血小板捐献者血型资料数据库的建设与应用展望
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摘要
供受者血小板配合输注可有效解决免疫性血小板输注无效问题。由于受HLA和HPA基因系统多态性、人群中CD36抗原缺乏个体比例及临床应用需求时限性等因素的影响,需要提前建立有一定规模的血小板捐献者血型资料库,才可能在临床需要时及时提供配合性血小板。血小板捐献者血型资料库涉及HLA-A,-B基因型资料库、HPA基因型资料库和CD36抗原阴性资料库;国内部分血站已建立200—4 000(人)份捐献者血小板血型资料库。建库中献血者选择、HLA-C位点覆盖问题、HPA系统最适检测范围和检测方法选择仍有待标准化。血小板捐献者血型资料库具有良好的应用前景,但仍需要通过扩大血小板捐献者基因型资料库规模、缩短配合血小板制备发放流程所需时间和加强临床沟通等措施,来提升基因型配合血小板的临床应用。
It was reported that the match compatible platelet products between the donor and recipient can be used for effectively solve the platelet transfusion refractoriness by immunological factors. The polymorphism of HLA and HPAs system, the proportion of CD36 antigen deficient individuals and the time limit of clinical application can be affected to obtain the match compatible platelet products in time. Therefore, to provide the match compatible platelet products in time for clinical usage, it need to establish the databases of platelet donors with a certain scale ahead of time. The database of platelet donors are included the HLA-A,-B genotype database, HPA genotype database and CD36 antigen negative database. Some databases with 200 to 4000 platelet donors have been established in some blood services in China. However, the chosen of platelet donors, HLA-C loci detection, the detection scope of HPA system and the selection of method need to be standardized. The databases of platelet donors has good application prospects. In order to enhance the clinical usage of the match compatible platelets, it is still necessary to enlarge the scale of the database of platelet donors, shorten the time for platelet preparation and release, and strengthen clinical communication.
It was reported that the match compatible platelet products between the donor and recipient can be used for effectively solve the platelet transfusion refractoriness by immunological factors. The polymorphism of HLA and HPAs system, the proportion of CD36 antigen deficient individuals and the time limit of clinical application can be affected to obtain the match compatible platelet products in time. Therefore, to provide the match compatible platelet products in time for clinical usage, it need to establish the databases of platelet donors with a certain scale ahead of time. The database of platelet donors are included the HLA-A,-B genotype database, HPA genotype database and CD36 antigen negative database. Some databases with 200 to 4000 platelet donors have been established in some blood services in China. However, the chosen of platelet donors, HLA-C loci detection, the detection scope of HPA system and the selection of method need to be standardized. The databases of platelet donors has good application prospects. In order to enhance the clinical usage of the match compatible platelets, it is still necessary to enlarge the scale of the database of platelet donors, shorten the time for platelet preparation and release, and strengthen clinical communication.
引文
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[3] Stanworth SJ,Navarrete C,Estcourt L,et al.Platelet refractoriness——practical approaches and ongoing dilemmas in patient management.Br J Haematol,2015 ,171(3):297-305.
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[5] Vassallo RR,Fung M,Rebulla P,et al.Utility of cross-matched platelet transfusions in patients with hypoproliferative thrombocytopenia:a systematic review.Transfusion,2014,54(4):1180-1191.
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[11] Xu X,Li L,Xia W,et al.Successful management of a hydropic fetus with severe anemia and thrombocytopenia caused by anti-CD36 antibody.Int J Hematol,2018,107(2):251-256.
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[14] Hauck-Dlimi B,Hammon K,Eckstein R,et al.Human platelet antigen genotypes in Turkish and Caucasian blood donors in Germany.Tissue Antigens,2012,80(3):214-218.
[15] Tan JY,Lian LH,Nadarajan VS.Genetic polymorphisms of human platelet antigens-1 to -6,and -15 in the Malaysian population.Blood Transfus,2012,10(3):368-376.
[16] Hong X,Chen S,Ying Y,et al.Simultaneous genotyping of human platelet alloantigen-1 to 28bw systems by multiplex polymerase chain reaction sequence-based typing.Vox Sang,2017,112(4):360-366.
[17] Xu X,Liu Y,Hong X,et al.Variants of CD36 gene and their association with CD36 protein expression in platelets.Blood Transfus,2014,12(4):557-564.
[18] 丁浩强,徐秀章,王嘉励,等.Ⅱ型CD36缺乏症基因遗传不对称性.基础医学与临床,2016,36(5):594-597.
[19] Saw CL,Szykoluk H,Curtis BR,et al.Two cases of platelet transfusion refractoriness associated with anti-CD36.Transfusion,2010 ,50(12):2638-2642.
[20] 张志升.基于抗原表位的血小板配型研究.中国输血杂志,2010,23(10):850-851.
[21] Davey S,Ord J,Navarrete C,et al.HLA-A,-B and -C allele and haplotype frequencies defined by next generation sequencing in a population of 519 English blood donors.Hum Immunol,2017 ,78(5-6):397-398.
