烟株发育过程中硝酸盐积累与调控的研究进展
详细信息    查看全文 | 推荐本文 |
  • 英文篇名:Research progress in nitrate accumulation and control in tobacco leaves in growing period
  • 作者:冯雨晴 ; 杨惠娟 ; 史宏志
  • 英文作者:FENG Yuqing;YANG Huijuan;SHI Hongzhi;National Tobacco Cultivation & Physiology & Biochemistry Research Center, Tobacco Harm Reduction Research Center,Henan Agricultural University;
  • 关键词:烟草 ; 硝酸盐 ; 烟草特有亚硝胺 ; 积累与调控
  • 英文关键词:tobacco;;nitrate;;tobacco specific nitrosamines;;accumulation and regulation
  • 中文刊名:ZGYB
  • 英文刊名:Acta Tabacaria Sinica
  • 机构:河南农业大学烟草学院/烟草行业烟草栽培重点实验室/烟草农业减害研究中心;
  • 出版日期:2019-04-13 09:36
  • 出版单位:中国烟草学报
  • 年:2019
  • 期:v.25
  • 基金:烟草行业烟草栽培重点实验室项目“烟草硝酸盐和生物碱含量对烟叶贮藏过程中TSNA形成的影响”(yczp201504)
  • 语种:中文;
  • 页:ZGYB201902018
  • 页数:12
  • CN:02
  • ISSN:11-2985/TS
  • 分类号:113-124
摘要
烟草中的硝酸盐是有害物质烟草特有亚硝胺(TSNA)的前体物,烟叶叶片和主脉中硝态氮含量与烟叶调制和贮藏过程中TSNA形成密切相关,降低硝酸盐的积累是降低烟草TSNA和提高氮素利用率的重要途径,对优质低害高效烟叶生产具有重要意义。本文综述了烟叶硝酸盐积累机理与影响因素以及调控措施,并对下一步的研究和应用进行了展望,以期为烟草优质低害研究提供借鉴与参考。
        Nitrate is a precursor of tobacco specific nitrosamines(TSNA). The content of nitrate nitrogen in tobacco lamina and midrib is closely related to the formation of TSNA in curing and storage stage. Reducing nitrate accumulation is an important way to reduce content of TSNA in tobacco and improve nitrogen utilization, which is of great significance to the production of high quality, low harm and high efficiency tobacco leaves. In this review, the mechanism of nitrate accumulation, factors of nitrate accumulation and regulation techniques were summarized. In addition, this paper presents an outlook of future research and application in the hope of providing reference for further study on production of high quality and less harmful tobacco leaves.
引文
[1]黄彩变.叶菜硝态氮吸收、溢泌及还原转化与品种间叶柄硝态氮积累差异的关系[D].杨凌:西北农林科技大学, 2009.HUANGCaibian.Nitrateuptake,efflux,reductionand transformation, and their relationships to petiole nitrate ac cumulation over leafy vegetable cultivars[D].Yangling:Northweat A&F University, 2009.
    [2]都韶婷,章永松,林咸永,等.蔬菜积累的硝酸盐及其对人体健康的影响[J].中国农业科学, 2007(09):2007-2014.DU Shao ting, ZHANG Yong song, LIN Xian yong, et al.Accumulation of nitrate in vegetables and its possible implications to human health[J]. Scientia Agricultura Sinica, 2007(09):2007-2014.
    [3]Speijers G J A. Nitrate, toxicological evaluation of certain food additives and contaminants in food. WHO Food Additives Series.Geneva:World Health Organization. 1996, 35:325-360.
    [4]Santamaria P. Nitrate in vegetables:toxicity, content, intake and EC regulation. A review[J]. Journal of the science of food and agriculture, 2006, 86:10-17.
    [5]Hong-Zhi S H I. The relationships between TSNAs and their precursors in burley tobacco from different regions and varieties[J].Acta Tabacaria Sinica, 2012, 18(5):0-0.
    [6]曾凡海,李卫,周冀衡,等.烟草特有亚硝胺(TSNA)的研究进展[J].中国农学通报, 2010, 26(10):82-86.ZENG Fanhai, LI Wei, ZHOU Jiheng, et al. Advances of Tobaccospecific Nitrosamines(TSNA)in tobacco[J]. Chinese Agricultural Science Bulletin, 2010, 26(10):82-86.
    [7]Liang S, Yang J, Zhou J, et al. Application of exogenous substances reduces tobacco-specific nitrosamines content by regulating biosynthesis of nicotine and nitrite in burley tobacco[J]. Acta physiologiae plantarum, 2013, 35(10):3027-3036.
    [8]王瑞云.烟草贮藏过程中TSNA形成及与温度和硝酸盐含量的关系[D].河南农业大学, 2014.WANG Ruiyun. Formation of TSNA during tobacco storage and the relationship with temperature and nitrate contents[D].Zhengzhou:Henan Agricultural University,2014.
    [9]Hoffmann D, Dong M, Hecht S S. Origin in tobacco smoke of N'-nitrosonornicotine, a tobacco-specific carcinogen:brief communication[J]. Journal of the National Cancer Institute, 1977,58(6):1841-1844.
    [10]El-Bayoumy K, Iatropoulos M, Amin S, et al. Increased expression of cyclooxygenase-2 in rat lung tumors induced by the tobaccospecific nitrosamine 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone:the impact of a high-fat diet[J]. Cancer Research, 1999,59(7):1400-1403.
