玉米自交系齐319对玉米灰斑病的抗性遗传分析
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摘要
利用齐319与10个不同抗病水平的玉米自交系组配的F_1及其与感病自交系丹340、龙抗11构建的F_2群体为试材,通过人工接种鉴定,研究自交系齐319对灰斑病的抗性遗传规律。结果表明,选用齐319作抗性亲本,由其组配杂交组合可明显降低灰斑病发病程度,除与高感材料组配外,均能使杂交组合抗病性达到中抗以上水平。利用单个分离世代分析,齐319与龙抗11组配的后代群体符合B-2模型,即两对加性-显性主基因遗传模型;与丹340组配后代群体符合B-1模型,即两对加性-显性-上位性主基因遗传模型。利用齐319组配群体,在不同遗传背景下,齐319均表现出2对主效抗性基因,在龙抗11背景下表现出的主基因遗传率更高,基因作用方式更简单。
Qi319 and the level of resistance to the GLS of 10 maize inbred lines were used to produce F_1,and a highly resistance inbred lines Qi319 and susceptible inbred lines Dan340,Longkang11 were used to produce segregating population for inheritance of resistance to gray leaf spot by using artificial inoculation identification method.The results showed that the incidence of gray leaf spot of the hybrid would be significantly reduced when the Qi319 as a resistant parent in addition to cross with highly susceptible inbred lines,the level of resistance to the GLS was increased to the middle level.Segregation analysis of single separate generation was adopted to study the genetic laws of GLS.The result showed that parents Qi319 and Longkang11 were in accordance with the B-2 model,namely two pairs of major genes with additive-dominant effects.The parents Qi319 and Longkang11 were in accordance with the B-1 model,namely two pairs of major genes with additive-dominant-epistatic effects.Qi319 was used to produce population,it could show two pairs of major resistance genes in different genetic background,but Qi319 could show a higher genetic rate of the main gene in the background of the parent Longkang11.
Qi319 and the level of resistance to the GLS of 10 maize inbred lines were used to produce F_1,and a highly resistance inbred lines Qi319 and susceptible inbred lines Dan340,Longkang11 were used to produce segregating population for inheritance of resistance to gray leaf spot by using artificial inoculation identification method.The results showed that the incidence of gray leaf spot of the hybrid would be significantly reduced when the Qi319 as a resistant parent in addition to cross with highly susceptible inbred lines,the level of resistance to the GLS was increased to the middle level.Segregation analysis of single separate generation was adopted to study the genetic laws of GLS.The result showed that parents Qi319 and Longkang11 were in accordance with the B-2 model,namely two pairs of major genes with additive-dominant effects.The parents Qi319 and Longkang11 were in accordance with the B-1 model,namely two pairs of major genes with additive-dominant-epistatic effects.Qi319 was used to produce population,it could show two pairs of major resistance genes in different genetic background,but Qi319 could show a higher genetic rate of the main gene in the background of the parent Longkang11.
引文
[1]Ward J M J,Stromberg E L,Nowell D C,et al.Gray leaf spot:A disease of global importance in maize production[J].Plant Disease,1999,83:884-895.
[2]Tehon L R,Daniels E.Notes on the parasitic fungi of Illinois[J].Mycologia,1925,17:240-249.
[3]Crous P W,Groenewald J Z,Groenewald M,et al.Species of Cercospora associated with grey leaf spot of maize[J].Stud Mycol,2006:189-197.
[4]赵立萍,王晓鸣,段灿星,等.中国玉米灰斑病发生现状与未来扩散趋势分析[J].中国农业科学,2015(18):3612-3626.Zhao L P,Wang X M,Duan C X,et al.Occurrence status and future spreading areas of maize gray leaf spot in China[J].Scientia Agricultura Sinica,2015(18):3612-3626.(in Chinese)
[5]刘庆奎,秦子惠,张小利,等.中国玉米灰斑病病原菌的鉴定及其基本特征研究[J].中国农业科学,2013,46(19):4044-4057.Liu Q K,Qin Z H,Zhang X L,et al.Identification of Cercospora species associated with maize gray leaf spot in China[J].Scientia Agricultura Sinica,2013,46(19):4044-4057.(in Chinese)
[6]Ward J M J,Nowell D C.Integrated management practices for the control of maize grey leaf spot[J].Integrated Pest Management Reviews,1998,33:177-188.
