去整合素样金属蛋白酶8在结肠癌中检测的临床意义研究
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摘要
[目的]检测结肠癌组织中去整合素样金属蛋白酶8(ADAM8)基因表达以及患者外周血中ADAM8蛋白表达的变化,探讨了其相关的临床意义。[方法]收集2017年2月～2018年10月我院普外科治疗的结肠癌患者82例,另取我院体检中心健康体检者30例作为正常健康对照。ELISA法检测外周血中ADAM8蛋白表达水平。荧光定量PCR检测ADAM8基因表达水平。[结果]结肠癌患者癌组织中ADAM8基因的2~(-△△Ct)值为0.38±0.09,显著高于癌旁组织的0.16±0.04(P<0.01)。结肠癌患者外周血ADAM8蛋白表达水平为(22.35±5.14)μg/L,显著高于对照组的(8.07±0.15)μg/L(P<0.01)。结肠癌患者癌组织中ADAM8基因2~(-△△Ct)值和蛋白表达水平在肿瘤大小≥5 cm患者为(0.44±0.12)和(25.24±5.65)μg/L,显著高于<5 cm患者的(0.31±0.06)和(18.32±4.52)μg/L(P<0.05),在Ⅲ、Ⅳ期患者为(0.43±0.11)和(25.50±5.76)μg/L,显著高于Ⅰ、Ⅱ期患者的(0.32±0.07)和(19.16±4.63)μg/L(P<0.05),在淋巴结有转移患者为(0.45±0.14)和(25.20±5.83)μg/L,显著高于无转移患者的(0.25±0.04)和(16.86±4.45)μg/L(P<0.01)。[结论]结肠癌患者肿瘤组织和外周血中ADAM8高表达,高表达的ADAM8可能参与了结肠癌的发生、侵袭和转移过程。
[Objective] The expression of ADAM 8 gene and the expression of ADAM 8 protein in peripheral blood of patients with colon cancer were detected, and its clinical significance was discussed.[Methods]82 patients of colon cancer were enrolled in this study. Another 30 healthy people were used as controls. The protein expression of ADAM8 in peripheral blood was detected by ELISA analysis. The mRNA expression of ADAM8 was detected by Real-time PCR analysis. [Results]The 2~(-△△Ct) value of ADAM 8 in colon cancer tissue was 0.38±0.09, which was higher than that of 0.16±0.04 in paracancerous tissue(P<0.01). The ADAM 8 protein expression in blood of colon cancer patient was(22.35±5.14) μg/L, which was higher than that of(8.07±0.15) μg/L in control group(P<0.01). The 2~(-△△Ct) value and protein expression of ADAM 8 in tumor size ≥ 5 cm was(0.44±0.12) and(25.24±5.65) μg/L, which was higher than that of(0.31±0.06) and(18.32±4.52) μg/L in in tumor size < 5 cm(P<0.05), patients in Ⅲ and Ⅳ stage were(0.43±0.11)and(25.50±5.76) μg/L, which were higher those in stage Ⅰ and Ⅱ(0.32±0.07) and(19.16±4.63) μg/L(P<0.05).(P<0.05), patients with metastatic lymph nodes were(0.45±0.14)and(25.20±5.83) μg/L, which was higher than that of(0.25±0.04) and(16.86±4.45) μg/L in non-lymph node metastasis(P<0.01). [Conclusion]ADAM8 is highly expressed in tumor tissues and peripheral blood of patients with colon cancer, and high expression of ADAM 8 may be involved in the occurrence, invasion and metastasis of colon cancer.
[Objective] The expression of ADAM 8 gene and the expression of ADAM 8 protein in peripheral blood of patients with colon cancer were detected, and its clinical significance was discussed.[Methods]82 patients of colon cancer were enrolled in this study. Another 30 healthy people were used as controls. The protein expression of ADAM8 in peripheral blood was detected by ELISA analysis. The mRNA expression of ADAM8 was detected by Real-time PCR analysis. [Results]The 2~(-△△Ct) value of ADAM 8 in colon cancer tissue was 0.38±0.09, which was higher than that of 0.16±0.04 in paracancerous tissue(P<0.01). The ADAM 8 protein expression in blood of colon cancer patient was(22.35±5.14) μg/L, which was higher than that of(8.07±0.15) μg/L in control group(P<0.01). The 2~(-△△Ct) value and protein expression of ADAM 8 in tumor size ≥ 5 cm was(0.44±0.12) and(25.24±5.65) μg/L, which was higher than that of(0.31±0.06) and(18.32±4.52) μg/L in in tumor size < 5 cm(P<0.05), patients in Ⅲ and Ⅳ stage were(0.43±0.11)and(25.50±5.76) μg/L, which were higher those in stage Ⅰ and Ⅱ(0.32±0.07) and(19.16±4.63) μg/L(P<0.05).(P<0.05), patients with metastatic lymph nodes were(0.45±0.14)and(25.20±5.83) μg/L, which was higher than that of(0.25±0.04) and(16.86±4.45) μg/L in non-lymph node metastasis(P<0.01). [Conclusion]ADAM8 is highly expressed in tumor tissues and peripheral blood of patients with colon cancer, and high expression of ADAM 8 may be involved in the occurrence, invasion and metastasis of colon cancer.