    [11]Wang G, Wang L, Bhoopalan V, et al. The role of XPC protein deficiency in tobacco smoke-induced DNA hypermethylation of tumor suppressor genes[J]. Open Journal of Genetics, 2013, 3(4):285.
    [12]Goepfert S., Bovet L., Lu J., et al. Downregulation of a putative nitrate transporter gene substantially reduces the accumulation of TSNAs in air-crued tobaccos. CORESTA 2018 Congress, Yunnan,China(abstrat AP24).
    [13]陈翔,周开绪,杨兴友,等.不同栽培措施对白肋烟TSNAs及其前体物的影响[J].中国烟草科学, 2017, 38(2):14-18.CHEN Xiang, ZHOU Kaixu, YANG Xingyou, et al. Effects of different cultivation measures on TSNAs and their precursors in burley tobacco[J]. Chinese Tobacco Science, 2017, 38(2):14-18.
    [14]邹颉,余婧,付强,等.烟碱去甲基化酶及降烟碱相关遗传改良研究进展[J].湖南农业科学, 2013(17):5-8.ZOUJie, YUJing,FUQiang,etal. Advancesingenetic modification related to removing methylase from nicotine and nicotine reducing[J]. Hunan Agricultural Sciences, 2013(17):5-8.
    [15]朱风鹏,赵明月,胡清源,等.TSNAs的形成、影响因素、分析及清除方法综述[J].烟草科技, 2004(07):27-30.ZHU Fengpeng, ZHAO Mingyue, HU Qingyuan, et al. Summary on formation, influencing factors, analysis and scavenging of Tobaccospecific Nitrosamines[J]. Tobacco Science, 2004(07):27-30.
    [16]马珂.低氮诱导表达基因、氮转运基因的功能研究以及水稻OsRHC基因家族分析[D].华中农业大学, 2009.MA Ke. Functional analysis of nitrogen-induced genesnitrogen absorption genes and analysis of OsRHC gene family in rice[D].Huazhong Agricultural University, 2009.
    [17]贾宏昉,张洪映,刘维智,等. 2014.高等植物硝酸盐转运蛋白的功能及其调控机制.生物技术通报, 06:14-21.JIA Hongfang, ZHANG Hongying,LIU Weizhi, et al. Function and regulation mechanisms of nitrate transporters in higher plants.BiotechnologyI Bulletin, 06:14-21.
    [18]Forde B G. Nitrate transporters in plants:structure, function and regulation[J]. Biochimica et Biophysica Acta(BBA)-Biomembranes, 2000, 1465(1-2):219-235.
    [19]Moir J W B, Wood N J. Nitrate and nitrite transport in bacteria[J].Cellular and Molecular Life Sciences CMLS, 2001, 58(2):215-224.
    [20]Wang Y Y, Hsu P K, Tsay Y F. Uptake, allocation and signaling of nitrate[J]. Trends in plant science, 2012, 17(8):458-467.
    [21]胡廷章,何帅,黄小云,等.植物中硝酸盐转运蛋白的运输和信号传输功能[J].植物生理学通讯, 2009, 45(11):1131-1136.HU Tingzhang, HE Shuai, HUANG Xiaoyun, et al. Functions on nitrate transportation and signal transduction of nitrate transporter in plants[J]. Plant Physiology Journal, 2009, 45(11):1131-1136.
    [22]Li W, Wang Y, Okamoto M, et al. Dissection of the AtNRT2.1:AtNRT2. 2 inducible high-affinity nitrate transporter gene cluster[J]. Plant physiology, 2007, 143(1):425-433.
    [23]Tsay Y F, Schroeder J I, Feldmann K A, et al. The herbicide sensitivity gene CHL1 of Arabidopsis encodes a nitrate-inducible nitrate transporter[J]. Cell, 1993, 72(5):705-713.
    [24]Chiu C C, Lin C S, Hsia A P, et al. Mutation of a nitrate transporter,AtNRT1:4, results in a reduced petiole nitrate content and altered leaf development[J]. Plant and Cell Physiology, 2004, 45(9):1139-1148.
    [25]Lin S H, Kuo H F, Canivenc G, et al. Mutation of the Arabidopsis NRT1. 5 nitrate transporter causes defective root-to-shoot nitrate transport[J]. The Plant Cell, 2008, 20(9):2514-2528.
    [26]Fan S C, Lin C S, Hsu P K, et al. The Arabidopsis nitrate transporter NRT1. 7, expressed in phloem, is responsible for source-to-sink remobilization of nitrate[J]. The Plant Cell, 2009, 21(9):2750-2761.
    [27]Guo F Q, Wang R, Crawford N M. The Arabidopsis dual‐affinity nitrate transporter gene AtNRT1. 1(CHL1)is regulated by auxin in both shoots and roots[J]. Journal of Experimental Botany, 2002,53(370):835-844.
    [28]Hu B, Wang W, Ou S, et al. Variation in NRT1. 1B contributes to nitrate-use divergence between rice subspecies[J]. Nature Genetics,2015, 47(7):834.
    [29]刘维智.烤烟碳氮代谢对氮素形态的响应及NRT2.2的克隆[D].河南农业大学,2014.LIU Weizhi. Carbon and nitrogen metabolism in response to nitrogen form and cloning of NRT2.2[D]. Henan Agricultural University, 2014.
    [30]许金兰,谢小东,冯爽,等.烟草硝酸盐转运蛋白基因的克隆及表达分析[J].烟草科技, 2013(07):68-71+79.XU Jinlan, XIE Xiaodong, FENG Shuang, et al. Cloning and expression analysis of nitrate transporter gene(NtNRT2.3)from Nicotiana Tabacum[J]. Tobacco Science, 2013(07):68-71+79.
