水杨酸对草莓SnRK1活性及植株生长的影响
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摘要
本文以‘妙香7号’草莓(Fragaria×ananassa)为试验材料,分析其根系和叶中FaSnRK1s对水杨酸(SA)处理的响应特性,研究了SA对草莓SnRK1活性及植株生长的影响。结果表明,外源SA处理后根和叶中FaSnRK1s分别在0.5~1 h和1~2 h内表达量达到最高水平,随后均趋于下降至初始水平;较高浓度(80和100μmol·L~(-1))的SA处理后草莓功能叶及根系中SnRK1活性显著提高,且叶片净光合速率显著提高,可溶性糖和淀粉含量显著增加,盆栽草莓地上及地下部生长势明显增强。上述结果表明,SA处理短时间内提高了SnRK1各亚基编码基因的表达量,同时提高SnRK1活性,可能因此影响了植株的碳代谢,从而促进草莓植株的生长。
Strawberry(Fragaria× ananassa) ‘Miaoxiang 7' was used to investigate the expression characteristics of Fa SnRK1 s in response to salicylic acid(SA) in roots and leaves, the effect of SA on SnRK1 activity and growth of strawberry plant. Quantitative real-time PCR analysis showed that the expression of Fa SnRK1 s within 0.5–1 h reached the highest level in roots, and within 1–2 h reached the highest level in leaves, then dropped to the initial level. The treatments of 80 and 100 μmol·L~(-1) SA significantly increased SnRK1 activities, net photosynthetic rates, as well as the soluble sugar and starch contents compared to the control conditions. All these results indicated that SA enhanced the expression of Fa SnRK1 s and the activity of SnRK1 as a kinase, which might promote plant growth through carbon metabolism.
Strawberry(Fragaria× ananassa) ‘Miaoxiang 7' was used to investigate the expression characteristics of Fa SnRK1 s in response to salicylic acid(SA) in roots and leaves, the effect of SA on SnRK1 activity and growth of strawberry plant. Quantitative real-time PCR analysis showed that the expression of Fa SnRK1 s within 0.5–1 h reached the highest level in roots, and within 1–2 h reached the highest level in leaves, then dropped to the initial level. The treatments of 80 and 100 μmol·L~(-1) SA significantly increased SnRK1 activities, net photosynthetic rates, as well as the soluble sugar and starch contents compared to the control conditions. All these results indicated that SA enhanced the expression of Fa SnRK1 s and the activity of SnRK1 as a kinase, which might promote plant growth through carbon metabolism.
引文
Baena-Gonzalez E,Rolland F,Thevelein JM,et al(2007).Acentral integrator of transcription networks in plant stress and energy signalling.Nature,448(7156):938-942
Baena-Gonzalez E,Sheen J(2008).Convergent energy and stress signaling.Trends Plant Sci,13:474-482
Debast S,Nunes-Nesi A,Hajirezaei MR,et al(2011).Altering trehalose-6-phosphate content in transgenic potato tubers affects tuber growth and alters responsiveness to hormones during sprouting.Plant Physiol,156:1754-1771
Douglas P,Pigaglio E,Ferrer A,et al(1997).Three spinach leaf nitrate reductase/3-hydroxy-3-methylglutary-Co Areductase kinases that are regulated by reversible phosphorylation and/or Ca2+ions.Biochem J,325:101-109
Geigenberger P(2003).Regulation of sucrose to starch conversion in growing potato tubers.J Exp Bot,54(382):457-465
Geigenberger P,Stitt M,Fernie AR(2004).Metabolic control analysis and regulation of the conversion of sucrose to starch in growing potato tubers.Plant Cell Environ,27(6):655-673