引文
[1] 周欣,李晖,徐彩虹,等.结直肠癌组织中Ki-67和pAKT的表达及其临床意义[J].中国中西医结合消化杂志,2019,27(2):122-127.
[2] Conrad C,Gotte M,Schlomann U,et al.ADAM8 expression in breast cancer derived brain metastases:Functional implications on MMP-9 expression and transendothelial migration in breast cancer cells[J].Int J Cancer,2018,142(4):779-791.
[3] 刘亚龙,段彪,张锐东,等.去整合素-基质金属蛋白酶在肿瘤细胞中的作用研究进展[J].医学综述,2016,22(4):733-736.
[4] 胡以利,望晓波,陈点点.Vav1、ADAM8对胃癌病灶内癌细胞增殖及侵袭的调节作用[J].海南医学院学报,2018,24(3):373-375,379.
[5] 刘伟,杨鹏,李于红,等.ILK及E-cadherin在结肠癌中的表达及其预后意义[J].中国中西医结合消化杂志,2017,25(8):574-578.
[6] 白杨,陈浩,李纪政,等.ADAM8基因调控AKT/mTOR通路对结肠癌细胞HCT8的影响[J].中国生化药物杂志,2014,34(3):24-27.
[7] 张韶岩,曾小莉,区颂雷,等.肺癌患者血清骨桥蛋白与解整合素-金属蛋白酶8表达及意义[J].中华实用诊断与治疗杂志,2017,31(7):665-667.
[8] Das SG,Romagnoli M,Mineva ND,et al.miR-720 is a downstream target of an ADAM8-induced ERK signaling cascade that promotes the migratory and invasive phenotype of triple-negative breast cancer cells[J].Breast Cancer Res,2016,18(1):40-48.
[9] Yang Z,Bai Y,Huo L,et al.Expression of A disintegrin and metalloprotease 8 is associated with cell growth and poor survival in colorectal cancer[J].BMC Cancer,2014,14(2):568-572.
[10] 何楠,金倩娜,黄永明,等.ADAM8基因在胃癌组织中的表达及与临床病理特征的关系[J].华中科技大学学报(医学版),2017,46(1):1-4.
[2] Conrad C,Gotte M,Schlomann U,et al.ADAM8 expression in breast cancer derived brain metastases:Functional implications on MMP-9 expression and transendothelial migration in breast cancer cells[J].Int J Cancer,2018,142(4):779-791.
[3] 刘亚龙,段彪,张锐东,等.去整合素-基质金属蛋白酶在肿瘤细胞中的作用研究进展[J].医学综述,2016,22(4):733-736.
[4] 胡以利,望晓波,陈点点.Vav1、ADAM8对胃癌病灶内癌细胞增殖及侵袭的调节作用[J].海南医学院学报,2018,24(3):373-375,379.
[5] 刘伟,杨鹏,李于红,等.ILK及E-cadherin在结肠癌中的表达及其预后意义[J].中国中西医结合消化杂志,2017,25(8):574-578.
[6] 白杨,陈浩,李纪政,等.ADAM8基因调控AKT/mTOR通路对结肠癌细胞HCT8的影响[J].中国生化药物杂志,2014,34(3):24-27.
[7] 张韶岩,曾小莉,区颂雷,等.肺癌患者血清骨桥蛋白与解整合素-金属蛋白酶8表达及意义[J].中华实用诊断与治疗杂志,2017,31(7):665-667.
[8] Das SG,Romagnoli M,Mineva ND,et al.miR-720 is a downstream target of an ADAM8-induced ERK signaling cascade that promotes the migratory and invasive phenotype of triple-negative breast cancer cells[J].Breast Cancer Res,2016,18(1):40-48.
[9] Yang Z,Bai Y,Huo L,et al.Expression of A disintegrin and metalloprotease 8 is associated with cell growth and poor survival in colorectal cancer[J].BMC Cancer,2014,14(2):568-572.
[10] 何楠,金倩娜,黄永明,等.ADAM8基因在胃癌组织中的表达及与临床病理特征的关系[J].华中科技大学学报(医学版),2017,46(1):1-4.