    [31]黄化刚,申燕,王卫峰,等.烟草硝酸盐转运蛋白基因Nt NRT2.4的克隆及表达分析[J].中国烟草学报, 2016, 22(1):84-91.HUANG Huagang, SHEN Yan, WANG Weifeng, et al. Cloning and characterization of NtNRT2.4 gene from Nicotiana tabacum L[J].Acta Tabacaria Sinica, 2016, 22(1):84-91.
    [32]Cove D J. The induction and repression of nitrate reductase in the fungus Aspergillus nidulans[J]. Biochimica et Biophysica Acta(BBA)-Enzymology and Biological Oxidation, 1966,113(1):51-56.
    [33]武丽.烤烟钼素营养作用机理及生产应用[D].安徽农业大学,2014.WU Li. Mechanism and application of molybdenum nutrition on flue-cured tobacco[D]. Anhui agricultural University, 2014.
    [34]Sood C R, Chanda S V, Singh Y D. Nitrate reductase activity of radish cotyledons as affected by phytohormones and different nitrogen sources[J]. Acta Physiologiae Plantarum, 2000, 22(4):477-482.
    [35]Klimenko S B, Peshkova A A, Dorofeev N V. Nitrate reductase activity during heat shock in winter wheat[J]. Journal of Stress Physiology&Biochemistry, 2006, 2(1):50-55.
    [36]Champigny M L. Integration of photosynthetic carbon and nitrogen metabolism in higher plants[J]. Photosynthesis Research, 1995,46(1-2):117-127.
    [37]杨晓玉.硝酸盐胁迫对黄瓜幼苗氮同化影响的研究[D].山东农业大学, 2008.YANG Xiaoyu. Study on effects of nitrate stress on nitrogen assimilation of cucumber(Cucummis sativus L.)seedlings[D].Shandong Agricultural University, 2008.
    [38]Cookson S J, Williams L E, Miller A J. Light-dark changes in cytosolicnitratepoolsdependonnitratereductaseactivityin Arabidopsis leaf cells[J]. Plant Physiology, 2005, 138(2):1097-1105.
    [39]Jonassen E M, Sévin D C, Lillo C. The bZIP transcription factors HY5 and HYH are positive regulators of the main nitrate reductase gene in Arabidopsis leaves, NIA2, but negative regulators of the nitrate uptake gene NRT1. 1[J]. Journal of plant physiology, 2009,166(18):2071-2076.
    [40]Taghavi T S, Babalar M. The effect of nitrate and plant size on nitrate uptake and in vitro nitrate reductase activity in strawberry(Fragaria×ananassa cv. Selva)[J]. Scientia horticulturae, 2007,112(4):393-398.
    [41]Balotf S, Kavoosi G, Kholdebarin B. Nitrate reductase, nitrite reductase,glutaminesynthetase,andglutamatesynthase expression and activity in response to different nitrogen sources in nitrogen‐starved wheat seedlings[J]. Biotechnology and applied biochemistry, 2016, 63(2):220-229.
    [42]李常健,林清华,张楚富.高等植物谷氨酰胺合成酶研究进展[J].生物学杂志, 2001(04):1-3.LI Changjian, LIN Qinghua, ZHANG Chufu. Progress of the studies on glutamine synthetase in higher plants[J]. Journal of Biology, 2001(04):1-3.
    [43]韩静静.硝酸盐胁迫对玉米幼苗氮同化影响的研究[D].内蒙古农业大学,2013HAN Jingjing. The effects of nitrate stress on nitrogen assimilation in maize(Zea mays L.)seedlings[D]. Inner Mongolia Agricultural University, 2013.
    [44]陈巍,罗金葵,尹晓明,等.硝酸盐在两个小白菜品种体内的分布及调配[J].中国农业科学, 2005(11):134-139.CHEN Wei, LUO Jinkui, YIN Xiaoming, et al. Distribution and remobilization of nitrate in two cultivars of Pakchoi plant[J].Scientia Agricultura Sinica, 2005(11):134-139.
    [45]Martinoia E(1992)Transport processes in vacuoles of higher plants.Botanica Acta. 105:232-245
    [46]De Angeli A, Monachello D, Ephritikhine G, et al. The nitrate/proton antiporter AtCLCa mediates nitrate accumulation in plant vacuoles[J]. Nature, 2006, 442(7105):939.
    [47]Geelen D, Lurin C, Bouchez D, et al. Disruption of putative anion channel gene AtCLC‐a in Arabidopsis suggests a role in the regulation of nitrate content[J]. The Plant Journal, 2000, 21(3):259-267.
    [48]Geelen D, Lurin C, Bouchez D, et al. Disruption of putative anion channel gene AtCLC‐a in Arabidopsis suggests a role in the regulation of nitrate content[J]. The Plant Journal, 2000, 21(3):259-267.
    [49]Brauer E K, Rochon A, Bi Y M, et al. Reappraisal of nitrogen use efficiency in rice overexpressing glutamine synthetase1[J].Physiologia plantarum, 2011, 141(4):361-372.
    [50]许自成,陈伟,黄平俊,等.影响烤烟叶片硝酸盐积累的因素分析[J].中国农学通报, 2004(06):47-49+95.XU Zicheng, CHEN Wei, HUANG Pingjun, et al. Factors of influencing nitrate accumulation in flue-cured tobacco leaf[J].Chinese Agricultural Science Bulletin, 2004(06):47-49+95.
