高效氯氰菊酯对中华绒螯60 d慢性毒理及病理研究
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摘要
为研究高效氯氰菊酯对中华绒螯扣蟹生长发育的影响,该实验采取急性毒性试验方式,以60d为记录周期,定时测定其体质量与存活率。试验结果表明,中华绒螯蟹(Eriocheir sinensis)扣蟹在高效氯氰菊酯(0.10、1.00mg/L处理组)作用下体质量明显降低。尤其是1.00mg/L浓度组最明显。0.01mg/L处理组和对照组扣蟹无明显异常现象。且在1.00mg/L浓度组下存活率较其他浓度也下降得很快。说明相比较0.10mg/L和0.01mg/L,1.00mg/L浓度的高效氯氰菊酯对中华绒螯扣蟹毒性最强。石蜡切片结果显示:一定浓度的氯氰菊酯会对河蟹的肝胰腺、鳃和肌肉组织产生明显的病理变化。
In order to study the effect of beta-cypermethrin on the growth and development of Eriocheir sinensis,the acute toxicity test method was adopted in the experiment.The body weight and survival rate were measured periodically at a cycle of 60 days.The results showed that the weight of E.sinensis crabs in the beta-cypermethrin(0.10,1.00 mg/Ltreatment group)was significantly reduced,especially in the 1.00 mg/Lconcentration group.There was no obvious abnormality in the 0.01 mg/Ltreatment group and the control group.Also,the survival rate in the 1.00 mg/Lconcentration group also decreased rapidly compared with other concentrations.It was indicated that compared with 0.10 and 0.01 mg/L,beta-cypermethrin at the concentration of 1.00 mg/Lwas the most toxic to E.sinensis.Paraffin section results showed that a certain concentration of cypermethrin produced significant pathological changes in the hepatopancreas,tendon and muscle tissue of E.sinensis.
In order to study the effect of beta-cypermethrin on the growth and development of Eriocheir sinensis,the acute toxicity test method was adopted in the experiment.The body weight and survival rate were measured periodically at a cycle of 60 days.The results showed that the weight of E.sinensis crabs in the beta-cypermethrin(0.10,1.00 mg/Ltreatment group)was significantly reduced,especially in the 1.00 mg/Lconcentration group.There was no obvious abnormality in the 0.01 mg/Ltreatment group and the control group.Also,the survival rate in the 1.00 mg/Lconcentration group also decreased rapidly compared with other concentrations.It was indicated that compared with 0.10 and 0.01 mg/L,beta-cypermethrin at the concentration of 1.00 mg/Lwas the most toxic to E.sinensis.Paraffin section results showed that a certain concentration of cypermethrin produced significant pathological changes in the hepatopancreas,tendon and muscle tissue of E.sinensis.
引文
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[4]孙小静,马利民,龚本涛.氯氰菊酯在土壤环境中的存在行为及分析研究进展[J].农药,2010,49(01):16-18.
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[6]杨先乐.从河蟹“水瘪子”谈我国水产动物病害研究的导向[J].科学养鱼,2015(10):1-1.
[7]张凤翔,顾明,张建楼.河蟹“水瘪子”病的预防措施[J].科学养鱼,2010(8):53-53.
[8]周日东,王松刚,张凤翔.河蟹水瘪子病的发生与应急措施[J].科学养鱼,2016(4).
[9]杨宗英,张一柳,刘力硕,等.2015-2016河蟹“水瘪子”病发病情况.科学养鱼,2016,(10):54-55.
[10]洪宇航.溴氰菊酯对中华绒螯蟹主要免疫指标的影响[J].广东农业科学,2017,44(03):151-157.
[11]杨宗英,张一柳,胡鲲,等.溴氰菊酯对中华绒螯蟹肝胰腺氧化胁迫效应和组织结构的影响[J].浙江农业学报,2017,29(08):1261-1270.
[12]耿雪冰,沈美芳,吴光红,等.溴氰菊酯对河蟹的急性毒性研究[J].水产养殖,2009,30(10):48-50.
[13]耿雪冰.溴氰菊酯残留的检测方法及对河蟹的毒性研究[D].南京农业大学,2010.
[14]许迪,潘竟林,刘万强,等.多杀菌素、阿维菌素乳油和高效氯氰菊酯3种农药对环境生物的安全性评价[J].生态毒理学报,2013,8(06):897-902.