环丙沙星和铜复合污染对小麦早期生长的影响
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摘要
以小麦为供试生物,采用高等植物毒理诊断试验方法研究了土壤外源环丙沙星和铜复合污染对小麦早期生长的影响,以探讨粪源污染土壤潜在的生态风险。结果表明:在供试浓度范围内,铜和环丙沙星单一污染下,小麦种子早期生长指标均产生了不同程度的响应,其中芽长和根长主要表现为抑制效应,而小麦种子发芽率未受影响;指标对污染物反应敏感顺序为芽长>根长>发芽率、环丙沙星与铜复合污染对小麦根长和芽长的影响表现出不同的效应;20和80 mg·kg-1铜与环丙沙星复合时相对单一作用对小麦根长的抑制有毒性增强的趋势,表现为协同效应;而200 mg·kg-1单一铜处理对小麦芽长的抑制效应在与环丙沙星复合后有所缓解,表现为拮抗特征;环丙沙星和铜在环境介质中交互作用产生络合物可能是导致不同生物效应的原因。
The effect of soil exogenous ciprofloxacin and copper combined pollution on the early growth of wheat was studied by the toxicological diagnosis method of higher plant. The potential ecological risks of soil contamination derived from livestock manure were investigated. Results indicated the early growth indexes of wheat expressed different degrees of responses to single copper or ciprofloxacin exposure. The wheat shoot and root length were mainly inhibited,while the seed germination rate was unaffected. The sensitivities of the three indexes to the two pollutants were in order of shoot length > root length > germination rate. Ciprofloxacin and copper combined pollution showed different effects on the wheat roots and shoot length. Compared with single copper exposure,inhibition effect on wheat root length was enhanced by the combined treatment of 20mg·kg-1copper and 80 mg·kg-1ciprofloxacin,showing a synergistic effect,while the inhibition effect on wheat shoot length by the addition of 200 mg·kg-1of copper eased after ciprofloxacin addition,showing an antagonistic effect. The complex,produced by the interaction of ciprofloxacin and copper,may be the cause of various biological effects.
The effect of soil exogenous ciprofloxacin and copper combined pollution on the early growth of wheat was studied by the toxicological diagnosis method of higher plant. The potential ecological risks of soil contamination derived from livestock manure were investigated. Results indicated the early growth indexes of wheat expressed different degrees of responses to single copper or ciprofloxacin exposure. The wheat shoot and root length were mainly inhibited,while the seed germination rate was unaffected. The sensitivities of the three indexes to the two pollutants were in order of shoot length > root length > germination rate. Ciprofloxacin and copper combined pollution showed different effects on the wheat roots and shoot length. Compared with single copper exposure,inhibition effect on wheat root length was enhanced by the combined treatment of 20mg·kg-1copper and 80 mg·kg-1ciprofloxacin,showing a synergistic effect,while the inhibition effect on wheat shoot length by the addition of 200 mg·kg-1of copper eased after ciprofloxacin addition,showing an antagonistic effect. The complex,produced by the interaction of ciprofloxacin and copper,may be the cause of various biological effects.
引文
程贯召.2008.铜离子胁迫对小麦种子萌发和幼苗生长的毒害(硕士学位论文).济南:山东师范大学.
高柱,王小玲,刘腾云,等.2013.重金属Cu污染对苏丹草种子发芽及幼苗生长的影响.中国农学通报,29(25):199-204.
葛成军,陈秋波,俞花美,等.2008.Cd胁迫对两种热带牧草种子发芽与根伸长的抑制效应.热带作物学报,29(5):568-571.
李通,金彩霞,朱雯斐,等.2013a.环丙沙星对3种作物的毒性.生态毒理学报,8(3):442-446.
李通,金彩霞,朱雯斐,等.2013b.环丙沙星与Cu复合污染对玉米萝卜和小白菜3种作物生态毒性研究.农业环境科学学报,32(1):15-20.
林仁漳,杜文超,王晓蓉,等.2008.土壤外源Cd胁迫对小麦幼苗生长自由基代谢及抗氧化酶活性的影响.农业环境科学学报,27(1):23-29.
马驿,彭金菊,王芸,等.2013.环丙沙星对土壤微生物量碳和土壤微生物群落碳代谢多样性的影响.生态学报,33(5):1506-1512.
南春容,张海智,董双林.2004.孔石莼水溶性抽提液抑制3种海洋赤潮藻的生长.环境科学学报,24(4):702-704.
宋玉芳,许华夏,任丽萍,等.2002.土壤重金属对白菜种子发芽与根伸长抑制的生态毒性效应.环境科学,23(1):104-107.
宋玉芳,周启星,许华夏,等.2002b.菲、芘、1,2,4-三氯苯对土壤高等植物根伸长抑制的生态毒性效应.生态学报,22(11):1945-1950.