Halford NG,Hardie DG(1998).SNF1-related protein kinases:global regulators of carbon metabolism in plants?Plant Mol Biol,37(5):735-748
Harthill JE,Meek SE,Morrice N,et al(2006).Phosphorylation and 14-3-3 binding of Arabidopsis trehalose-phosphate synthase 5 in response to 2-deoxyglucose.Plant J,47:211-223
Hawley SA,Fullerton MD,Ross FA,et al(2012).The ancient drug salicylate directly activates AMP-activated protein kinase.Science,336(6083):918-922
Huang QQ,Sun X,Zhang NH,et al(2004).Effects of salicylic acid on leaves of cucumber seedlings under water stress.Acta Bot Boreal-Occident Sin,4(12):2202-2207(in Chinese with English abstract)[黄清泉,孙歆,张年辉等(2004).水杨酸对水分胁迫黄瓜幼苗叶片生理过程的影响.西北植物学报,4(12):2202-2207]
Janda T,Gondor OK,Yordanova R,et al(2014).Salicylic acid and photosynthesis:signalling and effects.Acta Physiol Plant,36:2537-2546
Khan W,Prithiviraj B,Smith DL(2003).Photosynthetic responses of corn and soybean to foliar application of salicylates.J Plant Physiol,160(5):485-492
Laurie S,Mc Kibbin RS,Halford NG(2003).Antisense SNF1-related(Sn RK1)protein kinase gene represses transient activity of anα-amylase(α-Amy2)gene promoter in cultured wheat embryos.J Exp Bot,54(383):739-747
Li L,Dong CJ,Shang QM(2013).Role of endogenous salicylic acid in responding of cucumber leaf photosynthetic systems to low temperature stress.Acta Hortic Sin,40(3):487-497(in Chinese with English abstract)[李亮,董春娟,尚庆茂(2013).内源水杨酸参与黄瓜叶片光合系统对低温胁迫的响应.园艺学报,40(3):487-497]
Li XF,Li YJ,An YH,et al(2009).AKINβ1 is involved in the regulation of nitrogen metabolism and sugar signaling in Arabidopsis.J Integar Plant Biol,51(5):513-520
Luo MH,Yuan S,Chen YE,et al(2009).Effects of salicylic acid on the photosystem 2 of barley seedlings under osmotic stress.Biol Plant,53(4):663-669
Nunes C,O’Hara LE,Primavesi LF,et al(2013).The trehalose 6-phosphate/Sn RK1 signaling pathway primes growth recovery following relief of sink limitation.Plant Physiol,162:1720-1732
Purcell PC,Smith AM,Halford NG(1998).Antisense expression of a sucrose non-fermenting-1-related protein kinase sequence in potato results in decreased expression of sucrose synthase in tubers and loss of sucrose-inducibility of sucrose synthase transcripts in leaves.Plant J,14(2):195-202
Slaymaker DH,Navarre DA,Clark D,et al(2002).The tobacco salicylic acid-binding protein 3(SABP3)is the chloroplast carbonic anhydrase,which exhibits antioxidant activity and plays a role in the hypersensitive defense response.Proc Natl Acad Sci USA,99(18):11640-11645
Sugden C,Crawford RM,Halford NG,et al(1999a).Regulation of spinach SNF1-related(Sn RK1)kinases by protein kinases and phosphatases is associated with phosphorylation of the T loop and is regulated by 5′-AMP.Plant J,19(4):433-439
Sugden C,Donaghy PG,Halford NG,et al(1999b).Two SNF1-related protein kinases from spinach leaf phosphorylate and inactivate 3-hydroxy-3-methylglutaryl-coenzyme A reductase,nitrate reductase,and sucrose phosphate synthase in vitro.Plant Physiol,120(1):257-274
Wang GF(2014).Clonging and functional analysis of Prunus Sn RK1 alpha and beta subunits genes(dissertation).Taian:Shandong Agricultural University(in Chinese with English abstract)[王贵芳(2014).桃Sn RK1蛋白激酶α和β亚基编码基因的克隆及功能分析(学位论文).泰安:山东农业大学]
