热应激对西南地区肉兔生理生化指标的影响
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摘要
为了研究西南地区夏季热应激对肉兔生理生化指标的影响,本试验选择8只健康肉兔,分别测定非热应激期和热应激期兔舍环境温度和相对湿度,并测定试验肉兔血清中生理生化指标。结果表明:(1)热应激期试验肉兔的直肠温度、耳根温度、尾根温度和呼吸频率均极显著高于非热应激期(P<0.01),颈部温度显著高于非热应激期(P<0.05);(2)与非热应激期相比,热应激期肉兔血清中三碘甲状腺原氨酸(T_3)含量差异不显著(P>0.05),甲状腺素(T_4)含量在第30天显著降低(P<0.05),皮质醇(COR)含量极显著增加(P<0.01),热休克蛋白70(HSP70)含量显著增加(P<0.05),丙二醛(MDA)含量在第1天升高而后逐渐恢复正常(P>0.05)。这表明夏季高温高湿环境诱发了肉兔的热应激反应,热应激肉兔表现为直肠温度、耳根温度、颈部温度、尾根温度及呼吸频率升高,内分泌系统紊乱,抗氧化能力降低。
This study is conducted to investigate the effects of summer heat stress on physiological and serum biochemical indices of beef cattle in southwest China.Eight meat rabbit were fed in normal and heat stress conditions.During the trials,temperature and relative humidity of rabbit house were determined.The serum samples of meat rabbit were collected,and then physiological and serum biochemical indices of meat rabbit were determined.The results show as follows:(1)rectal temperature,ear root temperature,tail root temperature and breathing frequency of meat rabbit in heat stress are extremely higher than those in normal period(P<0.01),neck temperature is significantly higher than those in normal period(P<0.05);(2)Compared with normal period,triiodothyronine(T3)content in serum is not changed(P>0.05),but thyroxine(T4)content is significantly lower on the 30 th day(P<0.05),cortisol(COR)content is extremely increased(P<0.01),heat shock protein 70(HSP70)content is significantly increased(P<0.05),malondialdehyde(MDA)content is increased on the first day and then gradually recovered to normal(P>0.05).These results indicate that high temperature and high humidity induce heat stress of meat rabbit,and heat stress can enhance the rectal temperature,ear root temperature,neck temperature,tail root temperature and breathing frequency,disorder endocrine and reduce antioxidant ability.
This study is conducted to investigate the effects of summer heat stress on physiological and serum biochemical indices of beef cattle in southwest China.Eight meat rabbit were fed in normal and heat stress conditions.During the trials,temperature and relative humidity of rabbit house were determined.The serum samples of meat rabbit were collected,and then physiological and serum biochemical indices of meat rabbit were determined.The results show as follows:(1)rectal temperature,ear root temperature,tail root temperature and breathing frequency of meat rabbit in heat stress are extremely higher than those in normal period(P<0.01),neck temperature is significantly higher than those in normal period(P<0.05);(2)Compared with normal period,triiodothyronine(T3)content in serum is not changed(P>0.05),but thyroxine(T4)content is significantly lower on the 30 th day(P<0.05),cortisol(COR)content is extremely increased(P<0.01),heat shock protein 70(HSP70)content is significantly increased(P<0.05),malondialdehyde(MDA)content is increased on the first day and then gradually recovered to normal(P>0.05).These results indicate that high temperature and high humidity induce heat stress of meat rabbit,and heat stress can enhance the rectal temperature,ear root temperature,neck temperature,tail root temperature and breathing frequency,disorder endocrine and reduce antioxidant ability.
引文
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[12]张瑞光,吴晓鸣,鲍若虹,等.热应激与非热应激期福安水牛生理和抗氧化指标分析[J].江西农业大学学报,2010,22(11):136-138.
[13]王文娟,汪水平,左福元,等.中药复方对夏季肉牛的影响:生产性能、生理指标及血清激素水平和酶活性[J].畜牧兽医学报,2010,41(10):1 260-1 267.
[14]黄桂花,谢梅冬,梁明振,等.热应激对公兔繁殖性能及血清生化指标的影响[J].现代农业科技,2010(6):321-322.