    [51]赵晓丹.不同产区白肋烟质量特点及差异分析[D].河南农业大学, 2012.ZHAO Xiaodan. The study on quality characteres and differential analysis of burley[D]. Henan Agricultural University, 2012.
    [52]Li Y, Yang H, Chang D, et al. Biochemical, Physiological and Transcriptomic Comparison between Burley and Flue-Cured Tobacco Seedlings in Relation to Carbohydrates and Nitrate Content[J]. Molecules, 2017, 22(12):2126.
    [53]李亚飞,常栋,冯雨晴,杨惠娟,王景,王俊,史宏志.白肋烟烟叶氮同化能力及其对硝酸盐积累的影响[J/OL].中国烟草学报:1-14[2018-12-25]. https://doi.org/10.16472/j.chinatobacco.2018.165.LI Yafei, CHANG Dong, FENG Yuqing, et al. Nitrogen assimilation capacity of burley tobacco leaves and its effects on nitrate accumulation[J/OL]. Acta Tabacaria Sinica:1-14[2018-12-25].https://doi.org/10.16472/j.chinatobacco.2018.165.
    [54]Yanagisawa S. Transcription factors involved in controlling the expression of nitrate reductase genes in higher plants[J]. Plant Science, 2014, 229:167-171.
    [55]Yu L H, Wu J, Tang H, et al. Overexpression of Arabidopsis NLP7improves plant growth under both nitrogen-limiting and-sufficient conditions by enhancing nitrogen and carbon assimilation[J].Scientific reports, 2016, 6:27795.
    [56]Chaplin J F. Inheritance and Possible Use of Pale Yellow Character in Tobacco 1[J]. Crop Science, 1969, 9(2):169-172.
    [57]Henica, F. S. The inheritance of the white burley character in tobacco. Japanese Journal of Crop Science. 4, 281-282(1932).
    [58]许自成,张莉,石俊雄,等.施磷对烤烟硝酸盐和亚硝酸盐含量的影响[J].烟草科技, 2003(02):32-35.XU Zicheng, ZHANG Li, SHI Junxiong, et al. Effects of phosphate fertilizer on the content of nitrate and nitrite in flue-cured tobacco[J]. Tobacco Science, 2003(02):32-35.
    [59]刘扣珠,靳彤,王俊,等.自然贮藏条件下烤烟叶片和烟梗中TSNAs及其前体物含量的变化[J].烟草科技, 2018, 51(10):27-32+38.LIU Kouzhu, JIN Tong, WANG Jun, et al. TSNA contents and their precursors in lamina and midrib of flue-cured tobacco stored under natural conditions[J]. Tobacco Science, 2018, 51(10):27-32+38.
    [60]史宏志,韩锦峰.烤烟碳氮代谢几个问题的探讨[J].烟草科技,1998(02):34-36.SHI Hongzhi, HAN Jinfeng. Discussion on carbon and nitrogen metabolism of flue-cured tobacco[J]. Tobacco Science, 1998(02):34-36.
    [61]周晚来,刘文科,杨其长.光对蔬菜硝酸盐累积的影响及其机理[J].华北农学报, 2011, 26(S2):125-130.ZHOU Wanlai, LIU Wenke, YANG Qichang. Effect of light on nitrate accumulation in vegetables and the mechanism thereof[J].Acta Agriculturae Boreali-Sinica, 2011, 26(S2):125-130.
    [62]Borner T, Mendel R R, Schiemann J. Nitrate reductase is not accumulated in chloroplast-ribosome-deficient mutants of higher plants[J]. Planta, 1986, 169(2):202-207.
    [63]古战朝.烤烟主产区生态因子与烟叶品质的关系[D].河南农业大学, 2012.GU Zhanchao. Relationship between ecological factors and tobacco leaf’s quality in main tobacco-growing regions[D]. Henan Agricultural University, 2012.
    [64]陈永勤,冯勃,徐卫红,等.小白菜硝酸盐含量与光照度及氮代谢关键酶的相关性[J].食品科学, 2016, 37(13):183-188.CHEN Yongqin, FENG Bo, XU Weihong, et al. Relationship between nitrate contents of chinese cabbage(Brassica chinensis L.)and light intensity or key enzymes of nitrogen metabolism[J]. Food Science, 2016, 37(13):183-188.
    [65]Burton H R, Dye N K, Bush L P. Distribution of tobacco constituents in tobacco leaf tissue. 1. Tobacco-specific nitrosamines,nitrate, nitrite, and alkaloids[J]. Journal of Agricultural and Food Chemistry, 1992, 40(6):1050-1055.
    [66]倪晋山.小麦吸收、累积硝酸根的品种间差异[J].植物生理学报,1982(03):307-315.NI Jinshan. Varietal differences of nitrate uptake and accumulation by wheat(Triticum Aestivum)[J]. Journal of Plant Physiology and Molecular Biology, 1982(03):307-315.
    [67]许长蔼.植物体内NO_3~-可给性对硝酸还原酶活性的调节[J].植物生理学通讯, 1991(03):173-177.XU Changai. Regulation of nitrate reductase activity in vivo by nitrate availability in higher plants[J]. Plant Physiology Journal,1991(03):173-177.
    [68]高祖明,张耀栋,张道勇,等.氮磷钾对叶菜硝酸盐积累和硝酸还原酶、过氧化物酶活性的影响[J].园艺学报, 1989(04):293-298.GAO Zuming, ZHANG Yaodong, ZHANG Daoyong, et al. Effects of N, P, K treatments on the accumulation of nitrate and the activity of nitrate reductase and superoxidase in two leafy vegetables[J].Acta Horticulturae Sinica, 1989(04):293-298.