Wang GF,Peng FT,Zhang YF,et al(2014).Effects of overexpressing Pingyi Tiancha Mh Sn RK1 on carbohydrate metabolism in tomato.Acta Hortic Sin,41(11):2188-2195(in Chinese with English abstract)[王贵芳,彭福田,张亚飞等(2014).平邑甜茶Mh Sn RK1在番茄中超表达对植株碳代谢的影响.园艺学报,41(11):2188-2195]
Wang LJ,Fan L,Loescher W,et al(2010).Salicylic acid alleviates decreases in photosynthesis under heat stress and accelerates recovery in grapevine leaves.Plant Biol,10:34
Wang XL(2012).Clonging and functional characterization of key genes response to nitrate in fruit tree roots(dissertation).Taian:Shandong Agricultural University(in Chinese with English abstract)[王新亮(2012).果树根系硝态氮信号响应关键基因的克隆与功能分析(学位论文).泰安:山东农业大学]
Wang XL,Peng FT,Li MJ,et al(2012).Expression of a heterologous Sn RK1 in tomato increases carbon assimilation,nitrogen uptake and modifies fruit development.J Plant Physiol,169(12):1173-1182
Wildermuth MC,Derdney J,Wu G,et al(2001).Isochorismate synthase is required to synthesize salicylic acid for plant defense.Nature,414(6863):562-565
Wingler A,Delatte TL,O’Hara LE,et al(2012).Trehalose6-phosphate is required for the onset of leaf senescence associated with high carbon availability.Plant Physiol,158(3):1241-1251
Wingler A,Fritzius T,Wiemken A,et al(2000).Trehalose induces the ADP-glucose pyrophosphorylase gene,Ap L3,and starch synthesis in Arabidopsis.Plant Physiol,124:105-114
Yi XL,Yang BX,Zong XF,et al(2011).Signal chemical salicylic acid mitigates the negative effects of drought onphotosynthesis and membrance lipid peroxidation of purple majesty.Acta Ecol Sin,31(1):67-74(in Chinese with English abstract)[易小林,杨丙贤,宗学凤等(2011).信号分子水杨酸减缓干旱胁迫对紫御谷光合和膜脂过氧化的副效应.生态学报,31(1):67-74]
Zhang W,Wang YF,Guo YP(2016).Review on crosstalk regulation involving in trehalose-6-phosphate signal in plant.Plant Physiol J,52(4):394-400(in Chinese with English abstract)[张雯,王宇斐,郭延平(2016).高等植物6-磷酸海藻糖信号调控研究进展.植物生理学报,52(4):394-400]
Zhang YH,Primavesi LF,Jhurreea D,et al(2009).Inhibition of SNF1-related protein kinase1 activity and regulation of metabolic pathways by trehalose-6-phosphate.Plant Physiol,149:1860-1871
Zhao SJ,Shi GA,Dong XC(2002).Techniques of Plant Physiological Experiment.Beijing:Chinese Agricultural Science and Technology Press(in Chinese)[赵世杰,史国安,董新纯(2002).植物生理学实验指导.北京:中国农业科学技术出版社]
Zhao YF,Chen XL,Peng FT,et al(2017).Ovesr expression of a peach Pp Sn RK1βγ1 gene improving oxidative stress tolerance in Arabidopsis thaliana.Plant Physiol J,53(7):1251-1258(in Chinese with English abstract)[赵永飞,陈晓璐,彭福田等(2017).桃Pp Sn RK1βγ1在拟南芥中超表达提高植株氧化胁迫耐性.植物生理学报,53(7):1251-1258]
Baena-Gonzalez E,Sheen J(2008).Convergent energy and stress signaling.Trends Plant Sci,13:474-482
Debast S,Nunes-Nesi A,Hajirezaei MR,et al(2011).Altering trehalose-6-phosphate content in transgenic potato tubers affects tuber growth and alters responsiveness to hormones during sprouting.Plant Physiol,156:1754-1771
Douglas P,Pigaglio E,Ferrer A,et al(1997).Three spinach leaf nitrate reductase/3-hydroxy-3-methylglutary-Co Areductase kinases that are regulated by reversible phosphorylation and/or Ca2+ions.Biochem J,325:101-109
