硫氧还蛋白互作蛋白与胰岛功能及胰岛素抵抗的相关性
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摘要
目的探讨血清硫氧还蛋白互作蛋白(TXNIP)水平与胰岛功能及胰岛素抵抗(IR)的相关性。方法选取初诊断2型糖尿病(T2DM)肥胖患者(BMI≥25 kg/m2) 30例为T2DM肥胖组,T2DM非肥胖患者(BMI<25 kg/m2) 30例为T2DM非肥胖组;以同期接受体检的健康者30例为对照组,收集3组对象的一般资料,采集空腹血液样本,检测空腹血糖(FPG)、空腹胰岛素(FINS)、糖化血红蛋白(Hb A1C)、血脂指标(HDL-C,LDL-C,TG,TC),ELISA法检测血清TXNIP水平,收集数据并进行统计分析。结果 (1)T2DM肥胖组、T2DM非肥胖组、对照组血清TXNIP依次降低(P<0. 05);(2)Pearson及Spearman相关分析显示血清TXNIP与HOMA-IR、BMI、FPG、Hb A1C、FINS、TG、TC呈正相关(P<0. 05),血清TXNIP与ISI、HOMA-β水平呈负相关(P<0. 05);(3)线性逐步回归分析显示ISI、BMI为血清TXNIP的独立影响因素。结论 T2DM患者血清TXNIP的升高可能参与了胰岛素抵抗及胰岛细胞凋亡的发生。
Objective To investigate the relationship between serum thioredoxin interacting protein( TXNIP) concentration and islet function and insulin resistance( IR).Methods 30 obese patients with newly diagnosed T2 DM( BMI<25 kg/m2) were selected as group A,30 non-obese patients with T2 DM( BMI<25 kg/m2) as group B,and 30 healthy people receiving physical examination at the same time as control group C.The general data of the three groups were collected,and the blood samples were collected after 8 hours' fasting. FPG,FINS,HbA1 C and blood lipid were detected. Serum TXNIP level was detected by ELISA.Statistical methods were used to study data correlation.Results(1)The serum TXNIP levels in A,B and C groups decreased in turn( P<0. 05);(2)Pearson and Spearman correlation analysis showed that serum TXNIP levels were positively correlated with HOMA-IR,BMI,FPG,Hb A1 C,FINS,TG and TC( P<0. 05),serum TXNIP levels were negatively correlated with ISI and HOMA-β( P<0. 05);(3)Stepwise regression analysis showed that TXNIP level was independently correlated with ISI and BMI( P < 0. 05).Conclusion The elevation of serum TXNIP in T2 DM patients may be involved in insulin resistance and islet cell apoptosis.
Objective To investigate the relationship between serum thioredoxin interacting protein( TXNIP) concentration and islet function and insulin resistance( IR).Methods 30 obese patients with newly diagnosed T2 DM( BMI<25 kg/m2) were selected as group A,30 non-obese patients with T2 DM( BMI<25 kg/m2) as group B,and 30 healthy people receiving physical examination at the same time as control group C.The general data of the three groups were collected,and the blood samples were collected after 8 hours' fasting. FPG,FINS,HbA1 C and blood lipid were detected. Serum TXNIP level was detected by ELISA.Statistical methods were used to study data correlation.Results(1)The serum TXNIP levels in A,B and C groups decreased in turn( P<0. 05);(2)Pearson and Spearman correlation analysis showed that serum TXNIP levels were positively correlated with HOMA-IR,BMI,FPG,Hb A1 C,FINS,TG and TC( P<0. 05),serum TXNIP levels were negatively correlated with ISI and HOMA-β( P<0. 05);(3)Stepwise regression analysis showed that TXNIP level was independently correlated with ISI and BMI( P < 0. 05).Conclusion The elevation of serum TXNIP in T2 DM patients may be involved in insulin resistance and islet cell apoptosis.
引文
[1]国际糖尿病联盟.糖尿病地图[EB/OL].(2015-12-01)[2016-04-07]. https://www. useit. com. cn/forum.php? mod=viewthread&tid=11851.
