土壤-蔬菜系统中环丙沙星的降解与生物累积特征
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摘要
通过盆栽试验,研究了环丙沙星对菜心、萝卜、番茄和豆角生长的影响及其累积特征和在土壤中的降解规律.结果表明:3种不同添加浓度(5、30和75mg·kg-1)的环丙沙星处理对菜心、番茄和豆角的生物量均无显著影响,而高浓度(≥30mg·kg-1)环丙沙星处理显著抑制萝卜生长;环丙沙星在土壤中的降解随着时间的延长而逐渐变缓,豆角盆栽土壤中环丙沙星的降解半衰期(DT50)为18.3～43.9d,降解90%所需要的时间(DT90)为260～738d;土壤中的环丙沙星均能被4种蔬菜的根系吸收并转移至茎叶部分,而且吸收环丙沙星的量随着土壤中环丙沙星含量的增加而增加;菜心地下部环丙沙星的含量高于地上部;而萝卜地上部环丙沙星的含量高于地下部,番茄和豆角的叶、茎、根的环丙沙星含量均为根>茎>叶.
A pot experiment was conducted to investigate the effects of applying ciprofloxacin (CIP) on the growth of flowering Chinese cabbage (Brassica campestris),radish (Raphanus sativus),tomato (Solanum lycopersicum) and long bean (Vigna unguiculata),the bioaccumation of CIP in the vegetables,and the degradation of the antibiotics in soil.The results showed that applying 5,30 and 75 mg·kg-1 of CIP had no significant effects on the plant biomass of flowering Chinese cabbage,tomato and long bean,but the high concentration of CIP (≥30 mg·kg-1) inhibited the radish growth significantly.The degradation of CIP in soil was fast at beginning,but slowed down gradually since then.The DT50 and DT90 of CIP in long bean soil were 18.3-43.9 d and 260-738 d,respectively.The CIP could be absorbed by the roots of all the four vegetables and allocated to their aboveground parts,and the CIP residues in the vegetables in creased with the concentration of CIP in soil.The CIP concentration in flowering Chinese cabbage roots was higher than that in the cabbage aboveground parts,that in radish showed a higher concentration in aboveground parts than in roots,and the CIP concentration in tomato and long bean was in the same order of root>stem>leaf.
A pot experiment was conducted to investigate the effects of applying ciprofloxacin (CIP) on the growth of flowering Chinese cabbage (Brassica campestris),radish (Raphanus sativus),tomato (Solanum lycopersicum) and long bean (Vigna unguiculata),the bioaccumation of CIP in the vegetables,and the degradation of the antibiotics in soil.The results showed that applying 5,30 and 75 mg·kg-1 of CIP had no significant effects on the plant biomass of flowering Chinese cabbage,tomato and long bean,but the high concentration of CIP (≥30 mg·kg-1) inhibited the radish growth significantly.The degradation of CIP in soil was fast at beginning,but slowed down gradually since then.The DT50 and DT90 of CIP in long bean soil were 18.3-43.9 d and 260-738 d,respectively.The CIP could be absorbed by the roots of all the four vegetables and allocated to their aboveground parts,and the CIP residues in the vegetables in creased with the concentration of CIP in soil.The CIP concentration in flowering Chinese cabbage roots was higher than that in the cabbage aboveground parts,that in radish showed a higher concentration in aboveground parts than in roots,and the CIP concentration in tomato and long bean was in the same order of root>stem>leaf.
