沙子岭猪背最长肌肌苷酸含量与相关基因表达的关联性分析
|
摘要
以沙子岭猪、大约克、巴克夏猪及其杂交组合巴×沙、大×巴沙猪为研究对象,测定背最长肌中肌苷酸含量,对ADSL、GPAT和PURH基因在不同群体背最长肌中的表达水平进行检测,分析其与肌苷酸含量的相关性。结果表明,沙子岭猪肌苷酸含量显著高于巴克夏和大×巴沙猪(P<0.05),其余各群体之间差异均不显著(P>0.05)。肌苷酸含量与肌肉pH1h、肉色L*值和a*值、肌内脂肪含量、剪切力等肉质指标具有显著相关性。ADSL、GPAT和PURH基因在不同群体中的表达模式基本相同,即三种基因均在沙子岭猪和巴×沙猪中高表达。ADSL基因表达量与肌苷酸含量呈极显著正相关(P<0.01),GPAT和PURH基因表达量与肌苷酸含量均呈显著正相关(P<0.05)。结果提示,ADSL、GPAT和PURH基因与肌苷酸沉积紧密相关,或可作为猪肉质风味的选择依据,为今后选育工作提供理论基础。
In this research,Shaziling,Yorkshire and Berkshire,and their crossbreeds Berkshire×Shaziling,Yorkshire×Berkshire×Shaziling pigs is chosen as the study objects.The inosine monophosphate(IMP)content in longissimus dorsi muscle was measured by HPLC.Meanwhile,the relative expressions of ADSL,GPATand PURH genes in different groups were detected by real-time PCR,and the correlations between expressions of genes and IMP content were analyzed.The results demonstrate that the IMP content in Shaziling pigs is significantly higher than that in Berkshire and Yorkshire×(Berkshire×Shaziling)pigs(P<0.05),and no significant difference is observed among other groups(P>0.05).Further research reveals that the IMP content has a significant correlation with pH1 h,meat color L*and a*,intramuscular fat content,and shear force.In addition,ADSL,GPATand PURH genes,which exhibited the similar expression patterns in different groups,show a higher expression in Shaziling and Berkshire×Shaziling pigs,respectively.Furthermore,extremely significant positive correlation between ADSLexpression and IMP content is observed(P<0.01),and there is significantly positive correlation between expression of GPAT,PURHand IMP content(P<0.05),respectively.These results suggest that ADSL,GPATand PURH genes are closely related to the process of IMP deposition,and these genes may serve as a basis for selection of pork flavor,which will provide theoretical foundation for future breeding.
In this research,Shaziling,Yorkshire and Berkshire,and their crossbreeds Berkshire×Shaziling,Yorkshire×Berkshire×Shaziling pigs is chosen as the study objects.The inosine monophosphate(IMP)content in longissimus dorsi muscle was measured by HPLC.Meanwhile,the relative expressions of ADSL,GPATand PURH genes in different groups were detected by real-time PCR,and the correlations between expressions of genes and IMP content were analyzed.The results demonstrate that the IMP content in Shaziling pigs is significantly higher than that in Berkshire and Yorkshire×(Berkshire×Shaziling)pigs(P<0.05),and no significant difference is observed among other groups(P>0.05).Further research reveals that the IMP content has a significant correlation with pH1 h,meat color L*and a*,intramuscular fat content,and shear force.In addition,ADSL,GPATand PURH genes,which exhibited the similar expression patterns in different groups,show a higher expression in Shaziling and Berkshire×Shaziling pigs,respectively.Furthermore,extremely significant positive correlation between ADSLexpression and IMP content is observed(P<0.01),and there is significantly positive correlation between expression of GPAT,PURHand IMP content(P<0.05),respectively.These results suggest that ADSL,GPATand PURH genes are closely related to the process of IMP deposition,and these genes may serve as a basis for selection of pork flavor,which will provide theoretical foundation for future breeding.
引文
[1]沈晓玲.猪肉肌苷酸沉积规律及其与新鲜度的相关性研究[D].四川雅安:四川农业大学,2008.
[2]郭辽朴.荣昌乳猪与PIC乳猪不同部位肌肉理化特性及主体风味物质研究[D].重庆:西南大学,2008.
[3]郭建凤,呼红梅,武英,等.长白猪、大约克及杜洛克肌肉品质研究[J].家畜生态学报,2009,30(1):34-39.
[4] FUJIMURA S.Identification of taste-active components in the meat of the Japanese native chicken,Hinai-dori and broilers,and the effects of feeding treatments on taste-active components[J].Anim Food Sci,1998,50(2):90-158.
[5]陈继兰,文杰,王述柏,等.鸡肉肌苷酸和肌内脂肪沉积规律研究[J].畜牧兽医学报,2005,36(8):843-845.
[6]朱吉,杨仕柳,彭英林.不同杂交组合猪的肉质测定[J].养猪,2005(5):47-49.
[7]朱吉,杨仕柳,孙建帮,等.部分湖南地方猪的肉质分析[J].养猪,2007(5):65-67.
[8]朱荣生,呼红梅,韩红,等.莱芜猪肌肉肌苷酸和肌内脂肪含量研究[J].家畜生态学报,2008,29(6):63-65.
[9]呼红梅,郭建凤,朱荣生,等.不同品种猪背最长肌肌苷酸和肌内脂肪含量的比较[J].扬州大学学报(农业与生命科学版),2011,32(3):34-36.
[10]陶勇,任善茂,郭韦敏,等.苏姜猪与其母本姜曲海猪肌肉品质的比较分析[J].黑龙江畜牧兽医,2017(20):106-107.
[11]顾庆南,司熊元,张莉,等.圩猪与杜大长三元杂交猪生长性能与肉品质的比较[J].西北农林科技大学学报(自然科学版),2016,44(6):9-15.
