非洲加纳籽乙醇提取物的生物活性研究
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摘要
采用MTT法研究了非洲加纳籽乙醇提取物的抗肝癌活性,并研究了其对DPPH自由基的清除能力。结果表明,加纳籽95%乙醇提取物对肝癌细胞SMMC7721和Bel7402具有较好的抑制活性,IC_(50)值分别为(124.05±11.35)μg·mL~(-1)和(157.05±3.94)μg·mL~(-1);不同浓度乙醇提取的加纳籽乙醇提取物对DPPH自由基的清除能力均明显强于Vc。该研究可为非洲加纳籽的生物活性研究及生物活性成分的分离提供参考。
The anti-hepatoma activities of different concentration of ethanol extracts from Griffonia simplicifolia Baill.were measured by MTT assay,and DPPH free radical scavenging capacity of ethanol extracts fromGriffonia simplicifolia Baill.were studied as well.Results showed that 95%ethanol extract fromGriffonia simplicifolia Baill.had good inhibitory activity on hepatoma cell SMMC7721 and Bel7402,with half-inhibition concentration(IC_(50))of(124.05±11.35)μg·mL~(-1) and(157.05±3.94)μg·mL~(-1),respectively.This study may provide references for bioactivity research and bioactive ingredients separation of Griffonia simplicifolia Baill..
The anti-hepatoma activities of different concentration of ethanol extracts from Griffonia simplicifolia Baill.were measured by MTT assay,and DPPH free radical scavenging capacity of ethanol extracts fromGriffonia simplicifolia Baill.were studied as well.Results showed that 95%ethanol extract fromGriffonia simplicifolia Baill.had good inhibitory activity on hepatoma cell SMMC7721 and Bel7402,with half-inhibition concentration(IC_(50))of(124.05±11.35)μg·mL~(-1) and(157.05±3.94)μg·mL~(-1),respectively.This study may provide references for bioactivity research and bioactive ingredients separation of Griffonia simplicifolia Baill..
引文
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[4]欧艳,肖方青,肖艳萍.5-羟基色氨酸的提取和测定方法研究[J].湖南中医杂志,2005,(4):82-83.
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[6]MOLYNEUX P.The use of the stable free radical diphenylpicrylhydrazyl(DPPH)for estimating antioxidant[J].Songklanakarin Journal of Science&Technology,2004,26(2):211-219.
[7]赵玉英,卢静琼,靳利娥,等.橘皮抗氧化物质的提取及抗氧化性能研究[J].太原理工大学学报,2009,(3):264-267.
[8]LYSEK N,KINSCHERF R,CLAUS R,et al.L-5-Hydroxytryptophan:Antioxidant and anti-apoptotic principle of the intertidal sponge Hymeniacidon heliophila[J].Zeitschrift Fur Naturforschung C A Journal of Biosciences,2003,58(7-8):568-572.
[2]ADDO-FORDJOUR P,ANNING A K,LARBI J A,et al.Liana species richness,abundance and relationship with trees in the Bobiri forest reserve,Ghana:Impact of management systems[J].Forest Ecology&Management,2009,257(8):1822-1828.
[3]CARNEVALE G,di VIESTI V,ZAVATTI M,et al.Anxiolyticlike effect of Griffonia simplicifolia Baill.seed extract in rats[J].Phytomedicine,2011,18(10):848-851.
[4]欧艳,肖方青,肖艳萍.5-羟基色氨酸的提取和测定方法研究[J].湖南中医杂志,2005,(4):82-83.
[5]KEDARE S B,SINGH R P.Genesis and development of DPPH method of antioxidant assay[J].J Food Sci Technol,2011,48(4):412-422.
[6]MOLYNEUX P.The use of the stable free radical diphenylpicrylhydrazyl(DPPH)for estimating antioxidant[J].Songklanakarin Journal of Science&Technology,2004,26(2):211-219.
[7]赵玉英,卢静琼,靳利娥,等.橘皮抗氧化物质的提取及抗氧化性能研究[J].太原理工大学学报,2009,(3):264-267.
[8]LYSEK N,KINSCHERF R,CLAUS R,et al.L-5-Hydroxytryptophan:Antioxidant and anti-apoptotic principle of the intertidal sponge Hymeniacidon heliophila[J].Zeitschrift Fur Naturforschung C A Journal of Biosciences,2003,58(7-8):568-572.