miRNA在稳定白斑和癌变白斑组织中表达的比较
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摘要
目的通过分析癌变白斑和稳定白斑病损组织中miRNA表达谱的异常,以期发现可准确评估此类病损癌变风险的生物学标志物。方法使用Taq Man低密度miRNA芯片对稳定白斑和癌变白斑进行分子生物学检测分析。结果同稳定白斑相比较,癌变白斑中miRNAs的表达发生了显著改变,差异有统计学意义(P<0.05),其中13个miRNAs的表达下调(miR-99a-5p、miR-99b-5p、miR-144、miR-100-5p、miR-125-5p、miR-181b、miR-181a、miR-521-3p、miR-339-5p、miR-15a-5p、miR-150-5p、let-7a-5p、miR-9),12个miRNAs的表达上调(miR-10b-5p、miR-708、miR-30e-3p、miR-30a-3p、miR-21、miR-335-5p、miR-144、miR-25-3p、miR-19a-3p、miR-660-5p、miR-140-5p、miR-590-5p)。结论 miRNA生物标志物的检测对稳定白斑和癌变白斑病变的诊断具有重要的意义。
Objective To assess the potential of a progress risk of oral precancerous lesions through the analysis of abnormal expression of miRNA. Methods Used miRNA microarray to profile progressing low grade dysplasia and oral premaligant lesions,which was in order to do molecular biology detection analysis. Results Compared with low grade dysplasia,oral premaligant lesions had a significant change with express of miRNA( P < 0. 05). Leukoplakias,with up-regulations of miR-99a-5p,miR-99b-5p,miR-144,miR-100-5p,miR-125-5p、miR-181b、miR-181a、miR-521-3p、miR-339-5p、miR-15a-5p,miR-150-5p,let-7a-5p,miR-9,and down-regulations of miR-10b-5p,miR-708,miR-30e-3p,miR-30a-3p,miR-21,miR-335-5p,miR-144,miR-25-3p,miR-19a-3p,miR-660-5p,miR-140-5p,miR-590-5p. Conclusions It is of great significance with diagnose of low grade dysplasia and oral premaligant lesions by miRNA biomarker detection.
Objective To assess the potential of a progress risk of oral precancerous lesions through the analysis of abnormal expression of miRNA. Methods Used miRNA microarray to profile progressing low grade dysplasia and oral premaligant lesions,which was in order to do molecular biology detection analysis. Results Compared with low grade dysplasia,oral premaligant lesions had a significant change with express of miRNA( P < 0. 05). Leukoplakias,with up-regulations of miR-99a-5p,miR-99b-5p,miR-144,miR-100-5p,miR-125-5p、miR-181b、miR-181a、miR-521-3p、miR-339-5p、miR-15a-5p,miR-150-5p,let-7a-5p,miR-9,and down-regulations of miR-10b-5p,miR-708,miR-30e-3p,miR-30a-3p,miR-21,miR-335-5p,miR-144,miR-25-3p,miR-19a-3p,miR-660-5p,miR-140-5p,miR-590-5p. Conclusions It is of great significance with diagnose of low grade dysplasia and oral premaligant lesions by miRNA biomarker detection.
引文
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[2]Fabian MR,Sonenberg N,Filipowicz W.Regulation of mRNA Translation and Stability by microRNAs[J].Annu Rev Biochem,2010,79:351-379.
[3]Portela A,Esteller M.Epigenetic modifications and human disease[J].Nature Biotechnol,2010,28(10):1057-1068.
[4]Wiklund ED,Gao S,Hulf T,et al.MicroRNA alterations and associated aberrant DNA methylation patterns across multiple sample types in oral squamous cell carcinoma[J].PLo S One,2011,6(11):e27840.
[5]lliopoulos D,Jaeger SA,Hirsch HA,et al.STAT3 activation of miR-21 and miR-181b-1 via PTEN and CYLD are part of the epigenetic switch linking inflammation to cancer[J].Mol Cell,2010,39(4):493-506.
[6]Cervigne NK,Reis PP,Machado J,et al.Identification of a microRNA signature associated with progression of leukoplakia to oral carcinoma[J].Hum Mol Genet,2009,18(24):4818-4829.
[7]Park NJ,Zhou H,Elashoff D,et al.Salivary microRNA:discovery,characterization,and clinical utility for oral cancer detection[J].Clin Cancer Res,2009,15(17):5473-5477.
[8]Etheridge A,Lee I,Hood L,et al.Extracellular microRNA:A new source of biomarkers[J].Mutat Res,2011,717(1/2):85-90.