白兔模型中肛门病术后创面边缘形态对愈合速度的影响研究
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摘要
目的观察创面边缘形态对肛门病术后白兔模型创面愈合的影响。方法制备肛门病术后创面的白兔模型,将创缘与创面呈角为90~°的创面归入"直切组",将创缘与创面呈角为135~°的创面归入"斜切组"。分别于造模后第3 d、7 d、10 d、14 d、18 d切取创面肉芽组织进行分析,观察病理变化。结果在造模后第7 d、10 d、14 d,斜切组创面闭合指数均高于直切组,差异均有统计学意义(均P<0.05)。在造模后第3 d、7 d,斜切组创面的毛细血管数量及成纤维细胞数量均较直切组多,差异均有统计学意义(均P<0.05)。在造模后第7 d、10 d、14 d,斜切组创面中的炎性细胞情况大体优于同期直切组。结论对于肛门病术后创面而言,改变肛门部创面边缘的形态(将创缘与创面角度由90~°增加到135~°),可以减轻创面炎性反应,加速创面的愈合。
Objective To study the influence of wound edge shape on healing after anal operation in a rabbit model. Methods The model was built in albino rabbits. The angle of incision in the vertical incision group is 90~° between the wound surface and edge,and the angle in the oblique incision group is 135~°. The granulation tissue was analyzed on the 3 rd, 7 th, 10 th, 14 th, 18 thdays after modeling and the pathological changes was observed. Results On the 7 th, 10 th, and14 thday after modeling, the wound-closed index was higher in the oblique incision group than in the vertical incision group(all P < 0.05). On the 3 rdand 7 thday after modeling, there were more blood capillaries and mechanocytes in the wound in the oblique incision group than in the vertical incision group(all P <0.05). On the 7 th, 10 th, and 14 thday after modeling, the situation of inflammatory cells in the oblique incision group was in general better than in the vertical incision group. Conclusion Changing the wound edge shape by changing the angle between the sound surface and edge from 90~° to 135~° could relieve inflammatory reaction and promote wound healing.
Objective To study the influence of wound edge shape on healing after anal operation in a rabbit model. Methods The model was built in albino rabbits. The angle of incision in the vertical incision group is 90~° between the wound surface and edge,and the angle in the oblique incision group is 135~°. The granulation tissue was analyzed on the 3 rd, 7 th, 10 th, 14 th, 18 thdays after modeling and the pathological changes was observed. Results On the 7 th, 10 th, and14 thday after modeling, the wound-closed index was higher in the oblique incision group than in the vertical incision group(all P < 0.05). On the 3 rdand 7 thday after modeling, there were more blood capillaries and mechanocytes in the wound in the oblique incision group than in the vertical incision group(all P <0.05). On the 7 th, 10 th, and 14 thday after modeling, the situation of inflammatory cells in the oblique incision group was in general better than in the vertical incision group. Conclusion Changing the wound edge shape by changing the angle between the sound surface and edge from 90~° to 135~° could relieve inflammatory reaction and promote wound healing.
引文
[1]罗京艺,李树亮,王敏.肛愈宁软膏对肛门病术后模型创面Ⅰ型和Ⅲ型胶原表达的影响[J].世界中西医结合杂志,2015,10(10):1361-1363.
[2]袁学刚,王战国,邹亮,等.连栀矾溶液促进大鼠肛周脓肿创面愈合作用研究[J].成都中医药大学学报,2016,39(1):39-43.
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[4]周宗科,裴福兴,程惊秋,等.不同方法处理对同种异体皮质骨板移植组织相容性的影响[J].华西医学,2005,20(1):30.
[5]张双双,郑美春,黄漫容,等.伤口床不健康肉芽组织护理研究进展[J].中国实用护理杂志,2016,32(13):1038-1040.
[6]陈敏,黄德铨,杜勇军等.熊珍膏干预大鼠皮肤创面新生肉芽组织成纤维细胞及新生毛细血管影响的实验研究[J].时珍国医国药,2012,23(6):1061-1063.
[7]施高强,贾赤字.论成纤维细胞在创面愈合中作用的认识历程[J].医学与哲学,2013,34(7):81-84.
[8]郑旺,肖志波,王瑜.老年患者难愈创面的研究进展[J].中国老年学杂志,2013,36(1):210-213.
[9]林材,罗旭.细胞缺氧与创面难愈的相关性思考[J].中华损伤与修复杂志(电子版),2010,5(3):298-301.
[10]钟天昌.几种难愈创面的发生原因与发病机制的研究[J].医药前沿,2012,2(4):140-141.
[11]张乳艳,令狐庆.仙方活命饮对大鼠肛周脓肿创面愈合实验研究[J].辽宁中医药大学学报.2013,15(6):229-231.
[12]顾红,陈红锦,陈超钟.透脓散对肛周脓肿术后模型创面愈合的实验研究[J].四川中医,2014,32(1):81-83.
[13]袁学刚,王战国,邹亮.连栀矾溶液促进大鼠肛周脓肿创面愈合作用研究[J].成都中医药大学学报,2016,39(1):39-43.
[2]袁学刚,王战国,邹亮,等.连栀矾溶液促进大鼠肛周脓肿创面愈合作用研究[J].成都中医药大学学报,2016,39(1):39-43.
[3]罗京艺,尚毅,王敏,朱东华.肛愈宁软膏对大鼠脓肿创面愈合的促进作用[J].中国中西医结合外科杂志,2011,17(3):283-286.
[4]周宗科,裴福兴,程惊秋,等.不同方法处理对同种异体皮质骨板移植组织相容性的影响[J].华西医学,2005,20(1):30.
[5]张双双,郑美春,黄漫容,等.伤口床不健康肉芽组织护理研究进展[J].中国实用护理杂志,2016,32(13):1038-1040.
[6]陈敏,黄德铨,杜勇军等.熊珍膏干预大鼠皮肤创面新生肉芽组织成纤维细胞及新生毛细血管影响的实验研究[J].时珍国医国药,2012,23(6):1061-1063.
[7]施高强,贾赤字.论成纤维细胞在创面愈合中作用的认识历程[J].医学与哲学,2013,34(7):81-84.
[8]郑旺,肖志波,王瑜.老年患者难愈创面的研究进展[J].中国老年学杂志,2013,36(1):210-213.
[9]林材,罗旭.细胞缺氧与创面难愈的相关性思考[J].中华损伤与修复杂志(电子版),2010,5(3):298-301.
[10]钟天昌.几种难愈创面的发生原因与发病机制的研究[J].医药前沿,2012,2(4):140-141.
[11]张乳艳,令狐庆.仙方活命饮对大鼠肛周脓肿创面愈合实验研究[J].辽宁中医药大学学报.2013,15(6):229-231.
[12]顾红,陈红锦,陈超钟.透脓散对肛周脓肿术后模型创面愈合的实验研究[J].四川中医,2014,32(1):81-83.
[13]袁学刚,王战国,邹亮.连栀矾溶液促进大鼠肛周脓肿创面愈合作用研究[J].成都中医药大学学报,2016,39(1):39-43.