大鼠供肝保存时TET对HCA液的优化作用及与UW液的保存效果比较
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摘要
目的通过用不同的保存液低温灌洗保存大鼠供肝2 h的实验,比较汉防己甲素(TET)-高渗枸橼酸盐嘌呤(HCA)溶液与UW(University of Wisconsin)液对供肝的保护作用,并分析TET对HCA液的优化效果。方法 SD大鼠80只,随机平均分成4组:A组(TET乳酸林格液组)、B组(HCA液组)、C组(TET-HCA液组)、D组(UW液组)。经实验处理后再检测各组肝组织的高能磷酸化合物的含量及保存液中丙氨酸氨基转移酶(ALT)、乳酸脱氢酶(LDH)值。结果 A组与B组各指标比较无显著性差异(P>0.05);C组与B组各指标比较无显著性差异(P>0.05);C组与D组各指标比较具有显著性差异(P<0.05)。结论在低温灌洗保存供肝条件下TET对HCA液具有积极的优化作用;TET-HCA液低温灌洗并保存鼠肝2 h,对供肝的保护作用具有与UW液类同的效果。
Objective To analyse the optimization effect on HCA of TET by comparing the preservation validity of TET-HCA and UW solutions on donor liver for 2 hrs perfused with different solutions.Methods 80 SD rats were divided into 4 groups perfused by TET-Ringer(A),TET-HCA(B),HCA(C) and UW(D)solutions,respectively.ATP in hepatocytes and ALT,LDH in preserved solutions were monitored and pathological changes of the preserved liver were observed after experiment.Results No significant changes were observed between group A and B.Instead,there are significant changes between group B and C,so were in groups C and D.Conclusion TET-HCA has optimization effect on HCA under the cold preservation on donor liver.During 2 hrs preservation period,similar effect was found between TET-HCA and UW solutions.
Objective To analyse the optimization effect on HCA of TET by comparing the preservation validity of TET-HCA and UW solutions on donor liver for 2 hrs perfused with different solutions.Methods 80 SD rats were divided into 4 groups perfused by TET-Ringer(A),TET-HCA(B),HCA(C) and UW(D)solutions,respectively.ATP in hepatocytes and ALT,LDH in preserved solutions were monitored and pathological changes of the preserved liver were observed after experiment.Results No significant changes were observed between group A and B.Instead,there are significant changes between group B and C,so were in groups C and D.Conclusion TET-HCA has optimization effect on HCA under the cold preservation on donor liver.During 2 hrs preservation period,similar effect was found between TET-HCA and UW solutions.
引文
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[12]丁国永,郭丽,杜忠君,等.骨髓间充质干细胞对肾缺血再灌注损伤后肾功能及氧化应激水平的改善作用[J].吉林大学学报:医学版,2009,35(4):587-590.
[2]陈保华,黄荣,车河龙,等.汉防已甲素不同给药方式对大鼠供肝能量代谢的影响[J].中国医院用药评价与分析,2011,11(8):717-718.
[3]陈保华,黄荣,姚斌,等.汉防已甲素对低温灌洗保存大鼠供肝的肝脏功能影响的实验研究[J].中国医院用药评价与分析,2011,11(9):814-816.
[4]赵建生,佟波涛,段加方,等.肝移植术中心跳骤停4例临床初探[J].东南国防医药,2009,11(4):326-328.
[5]Jamieson NV,Sundberg R,Landell S,et al.Preservation of the ca-nine liver for 24-28 hours using simple cold storage with UW solu-tin[J].Transplantation,1988,46(6):517-519.
[6]Chen XH,Hu YM,Liao YQ.Protective effect of tetrandrine onCCl4 atocytes[J].Acta Pharmacologica Sinica,1996,17(6):348-350.
[7]刘玉兰,李定国,陆汉明.汉防己甲素对肝细胞生长增殖的影响[J].上海第二医科大学学报,1995,15(3):212-215.
[8]范列英,孔宪涛.汉防己甲素对成纤维细胞及胶原合成的影[J].临床肝胆杂志,1995,11(8):25-26.
[9]阳文新,钟正江,申红,等.肝移植术后早期并发症的诊治(附44例报告)[J].东南国防医药,2005,7(3):169-207.
[10]王春战.姜黄素抗类抗氧化作用的研究进展[J].医学研究生学报,2012,25(6):658-660.
[11]Maiese K,Boniece I,DeMeo D,et al.Peptide grewth factors protectagainst ischemia in culture by prewvnting nitricoxide toxicity[J].Meurosci,1993,13(7):3034-3040.
[12]丁国永,郭丽,杜忠君,等.骨髓间充质干细胞对肾缺血再灌注损伤后肾功能及氧化应激水平的改善作用[J].吉林大学学报:医学版,2009,35(4):587-590.