温和灸对肌筋膜激痛点TGF-β1/Smad4表达的影响
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摘要
目的:探讨温和灸扳机点干预对局部组织TGF-β1和Smad4表达影响。方法:将54只SD大鼠随机分为A组(空白对照)、B组(模型对照)和C组(模型干预)3组,每组18只;每组又均分3小组(A0-A2、B0-B2和C0-C2)。B、C两组建立肌筋膜痛扳机点模型,并分别给予C1组和C2组温和灸干预。分批处死大鼠,扳机点部取材,制片并进行HE和免疫组化染色,光镜下观察其病理变化。RT-PCR对TGF-β1mRNA及Smad4m-RNA表达水平进行定量分析。结果:TGF-β1、Smad4在正常组织中弱表达,B组和C组强表达;C0组与B0组间TGF-β1mRNA、Smad4mRNA表达量比较无显著差异(P>0.05),C1组与B1组间比较差异有显著意义(P<0.01),C1组较B1组显著下降;C2组与C1组间比较差异有显著意义(P<0.01),C2组较C1组显著下降。结论:温和灸法干预能够下调TGF-β1和Smad4的表达,可能间接调节TGF-β1/Smads信号转导通路的靶基因表达,从而改善扳机点局部肌组织的病理化状态。
Objective:To investigate the effect of mild moxibustion intervention on local tissue TGF-β1 and Smad4 expression in myofascial trigger points(MTrPs). Method:Fifity-four SD rats were randomly divided into Group A(blank control), Group B(model control) and Group C(model intervention), 18 in each group, and each group was equally divided into 3 groups(A0-A2, B0-B2 and C0-C2). B and C groups set up the MTrPs model, and the C1 group and C2 group were given mild moxibustion intervention. The rats were executed before intervention, intervention for 7 and 15 days. The partial drawing, fixing and making slices. Pathological changes were observed under light microscope after HE and immunohistochmical staining. The expression levels of TGF-β1 mRNA and Smad4 mRNA were quantitatively analyzed by RT-PCR. Results: TGF-β1 and Smad4 were weakly expressed in normal tissues, and strongly expressed in B group and Group C. There was no significant difference in TGF-β1 mRNA and Smad4 m-RNAexpressions between C0 Group and B0 Group(P>0.05). There was significant difference between C1 Group and B1 Group(P<0.01), and C1 group's decreased significantly compared with that of B1 group. There was significant difference between C2 Group and C1 Group(P<0.01), and C2 group's decreased significantly compared with that of C1 group. The C2 group's was significantly lower than that of the C1 group. Conclusion:The mild moxibustion can cut down the expressions of TGF-β1 and Smad4, and the target gene expression of TGF-β1/Smads signal transduction pathway maybe indirectly regulated to improve the pathological state of the local muscle tissue in MTrPs.
Objective:To investigate the effect of mild moxibustion intervention on local tissue TGF-β1 and Smad4 expression in myofascial trigger points(MTrPs). Method:Fifity-four SD rats were randomly divided into Group A(blank control), Group B(model control) and Group C(model intervention), 18 in each group, and each group was equally divided into 3 groups(A0-A2, B0-B2 and C0-C2). B and C groups set up the MTrPs model, and the C1 group and C2 group were given mild moxibustion intervention. The rats were executed before intervention, intervention for 7 and 15 days. The partial drawing, fixing and making slices. Pathological changes were observed under light microscope after HE and immunohistochmical staining. The expression levels of TGF-β1 mRNA and Smad4 mRNA were quantitatively analyzed by RT-PCR. Results: TGF-β1 and Smad4 were weakly expressed in normal tissues, and strongly expressed in B group and Group C. There was no significant difference in TGF-β1 mRNA and Smad4 m-RNAexpressions between C0 Group and B0 Group(P>0.05). There was significant difference between C1 Group and B1 Group(P<0.01), and C1 group's decreased significantly compared with that of B1 group. There was significant difference between C2 Group and C1 Group(P<0.01), and C2 group's decreased significantly compared with that of C1 group. The C2 group's was significantly lower than that of the C1 group. Conclusion:The mild moxibustion can cut down the expressions of TGF-β1 and Smad4, and the target gene expression of TGF-β1/Smads signal transduction pathway maybe indirectly regulated to improve the pathological state of the local muscle tissue in MTrPs.