[22] Chen N,Wang W,Wang F,et al.The distributions of HLA-A,HLA-B,HLA-C,HLA-DRB1 and HLA-DQB1 allele and haplotype at high-resolution level in Zhejiang Han population of China.Int J Immunogenet,2019 ,46(1):7-16.
[23] Bub CB,Torres MA,Moraes ME,et al.Determination of an unrelated donor pool size for human leukocyte antigen-matched platelets in Brazil.Rev Bras Hematol Hemoter,2016,38(1):1-6.
[24] Portela CN,Schriefer A,Albuquerque SR,et al.The human platelet alloantigen profile in blood donors from Amazonas,Brazil.Transfus Med,2016,26(6):448-456.
[25] Pai SC,Burnouf T,Chen JW,et al.Human platelet antigen alleles in 998 Taiwanese blood donors determined by sequence-specific primer polymerase chain reaction.Biomed Res Int,2013:doi:10.1155/2013/973789.
[26] Feng ML,Liu DZ,Shen W,et al.Establishment of an HPA-1- to -16-typed platelet donor registry in China.Transfus Med,2006,16(5):369-374.
[27] 马开荣,洪小珍,陈舒,等.血站系统单采血小板献血者HLA和HPA基因型资料库的调查和分析.中国输血杂志,2018,
[28] 刘静,徐秀章,丁浩强,等.广州地区无偿献血者的CD36缺失频率及其分子基础研究.中国输血杂志,2018,31(10):1132-1135.
[29] 钟周琳,申卫东,周燕,等.广西地区汉、壮和瑶族人群CD36缺失结构实验分析和特征研究.中国输血杂志,2014,27(01):14-17.
[30] 陈青,段志敏,蔡莉,等.江苏地区汉族人群血小板CD36抗原缺失型表型的分析.中国医药生物技术,2016,11(01):38-41.
[31] Apps R,Qi Y,Carlson JM,et al.Influence of HLA-C expression level on HIV control.Science,2013,340(6128):87-91.
[32]韩瑜,杨帆,于晓丽,等.血小板输注效果和HLA-C基因的关联性研究.中国输血杂志,2018,
[33] Kengkate M,Butthep P,Kupatawintu P,et al.Comparison of a simple-probe real-time PCR and multiplex PCR techniques for HPA-1 to HPA-6 and HPA-15 genotyping.J Clin Lab Anal,2015,29(2):94-99.
[34] Merzoni J,Fagundes IS,Lunardi LW,et al.Human platelet antigen genotyping of platelet donors in southern Brazil.Int J Immunogenet,2015 ,42(5):329-335.
[35] Zhou H,Ding H,Chen Y,et al.Simultaneous genotyping of HPA-17w to -21w by PCR-SSP in Chinese Cantonese.Platelets,2015,26(2):18 618-18 619.
[36] 李茵,苏蔓,何路军,等.石家庄汉族血小板捐献者HPA 1-17基因多态性分析.中国输血杂志,2018.
[2] Wu G,Zhou Y,Li L,et al.Platelet immunology in China:research and clinical applications.Transfus Med Rev,2017,31(2):118-125.
[3] Stanworth SJ,Navarrete C,Estcourt L,et al.Platelet refractoriness——practical approaches and ongoing dilemmas in patient management.Br J Haematol,2015 ,171(3):297-305.
[4] Juskewitch JE,Norgan AP,de Goey SR,et al.How do I … manage the platelet transfusion-refractory patient?Transfusion,2017,57(12):2828-2835.
[5] Vassallo RR,Fung M,Rebulla P,et al.Utility of cross-matched platelet transfusions in patients with hypoproliferative thrombocytopenia:a systematic review.Transfusion,2014,54(4):1180-1191.
[6] Wang J,Xia W,Deng J,et al.Analysis of platelet-reactive alloantibodies and evaluation of cross-match-compatible platelets for the management of patients with transfusion refractoriness.Transfus Med,2018,28(1):40-46.
[7] Valsami S,Dimitroulis D,Gialeraki A,et al.Current trends in platelet transfusions practice:The role of ABO-RhD and human leukocyte antigen incompatibility.Asian J Transfus Sci,2015,9(2):117-123.
[8] Karlstr?m C,Linjama T,Edgren G,et al.HLA-selected platelets for platelet refractory patients with HLA antibodies:a single-center experience.Transfusion,2019,59(3):945-952.
[9] Sullivan MJ,Peterson J,McFarland JG,et al.A new low-frequency alloantigen (Kha(b) ) located on platelet glycoprotein IIIa as acause of maternal sensitization leading to neonatal alloimmune thrombocytopenia.Transfusion,2015,55(6Pt 2):1584-1585.
[10] Xu X,Ye X,Xia W,et al.Studies on CD36 deficiency in South China:Two cases demonstrating the clinical impact of anti-CD36 antibodies.Thromb Haemost,2013,110(6):1199-1206.
