生物素化微细纤维肽修饰IGF-1对急性心肌梗死兔左心室重构的影响
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摘要
目的探讨生物素化微细纤维肽修饰胰岛素样生长因子-1(IGF-1)对急性心肌梗死兔左心室重构的影响。方法将28只健康的、雄性新西兰白兔随机分为对照组和实验1组、实验2组、实验3组,每组7只。将实验1、2、3组的冠状动脉左前降支结扎来建立兔急性心肌梗死模型,对照组不结扎冠状动脉左前降支。模型建立后,实验1组注射生物素化微细纤维肽修饰的IGF-1,实验2组注射未修饰的IGF-1,实验3组和正常对照组注射0.9%氯化钠注射液。分别检查各组干预6～8h后和干预1、8周后的心电图(ECG),干预1、4、8周后左心室舒张末期内径(LVEDd)、左心室射血分数(LVEF)、心输出量(CO)的水平。结果实验3组干预8周后ECG示出现宽大的病理性Q波。实验1、2、3组干预1、4、8周后LVEDd水平均显著高于对照组,LVEF、CO水平均显著低于对照组(均P<0.05)。干预1、4、8周后,实验1、2组LVEDd水平均显著低于实验3组,且实验1组又均显著低于实验2组(均P<0.05);实验1、2组LVEF、CO水平均显著高于实验3组,且实验1组又均显著高于实验2组(均P<0.05)。结论生物素化微细纤维肽修饰IGF-1能显著改善兔急性心肌梗死后心脏的缺血及左心室重构,且有利于心肌功能的修复。
Objective To investigate the effect of biotinylated microfiber peptides-modified insulin-like growth factor-1(IGF-1)on the left ventricular remodeling in rabbits with acute myocardial infarction(AMI).Methods Twenty-eight male New Zealand white rabbits were randomly divided into control group,experimental group 1,experimental group 2 and experimental group 3,with 7 rabbits in each group.The AMI was induced by ligating the left anterior descending coronary artery in experimental groups.The control group received sham operation only.After model establishment,the experimental group 1 was injected with biotinylated microfiber peptides-modified IGF-I,and the experimental group 2 with unmodified IGF-I.Both control group and experimental group 3 were given 0.9%sodium chloride injection.The electrocardiogram(ECG)was observed after treatment for 6-8 hours and intervention for 1 and 8 weeks.Furthermore,left ventricular end-diastolic diameter(LVEDd),left ventricular ejection fraction(LVEF)and cardiac output(CO)were measured after 1,4,and 8 weeks of intervention.Results In experimental group 3,ECG still showed large pathological Q waves after 8 weeks of intervention.Compared with control group,the LVEDd increased and LVEF and CO decreased in all the three experimental groups after 1,4 and 8 weeks of intervention(P<0.05).Compared with group 3,the LVEDd reduced and the LVEF and CO increased in both group 1 and group 2(P<0.05).Compared with group 2,the LVEDd decreased and the LVEF and CO increased in group 1(P<0.05).Conclusion Biotinylated microfiber peptides-modified IGF-1 can significantly improve cardiac ischemia and left ventricular remodeling and is beneficial to the repair of myocardial function in rabbits with AMI.
Objective To investigate the effect of biotinylated microfiber peptides-modified insulin-like growth factor-1(IGF-1)on the left ventricular remodeling in rabbits with acute myocardial infarction(AMI).Methods Twenty-eight male New Zealand white rabbits were randomly divided into control group,experimental group 1,experimental group 2 and experimental group 3,with 7 rabbits in each group.The AMI was induced by ligating the left anterior descending coronary artery in experimental groups.The control group received sham operation only.After model establishment,the experimental group 1 was injected with biotinylated microfiber peptides-modified IGF-I,and the experimental group 2 with unmodified IGF-I.Both control group and experimental group 3 were given 0.9%sodium chloride injection.The electrocardiogram(ECG)was observed after treatment for 6-8 hours and intervention for 1 and 8 weeks.Furthermore,left ventricular end-diastolic diameter(LVEDd),left ventricular ejection fraction(LVEF)and cardiac output(CO)were measured after 1,4,and 8 weeks of intervention.Results In experimental group 3,ECG still showed large pathological Q waves after 8 weeks of intervention.Compared with control group,the LVEDd increased and LVEF and CO decreased in all the three experimental groups after 1,4 and 8 weeks of intervention(P<0.05).Compared with group 3,the LVEDd reduced and the LVEF and CO increased in both group 1 and group 2(P<0.05).Compared with group 2,the LVEDd decreased and the LVEF and CO increased in group 1(P<0.05).Conclusion Biotinylated microfiber peptides-modified IGF-1 can significantly improve cardiac ischemia and left ventricular remodeling and is beneficial to the repair of myocardial function in rabbits with AMI.
引文
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[11]王雪芳,刘艳明.氧化苦参碱对急性心肌梗死家兔心功能和左心室重构的影响[J]中国应用生理学杂志,2015,31(2):123-126.
[2]黎叶飞,盛臻强,王毅,等.内皮祖细胞和胰岛素样生长因子-1联合移植治疗急性心肌梗死的实验研究[J].南通大学学报(医学版),2016,36(1):29-32,87.
[3]孙阿林,张光芳,肖丽,等.冻干重组人脑利钠肽对兔心肌梗死后心肌组织病理改变的影响及机制[J].山东医药,2016,56(20):31-33,111.
[4]龙湘党,赵康,叶剑荣,等.兔急性心肌梗死模型的建立与心功能、病理演变的动态研究[J].中国医学影像学杂志,2014,9(9):655-658.
[5]孙月,杨道玲,王禹雪,等.超声心动图常规及新技术评价兔心肌梗死模型[J].中国超声医学杂志,2016,32(7):648-651.
[6] Han D S,Hsiao M Y,Wang T G,et al.Relation between serum myokines and phaseⅡcardiac rehabilitation[J].Medicine(Baltimore),2017,96(16):e6579.
[7] Gómez Mauricio G,Moscoso I,Martín Cancho M F,et al.Combined administration of mesenchymal stem cells overexpressing IGF-1and HGF enhances neovascularization but moderately improves cardiac regeneration in a porcine model[J].Stem Cell Res Ther,2016,7(1):94.
[8] Song C L,Liu B,Diao H Y,et al.Down-regulation of microRNA-320suppresses cardiomyocyte apoptosis and protects against myocardial ischemia and reperfusion injury by targeting IGF-1[J].Oncotarget,2016,7(26):39740-39757.
[9] Mao C,Hou X,Wang B,et al.Intramuscular injection of human umbilical cord-derived mesenchymal stem cells improves cardiac function in dilated cardiomyopathy rats[J].Stem Cell Res Ther,2017,8(1):18.
[10]胡斯楞,魏芳晶.G-CSF和IGF-1抑制病理性心室重构及改善心功能的作用机制[J].医学综述,2015,21(23):4269-4271.
[11]王雪芳,刘艳明.氧化苦参碱对急性心肌梗死家兔心功能和左心室重构的影响[J]中国应用生理学杂志,2015,31(2):123-126.