Geigenberger P(2003).Regulation of sucrose to starch conversion in growing potato tubers.J Exp Bot,54(382):457-465
Geigenberger P,Stitt M,Fernie AR(2004).Metabolic control analysis and regulation of the conversion of sucrose to starch in growing potato tubers.Plant Cell Environ,27(6):655-673
Halford NG,Hardie DG(1998).SNF1-related protein kinases:global regulators of carbon metabolism in plants?Plant Mol Biol,37(5):735-748
Harthill JE,Meek SE,Morrice N,et al(2006).Phosphorylation and 14-3-3 binding of Arabidopsis trehalose-phosphate synthase 5 in response to 2-deoxyglucose.Plant J,47:211-223
Hawley SA,Fullerton MD,Ross FA,et al(2012).The ancient drug salicylate directly activates AMP-activated protein kinase.Science,336(6083):918-922
Huang QQ,Sun X,Zhang NH,et al(2004).Effects of salicylic acid on leaves of cucumber seedlings under water stress.Acta Bot Boreal-Occident Sin,4(12):2202-2207(in Chinese with English abstract)[黄清泉,孙歆,张年辉等(2004).水杨酸对水分胁迫黄瓜幼苗叶片生理过程的影响.西北植物学报,4(12):2202-2207]
Janda T,Gondor OK,Yordanova R,et al(2014).Salicylic acid and photosynthesis:signalling and effects.Acta Physiol Plant,36:2537-2546
Khan W,Prithiviraj B,Smith DL(2003).Photosynthetic responses of corn and soybean to foliar application of salicylates.J Plant Physiol,160(5):485-492
Laurie S,Mc Kibbin RS,Halford NG(2003).Antisense SNF1-related(Sn RK1)protein kinase gene represses transient activity of anα-amylase(α-Amy2)gene promoter in cultured wheat embryos.J Exp Bot,54(383):739-747
Li L,Dong CJ,Shang QM(2013).Role of endogenous salicylic acid in responding of cucumber leaf photosynthetic systems to low temperature stress.Acta Hortic Sin,40(3):487-497(in Chinese with English abstract)[李亮,董春娟,尚庆茂(2013).内源水杨酸参与黄瓜叶片光合系统对低温胁迫的响应.园艺学报,40(3):487-497]
Li XF,Li YJ,An YH,et al(2009).AKINβ1 is involved in the regulation of nitrogen metabolism and sugar signaling in Arabidopsis.J Integar Plant Biol,51(5):513-520
Luo MH,Yuan S,Chen YE,et al(2009).Effects of salicylic acid on the photosystem 2 of barley seedlings under osmotic stress.Biol Plant,53(4):663-669
Nunes C,O’Hara LE,Primavesi LF,et al(2013).The trehalose 6-phosphate/Sn RK1 signaling pathway primes growth recovery following relief of sink limitation.Plant Physiol,162:1720-1732
Purcell PC,Smith AM,Halford NG(1998).Antisense expression of a sucrose non-fermenting-1-related protein kinase sequence in potato results in decreased expression of sucrose synthase in tubers and loss of sucrose-inducibility of sucrose synthase transcripts in leaves.Plant J,14(2):195-202
Slaymaker DH,Navarre DA,Clark D,et al(2002).The tobacco salicylic acid-binding protein 3(SABP3)is the chloroplast carbonic anhydrase,which exhibits antioxidant activity and plays a role in the hypersensitive defense response.Proc Natl Acad Sci USA,99(18):11640-11645
Sugden C,Crawford RM,Halford NG,et al(1999a).Regulation of spinach SNF1-related(Sn RK1)kinases by protein kinases and phosphatases is associated with phosphorylation of the T loop and is regulated by 5′-AMP.Plant J,19(4):433-439
