钼在桃树干旱胁迫响应中的作用解析
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摘要
以毛桃(Amygdalus persica)实生苗为试材,研究干旱胁迫下,钼酸铵处理对钼辅因子硫化酶编码基因(LOS5/ABA3)表达量、脱落酸(ABA)含量及抗旱相关生理指标的影响。结果表明,干旱胁迫下,喷施不同浓度钼酸铵处理毛桃实生苗叶片,其含水量及叶绿素和脯氨酸含量显著高于对照,且以0.04%钼酸铵处理效果最好;电解质渗漏率显著低于对照。干旱胁迫下,与对照相比,喷施0.04%钼酸铵的毛桃实生苗叶片中LOS5/ABA3表达量显著提高; ABA含量、水分利用效率和净光合速率均高于对照,蒸腾速率低于对照,且差异显著;叶片抗氧化酶活性显著升高, MDA含量显著降低;离体处理的叶片质量损失减缓,且差异显著。研究表明毛桃实生苗在干旱胁迫下喷施钼酸铵可通过上调钼辅因子硫化酶编码基因的表达水平,提高叶片中ABA和脯氨酸含量及抗氧化酶活性,从而缓解干旱胁迫下的细胞膜氧化伤害,降低叶片失水速率,减轻干旱胁迫对毛桃实生苗的伤害。
Peach(Amygdalus persica) seedlings were used to analyze the effect of ammonium molybdate on the expression of molybdenum cofactor sulfurase gene(LOS5/ABA3), ABA content and physiological indexes related to drought resistance under drought stress. Water, chlorophyll and proline contents of peach seedling leaves were significantly higher with ammonium molybdate, preferably 0.04%, than control treatment, and electrolyte leakage was lower. Under drought stress, the expression of LOS5/ABA3 in leaves treated with 0.04% ammonium molybdate was significantly increased and ABA content, water use efficiency, and net photosynthetic rate was higher than with control treatment. Transpiration rate was lower, antioxidant enzyme activity was higher, and malondialdehyde content was lower in leaves; the quality loss of leaves in vitro was reduced. Thus, ammonium molybdate treatment of peach seedlings can increase the content of ABA and proline, increase the activity of antioxidant enzymes, relieve cell membrane oxidation damage, decrease the rate of water loss of leaves, and alleviate drought stress damage by regulating the expression of droughtresistant genes, then increase the drought resistance of plants.
Peach(Amygdalus persica) seedlings were used to analyze the effect of ammonium molybdate on the expression of molybdenum cofactor sulfurase gene(LOS5/ABA3), ABA content and physiological indexes related to drought resistance under drought stress. Water, chlorophyll and proline contents of peach seedling leaves were significantly higher with ammonium molybdate, preferably 0.04%, than control treatment, and electrolyte leakage was lower. Under drought stress, the expression of LOS5/ABA3 in leaves treated with 0.04% ammonium molybdate was significantly increased and ABA content, water use efficiency, and net photosynthetic rate was higher than with control treatment. Transpiration rate was lower, antioxidant enzyme activity was higher, and malondialdehyde content was lower in leaves; the quality loss of leaves in vitro was reduced. Thus, ammonium molybdate treatment of peach seedlings can increase the content of ABA and proline, increase the activity of antioxidant enzymes, relieve cell membrane oxidation damage, decrease the rate of water loss of leaves, and alleviate drought stress damage by regulating the expression of droughtresistant genes, then increase the drought resistance of plants.
引文
李洁,列志旸,薛立,黄威龙,许建新(2016).干旱胁迫对3种园林绿化树种幼苗生理指标的影响.西南林业大学学报36(2),56-61.
邵惠芳,陈征,许嘉阳,范艺宽,黄五星,张海枞,许自成(2016).两种烟草幼苗叶片对不同强度干旱胁迫的生理响应比较.植物生理学报52,1861-1871.