    [69]刘敏超.蔬菜的硝酸盐污染及防止措施[J].现代化农业,2002(04):6-7.LIU Minchao. Nitrate pollution of vegetables and preventive measures[J]. Modernizing Agriculture, 2002(04):6-7.
    [70]Effects of solution pH, temperature,nitrate/ammonium ratios and inhibitors on ammonium and nitrate uptake by arctic coffee in short-term solution culture. Philippe V, J. Z Robert, S.B.Caroline.Journal of Plant Nutrition. 1998
    [71]喻奇伟.水钾耦合对烤烟钾含量及碳氮代谢的影响[D].河南农业大学, 2008.YU Qiwei. Effect of water and potassium(K)fertilizer coupling on the potassium content and carbon and nitrogen metabolism of fluecured tobacco[D]. Henan Agricultural University, 2008.
    [72]陈贵军,张水成,许自成.土壤肥力对烤烟叶片硝酸盐积累的影响[J].中国农学通报, 2005(08):200-203.CHEN Guijun, ZHANG Shuicheng, XU Zicheng. Effects of soiI fertility on Nitrate accumulation in flue-cured tobacco leaf[J].Chinese Agricultural Science Bulletin, 2005(08):200-203.
    [73]陈伟,刘桂珍,杨树林,肖汉乾,许自成.烤烟叶片硝酸盐、亚硝酸盐含量与土壤养分的关系[J].河南农业科学, 2004(06):39-42.CHEN Wei, LIU Guizhen, YANG Shulin, et al. Relationship betweensoilnutrientsandnitrateandnitritecontentin flue-curedtobaccoleaf[J].JournalofHenan Agricultural Sciences,2004(06):39-42.
    [74]李天贵,周兆德,黄启为.氮肥种类对蔬菜产量和品质影响的研究[J].湖南农学院学报,19911):17-22.LI Tiangui, ZHOU Zhaode, HUANG Qiwei. Effect of N-fertilizer source on yield and quality of vegetables[J]. Gournal of Hunan agricultural colleage, 1991(01):17-22.
    [75]朱天琦,刘晓静,张晓玲.氮营养调控对紫花苜蓿根系形态及其解剖结构的影响[J].草地学报, 2016, 24(6):1290-1295.ZHU Tianqi, LIU Xiaojing, ZHANG Xiaoling. Effects of nitrogen on root morphology and anatomical structure of Alfalfa[J]. Acta Agrestia Sinica, 2016, 24(6):1290-1295.
    [76]孙榅淑,杨军杰,周骏,等.不同氮素形态对烟草硝态氮含量和TSNA形成的影响[J].中国烟草学报, 2015, 21(04):78-84.SUN Wenshu, YANG Junjie, ZHOU Jun, et al. Effect of different nitrogen forms on nitrate nitrogen content and TSNAs formation in tobacco[J]. Acta Tabacaria Sinica, 2015, 21(04):78-84.
    [77]林春华,黄亮华,陈永泉,等.缺氮、磷、钾、钙、镁对芥蓝硝酸盐积累、硝酸还原酶和过氧化物酶活性的影响[J].华南农业大学学报, 1998(04):58-61.LIN Chunhua, HUANG Lianghua, CHEN Yongquan, et al. Effect of N,P,K,Ca and Mg deficiency on nitrate accumulation, nitrate reductase and peroxidase activity in Chinese Kale[J]. Journal of South China Agricultural University, 1998(04):58-61.
    [78]何天秀,何成辉,吴德意.蔬菜中硝酸盐含量及其与钾含量的关系[J].农业环境科学学报, 1992(05):209-211+242.HE Tianxiu, HE Chenghui, WU Deyi. Nitrate content in vegetables and its relationship with potassium content[J]. Journal of AgroEnvironment Science, 1992(05):209-211+242.
    [79]杨少海,徐培智,刘国坚.降低蔬菜硝酸盐含量的农业措施[J].土壤与环境, 1999(03):235-237.YANG Shaohai, XU Peizhi, LIU Guojian. Agricultural measures to decrease nitrate in vegetables[J]. Soil and Environmental sciences,1999(03):235-237.
    [80]Obata H, Umebayashi M. Effect of zinc deficiency on protein synthesis in cultured tobacco plant cells[J]. Soil Science and Plant Nutrition, 1988, 34(3):351-357.
    [81]Cieslik E, Sikora E. Correlation between the levels of nitrates and nitrites and the contents of potassium, calcium and magnesium in potato tubers[J]. Food Chemistry, 1998, 63(4):525-528.
    [82]Deng M, Moureaux T, Caboche M. Tungstate, a molybdate analog inactivating nitrate reductase, deregulates the expression of the nitrate reductase structural gene[J]. Plant physiology, 1989, 91(1):304-309.
    [83]赵静,白清云,帕尼古丽,等.钼对降低蔬菜硝酸盐积累的效应研究[J].农业环境保护, 2001(04):238-239.ZHAO Jing, BAI Qingyun, PANI Guli, et al. Alleviation of nitrate accumulation in vegetables by application of molybdenum[J].Agro-Environment Protection,2001(04):238-239.
    [84]毕理智,张锐,王海景,张国进,杜文波.不同作物滴灌施肥效果分析[J].山西农业科学, 2008(10):50-52.BI Lizhi, ZHANG Rui, WANG Haijing, et al. Comparison of Drip Fertigation on Different Crop System[J]. Journal of Shanxi Agricultural Sciences, 2008(10):50-52.