[15]何钦.热应激对不同泌乳阶段奶牛生产性能及其营养代谢的影响[D].重庆:西南大学,2012.
[16]李朝明.荷斯坦奶牛、娟姗牛和娟荷杂交牛热适应性的研究[D].重庆:西南大学,2013.
[17]ANGELIDIS C E,LAZARIDIS I,PAGOULATOS G N.Constitutive expression of heat-shock protein 70in mammalian cells confers thermoresistance[J].Eur J Biochem,1991,199(1):35-39.
[18]肖正中,周晓情,黄光云,等.不同添加剂对娟姗奶牛产奶性能及血清HSP70的影响[J].黑龙江畜牧兽医,2016(14):63-65.
[19]王艳萍,祁克宗,涂健,等.内毒素对仔鸡血浆SOD活性及MDA水平的影响[J].动物医学进展,2006,27(5):63-65.
[2]PAPP J G,SZELERES L.Short incubation with 7-0x0-prostacyclin induces long lasting prolongation of repolarization time and effective refractory period in rabbit papillary muscle preparation[J].European Society of Cardiology,1990,24(1):37-41.
[3]LINDQUIST S.The heat-shock response[J].Annual Review of Biochemistry,1986(55):1 151-1 191.
[4]朱长彬.家兔热应激及其缓解措施[J].山东畜牧兽医,2010(8):48-49.
[5]杨丽萍,高淑霞,白莉雅,等.不同季节长毛兔产毛性能比较研究[J].中国养兔,2016(5):4-6.
[6]张立永,官丽辉.大豆异黄酮对种公兔睾丸指数和某些激素含量的影响[J].中国兽医学报,2015,35(1):155-158.
[7]BAHGA C S,KAUR P,HANDA M C.Performance of meat and wool type rabbit as affected by heat stress and microclimatic modification[J].Indian Journal of Animal Research,2010(1):67-69.
[8]王自力,张翥,唐琪,等.中药复方对热应激肉兔肠道组织结构及肝脏抗氧化机能的影响[J].中国兽医杂志,2014,50(6):48-51,54.
[9]MARAI I F M,AYYAT M S,EL-MONEM U M A.Growth performance and reproductive traits at first parity of New Zealand white female rabbits as affected by heat stress and its alleviation under Egyptian conditions[J].Tropical Animal Health and Production,2001,33(6):451-462.
[10]姚勤,安代志,颜培实.温热环境与奶牛的生理调节特性[J].安徽农业科学,2006,34(6):1 089-1 090.
[11]李超,赵青余,马悦培,等.γ-氨基丁酸对持续高温热应激肉仔鸡生产性能和血液指标的影响[J].中国兽医学报,2010,30(4):556-559.
[12]张瑞光,吴晓鸣,鲍若虹,等.热应激与非热应激期福安水牛生理和抗氧化指标分析[J].江西农业大学学报,2010,22(11):136-138.
[13]王文娟,汪水平,左福元,等.中药复方对夏季肉牛的影响:生产性能、生理指标及血清激素水平和酶活性[J].畜牧兽医学报,2010,41(10):1 260-1 267.
[14]黄桂花,谢梅冬,梁明振,等.热应激对公兔繁殖性能及血清生化指标的影响[J].现代农业科技,2010(6):321-322.
[15]何钦.热应激对不同泌乳阶段奶牛生产性能及其营养代谢的影响[D].重庆:西南大学,2012.
[16]李朝明.荷斯坦奶牛、娟姗牛和娟荷杂交牛热适应性的研究[D].重庆:西南大学,2013.
[17]ANGELIDIS C E,LAZARIDIS I,PAGOULATOS G N.Constitutive expression of heat-shock protein 70in mammalian cells confers thermoresistance[J].Eur J Biochem,1991,199(1):35-39.
[18]肖正中,周晓情,黄光云,等.不同添加剂对娟姗奶牛产奶性能及血清HSP70的影响[J].黑龙江畜牧兽医,2016(14):63-65.
[19]王艳萍,祁克宗,涂健,等.内毒素对仔鸡血浆SOD活性及MDA水平的影响[J].动物医学进展,2006,27(5):63-65.