企鹅珍珠贝抗氧化系统对铜暴露的响应
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摘要
在水温(27.6±1.0)℃下,容积为0.2m3玻璃钢水桶中,采用静态法研究了五水硫酸铜配制的Cu~(2+)质量浓度为10、20μg/L和40μg/L对体质量(88.24±13.32)g企鹅珍珠贝的肝胰脏组织中谷胱甘肽过氧化物酶、过氧化氢酶和超氧化物歧化酶活性的影响。结果表明,Cu~(2+)对企鹅珍珠贝抗氧化酶活性均有诱导作用;在Cu~(2+)较低质量浓度作用下,谷胱甘肽过氧化物酶活性上升较快,而过氧化氢酶活性则在较高质量浓度组活性提升显著,超氧化物歧化酶活性在不同质量浓度下早期均呈缓慢上升的趋势,高质量浓度组在试验结束时仍然保持较高活性;以上3种抗氧化酶均对重金属Cu~(2+)较敏感,可作为海水环境中Cu~(2+)含量的监测指示物。
The activities of glutathione-peroxidae(GPx),catalase(CAT)and superoxide dismutase were(SOD)were detected in hepatopancreas of pearl oyster Pteria penguin with body weight of(88.24±13.32)g exposed to Cu~(2+) concentrations of 0,10,20 or 40μg/L prepared by CuSO_4·5H_2O in 0.2 m~3 glass fiber tanks at water temperature of(27.6±1.0)℃ by static method in order to understand the effects of copper on antioxidant capacity of pearl oyster.The results showed that Cu~(2+) induced the increase in activities of the GPx,CAT and SOD,and GPx activity was found to be elevated at low concentration of Cu~(2+).However,the CAT activity was increased quickly at high Cu~(2+) concentration.In all groups,SOD activities were elevated gradually in early rearing period,and kept higher at high Cu~(2+) concentration in the end of the experiment.The findings indicate that the 3 antioxidases above are all sensitive to copper,and could be an indicator in survey of copper pollution in the sea water.
The activities of glutathione-peroxidae(GPx),catalase(CAT)and superoxide dismutase were(SOD)were detected in hepatopancreas of pearl oyster Pteria penguin with body weight of(88.24±13.32)g exposed to Cu~(2+) concentrations of 0,10,20 or 40μg/L prepared by CuSO_4·5H_2O in 0.2 m~3 glass fiber tanks at water temperature of(27.6±1.0)℃ by static method in order to understand the effects of copper on antioxidant capacity of pearl oyster.The results showed that Cu~(2+) induced the increase in activities of the GPx,CAT and SOD,and GPx activity was found to be elevated at low concentration of Cu~(2+).However,the CAT activity was increased quickly at high Cu~(2+) concentration.In all groups,SOD activities were elevated gradually in early rearing period,and kept higher at high Cu~(2+) concentration in the end of the experiment.The findings indicate that the 3 antioxidases above are all sensitive to copper,and could be an indicator in survey of copper pollution in the sea water.
引文
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[32]Geret F,Serafim A,Barreira L.Response of antioxidant systems to copper in the gills of the clam Ruditapes decussates[J].Marine Environmental Research,2002,54(3):413-417.
[33]王凡,赵元凤,吕景才,等.Cu2+对栉孔扇贝鳃组织抗氧化酶的影响[J].湖北农业科学,2007,46(1):117-119.
[2]王增焕,林钦,李刘东,等.不同海域鲍体镉分布特征与健康风险评价[J].南方水产科学,2013,9(1):22-27.
[3]薛岚.天然水体中金属污染物的来源、迁移及控制[J].宁德师专学报:自然科学版,2001,13(4):372-374.
[4]Bogdanova A Y,Gassmann M,Nikinmaa M.Copper ion redox state is critical for its effects on ion transport pathways and methaemoglobin formation in trout erythrocytes[J].Chemico-biological Interactions,2002,139(1):43-59.
[5]Gwozdziński K.Structural changes in erythrocyte components induced by copper and mercury[J].Radiation Physics and Chemistry,1995,45(6):877-882.
[6]Gonzalez P,Dominique Y,Massabuau J C,et al.Comparative effects of dietary methylmercury on gene expression in liver,skeletal muscle,and brain of the zebrafish(Danio rerio)[J].Environmental Science and Technology,2005,39(11):3972-3980.
[7]蔡文超,区又君.重金属离子铜对鱼类早期发育阶段的毒性[J].南方水产科学,2009,5(5):75-79.
[8]张晨捷,张艳亮,高权新,等.低盐胁迫对黄姑鱼幼鱼肝脏抗氧化功能的影响[J].南方水产科学,2015,11(4):59-64.