宋哲,章家长,楮庆全,李召虎(2008).转LOS5/ABA3基因早熟禾(P.pratensis L.)的获得及抗旱和抗寒性分析.中国科技论文在线.http://www.paper.edu.cn/releasepaper/content/200805-82.
苏金,朱汝财(2001).渗透胁迫调节的转基因表达对植物抗旱耐盐性的影响.植物学通报18,129-136.
孙学成(2006).钼提高冬小麦抗寒力的生理基础及分子机制.博士论文.武汉:华中农业大学.
王华,王飞,陈登文,丁勤(2000).低温胁迫对杏花SOD活性和膜脂过氧化的影响.果树科学17,197-201.
王尉,乐胜峰,贺天雨,杜宁,林楠(2018).高效液相色谱法测定苜蓿中脱落酸的含量.分析仪器53(2),53-57.
吴明才,肖昌珍(1994).大豆钼素研究.大豆科学13,245-251.
武松伟,胡承孝,谭启玲,孙学成(2016).钼与植物抗寒性研究进展.湖北农业科学55,13-16,42.
张士功,刘国栋,刘更另(2001).植物营养与作物抗旱性.植物学通报18,64-69,63.
张志良,瞿伟菁,李小方(2009).植物生理学实验指导(第4版).北京:高等教育出版社.pp.4-5.
赵世杰,史国安,董新纯(2002).植物生理学实验指导.北京:中国农业科学技术出版社.pp.164-165.
Atwal PS,Scaglia F(2016).Molybdenum cofactor deficiency.Mol Genet Metab 117,1-4.
Barker R,Dawe D,Tuong TP,Bhuiyan SI,Guerra LC(1998).The outlook for water resources in the Year 2020:challenges for research on water management in rice production.International Rice Commission Newsletter 49,7-21.
Garbero M,Pedranzani H,Zirulnik F,Molina A,PérezChaca MV,Vigliocco A,Abdala G(2011).Short-term cold stress in two cultivars of Digitaria eriantha:effects on stress-related hormones and antioxidant defense system.Acta Physiol Plant 33,497-507.
Hanks RJ(1983).Yield and water-use relationships:an overview.In:Taylor HM,Jordan WR,Sinclair TR,eds.Limitations to Efficient Water Use in Crop Production.Madison:American Society of Agronomy.pp.393-411.
Hille R,Hall J,Basu P(2014).The mononuclear molybdenum enzymes.Chem Rev 114,3963-4038.
Li YJ,Zhang JC,Zhang J,Hao L,Hua JP,Duan LS,Zhang MC,Li ZH(2013).Expression of an Arabidopsis molybdenum cofactor sulphurase gene in soybean enhances drought tolerance and increases yield under field conditions.Plant Biotechnol J 11,747-758.
Lu Y,Li YJ,Zhang JC,Xiao YT,Yue YS,Duan LS,Zhang MC,Li ZH(2013).Overexpression of Arabidopsis molybdenum cofactor sulfurase gene confers drought tolerance in maize(Zea mays L.).PLo S One 8,e52126.
Ma FF,Lu R,Liu HY,Shi B,Zhang JH,Tan MP,Zhang AY,Jiang MY(2012).Nitric oxide-activated calcium/calmodulin-dependent protein kinase regulates the abscisic acid-induced antioxidant defence in maize.J Exp Bot 63,4835-4847.
Mahajan S,Tuteja N(2005).Cold,salinity and drought stresses:an overview.Arch Biochem Biophys 444,139-158.
Patnaitk D,Khurana P(2002).Wheat biotechnology:a minireview.Electron J Biotechnol 4,74-102.
Sun XC,Hu CX,Tan QL,Liu JS,Liu HE(2009).Effects of molybdenum on expression of cold-responsive genes in abscisic acid(ABA)-dependent and ABA-independent pathways in winter wheat under low-temperature stress.Ann Bot 104,345-356.