    [85]张学军,赵营,陈晓群,吴礼树,胡承孝.滴灌施肥中施氮量对两年蔬菜产量、氮素平衡及土壤硝态氮累积的影响[J].中国农业科学, 2007(11):2535-2545.ZHANG Xuejun, ZHAO Ying, CHEN Xiaoqun, et al. Effects of Application of Nitrogen on Vegetable Yield, Nitrogen Balance and Soil Nitrogen Accumulation Under Two Years'Drip Fertigation[J].Scientia Agricultura Sinica, 2007(11):2535-2545.
    [86]石俊雄,郑少清,刁朝强,等.有机肥及施氮水平对烟叶质量和可用性的影响[J].中国烟草科学, 2004, 25(2):42-45.SHI Junxiong, ZHENG Shaoqing, DIAO Chaoqiang, et al. Effects of manure application on quality and usability of leaf tobacco under different levels of nitrogen[J]. Chinese Tobacco Science, 2004,25(2):42-45.
    [87]李元实,王莹,赵铭钦,孙国伟,韩富根.种植密度及留叶数对延边生态区烤烟主要含氮化合物含量的影响[J].安徽农业科学,2008(22):9580-9581+9584.LI Yuanshi, WANG Ying, ZHAO Mingqin, et al. Effects of Plant Population and Remained Leaves Number on Contents of Main N Containing Compounds in Flue-cured Tobacco[J]. Journal of Anhui Agricultural Sciences, 2008(22):9580-9581+9584.
    [88]张长华,王智明,陈叶君,潘文杰,黄建国.连作对烤烟生长及土壤氮磷钾养分的影响[J].贵州农业科学, 2007(04):62-65.ZHANG Changhua, WANG Zhiming, CHEN Yejun, et al. The Effect of Continuous Cropping on Tobacco Growth and Soil Nutrients[J]. Guizhou Agricultural sciences, 2007(04):62-65.
    [89]刘方,何腾兵,刘元生,罗海波,舒英格.长期连作黄壤烟地养分变化及其施肥效应分析[J].烟草科技, 2002(06):30-33.LIU Fang, HE Tengbing, LIU Yuansheng, et al. Nutrient changes of yellow soil successively planted flue-cured tobacco and its fertilization effect[J]. Tobacco Science, 2002(06):30-33.
    [90]解莹莹.施肥和打顶对烤烟中主要含氮化合物含量的影响[D].河南农业大学, 2004.XIE Yingying. Effects of fertilization and topping on the content of important nitric compounds in flue-cured tobacco[D]. Henan Agricultural University,2004.
    [91]蔡长春,冯吉,程玲,等.白肋烟烟碱转化性状的主基因+多基因遗传分析[J].中国农学通报, 2013, 29(34):61-65.CAI Changchun, FENG Ji, CHENG Ling, et al. Genetic analysis of nicotine conversion of burley tobacco using mixed model of major gene and polyge[J]. Chinese Agricultural Science Bulletin, 2013,29(34):61-65.
    [92]Lu J, Zhang L, Lewis R S, et al. Expression of a constitutively activenitratereductasevariantintobaccoreducestobacco‐specific nitrosamine accumulation in cured leaves and cigarette smoke[J]. Plant biotechnology journal, 2016, 14(7):1500-1510.
    [93]QuilleréI, DufosséC, Roux Y, et al. The effects of deregulation of NR gene expression on growth and nitrogen metabolism of Nicotiana plumbaginifolia plants[J]. Journal of Experimental Botany, 1994, 45(9):1205-1211.
    [94]Oliveira I C, Brears T, Knight T J, et al. Overexpression of cytosolic glutamine synthetase. Relation to nitrogen, light, and photorespiration[J]. Plant Physiology, 2002, 129(3):1170-1180.
    [95]Zheng J S, Yu C M, Chen P, et al. Characterization of a glutamine synthetase gene BnGS1-2 from ramie(Boehmeria nivea L. Gaud)and biochemical assays of BnGS1-2-over-expressing transgenic tobacco[J]. Acta physiologiae plantarum, 2015, 37(1):1742.
    [96]冯慧敏,陆宏,王汉卿,等.水稻硝酸盐转运蛋白基因OsNPF7.9在氮素积累和转运中的功能研究[J].中国水稻科学,2017, 31(5):457-464.FENG Huimin, LU Hong, WANG Hanqing, et al. Function analyses of rice nitrate transporter gene OsNPF7.9 in nitrogen accumulation and transport[J]. Chinese Journal of Rice Science, 2017, 31(5):457-464.
    [97]陈景光,张勇,谭雅文,等.过量表达Os NRT2.1对水稻日本晴生长和氮素利用效率的影响[J].分子植物育种, 2016, 14(1):1-9.FENG Yuqing, YANG Huijuan, SHI Hongzhi, et al. The effects of OsNRT2.1 over-expression on plant growth and nitrogen use efficiency in rice Nipponbare(Oryza sativa L. ssp. japonica)[J].Molecular Plant Breeding, 2016, 14(1):1-9.
    [98]朱思慧.吊兰硝酸盐转运蛋白编码基因的克隆及功能分析[D].沈阳农业大学, 2018.ZHU Sihui. The cloning and functional analysis of NRT genes from Chlorophytum comosum[D]. Shenyang Agricultural University,2018.
    [99]Pengelly J J L, F?rster B, von Caemmerer S, et al. Transplastomic integration of a cyanobacterial bicarbonate transporter into tobacco chloroplasts[J]. Journal of experimental botany, 2014, 65(12):3071-3080.
