HPLC测定排毒养颜胶囊中番泻苷A、番泻苷B含量
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摘要
目的:建立测定排毒养颜胶囊中番泻苷A、番泻苷B含量的方法。方法:采用Sepax-C_(18)柱(5μm,4.6mm×250mm),以乙腈-5mmol/L四庚基溴化铵的醋酸-醋酸钠缓冲液(pH=5.0)(15∶85)为流动相,流速1.0mL/min;检测波长340nm;柱温35℃。结果:番泻苷A和番泻苷B在各自的线性范围内均呈良好的线性关系(R~2=1),平均加样回收率分别为100.90%、101.37%,RSD为1.62%、1.08%。结论:该实验建立的方法专属性强,操作简便,可用于排毒养颜胶囊的质量控制。
Objective:To establish a method for determining the content of Sennoside A and sennoside B in PaiduYangyan capsules.Methods:The determination was performed on Sepax-C_(18) column with mobile phase consisted of acetonitrile-5 mmol/L tetraheptylammonium bromide acetate-sodium acetate buffer(pH=5.0)(15:85),flow rate is 1.0 mL/min;detection wavelength is 340 nm;column temperature is 35°C.Results:Sennoside A and sennoside B have a good linear relationship in their linear range(R~2=1).The average recovery rate was 100.90%,101.37%;RSD values are 1.62%,1.08%.Conclusion:The method established in this experiment is specific and easy to operate,can be used for the quality control of Paiduyangyan capsules.
Objective:To establish a method for determining the content of Sennoside A and sennoside B in PaiduYangyan capsules.Methods:The determination was performed on Sepax-C_(18) column with mobile phase consisted of acetonitrile-5 mmol/L tetraheptylammonium bromide acetate-sodium acetate buffer(pH=5.0)(15:85),flow rate is 1.0 mL/min;detection wavelength is 340 nm;column temperature is 35°C.Results:Sennoside A and sennoside B have a good linear relationship in their linear range(R~2=1).The average recovery rate was 100.90%,101.37%;RSD values are 1.62%,1.08%.Conclusion:The method established in this experiment is specific and easy to operate,can be used for the quality control of Paiduyangyan capsules.
引文
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[5]张依倩,王玉,黄芝娟,等.基于HPLC-DAD-MS的道地产区大黄药材质量评价研究[J].药物评价研究,2011,34(3):179-183.
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[7]吕达明,刘传岸,李胤嘉,等.大黄牡公灌肠散质量标准研究[J].中成药,2014,36(9):1876-1881.
[8]刘月红,黄政海,董玲,等.高效液相色谱法同时测定大黄中14种成分的含量[J].中国中药杂志,2017(23):4514-4519.
[9]陈应奇,唐宋琪,刑春蕊,等.莫家清宁丸中7个成分的HPLC-波长切换法测定[J].中国医药工业杂志,2017,48(2):239-242.
[10]陈丽萍,李茂星,马幸福.HPLC同时测定唐古特大黄地上部分6种化学成分含量[J].中国中医药信息杂志,2017,24(10):76-80.
[2]马蓉,张雪菊.液相色谱法对不同产地大黄中番泻苷A含量比较分析[J].中成药,2008,30(10):1489-1490.
[3]王家葵,李傲,王慧,等.正品大黄不同品种间泻下效价强度比较研究[J].中国中药杂志,2006,31(23):1987-1991.
[4]谭鹏,张海珠.大黄趁鲜加工工艺:定尺寸饮片的研制及其质量评价[J].中草药,2017,48(12):2407-2414.
[5]张依倩,王玉,黄芝娟,等.基于HPLC-DAD-MS的道地产区大黄药材质量评价研究[J].药物评价研究,2011,34(3):179-183.
[6]国家药典委员会.中华人民共和国药典[M].一部.北京:中国医药科技出版社,2015:347-348.
[7]吕达明,刘传岸,李胤嘉,等.大黄牡公灌肠散质量标准研究[J].中成药,2014,36(9):1876-1881.
[8]刘月红,黄政海,董玲,等.高效液相色谱法同时测定大黄中14种成分的含量[J].中国中药杂志,2017(23):4514-4519.
[9]陈应奇,唐宋琪,刑春蕊,等.莫家清宁丸中7个成分的HPLC-波长切换法测定[J].中国医药工业杂志,2017,48(2):239-242.
[10]陈丽萍,李茂星,马幸福.HPLC同时测定唐古特大黄地上部分6种化学成分含量[J].中国中医药信息杂志,2017,24(10):76-80.