Wilkinson S,Davies WJ(2002).ABA-based chemical signaling:the co-ordination of responses to stress in plants.Plant Cell Environ 25,195-210.
Wu SW,Hu CX,Tan QL,Nie ZJ,Sun XC(2014).Effects of molybdenum on water utilization,antioxidative defense system and osmotic-adjustment ability in winter wheat(Triticum aestivum)under drought stress.Plant Physiol Biochem 83,365-374.
Xiong LM,Ishitani M,Lee H,Zhu JK(2001).The Arabidopsis LOS5/ABA3 locus encodes a molybdenum cofactor sulfurase and modulates cold stress-and osmotic stress-responsive gene expression.Plant Cell 13,2063-2084.
Xiong LM,Zhu JK(2001).Abiotic stress signal transduction in plants:molecular and genetic perspectives.Physiol Plant 112,152-166.
Yue SY,Ming CZ,Zhang JC,Tian XL,Duan LS,Li ZH(2012).Overexpression of the AtLOS5 gene increased abscisic acid level and drought tolerance in transgenic cotton.J Exp Bot 63,3741-3748.
Yue YS,Zhang MC,Zhang JC,Duan LS,Li ZH(2011).Arabidopsis LOS5/ABA3 overexpression in transgenic tobacco(Nicotiana tabacum cv.‘Xanthi-nc’)results in enhanced drought tolerance.Plant Sci 181,405-411.
Zhang M,Hu CX,Zhao XH,Tan QL,Sun XC,Cao AY,Cui M,Zhang Y(2012).Molybdenum improves antioxidant and osmotic-adjustment ability against salt stress in Chinese cabbage(Brassica campestris L.ssp.pekinensis).Plant Soil 355,375-383.
Zhu JK(2002).Salt and drought stress signal transduction in plants.Annu Rev Plant Biol 53,247-273.
邵惠芳,陈征,许嘉阳,范艺宽,黄五星,张海枞,许自成(2016).两种烟草幼苗叶片对不同强度干旱胁迫的生理响应比较.植物生理学报52,1861-1871.
宋哲,章家长,楮庆全,李召虎(2008).转LOS5/ABA3基因早熟禾(P.pratensis L.)的获得及抗旱和抗寒性分析.中国科技论文在线.http://www.paper.edu.cn/releasepaper/content/200805-82.
苏金,朱汝财(2001).渗透胁迫调节的转基因表达对植物抗旱耐盐性的影响.植物学通报18,129-136.
孙学成(2006).钼提高冬小麦抗寒力的生理基础及分子机制.博士论文.武汉:华中农业大学.
王华,王飞,陈登文,丁勤(2000).低温胁迫对杏花SOD活性和膜脂过氧化的影响.果树科学17,197-201.
王尉,乐胜峰,贺天雨,杜宁,林楠(2018).高效液相色谱法测定苜蓿中脱落酸的含量.分析仪器53(2),53-57.
吴明才,肖昌珍(1994).大豆钼素研究.大豆科学13,245-251.
武松伟,胡承孝,谭启玲,孙学成(2016).钼与植物抗寒性研究进展.湖北农业科学55,13-16,42.
张士功,刘国栋,刘更另(2001).植物营养与作物抗旱性.植物学通报18,64-69,63.
张志良,瞿伟菁,李小方(2009).植物生理学实验指导(第4版).北京:高等教育出版社.pp.4-5.
赵世杰,史国安,董新纯(2002).植物生理学实验指导.北京:中国农业科学技术出版社.pp.164-165.
Atwal PS,Scaglia F(2016).Molybdenum cofactor deficiency.Mol Genet Metab 117,1-4.
Barker R,Dawe D,Tuong TP,Bhuiyan SI,Guerra LC(1998).The outlook for water resources in the Year 2020:challenges for research on water management in rice production.International Rice Commission Newsletter 49,7-21.