[7]吴纪昌,马丽君,孙义,等.玉米一种新病害—尾孢菌叶斑病(Cercospora zeae-maydis)大发生[J].玉米科学,1992(1):67-68.Wu J C,Ma L J,Sun Y,et al.A new disease of maize-Cercospora zeae-maydis[J].Journal of Maize Sciences,1992(1):67-68.(in Chinese)
[8]吴纪昌,马丽君,王作英.玉米抗尾孢菌叶斑病鉴定与抗病材料利用[J].辽宁农业科学,1997(5):25-28.Wu J C,Ma L J,Wang Z Y,Identification of maize against Cercospora leaf spot disease and utilization of disease resistance materials[J].Liaoning Agricultural Sciences,1997(5):25-28.(in Chinese)
[9]苏前富,张伟,宋淑云,等.2007年吉林省玉米主要病害调查及其发生趋势预测[J].玉米科学,2008,16(5):135-137.Su Q F,Zhang W,Song S Y,et al.Research and forecast of catastrophic tendency of the main corn diseases in Jilin province in 2007[J].Journal of Maize Sciences,2008,16(5):135-137.(in Chinese)
[10]马莹莹,贾娇,苏前富,等.玉米抵御玉蜀黍尾孢菌侵入的生理机制[J].植物保护学报,2015,42(3):340-346.Ma Y Y,Jia J,Su Q F,et al.The physiological mechanism of corn against Cercospora zeae-maydis infection[J].Journal of Plant Protection,2015,42(3):340-346.(in Chinese)
[11]Coates S T,White D G.Inheritance of resistance to gray leaf spot in crosses involving selected resistant inbred lines of corn[J].Phytopathology,2008,889:972-982.
[12]吕国忠,张益先,梁景颐,等.玉米灰斑病发生流行规律及品种抗病性[J].植物病理学报,2003,33(5):462-467.LüG Z,Zhang Y X,Liang J Y,et al.Epidemics of gray leaf spot of corn(Cercospora zeae-maydis)and varietal resistance[J].Acta Phytopathologica Sinica,2003,33(5):462-467.(in Chinese)
[13]董怀玉,姜钰,王丽娟,等.玉米种质资源抗灰斑病鉴定与评价[J].植物遗传资源学报,2005,6(4):441-443.Dong H Y,Jiang Y,Wang L J,et al.Evaluation on maize germplasm resources for resistance to gray leaf spot[J].Journal on Plant Genetic Resources,2005,6(4):441-443.(in Chinese)
[14]Abebe Menkir.Genetic analysis of resistance to cray leaf spot of midaltitude maize inbred lines[J].Crop Science,2005,45(1):163-170.
[15]Latterell F M,Rossi A E.Gray leaf spot of corn:A disease on the move[J].Plant Disease,1983,67.
[16]吕香玲,李新海,陈阳,等.玉米种质抗灰斑病鉴定与评价[J].玉米科学,2011,19(6):125-128.LüX L,Li X H,Chen Y,et al.Evaluation and identification of resistance to gray leaf spot in maize germplasm[J].Journal of Maize Sciences,2011,19(6):125-128.(in Chinese)
[17]佟淑杰,徐秀德,董怀玉,等.玉米灰斑病菌产孢特性的初步研究[J].杂粮作物,2005,25(3):201-203.Tong S J,Xu X D,Dong H Y,et al.The study of the characteristics of conidial production to gray leaf spot of maize[J].Rain Fed Crops,2005,25(3):201-203.(in Chinese)
[18]盖钧镒.植物数量性状遗传体系的分离分析方法研究[J].遗传,2005,27(1):130-136.Gai J Y.Segregation analysis of genetic system of quantitative traits in plants[J].Hereditas,2005,27(1):130-136.(in Chinese)
[19]Xie C,Zhang S,Li M,et al.Inferring genome ancestry and estimating molecular relatedness among 187 Chinese maize inbred lines[J].Journal of Genetics&Genomics,2007,34(8):738-748.
[20]王建康,盖钧镒.利用杂种F2世代鉴定数量性状主基因-多基因混合遗传模型并估计其遗传效应[J].遗传学报,1997,24(5):432-440.Wang J K,Gai J Y.Identification of major gene and polygene mixed inheritance model and estimation genetic parameters of a quantitative trait from F2progeny[J].Acta Genetica Sinica,1997,24(5):432-440.(in Chinese)
[21]徐秀德,董怀玉,姜钰,等.玉米灰斑病抗性鉴定技术[J].植物保护学报,2003,30(2):129-132.Xu X D,Dong H Y,Jiang Y,et al.The technique for identification of resistance to gray leaf spot on maize[J].Journal of Plant Protection,2003,30(2):129-132.(in Chinese)
[22]叶金才.育成我国首例对玉米南方锈病免疫系齐319[J].中国农业科学,2000,33(4):110-110.Ye C J.The first inbred lines Qi319 immune to maize southern rust bred in China[J].Scientia Agricultura Sinica,2000,33(4):110-110.(in Chinese)
[23]刘翠娜,代君丽,吴刘记,等.玉米自交系齐319对弯孢霉叶斑病抗性的遗传分析[J].河南农业大学学报,2015,49(2):234-238.Liu C N,Dai J L,Wu L J,et al.Genetic analysis of resistance to Curvularia leaf spot of maize(Zea mays L.)inbred line Qi319[J].Journal of Henan Agricultural University,2015,49(2):234-238.(in Chinese)
[24]宋凤景,肖明纲,李洪杰,等.玉米自交系齐319抗腐霉茎腐病基因的遗传分析和分子定位[C].中国植物保护学会学术年会,2014.