    [100]Lin M T, Occhialini A, Andralojc P J, et al.β‐Carboxysomal proteins assemble into highly organized structures in Nicotiana chloroplasts[J]. The Plant Journal, 2014, 79(1):1-12.
    [101]Uematsu K, Suzuki N, Iwamae T, et al. Increased fructose1, 6-bisphosphate aldolase in plastids enhances growth and photosynthesis of tobacco plants[J]. Journal of Experimental Botany, 2012, 63(8):3001-3009.
    [102]沈善敏.长期土壤肥力试验的科学价值.植物营养与肥料学报,1995,1(1), 1-9.SHEN Shanmin. The scientific value of long-term soil fertility experiment. Journal of Plant Nutrition and Fertilizers, 1995, 1(1), 1-9.
    [103]Xu GH, Fan XR, Miller AJ. Plant nitrogen assimilation and use efficiency. Annu Rev Plant Biol, 2012, 63(63):153–182
    [104]梁景霞.烟草种质ISSR标记及对氮素响应的生理遗传与差异蛋白质组学研究. 2008,(Doctoral dissertation,福建农林大学).LIANGJingxia.SSRanalysisoftobaccogermplasmand physiological genetics and differential proteomics in response to nitrogen nutrition Fujian 2008,(Doctoral dissertation, Agricultural University).
    [105]Kling J G,Heuberger H T, Oikeh S O, et al. Potential for developing nitrogen-use efficientmaize for low input agricultural systems in the moist savanna of Africa. CIMMYT, Mexico, 1997, 490-501.
    [106]李红利.复硝酚钠及其组分对韭菜硝酸盐累积污染的减控效应研究[D].河北农业大学,2015.LI Hongli. Effects of compound sodium nitrophenolate and its components on the control of nitrate accumulation in the leaves of Chinese chive[D]. Agricu;ltural University of Hebei,2015.
    [107]Han Y, Liao Q, Yu Y, et al. Nitrate reutilization mechanisms in the tonoplast of two Brassica napus genotypes with different nitrogen use efficiency[J]. Acta physiologiae plantarum, 2015, 37(2):42.
    [108]田发祥.湖南双季稻专用稳定性复混肥料的氮抑制剂及其配比研究[D].中南大学,2013.TIAN Faxiang. Study on N-Inhibitor and its ratio of special stable compound for double-rice in Hunan province[D]. Central South University, 2013.
    [109]苗玉新.降低蔬菜中硝酸盐含量的途径[J].农业系统科学与综合研究,1998(01):69-71.MIAO Yuxin. Ways to reduce nitrate content in vegetables[J].System Sciences and Comprehensive Studies in Agriculture,1998(01):69-71.
    [110]傅柳松,刘超,吴方正.钼和双氰胺降低蔬菜硝酸盐积累的效应研究[J].环境污染与防治, 1994(03):4-6+45.FU Liusong, LIU Chao, WU Fangzheng. The pollution of field atmosphere and non-target crop caused by Saikuzuo used in rice field[J]. Environmental Pollution&Control,1994(03):4-6+45.
    [111]Li Y, Shi H, Yang H, et al. Difference between Burley Tobacco and Flue-Cured Tobacco in Nitrate Accumulation and Chemical Regulation of Nitrate and TSNA Contents[J]. Journal of Chemistry,2017, 2017.
    [112]Li Y, Chang D, Yang H, et al. Metabolome and molecular basis for carbohydrate increase and nitrate reduction in burley tobacco seedlings by glycerol through upregulating carbon and nitrogen metabolism[J]. Scientific reports, 2018, 8(1):13300.
    [113]Lin Y, Zhang J, Gao W, et al. Exogenous trehalose improves growth under limiting nitrogen through upregulation of nitrogen metabolism[J]. BMC plant biology, 2017, 17(1):247.
    [114]郭卫华,赵小明,杜昱光.壳寡糖对烟草幼苗生长和光合作用及与其相关生理指标的影响[J].植物生理学通讯, 2008, 44(6):1155-1157.GUO Weihua,ZHAOXiaoming,DU Yuguang.Effectsof oligochitosanon the growth and photosynthesis and physiological index related to photosynthesis of tobacco seedlings[J]. Plant Physiology Journal, 2008, 44(6):1155-1157.
    [115]王德宝,郜海民,史宏志,等.氮肥用量和基追肥比例对白肋烟干物质积累和氮肥利用率的影响[J].河南农业大学学报, 2011,45(2):155-159.WANG Debao, HAO Haimin, SHI Hongzhi, et al. Effects of the dosage of nitrogen and the ratio between base fertilizer and dressing on dry matter accumulation and fertilizer nitrogen recovery of burley tobacco[J]. Journal of Henan Agricultural University, 2011,45(2):155-159.
    [116]孙泽东,梁晓芳,石屹,马兴华.滴灌施氮对烤烟氮素吸收利用及土壤无机氮分布的影响[J].中国土壤与肥料, 2018(05):46-51.SUN Zedong, LIANG Xiaofang, SHI Yi, et al. Effect of drip nitrogen fertigation on flue-cured tobacco nitrogen absorption,utilization and distribution of soil miner-al nitrogen[J]. Soils and Fertilizers Sciences in China,2018(05):46-51.