[12]宋倩倩,张金枝,刘健,等.不同品种猪背最长肌风味物质的研究[J].中国畜牧杂志,2016,52(19):9-12.
[13] ZHANG X D,ZHANG S J,DING Y Y,et al.Association between ADSL,GARS-AIRS-GART,DGAT1,and DECR1expression levels and pork meat quality traits[J].Genet Mol Res,2015,14(4):14 823-14 830.
[14]刘家忠,陈润生.猪肉中肌苷酸含量及其在冷藏和加热过程中变化规律的研究[J].养猪,1991(3):10-12.
[15]陶勇,刘宗华,张牧,等.不同组合猪肌肉肌苷酸含量的分析[J].黑龙江畜牧兽医,2001(10):17.
[16]李玉莲,吴买生,王建伟,等.大约克、杜洛克和巴克夏猪肌肉品质比较分析[J].养猪,2017(5):61-64.
[17]张善文,李朝晖,向拥军,等.湘沙猪配套系母系母本、父母代猪及商品猪肉质特性测定[J].饲料与畜牧,2017(18):42-46.
[18] YE M H,CHEN J L,ZHAO G P,et al.Correlation between polymorphism in ADSLand GARS-AIRS-GARTgenes with inosine 5'-monophosphate(IMP)contents in Beijing-you chickens[J].Br Poult Sci,2010,51(5):609-613.
[19]李庆海,范京辉,楼立峰,等.饲粮能量差异对猪ADSL基因mRNA表达的影响[J].养猪,2013(6):57-59.
[20]张淑静,刘力源,殷宗俊,等.不同品种猪背最长肌肌苷酸含量及相关基因表达的比较[J].食品与发酵工业,2014,40(6):187-192.
[21]徐善金,虞德兵,汪峰,等.鸭腺苷琥珀酸裂解酶基因序列特征及表达与肌肉肌苷酸含量的相关性分析[J].中国农业科学,2012,45(4):774-785.
[2]郭辽朴.荣昌乳猪与PIC乳猪不同部位肌肉理化特性及主体风味物质研究[D].重庆:西南大学,2008.
[3]郭建凤,呼红梅,武英,等.长白猪、大约克及杜洛克肌肉品质研究[J].家畜生态学报,2009,30(1):34-39.
[4] FUJIMURA S.Identification of taste-active components in the meat of the Japanese native chicken,Hinai-dori and broilers,and the effects of feeding treatments on taste-active components[J].Anim Food Sci,1998,50(2):90-158.
[5]陈继兰,文杰,王述柏,等.鸡肉肌苷酸和肌内脂肪沉积规律研究[J].畜牧兽医学报,2005,36(8):843-845.
[6]朱吉,杨仕柳,彭英林.不同杂交组合猪的肉质测定[J].养猪,2005(5):47-49.
[7]朱吉,杨仕柳,孙建帮,等.部分湖南地方猪的肉质分析[J].养猪,2007(5):65-67.
[8]朱荣生,呼红梅,韩红,等.莱芜猪肌肉肌苷酸和肌内脂肪含量研究[J].家畜生态学报,2008,29(6):63-65.
[9]呼红梅,郭建凤,朱荣生,等.不同品种猪背最长肌肌苷酸和肌内脂肪含量的比较[J].扬州大学学报(农业与生命科学版),2011,32(3):34-36.
[10]陶勇,任善茂,郭韦敏,等.苏姜猪与其母本姜曲海猪肌肉品质的比较分析[J].黑龙江畜牧兽医,2017(20):106-107.
[11]顾庆南,司熊元,张莉,等.圩猪与杜大长三元杂交猪生长性能与肉品质的比较[J].西北农林科技大学学报(自然科学版),2016,44(6):9-15.
[12]宋倩倩,张金枝,刘健,等.不同品种猪背最长肌风味物质的研究[J].中国畜牧杂志,2016,52(19):9-12.
[13] ZHANG X D,ZHANG S J,DING Y Y,et al.Association between ADSL,GARS-AIRS-GART,DGAT1,and DECR1expression levels and pork meat quality traits[J].Genet Mol Res,2015,14(4):14 823-14 830.
[14]刘家忠,陈润生.猪肉中肌苷酸含量及其在冷藏和加热过程中变化规律的研究[J].养猪,1991(3):10-12.
[15]陶勇,刘宗华,张牧,等.不同组合猪肌肉肌苷酸含量的分析[J].黑龙江畜牧兽医,2001(10):17.
[16]李玉莲,吴买生,王建伟,等.大约克、杜洛克和巴克夏猪肌肉品质比较分析[J].养猪,2017(5):61-64.
[17]张善文,李朝晖,向拥军,等.湘沙猪配套系母系母本、父母代猪及商品猪肉质特性测定[J].饲料与畜牧,2017(18):42-46.
[18] YE M H,CHEN J L,ZHAO G P,et al.Correlation between polymorphism in ADSLand GARS-AIRS-GARTgenes with inosine 5'-monophosphate(IMP)contents in Beijing-you chickens[J].Br Poult Sci,2010,51(5):609-613.
[19]李庆海,范京辉,楼立峰,等.饲粮能量差异对猪ADSL基因mRNA表达的影响[J].养猪,2013(6):57-59.
[20]张淑静,刘力源,殷宗俊,等.不同品种猪背最长肌肌苷酸含量及相关基因表达的比较[J].食品与发酵工业,2014,40(6):187-192.
[21]徐善金,虞德兵,汪峰,等.鸭腺苷琥珀酸裂解酶基因序列特征及表达与肌肉肌苷酸含量的相关性分析[J].中国农业科学,2012,45(4):774-785.