王朋,温蓓,张淑贞.2011.环丙沙星对玉米芽期抗氧化酶活性及自由基代谢的影响.环境化学,30(4):754-759.
王朋.2010.抗生素及其与重金属复合污染的玉米吸收和毒性研究(硕士学位论文).北京:中国科学院生态环境研究中心.
邬腊梅,柏连阳,孟桂元,等.2012.八种除草剂对亚麻幼根和幼芽生的影响初探.中国麻业科学,34(6):270-277.
肖秋美,王建武,唐艺龄.2012.土壤-蔬菜系统中环丙沙星的降解与生物累积特征.应用生态学报,23(10):2708-2714.
俞慎,王敏,洪有为.2011.环境介质中的抗生素及其微生物生态效应.生态学报,31(15):4437-4446.
张志良,翟伟箐.2002.植物生理学实验指导.北京:高等教育出版社.
朱云集,王晨阳,马元喜,等.1997.铜胁迫对小麦根系生长发育及生理特性的影响.麦类作物,17(5):49-51.
Adams ML,Zhao FJ,McGrath SP,et al.2004.Predictingcadmium concentrations in wheat and barley grain using soilproperties.Journal of Environmental Quality,33:532-541.
Ciniglia C,Cascone C,Giudice RL,et al.2005.Application ofmethods for assessing the geno-and cytotoxicity of triclosanto ehrenbergii.Journal of Hazardous Materials,122:227-232.
Ensenbach U,Nagel R.1997.Toxicity of binary chemical mixtures:Effects on reproduction of zebrafish(Brachydanio rerio).Archives of Environmental Contamination and Toxicology,32:204-210.
Gao ML,Zhou Q,Song WH,et al.2014.Combined effects ofoxytetracycline and Pb on earthworm(Eisenia fetida).Environmental Toxicology and Pharmacology,37:689-696.
Greene JC,Bartels GL,Warren-Hicks WJ.1998.Protocols forshort term toxicity screening of hazardous wastesites.USEnvironmental Protection Agency,29:232-240.
Huang RX,Wen B,Pei ZG,et al.2009.Accumulation,subcellular distribution and toxicity of copper in earthworm(Eisenia fetida)in the presence of ciprofloxacin.Environmental Science&Technology,43:3688-3693.
Inaba S,Takenaka C.2005.Effects of dissolved organic matteron toxicity and bioavailability of copper for lettuce sprouts.Enviroment International,31:603-608.
International Organization for Standardization(ISO).1993.Soilquality:Determination of the effects of pollutants on soilflora.Part 1:Method for the measurement of inhibition ofroot growth.China ISO:Beijing:11269-11270.
Kong WD,Zhu YG,Fu BJ,et al.2006.The veterinary antibiotic oxytetracycline and Cu influence functional diversity ofthe soil microbial community.Environmental Pollution,143:129-137.
Liu F,Ying GG,Tao R,et al.2009.Effects of six selected antibiotics on plant growth and soil microbial and enzymaticactivities.Environmental Pollution,157:1636-1642.
Stewart AR,Malley DF.1999.Effect of metal mixture(Cu,Zn,Pb and Ni)on cadmium partitioning in littoral sediments and its accumulation by the freshwater macrophyteEriocaùlon septangulàre.Environmental Toxicology&Chemistry,18:436-447.
Stine OC,Johnson JA,Keefer NA,et al.2007.Widespreaddistribution of tetracycline resistance genes in a confinedanimal feeding facility.International Journal of Antimicrobial Agents,29:348-352.
Wang ME,Zhou QX.2005.Single and joint toxicity of chlorimuronethyl,cadmium,and copper acting on wheat Triticum aestivum.Ecotoxicology and Environmental Safety,60:169-175.
Zhang Y,Cai XY,Lang XM,et al.2012.Insights into aquatictoxicities of the antibiotics oxytetracycline and ciprofloxacinin the presence of metal:Complexation versus mixture.Environmental Pollution,166:48-56.
高柱,王小玲,刘腾云,等.2013.重金属Cu污染对苏丹草种子发芽及幼苗生长的影响.中国农学通报,29(25):199-204.
葛成军,陈秋波,俞花美,等.2008.Cd胁迫对两种热带牧草种子发芽与根伸长的抑制效应.热带作物学报,29(5):568-571.
李通,金彩霞,朱雯斐,等.2013a.环丙沙星对3种作物的毒性.生态毒理学报,8(3):442-446.
李通,金彩霞,朱雯斐,等.2013b.环丙沙星与Cu复合污染对玉米萝卜和小白菜3种作物生态毒性研究.农业环境科学学报,32(1):15-20.