引文
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[11]Chen Z-L(陈杖榴).Veterinary Pharmacology.Beijing:China Agriculture Press,2007(in Chinese)
[12]Pico Y,Andreu V.Fluoroquinolones in soil:Risks and challenges.Analytical and Bioanalytical Chemistry,2007,387:1287-1299
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[14]Golet EM,Xifra I,Siegrist H,et al.Environmental exposure assessment of fiuoroquinolone antibacterial agents from sewage to soil.Environmental Science and Technol-ogy,2003,37:3243-3249
[15]Ma Y(马驿),Chen Z-L(陈杖榴).Measurement of enrofloxacin remained in hog farms and their circum-ference.Journal of Traditional Chinese Veterinary Medi-cine(中兽医医药杂志),2007(6):11-16(in Chi-nese)
[16]Tai Y-P(邰义萍).Study on Pollution Characteristics of Typical Antibiotics in Soil from Vegetable Fields of Pearl River Delta Area.PhD Thesis.Guangzhou:Jinan University,2010(in Chinese)
[17]Lillenberg M,Litvin SV,Nei L,et al.Enrofloxacin and ciprofloxacin uptake by plants from soil.Agronomy Re-search,2010,8:807-814
[18]Li Y-W(李彦文),Zhang Y(张艳),Mo C-H(莫测辉),et al.Preliminary study on occurrence and health risk assessment of quinolone antibiotics in vegeta-bles from Guangzhou,China.Environmental Science(环境科学),2010,31(10):2445-2449(in Chi-nese)
[19]Zhao L,Dong YH,Wang H.Residues of veterinary antibiotics in manures from feedlot livestock in eight prov-inces of China.Science of the Total Environment,2010,408:1069-1075
[20]Zhang Y(张艳),Li Y-W(李彦文),Mo C-H(莫测辉),et al.Determination of quinolones antibiotics in vegetable using high performance liquid chromatography-fluorescence.Guangdong Agricultural Sciences(广东农业科学),2009(6):176-180(in Chinese)
[21]Tai Y-P(邰义萍),Mo C-H(莫测辉),Li Y-W(李彦文),et al.Concentration and distribution of quinolo-ne antibiotics in long-term manure-amended soils.Chi-na Environmental Science(中国环境科学),2010,30(6):816-821(in Chinese)
[22]Eggen T,Asp TN,Grave K,et al.Uptake and translocation of metformin,ciprofloxacin and narasin in forage and crop plants.Chemosphere,2011,85:26-33
[23]Wu Y-B(吴银宝),Liao X-D(廖新俤),Wang Z-S(汪植三),et al.Hydrolysis characteristics of enroflox-acin.Chinese Journal of Applied Ecology(应用生态学报),2006,17(6):1086-1090(in Chinese)
[24]Thoma K,Kuebler N.Photodegradation of quinolones.Pharmazie,1997,52:519-529
[25]Li X(李霞),Chen J-F(陈菊芳),Nie X-P(聂湘平),et al.Degradation and residue of ciprofloxacin in different simulated water bodies.Acta Scientiarum Natu-ralium Universitatis Sunyatseni(中山大学学报·自然科学版),2010,49(3):102-106(in Chinese)
[26]Vasudevan D,Bruland GL,Torrance BS,et al.pH dependent ciprofixacin sorption to soils:Interaction mech-anisms and soil factors influencing sorption.Geoderma,2009,151:68-76
[27]Uslu MO,Yediler A,Balcioglu IA,et al.Analysis and sorption behavior of fiuoroquinolones in solid matrices.Water,Air,and Soil Pollution,2008,190:55-63
[28]Tolls J.Sorption of veterinary pharmaceuticals in soils:A review.Environmental Science and Technology,2001,35:3397-3406
[29]Wang J-L(王加龙),Liu J-Z(刘坚真),Chen Z-L(陈杖榴),et al.Effect of enrofloxacin residue on number and community function diversity of soil mi-crobes.Chinese Journal of Applied and Environmental Biology(应用与环境生物学报),2005,11(1):86-89(in Chinese)
[2]LüL(吕亮),Zhang J(张静),Jiang H(姜浩).Progress on contamination of veterinary antibi-otics in soils.Environmental Science and Technology(环境科技),2010,23(2):68-70(in Chinese)
[3]Kay P,Blackwell PA,Boxall ABA.Fate of veterinary antibiotics in a macroporous tile drained clay soil.Envi-ronmental Toxicology and Chemistry,2004,23:1136-1144
[4]Halling-Sorensen B,Jacobsen AM,Jensen J,et al.Dissipation and effects of chlortetracycline and tylosin in two agricultural soils:A field-scale study in southern Denmark.Environmental Toxicology and Chemistry,2005,24:802-810
[5]Zuccato E,Castiglioni S,Bagnati R,et al.Source,oc-currence and fate of antibiotics in the Italian aquatic en-vironment.Journal of Hazardous Materials,2010,179:1042-1048
[6]Campagnolo ER,Johnson KR,Karpati A,et al.Anti-microbial residues in animal waste and water resources proximal to large-scale swine and poultry feeding opera-tions.Science of the Total Environment,2002,299:89-95