Sugden C,Donaghy PG,Halford NG,et al(1999b).Two SNF1-related protein kinases from spinach leaf phosphorylate and inactivate 3-hydroxy-3-methylglutaryl-coenzyme A reductase,nitrate reductase,and sucrose phosphate synthase in vitro.Plant Physiol,120(1):257-274
Wang GF(2014).Clonging and functional analysis of Prunus Sn RK1 alpha and beta subunits genes(dissertation).Taian:Shandong Agricultural University(in Chinese with English abstract)[王贵芳(2014).桃Sn RK1蛋白激酶α和β亚基编码基因的克隆及功能分析(学位论文).泰安:山东农业大学]
Wang GF,Peng FT,Zhang YF,et al(2014).Effects of overexpressing Pingyi Tiancha Mh Sn RK1 on carbohydrate metabolism in tomato.Acta Hortic Sin,41(11):2188-2195(in Chinese with English abstract)[王贵芳,彭福田,张亚飞等(2014).平邑甜茶Mh Sn RK1在番茄中超表达对植株碳代谢的影响.园艺学报,41(11):2188-2195]
Wang LJ,Fan L,Loescher W,et al(2010).Salicylic acid alleviates decreases in photosynthesis under heat stress and accelerates recovery in grapevine leaves.Plant Biol,10:34
Wang XL(2012).Clonging and functional characterization of key genes response to nitrate in fruit tree roots(dissertation).Taian:Shandong Agricultural University(in Chinese with English abstract)[王新亮(2012).果树根系硝态氮信号响应关键基因的克隆与功能分析(学位论文).泰安:山东农业大学]
Wang XL,Peng FT,Li MJ,et al(2012).Expression of a heterologous Sn RK1 in tomato increases carbon assimilation,nitrogen uptake and modifies fruit development.J Plant Physiol,169(12):1173-1182
Wildermuth MC,Derdney J,Wu G,et al(2001).Isochorismate synthase is required to synthesize salicylic acid for plant defense.Nature,414(6863):562-565
Wingler A,Delatte TL,O’Hara LE,et al(2012).Trehalose6-phosphate is required for the onset of leaf senescence associated with high carbon availability.Plant Physiol,158(3):1241-1251
Wingler A,Fritzius T,Wiemken A,et al(2000).Trehalose induces the ADP-glucose pyrophosphorylase gene,Ap L3,and starch synthesis in Arabidopsis.Plant Physiol,124:105-114
Yi XL,Yang BX,Zong XF,et al(2011).Signal chemical salicylic acid mitigates the negative effects of drought onphotosynthesis and membrance lipid peroxidation of purple majesty.Acta Ecol Sin,31(1):67-74(in Chinese with English abstract)[易小林,杨丙贤,宗学凤等(2011).信号分子水杨酸减缓干旱胁迫对紫御谷光合和膜脂过氧化的副效应.生态学报,31(1):67-74]
Zhang W,Wang YF,Guo YP(2016).Review on crosstalk regulation involving in trehalose-6-phosphate signal in plant.Plant Physiol J,52(4):394-400(in Chinese with English abstract)[张雯,王宇斐,郭延平(2016).高等植物6-磷酸海藻糖信号调控研究进展.植物生理学报,52(4):394-400]
Zhang YH,Primavesi LF,Jhurreea D,et al(2009).Inhibition of SNF1-related protein kinase1 activity and regulation of metabolic pathways by trehalose-6-phosphate.Plant Physiol,149:1860-1871
Zhao SJ,Shi GA,Dong XC(2002).Techniques of Plant Physiological Experiment.Beijing:Chinese Agricultural Science and Technology Press(in Chinese)[赵世杰,史国安,董新纯(2002).植物生理学实验指导.北京:中国农业科学技术出版社]
Zhao YF,Chen XL,Peng FT,et al(2017).Ovesr expression of a peach Pp Sn RK1βγ1 gene improving oxidative stress tolerance in Arabidopsis thaliana.Plant Physiol J,53(7):1251-1258(in Chinese with English abstract)[赵永飞,陈晓璐,彭福田等(2017).桃Pp Sn RK1βγ1在拟南芥中超表达提高植株氧化胁迫耐性.植物生理学报,53(7):1251-1258]