(-1),氟环唑对水稻稻曲病菌的室内毒力活性明显高于聚六亚甲基双胍盐酸盐。聚六亚甲基双胍盐酸盐与氟环唑25:1配比表现为相加作用;5:1、10:1、15:1和20:1四个配比表现为增效作用,其中,配比为10:1和15:1的SR分别为1.64和1.66,增效作用明显。
This study was conducted to clarify the co-effects of mixed polyhexamethylene biguanidine hydrochloride( PHMB) and epoxiconazole on Ustilaginoidea virens,and to find out their best mixed ratio by mycelium growth rate method. The results showed that the EC_(50) of PHMB and epoxiconazole to U. virens were 44. 111 and 1. 743 mg·L~(-1),respectively,which indicated that the indoor toxicity of epoxiconazole to U. virens was more active compared with PHMB. In the 5 different mixing ratios of PHMB and epoxiconazole,the ratio of 25:1 could do additively,and the ratio of 5:1,10 :1,15 :1 and 20 :1 had synergistic effect on U. virens; among which,the ratio of 10:1 and 15:1 showed the strongest synergistic effect with the synergistic coefficients as 1. 64 and 1. 66,respectively.
This study was conducted to clarify the co-effects of mixed polyhexamethylene biguanidine hydrochloride( PHMB) and epoxiconazole on Ustilaginoidea virens,and to find out their best mixed ratio by mycelium growth rate method. The results showed that the EC_(50) of PHMB and epoxiconazole to U. virens were 44. 111 and 1. 743 mg·L~(-1),respectively,which indicated that the indoor toxicity of epoxiconazole to U. virens was more active compared with PHMB. In the 5 different mixing ratios of PHMB and epoxiconazole,the ratio of 25:1 could do additively,and the ratio of 5:1,10 :1,15 :1 and 20 :1 had synergistic effect on U. virens; among which,the ratio of 10:1 and 15:1 showed the strongest synergistic effect with the synergistic coefficients as 1. 64 and 1. 66,respectively.
引文
[1]季宏平,张匀华,王芉,等.水稻稻曲病发生为害调查及药效防治研究[J].中国农学通报,2001,17(4):37-38.
[2]陈德林,刘赵康.水稻稻曲病的发生规律及药剂防治技术[J].内蒙古农业科技,2005(7):246-248.
[3]Nakamura K,Izumiyama N,Ohtsubo K,et al.Apoptosis induced in the liver,kidney and urinary bladder of mice by the fungal toxin produced by Ustilaginoidea virens[J].Mycotoxins,1993,38:25-30.
[4]白元俊,将宝山.水稻稻曲病菌的毒素研究[J].辽宁农业科学,1997(1):30-33.
[5]Zhou Y L,Pan Y J,Xie X W,et al.Genetic diversity of rice false smut fungus,Ustilaginoidea virens and its pronounced differentiation of populations in north China[J].Journal of Phytopathol.,2008,156(9):559-564.
[6]王大为,蔡继新.稻曲病发病程度与水稻株型的相关性[J].垦殖与稻作,2004(3):24-25.
[7]何海永,陈小均,詹金碧,等.稻曲病发生与氮肥施用的关系初探[J].安徽农业科学,2008,36(20):8665-8666.
[8]李友荣,唐善军,高杜娟,等.籼型杂交晚稻新组合穗部性状与稻曲病抗性相关性研究[J].中国农学通报,2011,27(21):234-238.
[9]甘林,林延邦,阮宏椿,等.抗感稻曲病水稻品种的生殖期性状分析[J].中国农学通报,2013,29(36):343-346.
[10]胡东维,王疏.稻曲病菌侵染机制现状与展望[J].中国农业科学,2012,45(22):4604-4611.
[11]黄磊,胡建坤,俞咪娜,等.稻曲病菌致病力丧失突变菌株B-1015的T-DNA标记基因的克隆[J].中国农业科学,2014,47(13):2552-2562.
[12]阮宏椿,杨秀娟,石妞妞,等.不同杀菌剂对水稻稻曲病菌的室内毒力及田间药效[J].福建农业学报,2013,28(6):580-583.
[13]李竞生,张敏,陈华保,等.几种杀菌剂对稻曲病菌的室内毒力测定[J].安徽农业科学,2009,37(21):10042-10044.
[14]陈莉,许娟,丁克坚,等.药剂对稻曲病菌的毒力测定及复配研究[J].植物保护,2012,38(3):159-161.
[15]耿忠义,赵京岚,任勇攀,等.氰烯菌酯与己唑醇及其混配对小麦赤霉病菌的影响[J].农学学报,2011(10):29-32.
[16]宗兆锋,康振生.植物病理学原理[M].北京:中国农业出版社,2002:244-245.
[17]黄彰欣,植物化学保护实验指导[M].北京:中国农业出版社,2007:56-58.
[18]农业部农药检定所.NY/T 1156.2—2006农药室内生物测定试验准则杀菌剂第2部分:抑制病原真菌菌丝生长试验平皿法[S].北京:中国农业出版社,2006:1-2.