[25]陈翠霞,杨典洱,王振林,等.齐319携带的南方玉米锈病抗性基因的遗传初析[J].遗传学报,2002,29(10):903-906.Chen C X,Yang D E,Wang Z L,et al.Inheritance of resistance to southern corn rust in the inbred line Qi319[J].Journal of Genetics and Genomics,2002,29(10):903-906.(in Chinese)
[26]Lehmensiek A,Esterhuizen A M,van Staden D,et al.Genetic mapping of gray leaf spot(GLS)resistance genes in maize[J].Theor Appl Genet,2001,103:797-803.
[27]Gordon S G,Bartsch M,Matthies I,et al.Linkage of molecular markers to Cercosporazeae-maydis resistance in maize[J].Crop Sci.,2004,44:628-636.
[28]Zhang Y,Xu L,Fan X M,et al.QTL mapping of resistance to gray leaf spot in maize[J].Theor Appl Genet,2012,125:1797-1808.
[2]Tehon L R,Daniels E.Notes on the parasitic fungi of Illinois[J].Mycologia,1925,17:240-249.
[3]Crous P W,Groenewald J Z,Groenewald M,et al.Species of Cercospora associated with grey leaf spot of maize[J].Stud Mycol,2006:189-197.
[4]赵立萍,王晓鸣,段灿星,等.中国玉米灰斑病发生现状与未来扩散趋势分析[J].中国农业科学,2015(18):3612-3626.Zhao L P,Wang X M,Duan C X,et al.Occurrence status and future spreading areas of maize gray leaf spot in China[J].Scientia Agricultura Sinica,2015(18):3612-3626.(in Chinese)
[5]刘庆奎,秦子惠,张小利,等.中国玉米灰斑病病原菌的鉴定及其基本特征研究[J].中国农业科学,2013,46(19):4044-4057.Liu Q K,Qin Z H,Zhang X L,et al.Identification of Cercospora species associated with maize gray leaf spot in China[J].Scientia Agricultura Sinica,2013,46(19):4044-4057.(in Chinese)
[6]Ward J M J,Nowell D C.Integrated management practices for the control of maize grey leaf spot[J].Integrated Pest Management Reviews,1998,33:177-188.
[7]吴纪昌,马丽君,孙义,等.玉米一种新病害—尾孢菌叶斑病(Cercospora zeae-maydis)大发生[J].玉米科学,1992(1):67-68.Wu J C,Ma L J,Sun Y,et al.A new disease of maize-Cercospora zeae-maydis[J].Journal of Maize Sciences,1992(1):67-68.(in Chinese)
[8]吴纪昌,马丽君,王作英.玉米抗尾孢菌叶斑病鉴定与抗病材料利用[J].辽宁农业科学,1997(5):25-28.Wu J C,Ma L J,Wang Z Y,Identification of maize against Cercospora leaf spot disease and utilization of disease resistance materials[J].Liaoning Agricultural Sciences,1997(5):25-28.(in Chinese)
[9]苏前富,张伟,宋淑云,等.2007年吉林省玉米主要病害调查及其发生趋势预测[J].玉米科学,2008,16(5):135-137.Su Q F,Zhang W,Song S Y,et al.Research and forecast of catastrophic tendency of the main corn diseases in Jilin province in 2007[J].Journal of Maize Sciences,2008,16(5):135-137.(in Chinese)
[10]马莹莹,贾娇,苏前富,等.玉米抵御玉蜀黍尾孢菌侵入的生理机制[J].植物保护学报,2015,42(3):340-346.Ma Y Y,Jia J,Su Q F,et al.The physiological mechanism of corn against Cercospora zeae-maydis infection[J].Journal of Plant Protection,2015,42(3):340-346.(in Chinese)
[11]Coates S T,White D G.Inheritance of resistance to gray leaf spot in crosses involving selected resistant inbred lines of corn[J].Phytopathology,2008,889:972-982.
