独活肠吸收液对大鼠离体胸主动脉张力的影响
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摘要
目的:观察独活肠吸收液对大鼠离体胸主动脉环的舒张作用及其舒张机制。方法:应用外翻肠囊法制备独活含药肠吸收液,采用离体血管环技术制备大鼠胸主动脉环,使用累积加药法分别检测独活肠吸收液对PE、KCl刺激的内皮完整与去内皮胸主动脉环张力的影响,并探讨其Ca2+调节机制。结果:独活肠吸收液能够浓度依赖性地舒张PE、KCl引起的内皮完整与去内皮血管环收缩,内皮完整组与去内皮组最大舒张率比较差异无统计学意义。对PE或KCL预收缩的血管,独活肠吸收液均能抑制Ca Cl2所致最大收缩效应。结论:独活肠吸收液对PE、KCl引起的血管收缩均具有较好的舒张作用并呈剂量依赖性,其作用机制与抑制细胞外Ca2+内流密切相关。
Objective: To observe the vasodilatation effect of intestinal absorption solution of Angelica Pubescens on thoracic aorta and its related mechanism. Methods: Intestinal absorption solution of Angelica Pubescens was prepared by everted gut sac method in vitro. To investigate the influence and the calcium-related mechanisms of intestinal absorption solution of Angelica Pubescens on endothelium intact or endothelium denuded with PE and KCl. Results: Intestinal absorption solution of Angelica Pubescens could produce relaxation of thoracic aortic rings pre-contracted by PE and KCl with endothelium intact or denuded in a dose dependent manner. There was no significant difference between the endothelium intact group and the endothelium denuded group. Intestinal absorption solution of Angelica Pubescens inhibit Ca Cl2-induced maximum contraction effect. Conclusion: Intestinal absorption solution of Angelica Pubescens shows vasodilator effects on a dose-dependent manner. The vasodilatation effect is mediated by inhibition of extracellular Ca2 +influx.
Objective: To observe the vasodilatation effect of intestinal absorption solution of Angelica Pubescens on thoracic aorta and its related mechanism. Methods: Intestinal absorption solution of Angelica Pubescens was prepared by everted gut sac method in vitro. To investigate the influence and the calcium-related mechanisms of intestinal absorption solution of Angelica Pubescens on endothelium intact or endothelium denuded with PE and KCl. Results: Intestinal absorption solution of Angelica Pubescens could produce relaxation of thoracic aortic rings pre-contracted by PE and KCl with endothelium intact or denuded in a dose dependent manner. There was no significant difference between the endothelium intact group and the endothelium denuded group. Intestinal absorption solution of Angelica Pubescens inhibit Ca Cl2-induced maximum contraction effect. Conclusion: Intestinal absorption solution of Angelica Pubescens shows vasodilator effects on a dose-dependent manner. The vasodilatation effect is mediated by inhibition of extracellular Ca2 +influx.
引文
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[7]李振坤,刘玫琦,杨洪军,等.伞形科辛味中药主要化学成分对离体血管作用研究[J].中药药理与临床,2009,25(1):38-40.
[8]WILSONT H,WISEMAN G.The use of sacs of evened small intestine for the study of the transference of substances from the mucosal to the serosal surface[J].Physiology,1954,123(1):116.
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[10]姜建芳,沈晓兰,张宁.山楂总黄酮对大鼠离体血管功能的影响及其机制[J].中国现代应用药学,2011,28(7):614-617.
[2]邹玺,王瑞平,戴虹,等.独活抗血管生成作用的实验研究[J].南京中医药大学学报,2008,24(3):194-196.
[3]张知侠.短毛独活精油提取及抑菌作用的研究[J].咸阳师范学院学报,2007,22(2):33-34.
[4]卢永昌,张雪梅.独活抗氧化作用的研究[J].青海师专学报:教育科学,2004(5):74-76.
[5]李荣芷,孟娟知,何云庆,等.中药独活抗心律失常活性成分γ氨基丁酸的研究[J].北京医科大学学报,1989,21(5):376.
[6]李荣芷,何云庆,孟娟如,等.中药独活杭血小板聚集及抑制实验性血栓形成的活性成分研究[J].北京医科大学学报,1988(1):22.
[7]李振坤,刘玫琦,杨洪军,等.伞形科辛味中药主要化学成分对离体血管作用研究[J].中药药理与临床,2009,25(1):38-40.
[8]WILSONT H,WISEMAN G.The use of sacs of evened small intestine for the study of the transference of substances from the mucosal to the serosal surface[J].Physiology,1954,123(1):116.
[9]MANCINI GB,HENRY GC,MACAYA L,et al.Angiotensin converting enzyme inhibition,with quinapril,improves endothelial vasomotor dysfunction in patients with coronary artery disease:The TREND study(trial on reversing endothelial dysfunction)[J].Journal of the American College of Cardiology,1996,27(2):258-265.
[10]姜建芳,沈晓兰,张宁.山楂总黄酮对大鼠离体血管功能的影响及其机制[J].中国现代应用药学,2011,28(7):614-617.