[19]East G C,Mclntyre J E,Shao J.Polybiguanides:synthesis and characterization of polybiguanides containing hexamethylene groups[J].Polymer,1997,38(15):3973-3984.
[20]Hu Y,Du Y,Yang J,et al.Synthesis,characterization and antibacterial activity of guanidinyl ated chitosan[J].Carbohydrate Polymers,2007,67(1):66-72.
[21]Francois I E J A,Cammue B P A,Borgers M,et al.Azoles:mode of antifungal action and resistance development.Effect of miconazole on endogenous reactive oxygen species production in Candida albicans[J].Anti-Infective Agents in Medicinal Chemistry,2006,5(1):3-13.
[22]王遂,陶艳玲,陈丹峰,等.胍类消毒剂的制备、性能与应用[J].广东化学,2009,36(9):58.
[23]张先菊,苏裕心,任哲,等.聚六亚甲基双胍盐酸盐的杀菌活性及现场空气消毒效果观察[J].中国消毒学杂志,2013,30(11):1016-1021.
[2]陈德林,刘赵康.水稻稻曲病的发生规律及药剂防治技术[J].内蒙古农业科技,2005(7):246-248.
[3]Nakamura K,Izumiyama N,Ohtsubo K,et al.Apoptosis induced in the liver,kidney and urinary bladder of mice by the fungal toxin produced by Ustilaginoidea virens[J].Mycotoxins,1993,38:25-30.
[4]白元俊,将宝山.水稻稻曲病菌的毒素研究[J].辽宁农业科学,1997(1):30-33.
[5]Zhou Y L,Pan Y J,Xie X W,et al.Genetic diversity of rice false smut fungus,Ustilaginoidea virens and its pronounced differentiation of populations in north China[J].Journal of Phytopathol.,2008,156(9):559-564.
[6]王大为,蔡继新.稻曲病发病程度与水稻株型的相关性[J].垦殖与稻作,2004(3):24-25.
[7]何海永,陈小均,詹金碧,等.稻曲病发生与氮肥施用的关系初探[J].安徽农业科学,2008,36(20):8665-8666.
[8]李友荣,唐善军,高杜娟,等.籼型杂交晚稻新组合穗部性状与稻曲病抗性相关性研究[J].中国农学通报,2011,27(21):234-238.
[9]甘林,林延邦,阮宏椿,等.抗感稻曲病水稻品种的生殖期性状分析[J].中国农学通报,2013,29(36):343-346.
[10]胡东维,王疏.稻曲病菌侵染机制现状与展望[J].中国农业科学,2012,45(22):4604-4611.
[11]黄磊,胡建坤,俞咪娜,等.稻曲病菌致病力丧失突变菌株B-1015的T-DNA标记基因的克隆[J].中国农业科学,2014,47(13):2552-2562.
[12]阮宏椿,杨秀娟,石妞妞,等.不同杀菌剂对水稻稻曲病菌的室内毒力及田间药效[J].福建农业学报,2013,28(6):580-583.
[13]李竞生,张敏,陈华保,等.几种杀菌剂对稻曲病菌的室内毒力测定[J].安徽农业科学,2009,37(21):10042-10044.
[14]陈莉,许娟,丁克坚,等.药剂对稻曲病菌的毒力测定及复配研究[J].植物保护,2012,38(3):159-161.
[15]耿忠义,赵京岚,任勇攀,等.氰烯菌酯与己唑醇及其混配对小麦赤霉病菌的影响[J].农学学报,2011(10):29-32.
[16]宗兆锋,康振生.植物病理学原理[M].北京:中国农业出版社,2002:244-245.
[17]黄彰欣,植物化学保护实验指导[M].北京:中国农业出版社,2007:56-58.
[18]农业部农药检定所.NY/T 1156.2—2006农药室内生物测定试验准则杀菌剂第2部分:抑制病原真菌菌丝生长试验平皿法[S].北京:中国农业出版社,2006:1-2.
[19]East G C,Mclntyre J E,Shao J.Polybiguanides:synthesis and characterization of polybiguanides containing hexamethylene groups[J].Polymer,1997,38(15):3973-3984.
[20]Hu Y,Du Y,Yang J,et al.Synthesis,characterization and antibacterial activity of guanidinyl ated chitosan[J].Carbohydrate Polymers,2007,67(1):66-72.
[21]Francois I E J A,Cammue B P A,Borgers M,et al.Azoles:mode of antifungal action and resistance development.Effect of miconazole on endogenous reactive oxygen species production in Candida albicans[J].Anti-Infective Agents in Medicinal Chemistry,2006,5(1):3-13.
[22]王遂,陶艳玲,陈丹峰,等.胍类消毒剂的制备、性能与应用[J].广东化学,2009,36(9):58.
[23]张先菊,苏裕心,任哲,等.聚六亚甲基双胍盐酸盐的杀菌活性及现场空气消毒效果观察[J].中国消毒学杂志,2013,30(11):1016-1021.