    [117]高秀瑞,陈贵林.甘氨酸部分替代硝态氮对不结球白菜和生菜生长及硝酸盐积累的影响[J].河北农业大学学报, 2003(01):40-43.GAO Xiurui, CHEN Guilin. Effect of replacement of nitrate by Gly on growth and accumulation of nitrate of nonheading Chinese cabbage and lettuce[J].Journal of Hebei Agricultural University,2003(01):40-43.
    [118]席奇亮,杨铁钊,周方,等.水肥一体化条件下烤烟氮素营养高效利用研究[J].中国烟草学报, 2018, 24(02):74-83.XI Qiliang, YANG Tiezhao, ZHOU Fang, et al. Study on effective utilization of nitrogen nutrition in flue-cured tobacco production under integration of water and fertilizer[J]. Acta Tabacaria Sinica,2018, 24(02):74-83.
    [119]张莉.施肥对烤烟硝酸盐和亚硝酸盐含量的影响[D].河南农业大学, 2004.ZHANG Li. Effects of fertilization on the nitrate and nitrite contents of flue-cured tobacco[D]. Henan Agricultural University,2004.
    [120]吕大树.烟田施用硼锌钼微肥效果研究[D].湖南农业大学, 2013.LV Dashu. Study on the effect of boron, zinc and molybdenum nutrition in flue-cured tobacco fields[D]. Hunan Agricultural University, 2013.
    [121]陈永安,武丽,李余湘,等.钼营养对烤烟光合色素、光合特性和水分利用率的影响[J].安徽农业大学学报, 2012, 39(3):421-425.CHEN Yongan, WU Li, LI Yuxiang, et al. Effects of molybdenum on photosynthetic pigment characteristics and water efficiency of flue-cured tobacco[J]. Journal of Anhui Agricultural University,2012, 39(3):421-425.
    [122]Steingr?ver E, Ratering P, Siesling J. Daily changes in uptake,reduction and storage of nitrate in spinach grown at low light intensity[J]. Physiologia plantarum, 1986, 66(3):550-556.
    [123]冯俊喜,王树声,石屹,张继光,郑林林,李湘伟,胡庆辉,王浩浩.山东烟区不同种植模式土壤微生物群落特征研究[J].中国烟草科学, 2011, 32(02):38-42.FENG Junxi, WANG Shusheng, SHI Yi, et al. Characteristics of Soil Microbial Community under Different Cropping Systems in Flue-cured Tobacco Growing Areas of Shandong Province[J].Chinese Tobacco Science, 2011, 32(02):38-42.
    [124]魏全全,芶久兰,赵欢,张萌,周开芳,左明玉,苟世新,邵代兴,肖厚军.黄壤区烤烟轮作与连作根系形态、产量及养分吸收的变化[J].西南农业学报, 2018, 31(11):2294-2299.WEI Quanquan, GOUNJiulan, ZHAO Huan, et al. Changes of root morphology yield and nutrients uptake of flue-cured tobacco rotation and continuous cropping in yellow soil[J]. Southwest China Journal of Agricultural Sciences, 2018, 31(11):2294-2299.
    [125]许传强,李天来,齐红岩.嫁接对网纹甜瓜光合特性、生长状况及产量的影响[J].中国西瓜甜瓜, 2005,(2):1-3. DOI:10.3969/j.issn.1673-2871.2005.02.001.XU Chuanqiang, LI Tianlai, QI Hongyan. Effect of grafting on photosynthetic characteristics and yield of muskmelon[J].China watermelon and muskmelon, 2005,(2):1-3. DOI:10.3969/j.issn.1673-2871.2005.02.001.
    [126]齐红岩,李天来,刘轶飞,等.嫁接对薄皮甜瓜光合特性、产量与含糖量的影响[J].沈阳农业大学学报, 2006,(2):155-158.DOI:10.3969/j.issn.1000-1700.2006.02.007.QI Hongyan, LI Tianlai, LIU YIfei. Effects of grafting on photosynthesis characteristics, yield and sugar content in melon[J].Journal of Shenyang Agricultural University, 2006,(2):155-158.DOI:10.3969/j.issn.1000-1700.2006.02.007.
    [127]付玲,白小梅,杨显贺,等.嫁接辣椒光合特性及其对产量和品质的影响[J].园艺学报,2013,(3):449-457.FU Ling, BAI Xiaomei, YANG Xianhe, et al. Effects of grafted on photosynthetic characteristics yield and quality of pepper[J]. Acta Horticulturae Sinica, 2013,(3):449-457.
    [128] Agbaria H, Heuer B, Zieslin N. Shoot-root interaction effects on nitrate reductase and glutamine synthetase activities in rose(Rosa×hybrida cvs. Ilseta and Mercedes)graftlings[J]. Journal of Plant Physiology, 1996, 149(5):559-563.
    [129]王志冉.嫁接对甜瓜幼苗氮代谢的影响[D].沈阳农业大学, 2018.WANG Zhiran. Effects of grafting on nitrogen metabolism in melon seedlings[D]. Shenyang Agricultural University, 2018.
    [130]梅芳,杨铁钊,刘剑君,等.嫁接对烤烟氮代谢关键酶活性及叶绿素含量的影响[J].西北农业学报, 2012, 21(05):132-135+150.MEI Fang,YANG Tiezhao, LIU Jianjun, et al. Effects of grafting on tobacco key enzyme activities of nitrogen metabolism and the contents of chlorophyll[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2012, 21(05):132-135+150.
    [131]Gaudinier A, Rodriguez-Medina J, Zhang L, et al. Transcriptional regulation of nitrogen-associated metabolism and growth[J].Nature, 2018:1.

© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号

地址:北京市海淀区学院路29号 邮编:100083

电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700