苄索氯铵与聚六亚甲基双胍协同杀菌效果的评价
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摘要
以大肠杆菌和金黄色葡萄球菌为实验菌种,通过比较苄索氯铵与聚六亚甲基双胍(PHMB)两种杀菌剂单独使用和混合使用的杀菌效果,考察了其杀菌率的高低。结果表明,这两种杀菌剂单独使用时对上述两种常见的细菌具有很好的杀菌效果,并且两种杀菌剂具有协同杀菌作用,当苄索氯铵与PHMB以1∶1的比例混合使用时,可达最佳协同作用。
In this paper, E. coli and S. aureus were used as experimental strains. The bactericidal effects of bacteriocin and polyhexamethylene biguanide( PHMB) as fungicides used alone and in combination were examined and their bactericidal rates were examined. The results showed that the two fungicides have good bactericidal effect on the above two common bacteria when used alone,and the two fungicides have a synergistic bactericidal effect. When the benzethonium chloride and PHMB are mixed in a ratio of 1∶ 1,The best synergetic effect can be achieved.
In this paper, E. coli and S. aureus were used as experimental strains. The bactericidal effects of bacteriocin and polyhexamethylene biguanide( PHMB) as fungicides used alone and in combination were examined and their bactericidal rates were examined. The results showed that the two fungicides have good bactericidal effect on the above two common bacteria when used alone,and the two fungicides have a synergistic bactericidal effect. When the benzethonium chloride and PHMB are mixed in a ratio of 1∶ 1,The best synergetic effect can be achieved.
引文
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[2]王冰姝,陈惠珍,钟昱文,等.聚六亚甲基胍类消毒剂的杀菌效果观察[J].中国热带医学,2008,8(3):491-493.
[3]赵玉萍.含氯消毒剂使用不当对护士伤害的报道[J].中国消毒学杂志,2004,21(1):72-73.
[4]许发发,陈黔,李绕梅,等.吸入高浓度含氯消毒剂致呼吸道痉挛伴休克1例[J].人民军医,2015,58(10):1248.
[5]姜允申.当心杀菌湿巾致敏[J].家庭医学,2014,(11):37.
[6]丁宏,张鹏祥,高晓譞,等. HPLC法测定化妆品中防腐剂劳拉氯铵、苄索氯铵和西他氯铵的含量[J].中国药事,2012,26(8):830-833.
[7]中华人民共和国卫生部.化妆品卫生规范[S].卫监督发[2007]1号,2007.
[8]张先菊,苏裕心,任哲,等.聚六亚甲基双胍盐酸盐的杀菌活性及现场空气消毒效果观察[J].中国消毒学杂志,2013,30(11):1016-1018.
[9]张晓玲,孙晔,董晓峰.盐酸聚六亚甲基双胍消毒剂杀菌性能的试验观察[J].中国消毒学杂志,2011,28(2):139-140.
[10]陈璐,赵克义,袁青春,等.一种即时净手液性能的试验观察[J].中国消毒学杂志,2004,21(3):208-210.
[11]邓佳伟,郝玉梅,韩泽民,等.一种消毒湿巾对口腔修复器材表面的消毒效果观察[J].中国消毒学杂志,2016,33(10):967-969.
[12]崔玉杰,张春长,陈素良,等.四种复方聚六亚甲基双胍消毒剂对白色念珠菌杀灭效果比较[J].中国消毒学杂志,2008,25(4):386-388.
[13]崔玉杰,王茜,孙克勤,等.聚六亚甲基双胍与双癸基二甲基氯化铵协同作用研究[J].医学理论与实践,2009,22(3):360-361.
[14] GILBERT P,PEMBERTON D,WILKINSON D E. Synergism within polyhexamethylene biguanide biocide formulations[J].Journal of Applied Bacteriology,2010,69(4):593-598.
[15] GARDINI F,LANCIOTTI R,SINIGAGLIA M,et al. A head space gas chromatographic approach for the monitoring of the microbial cell activity and the cell viability evaluation[J]. Journal of Microbiological Methods,1997,29(2):103-114.
[16] GU J J,HU H C,CHAI X S,et al. A new method for the determination of biological oxygen demand in domestic wastewater by headspace gas chromatography[J]. Journal of Chromatography A,2013,1308(6):32-36.
[17] CHAI X S,LUO Q,ZHU J Y. Analysis of nonvolatile species in a complex matrix by headspace gas chromatography[J]. Journal of Chromatography A,2001,909(2):249-257.