[12]吕国忠,张益先,梁景颐,等.玉米灰斑病发生流行规律及品种抗病性[J].植物病理学报,2003,33(5):462-467.LüG Z,Zhang Y X,Liang J Y,et al.Epidemics of gray leaf spot of corn(Cercospora zeae-maydis)and varietal resistance[J].Acta Phytopathologica Sinica,2003,33(5):462-467.(in Chinese)
[13]董怀玉,姜钰,王丽娟,等.玉米种质资源抗灰斑病鉴定与评价[J].植物遗传资源学报,2005,6(4):441-443.Dong H Y,Jiang Y,Wang L J,et al.Evaluation on maize germplasm resources for resistance to gray leaf spot[J].Journal on Plant Genetic Resources,2005,6(4):441-443.(in Chinese)
[14]Abebe Menkir.Genetic analysis of resistance to cray leaf spot of midaltitude maize inbred lines[J].Crop Science,2005,45(1):163-170.
[15]Latterell F M,Rossi A E.Gray leaf spot of corn:A disease on the move[J].Plant Disease,1983,67.
[16]吕香玲,李新海,陈阳,等.玉米种质抗灰斑病鉴定与评价[J].玉米科学,2011,19(6):125-128.LüX L,Li X H,Chen Y,et al.Evaluation and identification of resistance to gray leaf spot in maize germplasm[J].Journal of Maize Sciences,2011,19(6):125-128.(in Chinese)
[17]佟淑杰,徐秀德,董怀玉,等.玉米灰斑病菌产孢特性的初步研究[J].杂粮作物,2005,25(3):201-203.Tong S J,Xu X D,Dong H Y,et al.The study of the characteristics of conidial production to gray leaf spot of maize[J].Rain Fed Crops,2005,25(3):201-203.(in Chinese)
[18]盖钧镒.植物数量性状遗传体系的分离分析方法研究[J].遗传,2005,27(1):130-136.Gai J Y.Segregation analysis of genetic system of quantitative traits in plants[J].Hereditas,2005,27(1):130-136.(in Chinese)
[19]Xie C,Zhang S,Li M,et al.Inferring genome ancestry and estimating molecular relatedness among 187 Chinese maize inbred lines[J].Journal of Genetics&Genomics,2007,34(8):738-748.
[20]王建康,盖钧镒.利用杂种F2世代鉴定数量性状主基因-多基因混合遗传模型并估计其遗传效应[J].遗传学报,1997,24(5):432-440.Wang J K,Gai J Y.Identification of major gene and polygene mixed inheritance model and estimation genetic parameters of a quantitative trait from F2progeny[J].Acta Genetica Sinica,1997,24(5):432-440.(in Chinese)
[21]徐秀德,董怀玉,姜钰,等.玉米灰斑病抗性鉴定技术[J].植物保护学报,2003,30(2):129-132.Xu X D,Dong H Y,Jiang Y,et al.The technique for identification of resistance to gray leaf spot on maize[J].Journal of Plant Protection,2003,30(2):129-132.(in Chinese)
[22]叶金才.育成我国首例对玉米南方锈病免疫系齐319[J].中国农业科学,2000,33(4):110-110.Ye C J.The first inbred lines Qi319 immune to maize southern rust bred in China[J].Scientia Agricultura Sinica,2000,33(4):110-110.(in Chinese)
[23]刘翠娜,代君丽,吴刘记,等.玉米自交系齐319对弯孢霉叶斑病抗性的遗传分析[J].河南农业大学学报,2015,49(2):234-238.Liu C N,Dai J L,Wu L J,et al.Genetic analysis of resistance to Curvularia leaf spot of maize(Zea mays L.)inbred line Qi319[J].Journal of Henan Agricultural University,2015,49(2):234-238.(in Chinese)
[24]宋凤景,肖明纲,李洪杰,等.玉米自交系齐319抗腐霉茎腐病基因的遗传分析和分子定位[C].中国植物保护学会学术年会,2014.
[25]陈翠霞,杨典洱,王振林,等.齐319携带的南方玉米锈病抗性基因的遗传初析[J].遗传学报,2002,29(10):903-906.Chen C X,Yang D E,Wang Z L,et al.Inheritance of resistance to southern corn rust in the inbred line Qi319[J].Journal of Genetics and Genomics,2002,29(10):903-906.(in Chinese)
[26]Lehmensiek A,Esterhuizen A M,van Staden D,et al.Genetic mapping of gray leaf spot(GLS)resistance genes in maize[J].Theor Appl Genet,2001,103:797-803.
[27]Gordon S G,Bartsch M,Matthies I,et al.Linkage of molecular markers to Cercosporazeae-maydis resistance in maize[J].Crop Sci.,2004,44:628-636.
[28]Zhang Y,Xu L,Fan X M,et al.QTL mapping of resistance to gray leaf spot in maize[J].Theor Appl Genet,2012,125:1797-1808.