山西朔州甘草中重金属及有机氯农药残留含量测定
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摘要
目的建立甘草药材中重金属及有机氯残留含量测定的方法,以期为山西朔州甘草的质量评价提供依据。方法通过电感耦合等离子体质谱法(ICP-MS)测定甘草中Cu、As、Cd、Hg、Pb 5种重金属的含量;气相色谱法测定甘草中α-BHC、β-BHC、γ-BHC、δ-BHC、p,p″-DDE、p,p″-DDD、p,p″-DDT、o,p″-DDT、五氯硝基苯(PCNB) 9种有机氯残留的含量。结果实验所用甘草中Cu、As、Cd、Hg、Pb 5种重金属含量范围依次为9. 810-10. 680 mg/kg,0. 195-0. 224 mg/kg,0. 026-0. 029 mg/kg,0. 025-0. 034 mg/kg,0. 281-0. 336 mg/kg,9种有机氯农药残留的含量中总六六六的含量范围为5. 564-17. 278μg/kg,总滴滴涕含量范围为0-3. 599μg/kg,五氯硝基苯(PCNB)的含量范围为7. 249-17. 192μg/kg。结论本实验采用ICP-MS实现了对甘草中5种重金属元素含量的同时准确测定;采用气相色谱法实现了对甘草中有机氯农药残留量的检测。该两种方法操作简便、灵敏度高、准确性好,所测甘草中5种重金属和9种有机氯农药残留量均符合《中国药典》(2015版)的限量要求。
Objective To establish a method for determining the contents of heavy metals and organochlorine residues in licorice to provide a basis for the quality evaluation of licorice in Shuozhou city of Shanxi Province. Methods The contents of 5 heavy metals such as Cu,As,Cd,Hg and Pb in licorice were determined by inductively coupled plasma mass spectrometry( ICP-MS). And the contents of 9 organochlorine pesticides residues such as α-BHC,β-BHC,γ-BHC,δ-BHC,p,p″-DDE,p,p″-DDD,p,p″-DDT,o,p″-DDT and PCNB in licorice were determined by gas chromatography( GC). Results The contents of Cu,As,Cd,Hg and Pb were9. 810-10. 680 mg/kg,0. 195-0. 224 mg/kg,0. 026-0. 029 mg/kg,0. 025-0. 034 mg/kg and 0. 281-0. 336 mg/kg,respectively,and the contents of the total BHC,the total DDT and PCNB were 5. 564-17. 278 μg/kg,0-3. 599 μg/kg and 7. 249-17. 192μg/kg,respectively. Conclusion ICP-MS may accurately determine the contents of five heavy metal elements in licorice. The gas chromatography may detect the organochlorine pesticide residues in licorice. The two methods are simple,sensitive and accurate. The contents of heavy metals and the organochlorine pesticides residue are in accord with China Pharmacopoeia( 2015 Edition) in all these regions.
Objective To establish a method for determining the contents of heavy metals and organochlorine residues in licorice to provide a basis for the quality evaluation of licorice in Shuozhou city of Shanxi Province. Methods The contents of 5 heavy metals such as Cu,As,Cd,Hg and Pb in licorice were determined by inductively coupled plasma mass spectrometry( ICP-MS). And the contents of 9 organochlorine pesticides residues such as α-BHC,β-BHC,γ-BHC,δ-BHC,p,p″-DDE,p,p″-DDD,p,p″-DDT,o,p″-DDT and PCNB in licorice were determined by gas chromatography( GC). Results The contents of Cu,As,Cd,Hg and Pb were9. 810-10. 680 mg/kg,0. 195-0. 224 mg/kg,0. 026-0. 029 mg/kg,0. 025-0. 034 mg/kg and 0. 281-0. 336 mg/kg,respectively,and the contents of the total BHC,the total DDT and PCNB were 5. 564-17. 278 μg/kg,0-3. 599 μg/kg and 7. 249-17. 192μg/kg,respectively. Conclusion ICP-MS may accurately determine the contents of five heavy metal elements in licorice. The gas chromatography may detect the organochlorine pesticide residues in licorice. The two methods are simple,sensitive and accurate. The contents of heavy metals and the organochlorine pesticides residue are in accord with China Pharmacopoeia( 2015 Edition) in all these regions.
引文
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[10]林丽,晋玲,高素芳,等.微波消解-电感耦合等离子体质谱仪测定藏药黑果枸杞中5种元素含量[J].药物分析杂志,2018,38(12):2135-2140.
[11]李耀磊,张志成,金红宇,等. ICP-MS法测定复方苦参注射液中铜、砷、镉、汞、铅元素的残留[J].药物分析杂志,2018,38(10):1781-1787.
[12]赵蓉.我国8种中药材重金属污染的系统评价[D].北京:北京中医药大学,2016:1-56.
[13]朱振兴.中药甘草饮片中的铅对健康的影响[J].中医学报,2016,31(220):1357-1359.
[14]朱美霖,朱新忠,冯宁川.重金属在甘草中的健康风险评估及限量浓度测定[J].中成药,2016,38(8):1771-1775.
[2]张明发,沈雅琴.甘草及其活性成分抗炎与抗炎机制的研究进展[J].现代药物与临床,2011,26(4):261-268.
[3] Aghamohammadi AA,Zargar A,Oveisi S,et al. To evaluate of the effect of adding licorice to the standard treatment reg Inen of Helicobacter pylori[J]. Braz J Infect Dis,2016,20(6):534-538.
[4]国家药典委员会.《中华人民共和国药典》(四部)[M].北京:中国医药科技出版社,2015:6.
[5]黄明进,王文全,魏胜利.我国甘草药用植物资源调查及质量评价研究[J].中国中药杂志,2010,35(8):947-952.
[6]魏胜利,王文全,王继永,等.我国不同产区野生与栽培甘草的甘草酸含量及其影响因子的初步研究[J].中国中药杂志,2012,37(10):1341-1345.
[7]彭启志.中药材种植基地土壤及中药材中重金属赋存水平的环境变化分析[J].中国医药科学,2015,5(4):210-213.
[8]左忠,牛艳,张安东,等.人工栽培甘草五种重金属含量研究[J].黑龙江农业科学,2018,41(12):90-93.
[9]高振杰,高红霞,刘英莉,等. ICP-MS法测定5种中药材粉剂中5种重金属的含量[J].中国药房,2015,26(27):3847-3849.
[10]林丽,晋玲,高素芳,等.微波消解-电感耦合等离子体质谱仪测定藏药黑果枸杞中5种元素含量[J].药物分析杂志,2018,38(12):2135-2140.
[11]李耀磊,张志成,金红宇,等. ICP-MS法测定复方苦参注射液中铜、砷、镉、汞、铅元素的残留[J].药物分析杂志,2018,38(10):1781-1787.
[12]赵蓉.我国8种中药材重金属污染的系统评价[D].北京:北京中医药大学,2016:1-56.
[13]朱振兴.中药甘草饮片中的铅对健康的影响[J].中医学报,2016,31(220):1357-1359.
[14]朱美霖,朱新忠,冯宁川.重金属在甘草中的健康风险评估及限量浓度测定[J].中成药,2016,38(8):1771-1775.