[9]李有宁,陈明强,严俊贤,等.我国热带海水大型珍珠贝类的珍珠养殖技术现状及对策建议[J].广东农业科学,2013(1):136-138.
[10]庞艳华,姜伟,薛大方,等.大连沿海虾夷扇贝体内重金属含量研究与质量评价[J].水产科学,2012,31(3):156-159.
[11]Rainbow P S.Biomonitoring of heavy metal availability in the marine environment[J].Marine Pollution Bulletin,1995,31(4):183-192.
[12]Wang W X,Fisher N S,Lucma S N.Kinetic determinations of trace element bioaccumulation in the mussel Mytilus edulis[J].Marine Ecoloyg-Progress Series,1996,140(1/3):91-113.
[13]励建荣,李学鹏,王丽,等.贝类对重金属的吸收转运与累积规律研究进展[J].水产科学,2007,26(1):51-55.
[14]刘世良,麦康森.贝类免疫系统和机理的研究进展[J].海洋学报,2003,25(2):95-105.
[15]刘浩明,董迎辉,霍礼辉,等.Cu2+对缢蛏稚贝的急性毒性及对抗氧化酶活力和丙二醛含量的影响[J].中国水产科学,2012,19(1):182-187.
[16]陈琳琳,张高生,陈静,等.汞、硒暴露对紫贻贝(Mytilus edulis)抗氧化酶系统的影响[J].生态毒理学报,2011,6(4):383-388.
[17]王清.几种重金属和有机污染物对文蛤Meretrxi meretrxi生态毒理效应的研究[D].青岛:中国科学院海洋研究所,2010.
[18]Rui C,Angela S,Richard C,et al.The effect of cadmium on antioxidant responses and the susceptibility to oxidative stress in the hydrothermal vent mussel Bathymodiolus azoricus[J].Marine Biology,2006,148(4):817-825.
[19]Cantúmedellín N,OlguínMonroy N O,Méndez-rodríguez L C,et al.Antioxidant enzymes and heavy metal levels in tissues of the black chocolate clam Megapitaria squalidain Bahía de La Paz,Mexico[J].Archives of Environmental Contamination and Toxicology,2009,56(1):60-66.
[20]Cong M,Wu H F,Liu X L,et al.Effects of heavy metals on the expression of a zinc-inducible metallothionein-Ⅲgene and antioxidant enzyme activities in Crassostrea gigas[J].Ecotoxicology,2012,21(7):1928-1936.
[21]Geret F,Serafim A,Barreira L,et al.Response of antioxidant systems to copper in the gills of the clam Ruditapes decussatus[J].Marine Environmental Research,2002,54(3/5):413-417.
[22]Winston G W,DiGiulio R T.Prooxidant and antioxidant mechanisms in aquatic organisms[J].Aquatic Toxicology,1991,19(2):137-161.
[23]Lesser M P.Oxidative stress in marine environments:biochemistry and physiological ecology[J].Annual Review of Physiology,2006,68(1):253-278.
[24]McCord J M,Fridovich I.Superoxide dismutase:an enzymic function for erythrocuprein(hemocuprein)[J].Journal of Biological Chemistry,1969,244(22):6049-6055.
[25]Jones D P,Ekiow L,Thor H,et al.Metabolism of hydrogen peroxide in isolated hepatocytes:relative contributions of catalase and glutathione peroxidase in decomposition of endogenously generated H2O2[J].Archives of Biochemistry&Biophysics,1981,210(2):505-516.
[26]张林宝,吴惠丰,孙伟,等.菲律宾蛤仔对镉、铜暴露的蓄积作用及其抗氧化酶系统的响应研究[J].南方水产科学,2013,9(5):64-70.
[27]FlohéL.Glutathione peroxidase[J].Basic Life Sciences,2013,49(5):399-404.
[28]高层.重金属Pb2+和Cu2+对太平洋牡蛎(Crassostrea gigas)毒性效应的比较研究[D].青岛:中国海洋大学,2012.
[29]Winston G W.Oxidants and antioxidants in aquatic animals[J].Comp Biochem Physiol,1991,100(1/2):173-176.
[30]Parke V D.Role of enzymes in protection against lipid peroxidation[J].Regul Toxicol Pharmacol,1987(7):222-235.
[31]Basha P S,Rani A U.Cadmium-induced antioxidant defense mechanism in freshwater teleost Oreochromis mossambicus(tilapia)[J].Ecotoxicology and Environmental Safety,2003,56(2):218-221.
[32]Geret F,Serafim A,Barreira L.Response of antioxidant systems to copper in the gills of the clam Ruditapes decussates[J].Marine Environmental Research,2002,54(3):413-417.
[33]王凡,赵元凤,吕景才,等.Cu2+对栉孔扇贝鳃组织抗氧化酶的影响[J].湖北农业科学,2007,46(1):117-119.