引文
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[2] Junbeom K, Hyoung Seop K, Won Hyuk C,et al. Characteristics of Myofascial pain syndrome of the Infraspinatus Muscle[J].Annals of Rehabitation Medicine,2017,4(41):573-581.
[3] L?we B,Spitzer RL,Williams JB,et al.Depression, anxiety and somatization in primary care: syndrome overlap and functional impairment[J].Gen Hosp Psychiatry,2008,30(3):191-199.
[4] Prashanth P. The Possible of Meditation in Myofascial pain syndrome:A New Hypothsis[J]. Indian journal of palliative care,2017,2(23):180-187.
[5] 苏甦,李晓玲.期口服非甾体抗炎药患者的胃肠道和心血管不良反应防治策略[J].临床药物治疗杂志,2017,15(2):7-11.
[6] 王列,马帅,赵悦,等.温和灸激痛点治疗肩背部肌筋膜疼痛综合征:随机对照研究[J].针刺研究,2016,41(4):334-337.
[7] Huang QM, Lv JJ, Ruanshi, et al.Spontaneous electrical activities at myofascial trigger points at different stages of recovery from injury in a rat model[J].Acupunture in medicine,2015,33(4):319-324.
[8] 刘琳,黄强民,汤莉.肌筋膜疼痛触发点[J].中国组织工程研究,2014,18(46):7520-7527.
[9] Bengtsson A,Henriksson K,Larsson J.Muscle biopsy in fibromyalgia:Light microscopical and histochemical findings[J].Scand J Rheumatol,1986,15(1):1-6.
[10] Wiafe B, Adesida A, Churchil T, et al.Hypoxia-increased expression of genes involved in inflammation, dedifferentiation, pro-fibrosis, and extracellular matrix remodeling of human bladder smooth muscle cells[J]. In Vitro Cell Dev Biol Anim, 2017,53(1):58-66.
[11] 滑振,吕晓东,庞立健,等.中医药防治肺纤维化相关文献的数据挖掘[J].中华中医药学刊,2017,35(10):2604-2607.
[12] 徐飞,崔文强,刘宝君,等.基于现代文献特发性肺纤维化中医证候特征研究[J].中国中医基础医学杂志,2017,23(1):87-89.
[13] 肖小花,李戎,梁繁荣,等.论中医“肺痿”一名与“肺纤维化”最为相侔[J].辽宁中医杂志,2012,39(6):1045-1047.
[14] 庞立健,王琳琳.肺纤维化(肺痿)中医病机初探[J].中华中医药学刊,2011,29(7):1596-1597.
[15] Li Y,Sun YX, Zhang CL, et al. Moxibustion Alleviates Injury inRat Focal Segmental Glome-rulerosis Model[J].Evidence-based complentary and alternative medicine,2017(11):1-11.
[16] Cheng L, Li R, Zhou M, et al.Moxibusition has a positive effect on pulmonary fibrsis: an alternative approach[J].Afr J Tradit Complement Altern Med,2017,14(2):125-129.
[17] Saito H, Tsujitani S, Oka S, et al. The expression of transforming growth factorbeta1 is significantly correlated with the expression of vascular endothelial growth factor and poor prongosis of patients with advanced gastric carcinoma[J]. Cancer,1999,86(8):1455-1462.
[18] 王养民,鲁守琳,赵志强,等.TGF-β1、Smad4在慢性非细菌性前列腺炎大鼠模型前列腺组织中的表达及意义[J].中华男科学杂志,2010,16(6):490-494.
[19] AhamedJ, Laurence J.Role of Platelet-Derived Transforming Growth Factor-β1and Reactive Oxygen Species in Radiation-Induced Origan Fibrosis[J].Antioxid Redox Sinal,2017,27(13):977-988.
[20] 张旭,谢敏,高艳,等.低氧对大鼠肺组织结构的影响及其机制的探讨[J].四川大学学报(医学版),2012,43(1):1-5.
[21] Attisano L, Lee-Hoeflich ST.TheSmads[J].GenomeBiol,2001,2(8):Revieus30101.
[22] 陈始秋,陈疾忤,陈世益,等.ShRNA介导的Smad4基因沉默对C2C12成肌细胞纤维化的影响[J].中国运动医学杂志,2011,30(12):1100-1105.