摘要
A novel phosphoprotein separation material was developed, which is constructed by a magnetic mesoporous Fe_3 O_4@TiO_2(Fe_3 O_4@mTiO_2) microsphere and a 5-aminoisophthalic acid(AIPA) monolayer that provides additional binding sites toward phosphate groups. The results of characteristic experiments demonstrated that Fe_3 O_4@mTiO_2-AIPA had good dispersability, high magnetic susceptibility, and satisfactory grafting ratio of AIPA, ascribed to the large specific surface area of the inorganic substrate. Taking advantages of these features, Fe_3 O_4@mTiO_2-AIPA was successfully utilized to separate α-casein(a typical phosphoprotein) and bovine serum albumin(BSA, a typical non-phosphoprotein) from their mixtures(molar ratio = 1:2). Through adjusting pH and polarity of solutions, the BSA and α-casein were respectively enriched in washing fraction and elution fraction. This result displays the good potential of Fe_3 O_4@mTiO_2-AIPA for application in phosphoprotein enrichment.
A novel phosphoprotein separation material was developed, which is constructed by a magnetic mesoporous Fe_3 O_4@TiO_2(Fe_3 O_4@mTiO_2) microsphere and a 5-aminoisophthalic acid(AIPA) monolayer that provides additional binding sites toward phosphate groups. The results of characteristic experiments demonstrated that Fe_3 O_4@mTiO_2-AIPA had good dispersability, high magnetic susceptibility, and satisfactory grafting ratio of AIPA, ascribed to the large specific surface area of the inorganic substrate. Taking advantages of these features, Fe_3 O_4@mTiO_2-AIPA was successfully utilized to separate α-casein(a typical phosphoprotein) and bovine serum albumin(BSA, a typical non-phosphoprotein) from their mixtures(molar ratio = 1:2). Through adjusting pH and polarity of solutions, the BSA and α-casein were respectively enriched in washing fraction and elution fraction. This result displays the good potential of Fe_3 O_4@mTiO_2-AIPA for application in phosphoprotein enrichment.
引文
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