林仁漳,杜文超,王晓蓉,等.2008.土壤外源Cd胁迫对小麦幼苗生长自由基代谢及抗氧化酶活性的影响.农业环境科学学报,27(1):23-29.
马驿,彭金菊,王芸,等.2013.环丙沙星对土壤微生物量碳和土壤微生物群落碳代谢多样性的影响.生态学报,33(5):1506-1512.
南春容,张海智,董双林.2004.孔石莼水溶性抽提液抑制3种海洋赤潮藻的生长.环境科学学报,24(4):702-704.
宋玉芳,许华夏,任丽萍,等.2002.土壤重金属对白菜种子发芽与根伸长抑制的生态毒性效应.环境科学,23(1):104-107.
宋玉芳,周启星,许华夏,等.2002b.菲、芘、1,2,4-三氯苯对土壤高等植物根伸长抑制的生态毒性效应.生态学报,22(11):1945-1950.
王朋,温蓓,张淑贞.2011.环丙沙星对玉米芽期抗氧化酶活性及自由基代谢的影响.环境化学,30(4):754-759.
王朋.2010.抗生素及其与重金属复合污染的玉米吸收和毒性研究(硕士学位论文).北京:中国科学院生态环境研究中心.
邬腊梅,柏连阳,孟桂元,等.2012.八种除草剂对亚麻幼根和幼芽生的影响初探.中国麻业科学,34(6):270-277.
肖秋美,王建武,唐艺龄.2012.土壤-蔬菜系统中环丙沙星的降解与生物累积特征.应用生态学报,23(10):2708-2714.
俞慎,王敏,洪有为.2011.环境介质中的抗生素及其微生物生态效应.生态学报,31(15):4437-4446.
张志良,翟伟箐.2002.植物生理学实验指导.北京:高等教育出版社.
朱云集,王晨阳,马元喜,等.1997.铜胁迫对小麦根系生长发育及生理特性的影响.麦类作物,17(5):49-51.
Adams ML,Zhao FJ,McGrath SP,et al.2004.Predictingcadmium concentrations in wheat and barley grain using soilproperties.Journal of Environmental Quality,33:532-541.
Ciniglia C,Cascone C,Giudice RL,et al.2005.Application ofmethods for assessing the geno-and cytotoxicity of triclosanto ehrenbergii.Journal of Hazardous Materials,122:227-232.
Ensenbach U,Nagel R.1997.Toxicity of binary chemical mixtures:Effects on reproduction of zebrafish(Brachydanio rerio).Archives of Environmental Contamination and Toxicology,32:204-210.
Gao ML,Zhou Q,Song WH,et al.2014.Combined effects ofoxytetracycline and Pb on earthworm(Eisenia fetida).Environmental Toxicology and Pharmacology,37:689-696.
Greene JC,Bartels GL,Warren-Hicks WJ.1998.Protocols forshort term toxicity screening of hazardous wastesites.USEnvironmental Protection Agency,29:232-240.
Huang RX,Wen B,Pei ZG,et al.2009.Accumulation,subcellular distribution and toxicity of copper in earthworm(Eisenia fetida)in the presence of ciprofloxacin.Environmental Science&Technology,43:3688-3693.
Inaba S,Takenaka C.2005.Effects of dissolved organic matteron toxicity and bioavailability of copper for lettuce sprouts.Enviroment International,31:603-608.
International Organization for Standardization(ISO).1993.Soilquality:Determination of the effects of pollutants on soilflora.Part 1:Method for the measurement of inhibition ofroot growth.China ISO:Beijing:11269-11270.
Kong WD,Zhu YG,Fu BJ,et al.2006.The veterinary antibiotic oxytetracycline and Cu influence functional diversity ofthe soil microbial community.Environmental Pollution,143:129-137.
Liu F,Ying GG,Tao R,et al.2009.Effects of six selected antibiotics on plant growth and soil microbial and enzymaticactivities.Environmental Pollution,157:1636-1642.
Stewart AR,Malley DF.1999.Effect of metal mixture(Cu,Zn,Pb and Ni)on cadmium partitioning in littoral sediments and its accumulation by the freshwater macrophyteEriocaùlon septangulàre.Environmental Toxicology&Chemistry,18:436-447.
Stine OC,Johnson JA,Keefer NA,et al.2007.Widespreaddistribution of tetracycline resistance genes in a confinedanimal feeding facility.International Journal of Antimicrobial Agents,29:348-352.
Wang ME,Zhou QX.2005.Single and joint toxicity of chlorimuronethyl,cadmium,and copper acting on wheat Triticum aestivum.Ecotoxicology and Environmental Safety,60:169-175.
Zhang Y,Cai XY,Lang XM,et al.2012.Insights into aquatictoxicities of the antibiotics oxytetracycline and ciprofloxacinin the presence of metal:Complexation versus mixture.Environmental Pollution,166:48-56.