[11] Xu X,Li L,Xia W,et al.Successful management of a hydropic fetus with severe anemia and thrombocytopenia caused by anti-CD36 antibody.Int J Hematol,2018,107(2):251-256.
[12] He Y,Li J,Mao W,et al.HLA common and well-documented alleles in China.HLA,2018,92(4):199-205.
[13] Silvestre APA,Zacarias JMV,Guelsin GAS,et al.Genetic polymorphisms of human platelet antigens in Euro-African and Japanese descendants from Parana,Southern Brazil.Platelets,2017,28(6):607-610.
[14] Hauck-Dlimi B,Hammon K,Eckstein R,et al.Human platelet antigen genotypes in Turkish and Caucasian blood donors in Germany.Tissue Antigens,2012,80(3):214-218.
[15] Tan JY,Lian LH,Nadarajan VS.Genetic polymorphisms of human platelet antigens-1 to -6,and -15 in the Malaysian population.Blood Transfus,2012,10(3):368-376.
[16] Hong X,Chen S,Ying Y,et al.Simultaneous genotyping of human platelet alloantigen-1 to 28bw systems by multiplex polymerase chain reaction sequence-based typing.Vox Sang,2017,112(4):360-366.
[17] Xu X,Liu Y,Hong X,et al.Variants of CD36 gene and their association with CD36 protein expression in platelets.Blood Transfus,2014,12(4):557-564.
[18] 丁浩强,徐秀章,王嘉励,等.Ⅱ型CD36缺乏症基因遗传不对称性.基础医学与临床,2016,36(5):594-597.
[19] Saw CL,Szykoluk H,Curtis BR,et al.Two cases of platelet transfusion refractoriness associated with anti-CD36.Transfusion,2010 ,50(12):2638-2642.
[20] 张志升.基于抗原表位的血小板配型研究.中国输血杂志,2010,23(10):850-851.
[21] Davey S,Ord J,Navarrete C,et al.HLA-A,-B and -C allele and haplotype frequencies defined by next generation sequencing in a population of 519 English blood donors.Hum Immunol,2017 ,78(5-6):397-398.
[22] Chen N,Wang W,Wang F,et al.The distributions of HLA-A,HLA-B,HLA-C,HLA-DRB1 and HLA-DQB1 allele and haplotype at high-resolution level in Zhejiang Han population of China.Int J Immunogenet,2019 ,46(1):7-16.
[23] Bub CB,Torres MA,Moraes ME,et al.Determination of an unrelated donor pool size for human leukocyte antigen-matched platelets in Brazil.Rev Bras Hematol Hemoter,2016,38(1):1-6.
[24] Portela CN,Schriefer A,Albuquerque SR,et al.The human platelet alloantigen profile in blood donors from Amazonas,Brazil.Transfus Med,2016,26(6):448-456.
[25] Pai SC,Burnouf T,Chen JW,et al.Human platelet antigen alleles in 998 Taiwanese blood donors determined by sequence-specific primer polymerase chain reaction.Biomed Res Int,2013:doi:10.1155/2013/973789.
[26] Feng ML,Liu DZ,Shen W,et al.Establishment of an HPA-1- to -16-typed platelet donor registry in China.Transfus Med,2006,16(5):369-374.
[27] 马开荣,洪小珍,陈舒,等.血站系统单采血小板献血者HLA和HPA基因型资料库的调查和分析.中国输血杂志,2018,
[28] 刘静,徐秀章,丁浩强,等.广州地区无偿献血者的CD36缺失频率及其分子基础研究.中国输血杂志,2018,31(10):1132-1135.
[29] 钟周琳,申卫东,周燕,等.广西地区汉、壮和瑶族人群CD36缺失结构实验分析和特征研究.中国输血杂志,2014,27(01):14-17.
[30] 陈青,段志敏,蔡莉,等.江苏地区汉族人群血小板CD36抗原缺失型表型的分析.中国医药生物技术,2016,11(01):38-41.
[31] Apps R,Qi Y,Carlson JM,et al.Influence of HLA-C expression level on HIV control.Science,2013,340(6128):87-91.
[32]韩瑜,杨帆,于晓丽,等.血小板输注效果和HLA-C基因的关联性研究.中国输血杂志,2018,
[33] Kengkate M,Butthep P,Kupatawintu P,et al.Comparison of a simple-probe real-time PCR and multiplex PCR techniques for HPA-1 to HPA-6 and HPA-15 genotyping.J Clin Lab Anal,2015,29(2):94-99.
[34] Merzoni J,Fagundes IS,Lunardi LW,et al.Human platelet antigen genotyping of platelet donors in southern Brazil.Int J Immunogenet,2015 ,42(5):329-335.
[35] Zhou H,Ding H,Chen Y,et al.Simultaneous genotyping of HPA-17w to -21w by PCR-SSP in Chinese Cantonese.Platelets,2015,26(2):18 618-18 619.
[36] 李茵,苏蔓,何路军,等.石家庄汉族血小板捐献者HPA 1-17基因多态性分析.中国输血杂志,2018.