[7]Lalumera GM,Calamari D,Galli P,et al.Preliminary investigation on the environmental occurrence and effects of antibiotics used in aquaculture in Italy.Chemosphere,2004,54:661-668
[8]Kim SC,Carlson K.Temporal and spatial trends in the occurrence of human and veterinary antibiotics in aque-ous and river sediment matrices.Environmental Science and Technology,2007,41:50-57
[9]Halling-Srensen B,Nors Nielsen S,Lanzky PF,et al.Occurrence,fate and effects of pharmaceutical sub-stances in the environment:A review.Chemosphere,1998,36:357-393
[10]Sarmah AK,Meyer MT,Boxall ABA.A global perspective on the use,sales,exposure pathways occurrence,fate and effects of veterinary antibiotics(VAs)in the environment.Chemosphere,2006,65:725-759
[11]Chen Z-L(陈杖榴).Veterinary Pharmacology.Beijing:China Agriculture Press,2007(in Chinese)
[12]Pico Y,Andreu V.Fluoroquinolones in soil:Risks and challenges.Analytical and Bioanalytical Chemistry,2007,387:1287-1299
[13]Martínez-Carballo E,Gonzlez-Barreiro C,Scharf S,et al.Environmental monitoring study of selected veterina-ry antibiotics in animal manure and soils in Austria.En-vironmental Pollution,148:570-579
[14]Golet EM,Xifra I,Siegrist H,et al.Environmental exposure assessment of fiuoroquinolone antibacterial agents from sewage to soil.Environmental Science and Technol-ogy,2003,37:3243-3249
[15]Ma Y(马驿),Chen Z-L(陈杖榴).Measurement of enrofloxacin remained in hog farms and their circum-ference.Journal of Traditional Chinese Veterinary Medi-cine(中兽医医药杂志),2007(6):11-16(in Chi-nese)
[16]Tai Y-P(邰义萍).Study on Pollution Characteristics of Typical Antibiotics in Soil from Vegetable Fields of Pearl River Delta Area.PhD Thesis.Guangzhou:Jinan University,2010(in Chinese)
[17]Lillenberg M,Litvin SV,Nei L,et al.Enrofloxacin and ciprofloxacin uptake by plants from soil.Agronomy Re-search,2010,8:807-814
[18]Li Y-W(李彦文),Zhang Y(张艳),Mo C-H(莫测辉),et al.Preliminary study on occurrence and health risk assessment of quinolone antibiotics in vegeta-bles from Guangzhou,China.Environmental Science(环境科学),2010,31(10):2445-2449(in Chi-nese)
[19]Zhao L,Dong YH,Wang H.Residues of veterinary antibiotics in manures from feedlot livestock in eight prov-inces of China.Science of the Total Environment,2010,408:1069-1075
[20]Zhang Y(张艳),Li Y-W(李彦文),Mo C-H(莫测辉),et al.Determination of quinolones antibiotics in vegetable using high performance liquid chromatography-fluorescence.Guangdong Agricultural Sciences(广东农业科学),2009(6):176-180(in Chinese)
[21]Tai Y-P(邰义萍),Mo C-H(莫测辉),Li Y-W(李彦文),et al.Concentration and distribution of quinolo-ne antibiotics in long-term manure-amended soils.Chi-na Environmental Science(中国环境科学),2010,30(6):816-821(in Chinese)
[22]Eggen T,Asp TN,Grave K,et al.Uptake and translocation of metformin,ciprofloxacin and narasin in forage and crop plants.Chemosphere,2011,85:26-33
[23]Wu Y-B(吴银宝),Liao X-D(廖新俤),Wang Z-S(汪植三),et al.Hydrolysis characteristics of enroflox-acin.Chinese Journal of Applied Ecology(应用生态学报),2006,17(6):1086-1090(in Chinese)
[24]Thoma K,Kuebler N.Photodegradation of quinolones.Pharmazie,1997,52:519-529
[25]Li X(李霞),Chen J-F(陈菊芳),Nie X-P(聂湘平),et al.Degradation and residue of ciprofloxacin in different simulated water bodies.Acta Scientiarum Natu-ralium Universitatis Sunyatseni(中山大学学报·自然科学版),2010,49(3):102-106(in Chinese)
[26]Vasudevan D,Bruland GL,Torrance BS,et al.pH dependent ciprofixacin sorption to soils:Interaction mech-anisms and soil factors influencing sorption.Geoderma,2009,151:68-76
[27]Uslu MO,Yediler A,Balcioglu IA,et al.Analysis and sorption behavior of fiuoroquinolones in solid matrices.Water,Air,and Soil Pollution,2008,190:55-63
[28]Tolls J.Sorption of veterinary pharmaceuticals in soils:A review.Environmental Science and Technology,2001,35:3397-3406
[29]Wang J-L(王加龙),Liu J-Z(刘坚真),Chen Z-L(陈杖榴),et al.Effect of enrofloxacin residue on number and community function diversity of soil mi-crobes.Chinese Journal of Applied and Environmental Biology(应用与环境生物学报),2005,11(1):86-89(in Chinese)