[2] Chen KS,Deluca HF. Isolation and characterization of a novel c DNA from HL-60 cells treated with 1,25-dihydroxyvi-tamin D-3[J]. Biochimica Biophysica Acta,1994,1 219(1):26-32.
[3] Nishiyama A,Matsui M,Iwata S,et al.Identification of thioredoxin-binding protein-2/vitamin D 3 up-regulated protein1 as a negative regulator of thioredoxin function and expression[J].J Biol Chem,1999,274(31):21 645-21 650.
[4] Parikh H,Carlsson E,Chutkow WA,et al.TXNIP Regulates Peripheral Glucose Metabolism in Humans[J].PLOS Medicine,2007,4(5):158.
[5] William A,Chutkow,Andreas L,et al.Deletion of the-Arrestin Protein TXNIP in Mice Promotes Adiposity and Adipogenesis While Preserving Insulin Sensitivity[J]. DIABETES,2010,59(6):1 424-1 434.
[6]陈燕铭.糖尿病的最新诊断标准[J].国际医药卫生导报,1999,10(5):35.
[7] Kibbe C,Chen J,Xu G,et al.FOXO1 competes with carbohydrate response element-binding protein(ChREBP)and inhibits thioredoxin-interacting protein(TXNIP)transcription in pancreatic beta cells[J]. J Biol Chem,2013,288(32):23 194-23 202.
[8]吴平,杜冠华.治疗糖尿病及其血管并发症的潜在新靶点-TXNIP[J].药学学报,2015,50(12):1 559-1 564.
[9] Filios SR,Xu G,Chen J,et al.MicroRNA-200 is induced by thioredoxin-interacting protein and regulates Zeb1 protein signaling and Beta cell apoptosis[J]. J Biol Chem,2014,289(52):36 275-36 283.
[10]郭莹族,谭丽艳,王一舒.新诊断2型糖尿病患者血清CTRP3与Omentin-1的水平变化与肥胖相关性胰岛素抵抗的关系[J].湖北民族学院学报·医学版,2018,35(4):10-13.
[2] Chen KS,Deluca HF. Isolation and characterization of a novel c DNA from HL-60 cells treated with 1,25-dihydroxyvi-tamin D-3[J]. Biochimica Biophysica Acta,1994,1 219(1):26-32.
[3] Nishiyama A,Matsui M,Iwata S,et al.Identification of thioredoxin-binding protein-2/vitamin D 3 up-regulated protein1 as a negative regulator of thioredoxin function and expression[J].J Biol Chem,1999,274(31):21 645-21 650.
[4] Parikh H,Carlsson E,Chutkow WA,et al.TXNIP Regulates Peripheral Glucose Metabolism in Humans[J].PLOS Medicine,2007,4(5):158.
[5] William A,Chutkow,Andreas L,et al.Deletion of the-Arrestin Protein TXNIP in Mice Promotes Adiposity and Adipogenesis While Preserving Insulin Sensitivity[J]. DIABETES,2010,59(6):1 424-1 434.
[6]陈燕铭.糖尿病的最新诊断标准[J].国际医药卫生导报,1999,10(5):35.
[7] Kibbe C,Chen J,Xu G,et al.FOXO1 competes with carbohydrate response element-binding protein(ChREBP)and inhibits thioredoxin-interacting protein(TXNIP)transcription in pancreatic beta cells[J]. J Biol Chem,2013,288(32):23 194-23 202.
[8]吴平,杜冠华.治疗糖尿病及其血管并发症的潜在新靶点-TXNIP[J].药学学报,2015,50(12):1 559-1 564.
[9] Filios SR,Xu G,Chen J,et al.MicroRNA-200 is induced by thioredoxin-interacting protein and regulates Zeb1 protein signaling and Beta cell apoptosis[J]. J Biol Chem,2014,289(52):36 275-36 283.
[10]郭莹族,谭丽艳,王一舒.新诊断2型糖尿病患者血清CTRP3与Omentin-1的水平变化与肥胖相关性胰岛素抵抗的关系[J].湖北民族学院学报·医学版,2018,35(4):10-13.