Garbero M,Pedranzani H,Zirulnik F,Molina A,PérezChaca MV,Vigliocco A,Abdala G(2011).Short-term cold stress in two cultivars of Digitaria eriantha:effects on stress-related hormones and antioxidant defense system.Acta Physiol Plant 33,497-507.
Hanks RJ(1983).Yield and water-use relationships:an overview.In:Taylor HM,Jordan WR,Sinclair TR,eds.Limitations to Efficient Water Use in Crop Production.Madison:American Society of Agronomy.pp.393-411.
Hille R,Hall J,Basu P(2014).The mononuclear molybdenum enzymes.Chem Rev 114,3963-4038.
Li YJ,Zhang JC,Zhang J,Hao L,Hua JP,Duan LS,Zhang MC,Li ZH(2013).Expression of an Arabidopsis molybdenum cofactor sulphurase gene in soybean enhances drought tolerance and increases yield under field conditions.Plant Biotechnol J 11,747-758.
Lu Y,Li YJ,Zhang JC,Xiao YT,Yue YS,Duan LS,Zhang MC,Li ZH(2013).Overexpression of Arabidopsis molybdenum cofactor sulfurase gene confers drought tolerance in maize(Zea mays L.).PLo S One 8,e52126.
Ma FF,Lu R,Liu HY,Shi B,Zhang JH,Tan MP,Zhang AY,Jiang MY(2012).Nitric oxide-activated calcium/calmodulin-dependent protein kinase regulates the abscisic acid-induced antioxidant defence in maize.J Exp Bot 63,4835-4847.
Mahajan S,Tuteja N(2005).Cold,salinity and drought stresses:an overview.Arch Biochem Biophys 444,139-158.
Patnaitk D,Khurana P(2002).Wheat biotechnology:a minireview.Electron J Biotechnol 4,74-102.
Sun XC,Hu CX,Tan QL,Liu JS,Liu HE(2009).Effects of molybdenum on expression of cold-responsive genes in abscisic acid(ABA)-dependent and ABA-independent pathways in winter wheat under low-temperature stress.Ann Bot 104,345-356.
Wilkinson S,Davies WJ(2002).ABA-based chemical signaling:the co-ordination of responses to stress in plants.Plant Cell Environ 25,195-210.
Wu SW,Hu CX,Tan QL,Nie ZJ,Sun XC(2014).Effects of molybdenum on water utilization,antioxidative defense system and osmotic-adjustment ability in winter wheat(Triticum aestivum)under drought stress.Plant Physiol Biochem 83,365-374.
Xiong LM,Ishitani M,Lee H,Zhu JK(2001).The Arabidopsis LOS5/ABA3 locus encodes a molybdenum cofactor sulfurase and modulates cold stress-and osmotic stress-responsive gene expression.Plant Cell 13,2063-2084.
Xiong LM,Zhu JK(2001).Abiotic stress signal transduction in plants:molecular and genetic perspectives.Physiol Plant 112,152-166.
Yue SY,Ming CZ,Zhang JC,Tian XL,Duan LS,Li ZH(2012).Overexpression of the AtLOS5 gene increased abscisic acid level and drought tolerance in transgenic cotton.J Exp Bot 63,3741-3748.
Yue YS,Zhang MC,Zhang JC,Duan LS,Li ZH(2011).Arabidopsis LOS5/ABA3 overexpression in transgenic tobacco(Nicotiana tabacum cv.‘Xanthi-nc’)results in enhanced drought tolerance.Plant Sci 181,405-411.
Zhang M,Hu CX,Zhao XH,Tan QL,Sun XC,Cao AY,Cui M,Zhang Y(2012).Molybdenum improves antioxidant and osmotic-adjustment ability against salt stress in Chinese cabbage(Brassica campestris L.ssp.pekinensis).Plant Soil 355,375-383.
Zhu JK(2002).Salt and drought stress signal transduction in plants.Annu Rev Plant Biol 53,247-273.